Expression of the gene for the multidrug resistance-associated protein in human prostate tissue (original) (raw)
Abstract
To characterize the clinical relevance of MRP gene in the chemoresistance of prostate carcinomas we determined the multidrug resistance-associated protein (MRP) expression in 30 samples from organ-confined prostate carcinoma, 9 samples from adjacent normal tissue and 4 hormone unresponsive cancers. The measurement of MRP expression was carried out by reverse transcription polymerase chain reaction (RT-PCR) in combination with capillary electrophoresis. Incorporated fluorescence-labeled primers were disclosed by a laser-operated fluorescence detection module. MRP expression was quantified by integration of the peak area and correlated to the ubiquitously expressed β2 microglobulin. As positive control served the adriamycin-resistant HL60-ADR cell line, which overexpresses MRP. MRP expression was found in all samples. All samples showed a lower MRP/β2 ratio than HL60-ADR cells. The expression of the MRP gene was 30% higher in organ-confined tumors than in hormone-unresponsive anaplastic tumors. Normal tissue showed the same MRP mRNA level as the adriamycin-sensitive HL60 cells. A higher tumor stage correlated with an increase of MRP expression (>factor 2), whereas G3 tumors displayed a MRP expression 30% lower than in G2 tumors. The small alterations indicate that MRP expression seems not be involved in the chemoresistance of prostate carcinomas.
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Authors and Affiliations
- Institute of Pharmacology and Toxicology, Faculty for Clinical Medicine Mannheim, University of Heidelberg, Maybachstrasse 14–16, D-68169 Mannheim, Germany e-mail: schummer@rumms.uni-mannheim.de Tel.: 49 621 331067; Fax: 49 621 377601, , , , , , DE
Bernhard Schummer - Department of Urology, Faculty for Clinical Medicine Mannheim, University of Heidelberg, Germany, , , , , , DE
Michael Siegsmund, Anette Steidler, Lira Toktomambetova, Kai-Uwe Köhrmann & Peter Alken
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- Bernhard Schummer
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Received: 7 November 1997 / Accepted: 14 August 1998
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Schummer, B., Siegsmund, M., Steidler, A. et al. Expression of the gene for the multidrug resistance-associated protein in human prostate tissue.Urological Research 27, 164–168 (1999). https://doi.org/10.1007/s002400050104
- Issue Date: June 1999
- DOI: https://doi.org/10.1007/s002400050104