Detergent removal by non-polar polystyrene beads (original) (raw)
Abstract
Detergent removal from lipid-protein-detergent micellar solutions is the most successful strategy for reconstitution of integral membrane proteins into proteoliposomes or into two-dimensional crystals. This review establishes the potential of polystyrene beads as a simple alternative to other conventional detergent removing strategies such as dialysis, gel chromatography and dilution. Kinetics and equilibrium aspects of removal of different detergents by hydrophobic adsorption onto polystyrene beads have been systematically investigated. A mechanism of adsorption onto polystyrene beads is proposed and provide useful information about the use of these beads in reconstitution experiments. The usefulness of this detergent removal strategy to produce quasi-ideal proteoliposomes is evaluated by considering the morphology and the size of the reconstituted vesicles, the homogeneity in size and protein distribution, the final protein orientation and the permeability of resulting proteoliposomes. Finally, the advantages of detergent removal by polystyrene beads as an alternative to conventional dialysis in two-dimensional crystallization trials are evaluated through review of recent structural reconstitution studies.
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Authors and Affiliations
- Institut Curie, Section de Recherche, UMR-CNRS 168 and LRC-CEA 8, 11 Rue Pierre et Marie Curie, F-75231 Paris Cedex, France e-mail: rigaud@curie.fr., , , , , , FR
J.-L. Rigaud, Daniel Levy, Gervaise Mosser & Oliver Lambert
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- J.-L. Rigaud
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Received: 1 December 1997 / Revised version: 6 February 1998 / Accepted: 6 February 1998
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Rigaud, JL., Levy, D., Mosser, G. et al. Detergent removal by non-polar polystyrene beads .Eur Biophys J 27, 305–319 (1998). https://doi.org/10.1007/s002490050138
- Issue Date: June 1998
- DOI: https://doi.org/10.1007/s002490050138