The NF-κB binding site is essential for transcriptional activation of the IL-15 gene (original) (raw)

Abstract

We cloned the 5’ upstream region of IL-15 genomic DNA and examined promoter activity in macrophages stimulated with lipopolysaccharide(LPS). The 1.2 kilobase (kb) fragment of the 5’ upstream region contained binding elements for LPS-inducible transcription factors such as NFIL-6 or NF-κB. Determined by luciferase assay following transient transfection in the J774A.1 macrophage cell line, the 1.2 kb of the 5’ upstream region exhibited high promoter activity in response to LPS, while promoter activity was significantly reduced by the 5’ deletion of 313 base pairs containing the NF-κB binding motif. Nuclear protein prepared from LPS-stimulated macrophages formed a complex with the NF-κB binding sequence of the IL-15 promoter. Taken together, the binding of nuclear protein to the NF-κB binding site is required for transcriptional activation of the IL-15 gene in LPS-stimulated macrophages.

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Authors and Affiliations

1. Laboratory of Host Defense, Research Institute for Disease Mechanism and Control, and First Department of Surgery, Nagoya University School of Medicine, 65 Tsurumai-cho Showa-ku, Nagoya 466, Japan, , , , , , JP
   Junji Washizu, H. Nishimura, Nobuhisa Nakamura, Yuji Nimura & Yasunobu Yoshikai

Authors

1. Junji Washizu
2. H. Nishimura
3. Nobuhisa Nakamura
4. Yuji Nimura
5. Yasunobu Yoshikai

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Received: 26 August 1997 / Revised: 8 December 1997

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Washizu, J., Nishimura, H., Nakamura, N. et al. The NF-κB binding site is essential for transcriptional activation of the IL-15 gene.Immunogenetics 48, 1–7 (1998). https://doi.org/10.1007/s002510050393

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• Issue date: May 1998
• DOI: https://doi.org/10.1007/s002510050393