Heterologous gene expression of bovine plasmin in Lactococcus lactis (original) (raw)
Abstract
Heterologous production of bovine plasmin was studied in the industrially relevant bacterium Lactococcus lactis. Two sets of lactococcal gene expression signals were coupled to the region of the plasmin gene coding for the serine protease domain. When the promoter region of the prtP gene was used, plasmin was detected mainly intracellularly in strain BPL25 by Western blot hybridization. The intracellular presence of plasmin led to physiological stress. Expression of the plasmin gene driven by the promoter and complete signal sequence of the lactococcal usp45 gene resulted in efficient plasmin secretion in strain BPL420. Cell lysis was observed in strains producing plasmin fragments including the catalytic domain, but not in control strains, which only produced a non-catalytic region of plasmin. The plasmin produced was shown to be biologically active.
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Authors and Affiliations
- Biotechnological Institute, Anker Engelunds Vej 1, Building 227, DK-2800 Lyngby, Denmark Tel.: + 45 45 87 66 99, Fax: + 45 45 93 28 88 e-mail: arnau@biobase.dk, , , , , , DK
J. Arnau, E. Hjerl-Hansen & H. Israelsen
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- J. Arnau
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Received: 2 December 1996 / Received revision: 17 March 1997 / Accepted: 27 April 1997
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Arnau, J., Hjerl-Hansen, E. & Israelsen, H. Heterologous gene expression of bovine plasmin in Lactococcus lactis.Appl Microbiol Biotechnol 48, 331–338 (1997). https://doi.org/10.1007/s002530051058
- Issue Date: September 1997
- DOI: https://doi.org/10.1007/s002530051058