The colonization of a simulator of the human intestinal microbial ecosystem by a probiotic strain fed on a fermented oat bran product: effects on the gastrointestinal microbiota (original) (raw)

Abstract

The effects of _Lactobacillus_-GG-fermented oat bran product on the microbiota and its metabolic activity in the human gut were investigated, using a simulator of the human intestinal microbial ecosystem (SHIME), by analysing the bacterial population, short-chain fatty acids and gas production. In addition, the effects of fermented oat bran supernatant and supernatant samples from reactors 4, 5 and 6 (large intestine) on the growth of Escherichia coli IHE 13047, Enterococcus faecalis VTT E-93203, Lactobacillus rhamnosus VTT E-94522 (Lactobacillus GG) and Lactococcus lactis subsp. lactis VTT E-90414 were monitored to ascertain possible stimulatory/inhibitory effects by an in vitro turbidometric method. Our experiments showed that Lactobacillus GG colonized the SHIME reactor and this colonization could be maintained for several weeks without extra supplementation. Oat bran feeding also favoured the growth of bifidobacteria and caused an increase in the production of acetic, propionic and butyric acid as well as CH4 and CO2. However, the effects of oat bran, either on bacterial populations or on their metabolic activity, were not directly dose-dependent. In turbidometric measurements, the supernatant of fermented oat bran exerted an inhibitory effect of Lactobacillus GG, but stimulated the growth of enterococci.

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Authors and Affiliations

  1. VTT Biotechnology and Food Research, P.O. Box 1501, FIN-02044 VTT, Finland e-mail: Pia.Kontula@vtt.fi Tel.: +358 9 456 5998 Fax: +358 9 455 2028, , , , , , FI
    P. Kontula, J. Jaskari, A. von Wright, K. Poutanen & T. Mattila-Sandholm
  2. Laboratory of Microbial Ecology, Faculty of Agricultural and Applied Biological Sciences, University of Gent, Coupure Links 653, B-9000 Gent, Belgium, , , , , , BE
    L. Nollet & I. De Smet

Authors

  1. P. Kontula
  2. J. Jaskari
  3. L. Nollet
  4. I. De Smet
  5. A. von Wright
  6. K. Poutanen
  7. T. Mattila-Sandholm

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Received: 19 January 1998 / Received revision: 6 April 1998 / Accepted: 13 April 1998

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Kontula, P., Jaskari, J., Nollet, L. et al. The colonization of a simulator of the human intestinal microbial ecosystem by a probiotic strain fed on a fermented oat bran product: effects on the gastrointestinal microbiota.Appl Microbiol Biotechnol 50, 246–252 (1998). https://doi.org/10.1007/s002530051284

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