Sequence-ready physical map of the mouse Chromosome 16 region with conserved synteny to the human Velocardiofacial syndrome region on 22q11.2 (original) (raw)

Abstract.

Proximal mouse Chromosome (Chr) 16 shows conserved synteny with human Chrs 16, 8, 22, and 3. The mouse Chr 16/human Chr 22 conserved synteny region includes the DiGeorge/Velocardiofacial syndrome region of human Chr 22q11.2. A physical map of the entire mouse Chr 16/human Chr 22 region of conserved synteny has been constructed to provide a substrate for gene discovery, genomic sequencing, and animal model development. A YAC contig was constructed that extends ca. 5.4 Mb from a region of conserved synteny with human Chr 8 at Prkdc through the region conserved with human Chr 3 at DVL3. Sixty-one markers including 37 genes are mapped with average marker spacing of 90 kb. Physical distance was determined across the 2.6-Mb region from D16Mit74 to Hira with YAC fragmentation. The central region from D16Jhu28 to Igl-C1 was converted into BAC and PAC clones, further refining the physical map and providing sequence-ready template. The gene content and borders of three blocks of conserved linkage between human Chr 22q11.2 mouse Chr 16 are refined.

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Authors and Affiliations

  1. Physiology 205, Johns Hopkins University School of Medicine, 725 N. Wolfe St., Baltimore, Maryland 21205, USA, , , , , , US
    James Lund & Roger H. Reeves
  2. Dept. of Chemistry and Biochemistry, University of Oklahoma, Norman, Oklahoma 73019, USA, , , , , , US
    Bruce Roe
  3. Division of Human Genetics and Molecular Biology, The Children's Hospital of Philadelphia, Pennsylvania 19104, USA, , , , , , US
    Marcia Budarf & Beverly S. Emanuel
  4. The Wistar Institute, Philadelphia, Pennsylvania 19104, USA, , , , , , US
    Naomi Galili
  5. Torrey Pines Institute for Molecular Studies, San Diego, California 92121, USA, , , , , , US
    Roy Riblet
  6. Dept. of Biology, University of New Mexico, Albuquerque, New Mexico, USA, , , , , , US
    Robert D. Miller

Authors

  1. James Lund
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  2. Bruce Roe
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  3. Feng Chen
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  4. Marcia Budarf
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  5. Naomi Galili
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  6. Roy Riblet
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  7. Robert D. Miller
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  8. Beverly S. Emanuel
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  9. Roger H. Reeves
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Received: 4 November 1998 / Accepted: 21 December 1998

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Lund, J., Roe, B., Chen, F. et al. Sequence-ready physical map of the mouse Chromosome 16 region with conserved synteny to the human Velocardiofacial syndrome region on 22q11.2. 10, 438–443 (1999). https://doi.org/10.1007/s003359901019

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