Distribution of chromogranin B-like immunoreactivity in the human hippocampus and its changes in Alzheimer’s disease (original) (raw)

Abstract

Synapse loss is crucially involved in cognitive decline in Alzheimer’s disease (AD). This study was performed to investigate the distribution and density of chromogranin B-like immunoreactivity in the hippocampus of control compared to AD brain. Chromogranin B is a large precursor molecule found in large dense-core vesicles. For immunocytochemistry we used an antiserum raised against a synthetic peptide (PE-11) present in the chromogranin B molecule. Chromogranin B-like immunoreactivity was concentrated in the terminal field of mossy fibers, the inner molecular layer of the dentate gyrus and in layer II of the entorhinal cortex. In AD, chromogranin B was detected in neuritic plaques. The density of chromogranin B-like immunoreactivity was significantly reduced in the inner molecular layer of the dentate gyrus and in layers II, III and V of the entorhinal cortex in AD brains. The present study demonstrates that chromogranin B is a marker for human hippocampal pathways. It is particularly suitable for studying nerve fibers terminating at the inner molecular layer of the dentate gyrus. It is present in neuritic plaques, and its density is reduced in a layer-specific manner.

Access this article

Log in via an institution

Subscribe and save

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Author information

Authors and Affiliations

  1. Department of Psychiatry, University of Innsbruck, Anichstrasse 35, 6020 Innsbruck, Austria e-mail: j.marksteiner@uibk.ac.at, Tel.: +43-512-5043712, Fax: +43-512-5043713, , , , , , AT
    J. Marksteiner, T. Lechner, W. A. Kaufmann, P. Gurka, C. Humpel & C. Nowakowski
  2. Department of Pathology, University of Innsbruck, Austria, , , , , , AT
    H. Maier
  3. Ludwig-Boltzmann-Institute of Clinical Neurobiology, Vienna, Austria, , , , , , AT
    K. A. Jellinger

Authors

  1. J. Marksteiner
    You can also search for this author inPubMed Google Scholar
  2. T. Lechner
    You can also search for this author inPubMed Google Scholar
  3. W. A. Kaufmann
    You can also search for this author inPubMed Google Scholar
  4. P. Gurka
    You can also search for this author inPubMed Google Scholar
  5. C. Humpel
    You can also search for this author inPubMed Google Scholar
  6. C. Nowakowski
    You can also search for this author inPubMed Google Scholar
  7. H. Maier
    You can also search for this author inPubMed Google Scholar
  8. K. A. Jellinger
    You can also search for this author inPubMed Google Scholar

Additional information

Received: 14 February 2000 / Accepted: 6 March 2000

Rights and permissions

About this article

Cite this article

Marksteiner, J., Lechner, T., Kaufmann, W. et al. Distribution of chromogranin B-like immunoreactivity in the human hippocampus and its changes in Alzheimer’s disease.Acta Neuropathol 100, 205–212 (2000). https://doi.org/10.1007/s004010000239

Download citation