Bleomycin increases steady-state levels of type I collagen, fibronectin and decorin mRNAs in human skin fibroblasts (original) (raw)

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Abstract Bleomycin is a drug capable of inducing tissue fibrosis. In this study, the effects of bleomycin on gene expression of extracellular matrix encoding ·1(I) collagen, fibronectin and decorin were determined in vitro in human dermal fibroblasts. Northern blot analysis showed that bleomycin upregulated ·1(I) collagen, fibronectin and decorin gene expression dose-dependently between 1 n_M_ and 1 μ_M_. Bleomycin at 100 n_M_ upregulated α1(I) collagen, fibronectin and decorin mRNA expression with a peak at 6 h following stimulation in normal skin fibroblast monolayers. Bleomycin enhanced mRNA expression encoding these extracellular matrix proteins in both normal dermal and scleroderma fibroblasts. Concomitant stimulation with bleomycin and interferon-γ (1000 U/ml), a representative antifibrotic cytokine, decreased ·1(I) collagen mRNA expression. Bleomycin also mildly upregulated mRNA expression of transforming growth factor-‚ (TGF-‚) and connective tissue growth factor (CTGF) coordinately in normal skin fibroblasts. Our results indicate that bleomycin modulates gene expression of extracellular matrix proteins in dermal fibroblasts, and this effect may be mediated by TGF-‚ and CTGF.

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Authors and Affiliations

  1. Department of Dermatology, Tokyo Medical and Dental University, School of Medicine, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8519, Japan e-mail: yamamoto.derm@med.tmd.ac.jp, Tel.:+81-3-58035286, Fax:+81-3-58030143, , , , , , JP
    T. Yamamoto
  2. Department of Dermatology, University of Cologne, Joseph Stelzmann Str 9, 50924 Cologne, Germany, , , , , , DE
    Beate Eckes & Thomas Krieg

Authors

  1. T. Yamamoto
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  2. Beate Eckes
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  3. Thomas Krieg
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Received: 20 April 2000 / Revised: 19 June 2000 / Accepted: 4 September 2000

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Yamamoto, T., Eckes, B. & Krieg, T. Bleomycin increases steady-state levels of type I collagen, fibronectin and decorin mRNAs in human skin fibroblasts.Arch Dermatol Res 292, 556–561 (2000). https://doi.org/10.1007/s004030000180

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