Retinal degeneration induced by N-methyl-N-nitrosourea in Syrian golden hamsters (original) (raw)

Abstract

· Background: The sequential retinal changes in Syrian golden hamsters induced by _N_-methyl-_N_-nitrosourea (MNU) have not been studied. · Methods: Female hamsters received a single intraperitoneal injection of 90 mg/kg MNU at 50 days of age, and the retina was examined light and electron microscopically, immunohistochemically and by the TdT-mediated dUTP-digoxigenin nick end labeling (TUNEL) method until 20 weeks after the treatment. · Results: The retinal changes were as follows: (1) Photoreceptor apoptosis occurred 1 day after the treatment and resulted in photoreceptor loss at day 7. During the degeneration, Müller cell proliferation was conspicuous at day 5. (2) After the photoreceptor cell loss, migration of the pigment epithelial cells in all layers of the retina which were in contact with blood vessels occurred. Due to the Müller cell proliferation, gliosis was prominent at the later stage. · Conclusions: The MNU injection caused photoreceptor apoptosis followed by pigment epithelial cell migration around the blood vessels, accompanied by gliosis. The primary event and the course of this disease closely resemble those of retinitis pigmentosa in humans.

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Authors and Affiliations

  1. Department of Pathology, Kansai Medical University, Moriguchi, Osaka 570-8506, Japan Tel. +81-6-992-1001 ext. 2480; fax +81-6-992-5023, , , , , , JP
    Makoto Taomoto, Hiroyuki Nambu, Hideto Senzaki, Nobuaki Shikata & A. Tsubura
  2. Department of Ophthalmology, Kansai Medical University, Moriguchi, Osaka, Japan, , , , , , JP
    Hirohiko Miki & Masanobu Uyama
  3. Toxicology Research Laboratories, Fujisawa Pharmaceutical Co. Ltd., Yodogawa-ku, Osaka, Japan, , , , , , JP
    Yuji Oishi & Toshiyuki Fujii

Authors

  1. Makoto Taomoto
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  2. Hiroyuki Nambu
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  3. Hideto Senzaki
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  4. Nobuaki Shikata
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  5. Yuji Oishi
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  6. Toshiyuki Fujii
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  7. Hirohiko Miki
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  8. Masanobu Uyama
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  9. A. Tsubura
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Received: 6 August 1997 Revised version received: 26 November 1997 Accepted: 7 January 1998

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Taomoto, M., Nambu, H., Senzaki, H. et al. Retinal degeneration induced by _N_-methyl-_N-_nitrosourea in Syrian golden hamsters.Graefe's Arch Clin Exp Ophthalmol 236, 688–695 (1998). https://doi.org/10.1007/s004170050142

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