Molecular characterization of the Cryptosporidium cervine genotype from a sika deer (Cervus nippon Temminck) in Zhengzhou, China and literature review (original) (raw)

Abstract

A total of 124 fecal specimens were collected from four deer farms in Zhengzhou City, China and examined for Cryptosporidium by Sheather’s sugar flotation technique. Cryptosporidim oocysts were detected in two 1-year-old sika deer, and one of the two specimens was genotyped by sequence and phylogenetic analyses of the small subunit ribosomal RNA (rRNA) (18S rRNA), 70-kDa heat shock protein (HSP70), actin, and Cryptosporidium oocyst wall protein (COWP) genes. Results obtained suggested that the Cryptosporidium studied belonged to Cryptosporidium cervine genotype, although slight sequence differences were noticed at the three loci. The similarities between this isolate and other Cryptosporidium cervine genotype isolates were 99.1–99.8%, 9.8%, 99.7%, and 100% at the 18S rRNA, HSP70, actin, and COWP loci, respectively. This study is the first report of Cryptosporidium infection in sika deer in China.

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Acknowledgments

This study was supported in part by Henan Innovation Project for Prominent University Research Talents (number 2004KYCX002).

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Authors and Affiliations

1. The College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, People’s Republic of China
   Rongjun Wang, Jinchan Wang, Mingfei Sun, Hailiang Dang, Changshen Ning, Fuchun Jian & Longxian Zhang
2. School of Resource and Environmental Engineering, East China University of Science and Technology, Shanghai, 200237, People’s Republic of China
   Yaoyu Feng
3. US Department of Health and Human Services, Division of Parasitic Diseases, National Center for Zoonotic, Vector-Borne and Enteric Diseases, Centers for Disease Control and Prevention, Atlanta, GA, 30341, USA
   Lihua Xiao

Authors

1. Rongjun Wang
2. Jinchan Wang
3. Mingfei Sun
4. Hailiang Dang
5. Yaoyu Feng
6. Changshen Ning
7. Fuchun Jian
8. Longxian Zhang
9. Lihua Xiao

Corresponding authors

Correspondence toLongxian Zhang or Lihua Xiao.

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Wang, R., Wang, J., Sun, M. et al. Molecular characterization of the Cryptosporidium cervine genotype from a sika deer (Cervus nippon Temminck) in Zhengzhou, China and literature review.Parasitol Res 103, 865–869 (2008). https://doi.org/10.1007/s00436-008-1069-2

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• Received: 08 May 2008
• Accepted: 27 May 2008
• Published: 25 June 2008
• Issue date: September 2008
• DOI: https://doi.org/10.1007/s00436-008-1069-2

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