Identification and frequency distribution of Leishmania (L.) major infections in sand flies from a new endemic ZCL focus in southeast Iran (original) (raw)

Abstract

Zoonotic cutaneous leishmaniasis (ZCL) is an important public health challenge in Iran. It is often caused by the protozoan parasite, Leishmania major. This pathogen is principally transmitted by the infectious bites of adult female phlebotomine sand flies which belong to the subgenus Phlebotomus (Phlebotomus) Rondani and Berte (Diptera: Psychodidae). A recent outbreak of clinical ZCL cases in the rural district of Jask, Hormozgan province, southern Iran prompted the identification of sand flies naturally infected with L. major using polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA). A total of 8,123 wild sand flies were caught using sticky paper traps indoors and outdoors in seven villages of Jask from March 2007 to February 2008. About 120 trap-nights per month were carried out during peak seasonal density. Eight sand fly species of two distinct genera were morphologically identified. The most abundant species, Phlebotomus papatasi (≈60 %) and Phlebotomus salehi (17 %), had simultaneous population peaks in May and October. The anthropophilic index of P. papatasi was approximately twice that of P. salehi. PCR reactivity of L. major infections in P. salehi was weaker than those in P. papatasi. This is discussed with regard to their role in the natural transmission cycle of ZCL. This study provided the first PCR and ELISA evidence on P. salehi as a vector of L. major parasites in a new endemic region of ZCL in southeast Iran.

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Acknowledgments

The authors are deeply indebted to the vice-chancellor for research and technology at the Shiraz University of Medical Sciences (SUMS) for permitting the use of facilities at the university. The technical assistance by Ms. T. Dabaghmanesh in the provision of the regional map is acknowledged and much appreciated. This study was part of the research project number 24/P/15047 dated 27-2-2006, Hormozgan University of Medical Sciences. Their assistance in providing logistics and manpower is very much appreciated. The contents of this publication are the sole responsibility of the authors and do not necessarily reflect the views of the relevant institutions.

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No competing financial interests exist.

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Authors and Affiliations

1. Department of Medical Entomology, School of Health and Nutrition, Research Centre for Health Sciences, Shiraz University of Medical Sciences, P.O. Box: 71645-111, Shiraz, Iran
   Kourosh Azizi & Mohammad D. Moemenbellah-Fard
2. Department of Infectious Diseases, Imam Reza Hospital, School of Medicine, Mashhad University of Medical Sciences, P.O. Box: 91379-13131, Mashhad, Iran
   Farshid Abedi

Authors

1. Kourosh Azizi
2. Farshid Abedi
3. Mohammad D. Moemenbellah-Fard

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Correspondence toMohammad D. Moemenbellah-Fard.

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Azizi, K., Abedi, F. & Moemenbellah-Fard, M.D. Identification and frequency distribution of Leishmania (L.) major infections in sand flies from a new endemic ZCL focus in southeast Iran.Parasitol Res 111, 1821–1826 (2012). https://doi.org/10.1007/s00436-012-3029-0

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• Received: 20 July 2011
• Accepted: 27 June 2012
• Published: 11 July 2012
• Issue date: October 2012
• DOI: https://doi.org/10.1007/s00436-012-3029-0

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