A novel cDNA clone encoding a specific excretory/secretory antigen of larval Trichinella pseudospiralis (original) (raw)
Abstract
A cDNA library for Trichinella pseudospiralis was constructed to study the expression of specific antigens. Four positive clones were identified using antibodies against the excretory/secretory (ES) products of the nematode as probe. Sequence analysis showed that they contained identical cDNA inserts of 606 bp, including a 5′ non-translated region of 96 bp, a core translated segment of 408 bp and a poly(A)+ 3′ terminus. It encoded a novel 136-amino-acid polypeptide. Southern blot analysis indicated that the cDNA did not cross-hybridize to the genomic digests of T. spiralis, mouse, or rat. A single copy only of its complementary sequence was found in the genome of T. pseudospiralis. Using the lambda ZAP expression system, the cDNA was induced to express a 23-kDa β-galactosidase-fusion protein which did not cross-react with polyclonal and monoclonal antibodies against T. spiralis, heat shock proteins, or four heterologous species of nematodes. The antiserum against the fusion protein recognized a 15-kDa band from the ES products of T. pseudospiralis in immunoblotting. Immunocytolocalization demonstrated that the anti-fusion protein serum only recognized an epitope in the stichosome of T. pseudospiralis and not in T. spiralis. The protein can therefore serve as a specific antigen for the differential diagnosis of trichinellosis.
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Authors and Affiliations
- Department of Zoology, The University of Hong Kong, Hong Kong, China Fax: 852-2-5599114, e-mail: rcko@hkucc.hku.hk, , , , , , HK
Y. Y. Y. Chung & R. C. Ko
Authors
- Y. Y. Y. Chung
- R. C. Ko
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Received: 22 December 1998 / Accepted: 17 February 1999
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Chung, Y., Ko, R. A novel cDNA clone encoding a specific excretory/secretory antigen of larval Trichinella pseudospiralis.Parasitol Res 85, 685–691 (1999). https://doi.org/10.1007/s004360050616
- Issue date: June 1999
- DOI: https://doi.org/10.1007/s004360050616