Identification of a BIBAC clone that co-segregates with the petunia Restorer of fertility (Rf) gene (original) (raw)

Abstract.

Molecular markers closely linked to the Restorer of fertility (Rf) locus in petunia were sought by conducting a bulk segregant analysis. The co-segregation of markers and Rf was tested on a large BC1 population produced from two different parental lines carrying Rf. The recombination frequency between OP704 and ECCA/MACT, the two most distal markers utilized in the fine-scale mapping, was significantly different in populations derived from parents that carry different nuclear backgrounds. The fine mapping identified an amplified fragment length polymorphism (AFLP) marker that co-segregates with Rf. A petunia BIBAC library (four genome equivalents), with an average insert size of 70 kb, was constructed and screened with the linked marker. A contiguous map was constructed from three different BIBAC clones that hybridized to the marker. As a result, we have identified a 37.5-kb BIBAC clone that co-segregates with Rf.

Access this article

Log in via an institution

Subscribe and save

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Author information

Authors and Affiliations

  1. Department of Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca, NY 14853, USA, , , , ,
    S. Bentolila & M. Hanson

Authors

  1. S. Bentolila
    You can also search for this author inPubMed Google Scholar
  2. M. Hanson
    You can also search for this author inPubMed Google Scholar

Additional information

Electronic Publication

Rights and permissions

About this article

Cite this article

Bentolila, .S., Hanson, .M. Identification of a BIBAC clone that co-segregates with the petunia Restorer of fertility (Rf) gene.Mol Gen Genomics 266, 223–230 (2001). https://doi.org/10.1007/s004380100544

Download citation