NME6: a new member of the nm23/nucleoside diphosphate kinase gene family located on human chromosome 3p21.3 (original) (raw)

Abstract

The NME (nm23/nucleoside diphosphate kinase) gene family in human is involved in the phosphorylation of nucleoside diphosphates and a variety of regulatory phenomena associated with development, oncogenic transformation, and metastasis. Here we report the cDNA sequence for a sixth member of this family, NME6. The cDNA sequence predicts a 186-residue protein that includes the characteristic active site motif of a nucleoside diphosphate (NDP) kinase, as well as the other residues previously identified as crucial for nucleotide binding and catalysis. The NME6 protein sequence is only 34–41% identical to the five previously reported human NME proteins, and is similarly related to prokaryotic and primitive eukaryotic NDP kinases. Compared to typical proteins of this family such as NME1 and NME2, NME6 has three additional residues located in the Kpn loop, and a 22-residue extension at the COOH-terminal. Using radiation hybrid mapping, the NME6 gene was localized to chromosome 3p21.3. The 1.3-kb transcript of NME6 is expressed at a moderately low level in many human tissues, and is most abundant in kidney, prostate, ovary, intestine, and spleen. Homologous cDNAs were also cloned and sequenced for rat and mouse. The sequence of the first 171 residues of the mouse homologue (Nm23-M6) is 94% identical to the deduced human NME6 protein.

Access this article

Log in via an institution

Subscribe and save

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Author information

Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, University of North Dakota School of Medicine and Health Sciences, Grand Forks, ND 58202, USA, e-mail: dlambeth@medicine.nodak.edu, Tel.: +1-701-7772759, Fax: +1-701-7772382, , , , , , US
    James G. Mehus & D. O. Lambeth
  2. The Sanger Centre, Hinxton Hall, Hinxton, Cambridge CB10 1SA, UK, , , , , , GB
    Panagiotis Deloukas

Authors

  1. James G. Mehus
  2. Panagiotis Deloukas
  3. D. O. Lambeth

Additional information

Received: 16 March 1999 / Accepted: 29 March 1999

Rights and permissions

About this article

Cite this article

Mehus, J., Deloukas, P. & Lambeth, D. NME6: a new member of the nm23/nucleoside diphosphate kinase gene family located on human chromosome 3p21.3.Hum Genet 104, 454–459 (1999). https://doi.org/10.1007/s004390050987

Download citation

Keywords