Ontogeny of renal sulfate transporters: postnatal mRNA and protein expression (original) (raw)
Abstract
Renal reabsorption of inorganic sulfate changes during growth and development. We have studied the expression of two proximal tubular sulfate transporters, NaSi-1 and sat-1, during postnatal maturation. Both NaSi-1 and sat-1 mRNA levels increase postnatally, peaking at day 14, with a maximal tenfold increase in NaSi-1 and sat-1 mRNA levels compared with day 1. A similar age-dependent increase was observed for sodium-dependent and sodium-independent sulfate uptakes by injection of rat kidney mRNA into Xenopus oocytes. Western blot analysis of renal membranes from rats aged day 1 to day 77 showed no significant changes for NaSi-1 protein, whereas sat-1 protein levels paralleled mRNA expression, increasing from day 1 to day 14, followed by a decrease in protein levels thereafter. For NaSi-1 protein expression, translational or posttranslational mechanisms may maintain equal numbers of sulfate proteins on proximal tubular membranes during maturation, whereas sat-1 protein levels are in close agreement with mRNA changes. This is the first study to look at the ontogeny of renal sulfate transporter (NaSi-1 and sat-1) expression during postnatal maturation.
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Authors and Affiliations
- Department of Physiology and Pharmacology, The University of Queensland, Brisbane, Queensland 4072, Australia e-mail: danielm@plpk.uq.edu.au Tel.: +61-7-3365-1400, Fax: +61-7-3365-1766, , , , , , AU
D. Markovich & Tania S. Fogelis
Authors
- D. Markovich
- Tania S. Fogelis
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Received: 16 December 1998 / Revised: 15 April 1999 / Accepted: 20 April 1999
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Markovich, D., Fogelis, T. Ontogeny of renal sulfate transporters: postnatal mRNA and protein expression.Pediatr Nephrol 13, 806–811 (1999). https://doi.org/10.1007/s004670050705
- Issue date: November 1999
- DOI: https://doi.org/10.1007/s004670050705