Renal epithelial cell injury and its promoting role in formation of calcium oxalate monohydrate (original) (raw)

Abstract

The injurious effect of hydrogen peroxide (H2O2) on renal epithelial cells of the African green monkey (Vero cells) and the difference in the modulation of Vero cells on crystal growth of calcium oxalate (CaOxa) before and after injury were investigated. The degree of injury of Vero cells was proportional to the concentration and action time of H2O2. After the cells had been injured, the released amount of malonaldehyde in the culture medium increased, the superoxide dismutase activity decreased, the expression quantity of osteopontin on the surface of Vero cells increased significantly, the zeta potential became more negative, and the amount of CaOxa crystals adhering to cells increased. The CaOxa crystals induced by the cells in the control group were round and blunt; however, those induced by the injured cells had irregular shapes with sharp edges and corners. As the crystallization time increased from 6 to 24 h, the size of the crystals induced by the injured cells increased accordingly, whereas that of crystals induced by the control cells did not increase significantly. The injured cells could promote the growth of CaOxa crystals and their adhesion to the cells; thus, the formation of CaOxa stones was promoted. The cells in the control group could also be injured after being incubated with supersaturated CaOxa solution for a long time, which promoted the crystallization of CaOxa. The results suggest that the retention of supersaturated CaOxa solution or CaOxa crystals in the urinary tract for a long time is a risk factor for the formation of kidney stones.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (20971057), and the project sponsored by the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education of China. We thank Jiang Jian-Wei from the Medical College of Jinan University, Guangzhou, China, for technical assistance in cell culture.

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Authors and Affiliations

  1. Department of Chemistry, Jinan University, Guangzhou, 510632, China
    Jian-Ming Ouyang, Xiu-Qiong Yao, Jin Tan & Feng-Xin Wang
  2. Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou, 510632, China
    Jian-Ming Ouyang, Xiu-Qiong Yao, Jin Tan & Feng-Xin Wang

Authors

  1. Jian-Ming Ouyang
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  2. Xiu-Qiong Yao
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  3. Jin Tan
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  4. Feng-Xin Wang
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Correspondence toJian-Ming Ouyang.

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Ouyang, JM., Yao, XQ., Tan, J. et al. Renal epithelial cell injury and its promoting role in formation of calcium oxalate monohydrate.J Biol Inorg Chem 16, 405–416 (2011). https://doi.org/10.1007/s00775-010-0738-7

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