Rapid urine preparation prior to identification of uropathogens by MALDI-TOF MS (original) (raw)

Abstract

Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-ToF MS) has been introduced in clinical routine microbiology laboratories. For the rapid diagnosis of urinary tract infections, culture-independent methods prior MALDI-mediated identification have been described. Here, we describe a comparison of three of these methods based on their performance of bacterial identification and their potential as a routine tool for microbiology labs : (i) differential centrifugation, (ii) urine filtration and (iii) a 5-h bacterial cultivation on solid culture media. For 19 urine samples, all methods were directly compared and correct bacterial species identification by MALDI was used as performance indicator. A higher percentage of correct MALDI identification was obtained after filtration (78.9 %) and the growth-based method (84.2 %) as compared to differential centrifugation (68.4 %). Additional testing of 76 mono-microbial specimens (bacteriuria > 105 CFU/mL) confirmed the good performance of short growth with a 90.8 % correct MALDI score, with a potentially better fit to the routine workflow of microbiology labs.

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Acknowledgments

We gratefully acknowledge Valérie Coste and Nadia Piga from the bioMérieux sample bank team for the samples supply contact, also Professor Thierry Delair for reviewing the manuscript and Maud Arsac for support in data processing.

Conflict of interest

The authors (except A. Lesenne and A. Richez) are employees of bioMérieux, a company creating and developing infectious disease diagnostics, and in particular the VITEK® MS system involved in this study. No further potential conflicts of interest relevant to this article are reported.

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Authors and Affiliations

  1. Innovation/Technology Research Department, bioMérieux S.A., Chemin de l’Orme, 69280, Marcy L’Etoile, France
    L. Veron, B. H. Muller, G. L’Hostis, C. Ducruix, H. Rostaing, V. Lanet & F. Mallard
  2. Microbiology R&D, bioMérieux S.A., 38390, La Balme Les Grottes, France
    S. Mailler, V. Girard, S. Ghirardi & A. van Belkum
  3. Novescia, Paris-Sud, 91320, Wissous, France
    A. Lesenne
  4. Medical Biology Laboratory, St-Luc/St-Joseph, Hospices Civils de Lyon, 69007, Lyon, France
    A. Richez

Authors

  1. L. Veron
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  2. S. Mailler
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  3. V. Girard
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  4. B. H. Muller
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  5. G. L’Hostis
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  6. C. Ducruix
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  7. A. Lesenne
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  8. A. Richez
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  9. H. Rostaing
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  10. V. Lanet
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  11. S. Ghirardi
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  12. A. van Belkum
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  13. F. Mallard
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Corresponding author

Correspondence toL. Veron.

Electronic supplementary material

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Table A1

MALDI-TOF MS-mediated identification scores after differential centrifugation. (DOC 48 kb)

Table A2

MALDI identification results for different culture times. (DOC 48 kb)

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Veron, L., Mailler, S., Girard, V. et al. Rapid urine preparation prior to identification of uropathogens by MALDI-TOF MS.Eur J Clin Microbiol Infect Dis 34, 1787–1795 (2015). https://doi.org/10.1007/s10096-015-2413-y

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