Genetic Relatedness and Variability in Inbred and Wild Populations of the Solitary Ascidian Ciona intestinalis Revealed by Arbitrarily Primed Polymerase Chain Reaction (original) (raw)
Abstract:
As the first step in the establishment of inbred Ciona intestinalis strains, the genetic backgrounds of several local populations were evaluated on the basis of comparing 313 random amplified polymorphic DNA (RAPD) loci. Cluster analyses of individuals indicated that this species has local genetic characteristics, although various genetic distances among the populations were relatively low. The bulked RAPD analyses revealed that populations from Torihama and from Onagawa were the most distantly related among the 7 populations examined, suggesting that these two populations are the best candidates for outbreeding. Successive inbreeding in the laboratory was achieved using the Onagawa population. Although the genetic similarity of the F4 progeny became distinctly higher than that of natural populations, F5 progeny did not survive, presumably owing to homozygous lethal alleles.
Access this article
Subscribe and save
- Get 10 units per month
- Download Article/Chapter or eBook
- 1 Unit = 1 Article or 1 Chapter
- Cancel anytime Subscribe now
Buy Now
Price excludes VAT (USA)
Tax calculation will be finalised during checkout.
Instant access to the full article PDF.
Similar content being viewed by others
Author information
Authors and Affiliations
- Department of Zoology, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan, , , , , , JP
Shungo Kano, Shota Chiba & Noriyuki Satoh
Authors
- Shungo Kano
You can also search for this author inPubMed Google Scholar - Shota Chiba
You can also search for this author inPubMed Google Scholar - Noriyuki Satoh
You can also search for this author inPubMed Google Scholar
Additional information
Received October 15, 1999; accepted July 18, 2000.
Rights and permissions
About this article
Cite this article
Kano, S., Chiba, S. & Satoh, N. Genetic Relatedness and Variability in Inbred and Wild Populations of the Solitary Ascidian Ciona intestinalis Revealed by Arbitrarily Primed Polymerase Chain Reaction.Mar. Biotechnol. 3, 58–67 (2001). https://doi.org/10.1007/s101260000048
- Issue Date: January 2001
- DOI: https://doi.org/10.1007/s101260000048