Lipofuscin Component A2E Does Not Reduce Antioxidant Activity of DOPA-Melanin (original) (raw)

We studied the capacity of DOPA-melanin (natural eumelanin analog) to bind chromophore A2E of retinal pigmented epithelial cell lipofuscin granule into complexes. DOPA-melanin bound up to 200 nm A2E per 1 mg polymer; antioxidant activity of the resultant complexes was evaluated. Luminol chemiluminescence quenching in the presence of hydrogen peroxide showed that the chemiluminescence latency/concentration constants were virtually the same for DOPA-melanin and its A2E complexes. Comparison of the inhibitory effects of DOPAmelanin and DOPA-A2E complexes by rate of UV-induced peroxidation of the outer segments of photoreceptor cells showed higher inhibitory activity of the complexes in comparison with pure DOPA-melanin. Antioxidant activity of DOPA-A2E complexes towards Fe2+-ascorbateinduced peroxidation of the outer segments of photoreceptor cells was also higher than that of DOPA-melanin. The results indicated that chromophore A2E of lipofuscin granules in the studied concentrations did not attenuate the antioxidant effects of DOPA-melanin and even potentiated it. This suggested that A2E excess in retinal pigmented epithelium cells could be bound by melanosome melanin and lose its toxicity.

Access this article

Log in via an institution

Subscribe and save

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. G. I. Klebanov, Yu. O. Teselkin, I. V. Babenkova I. V., et al., Vestn. Rossiisk. Akad. Med. Nauk, No. 2, 15–22 (1999).
  2. J. A. Buege and S. D. Aust, Methods Enzymol., 52, 302–310 (1978).
    Article PubMed CAS Google Scholar
  3. M. A. Ostrovsky, N. L. Sakina, and A. E. Dontsov, Vision Res., 27, No. 6, 893–899 (1987).
    Article PubMed CAS Google Scholar
  4. C. A. Parish, M. Hashimoto, K. Nakanishi, et al., Proc. Natl. Acad. Sci. USA, 95, No. 25, 14,609-14,613 (1998).
    Article CAS Google Scholar
  5. H. G. Smith, G. W. Stubbs, and B. J. Litman, Exp. Eye Res., 20, No. 3, 211–217 (1975).
    Article PubMed Google Scholar
  6. J. R. Sparrow, Y. Wu, C. Y. Kim, and J. Zhou, J. Lipid Res., 51, No. 2, 247–261 (2010).
    Article PubMed CAS Google Scholar
  7. J. R. Sparrow, H. R. Vollmer-Snarr, J. Zhou, et al., J. Biol. Chem., 278, No. 20, 18,207-18,213 (2003).
    Article CAS Google Scholar
  8. K. B. Stepien, J. P. Dworzanski, B. Bilinska, et al., Biochim. Biophys. Acta, 997, Nos. 1–2, 49–54 (1989).
    Article PubMed CAS Google Scholar
  9. Z. Wang, J. Dillon, and E. R. Gaillard, Photochem. Photobiol., 82, No. 2, 474–479 (2006).
    Article PubMed CAS Google Scholar

Download references

Author information

Authors and Affiliations

  1. N. M. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, Russia
    A. E. Dontsov, A. D. Koromyslova & N. L. Sakina

Authors

  1. A. E. Dontsov
    You can also search for this author inPubMed Google Scholar
  2. A. D. Koromyslova
    You can also search for this author inPubMed Google Scholar
  3. N. L. Sakina
    You can also search for this author inPubMed Google Scholar

Corresponding author

Correspondence toN. L. Sakina.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 154, No. 11, pp. 579-582, November, 2012

Rights and permissions

About this article

Cite this article

Dontsov, A.E., Koromyslova, A.D. & Sakina, N.L. Lipofuscin Component A2E Does Not Reduce Antioxidant Activity of DOPA-Melanin.Bull Exp Biol Med 154, 624–627 (2013). https://doi.org/10.1007/s10517-013-2015-6

Download citation

Key Words