New isolate of Streptomyces sp. with novel thermoalkalotolerant cellulases (original) (raw)

Abstract

A Streptomyces sp. was isolated that produced novel thermoalkalotolerant cellulase activity after growth on crystalline cellulose at 50°C. Three major components of the cellulases (CMCase, Avicelase and cellobiase) were produced with maximal activities (11.8, 7.8 and 3.9 IU/ml) and maximum specific activities 357, 276 and 118 IU/mg protein, respectively, after 120 h growth. Maximum CMCase activity was between 50 and 60°C measured over 3 h. The enzyme also retained 88% of its maximum activity at 70°C and pH 5, and 80% of the activity at pH 10 and 50°C when assayed after 1 h. After incubation at 40°C for 1 h with commercial detergent (Tide) at pH 11, 95% activity was retained. The enzyme mixture produced glucose from crystalline cellulose.

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Acknowledgments

Portions of this research were funded through the Natural Sciences and Engineering Research Council of Canada.

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Authors and Affiliations

1. Biochemical Engineering Group, Department of Chemical Engineering, University of Waterloo, Waterloo, ON, Canada, N2L 3G1
   Faiez Alani, William A. Anderson & Murray Moo-Young

Authors

1. Faiez Alani
2. William A. Anderson
3. Murray Moo-Young

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Correspondence toWilliam A. Anderson.

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Alani, F., Anderson, W.A. & Moo-Young, M. New isolate of Streptomyces sp. with novel thermoalkalotolerant cellulases.Biotechnol Lett 30, 123–126 (2008). https://doi.org/10.1007/s10529-007-9500-9

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• Received: 06 February 2007
• Revised: 12 July 2007
• Accepted: 16 July 2007
• Published: 31 July 2007
• Issue date: January 2008
• DOI: https://doi.org/10.1007/s10529-007-9500-9

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