Does radial nuclear organisation influence DNA damage? (original) (raw)
Abstract
It has been suggested that chromatin at the nuclear periphery could act to shield DNA sequences in the nuclear interior from damage. To test this hypothesis, we have examined the nuclear distribution of sites of DNA repair induced by oxidation or UV-C. We do not detect more damage (repair) at the nuclear periphery than in the nuclear interior. In fact, contrary to the bodyguard hypothesis, there is an excess of damage detected in the nuclear interior. This is further supported by sequence comparison between genes on human chromosomes 18 or 19, and their counterparts in the chimpanzee. The synonymous substitution rate for genes on chromosome 19, which is located towards the centre of the human nucleus, was higher than that for genes on chromosome 18, which is located at the nuclear periphery. We conclude that chromatin at the periphery of the human nucleus is not able to protect more internally located sequences from damage and mutation. We suggest that features of the chromatin structure, or base composition, of sequences in the nuclear centre make them more susceptible to damage.
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Author notes
- Elodie Gazave
Present address: Departament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra, Doctor Aiguader, 80, 08003, Barcelona, Spain
Authors and Affiliations
- MRC Human Genetics Unit, Crewe Road, Edinburgh, EH4 2XU, UK
Elodie Gazave, Philippe Gautier, Susan Gilchrist & Wendy A. Bickmore
Authors
- Elodie Gazave
You can also search for this author inPubMed Google Scholar - Philippe Gautier
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Correspondence toWendy A. Bickmore.
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Gazave, E., Gautier, P., Gilchrist, S. et al. Does radial nuclear organisation influence DNA damage?.Chromosome Res 13, 377–388 (2005). https://doi.org/10.1007/s10577-005-3254-9
- Received: 18 January 2005
- Accepted: 28 February 2005
- Issue Date: June 2005
- DOI: https://doi.org/10.1007/s10577-005-3254-9