Androgenetic haploids and SSR markers as tools for the development of tropical maize hybrids (original) (raw)

Abstract

The traditional process of obtaining maize hybrids involves the generation of inbred lines through successive generations of selfing and subsequent testcrosses in order to identify the best combining ability by allelic complementation. A fast alternative to obtain inbred lines is to induce the formation of haploids followed by chromosome doubling. However, even with the aid of haploid-inducing genetic sources, this strategy has not been widely used in maize breeding programs, partly due to difficulties inherent to haploid generation and identification. In order to evaluate the possibility of using dihaploids to generate homozygous maize tropical lines, we used the androgenetic haploid inducer line W23 as a female parent in crosses with the tropical single-cross hybrid BRS1010. Within the progeny of these crosses, 462 seeds were phenotypically selected as putative haploids by the purple-colored endosperm and colorless embryo conditioned by the R1-nj gene. Among these, only four individuals were confirmed as being haploids using SSR markers, chromosome counting and flow cytometry, showing that the phenotypic marker was not efficient in detecting haploids in the tropical maize genotype used. All four haploids as well as some diploid plants presented reduced size, corroborating the difficulties for haploid identification by phenotypic evaluation. Genetic diversity analysis revealed by SSR markers divided the haploids in two groups represented by flint and dent maize inbred lines, which could be helpful in identifying complementary dihaploid lines. The present article demonstrates that a combination of haploid production and SSR fingerprinting is a feasible strategy for maize hybrid development in tropical germplasm.

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Abbreviations

Ig :

indeterminate gametophyte

R1-nj :

R-navajo

SSR:

Simple Sequence Repeat

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Acknowledgments

The authors thank Jurandir Vieira de Magalhães and Lyza Maron for comments and fruitful suggestions on the manuscript. We also thank Dr Carlos Roberto de Carvalho for technical assistance on flow cytometry analysis. The project was supported by Embrapa Maize and Sorghum, Brazilian Council of Research and Scientific Development - CNPq, Foundation of Research Support of Minas Gerais State - FAPEMIG, and CCRP McKnight Foundation. P.R.B received fellowship from CAPES, Brazil. E.P and C.T.G were recipients of a research fellowship from CNPq, Brazil.

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Authors and Affiliations

1. Federal University of Lavras, P.O. Box 3037, Lavras, 37200-000, MG, Brazil
   Pedro Radi Belicuas, Luciano Vilela Paiva & Wilson Roberto Maluf
2. Embrapa Maize and Sorghum, P.O. Box 151, Sete Lagoas, 35701-970, MG, Brazil
   Claudia Teixeira Guimarães & Edilson Paiva
3. Syngenta Seeds, P.O. Box 585, Uberlândia, 38400-974, MG, Brazil
   Jair Moura Duarte

Authors

1. Pedro Radi Belicuas
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2. Claudia Teixeira Guimarães
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3. Luciano Vilela Paiva
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4. Jair Moura Duarte
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5. Wilson Roberto Maluf
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6. Edilson Paiva
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Correspondence toEdilson Paiva.

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Belicuas, P.R., Guimarães, C.T., Paiva, L.V. et al. Androgenetic haploids and SSR markers as tools for the development of tropical maize hybrids.Euphytica 156, 95–102 (2007). https://doi.org/10.1007/s10681-007-9356-z

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• Received: 15 July 2005
• Accepted: 06 January 2007
• Published: 21 February 2007
• Issue Date: July 2007
• DOI: https://doi.org/10.1007/s10681-007-9356-z

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