Genetic diversity and differentiation of Camellia sinensis L. (cultivated tea) and its wild relatives in Yunnan province of China, revealed by morphology, biochemistry and allozyme studies (original) (raw)

Abstract

We evaluated morphological, isozyme and biochemical diversity of a total of 87 accessions in the genus Camellia [Camellia sinensis var. sinensis (10), C. talinensis (7), C. sinensis var. dehungensis (3), C. crassicolumna (3) and C. sinensis var. assamica (64)]. Great variation of morphological characters was apparent within each taxa. Across the five taxa, all leaf and most flower quantitative characters showed significant differences while all fruit quantitative characters measured did not differ significantly, and, seven (i.e., life form, bud color, petal texture, pubescence on ovary, style number, stamen location and locule per fruit) of the 33 qualitative characters yield significant differences. As a whole, caffeine content had the highest variation with CV of 22.7%, water extract solid showed the least variation (13.4%) and content of polyphenols (20.0%) and free amino acids (18.8%) showed intermediate variations. Camellia taliensis and C. sinensis var. assamica had significantly higher content of polyphenols and water extract solid than in the other three taxa, while no significant differences were detected for the content of caffeine and free amino acids. For allozyme study, 14 loci presented good resolution, among which, nine loci (64%) were polymorphic in each taxon (AAT-3, FUM-1, 6PDG-1, G6PDH-1, G3PDH-1, ME-1, PGM-1, PGM-2 and SKD-1). The percentage of polymorphic loci (P) for each taxon was 21.4–50.0%. Mean heterozygosity per locus (H e ) varied 0.114–0.218. F ST value indicated that only 4.6% of the variations could be ascribable to genetic differences among taxa. Genetic relationships among the five taxa revealed by allozymes, were also exposed by the result of clustering of the morphological and biochemical characters.

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References

• Banerjee B. 1992. Botanical classification of tea. In: Wilson K.C. and Clifford M.N. (eds), Tea: Cultivation to Consumption, Chapman and Hall, London, pp. 25–51.
  Google Scholar
• Chang H.T. and Bartholomew B. 1984. Camellia. B.T. Batsford, London.
  Google Scholar
• Chen X.Y. 1994. The Original Locality of Tea Plant. Yunnan People’s Press, Kunming Yunnan, China (in Chinese).
  Google Scholar
• Doebley J. 1989. Isozymic evidence and the evolution of crop plants. In: Soltis D.E. and Soltis P.S. (eds), Isozymes in Plant Biology, Dioscorides, Portland, OR, pp. 165–191.
  Google Scholar
• Du Q.Z., Li M.J. and Liu W.H. 1990. Chemical and numerical taxonomies of plants Thea section plants. J. Tea Sci. 10: 1–12 (in Chinese with English abstract).
  Google Scholar
• Evans J.C. 1992. Tea in China. Greenwood Press, 88 Post Road West, Westport, pp. 1–22.
  Google Scholar
• Hebert P.D.N. and Beaton M.J. 1993. Methodologies for Allozyme Analysis using Cellulose Acetate Electrophoresis: A practical Handbook. (An educational service of Helena Laboratories).
• Ikeda N., Kawada M. and Takeda Y. 1991. Isozymic analysis of Camellia sinensis and its interspecific hybrids. In: Proceedings of the International Symposium of Tea Science, held at Shizouka, Japan, 26–28 August 1991, pp. 98 (Abstract).
• Kaundun S.S. and Park Y.G. 2002. Genetic structure of six Korean tea populations as revealed by RAPD-PCR markers. Crop Sci. 42: 594–601.
  Article CAS Google Scholar
• Kaundun S.S., Zhyvoloup A. and Park Y.G. 2000. Evaluation of the genetic diversity among elite tea (Camellia sinensis var. sinensis) accessions using RAPD markers. Euphytica 115: 7–16.
  CAS Google Scholar
• Lai J.A., Yang W.C. and Hsiao J.Y. 2001. An assessment of genetic relationships in cultivated tea clones and native wild tea in Taiwan using RAPD and ISSR maskers. Bot. Bull. Acad. Sinica 42: 93–100.
  CAS Google Scholar
• Lee S.H., Choi H.S., Kim R.S., Lee H.Y. and Nou I.S. 1995. Identification of Korean wild tea plants and Japanese green tea cultivars using RAPD markers. J. Korean Tea Sci. 1: 129–148.
  Google Scholar
• Levin D.A. 2002. Hybridization and extinction. Am. Sci. 90: 254–261.
  Google Scholar
• Li H.L. 1983. The domestication of plants in China: ecogeographical considerations. In: Keightley D.N. (ed.), The Origins of Chinese Civilization, University of California Press.
• Lu C.Y., Liu W.H. and Li M.J. 1992. Relationship between the evolutionary relatives and the variation of esterase isozymes in tea plant. J. Tea Sci. 12: 15–20 (in Chinese with English abstract).
  Google Scholar
• Magoma G.N., Wachira F.N., Obanda M., Imbuga M. and Agong S.G. 2000. The use of catechins as biochemical markers in diversity studies of tea (Camellia sinensis). Genet. Resour. Crop Evol. 47: 107–114.
  Google Scholar
• Matsumoto S., Takeuchi A., Hayatsu M. and Kondo S. 1994. Molecular cloning of phenylalanine ammonia lyase cDNA and classification of varieties and cultivars of tea plants (Camellia sinensis) using the tea PAL cDNA probe. Theor. Appl. Genet. 89: 671–675.
  CAS Google Scholar
• Ming T.L. 2000. Monograph of the Genus Camellia. Yunnan Science and Technology Press, Kunming, Yunnan, China, pp. 110–135 (in Chinese with English abstract).
  Google Scholar
• Nei M. 1978. Estimation of average heterozygosity and genetic distance from a small number of indivisuals. Genetics 89: 583–590.
  CAS PubMed Google Scholar
• Paul S., Wachira F.N., Powell W. and Waugh R. 1997. Diversity and genetic differentiation among populations of Indian and Kenyan tea (Camellia sinensis (L.) O. Kuntze) revealed by AFLP markers. Theor. Appl. Genet. 94: 255–263.
  CAS Google Scholar
• Sao W.F. and Sheng P.H. 1993. The brief history and characteristic of Pu’er Tea in Yunnan province. Chinese Agr. Archaeol. (supplement issue for Chinese tea culture), 50–54 (in Chinese).
• Sealy J. 1958. A Revision of the Genus Camellia. Royal Horticultural Society, London.
  Google Scholar
• Song W.X. and Li D.P. 1990. Din Hai Yu Heng Zhi Jiao Zhu. Yunnan People’s Press, Kunming, Yunnan, China (in Chinese).
  Google Scholar
• Swofford D.L. and Selander R.B. 1989. BIOSYS-1: a computer program for the analysis of allelic variation in population genetics and biochemical systematics. Illinois Natural History Survey, Champaign, IL, USA.
  Google Scholar
• Takeda Y. 1990. Cross compatibility of tea (Camellia sinensis) and its allied species in the genus Camellia. Jpn. Agrric. Res. Q. 24: 111–116.
  Google Scholar
• Takeda Y. 1994. Differences in caffeine and tannin contents between tea cultivars and application to tea breeding. Jpn. Agric. Res. Q. 28: 117–123.
  CAS Google Scholar
• Toyao T. and Takeda Y. 1999. Studies on geographical diversity of floral morphology of tea plant (Camellia sinensis (L.) O. Kuntze) using the method of numerical taxonomy. Tea Res. J. 87: 39–57.
  Google Scholar
• TLPMBand ITP (Tea and Livestock Products Management Bureau and Institute of Tea Processing, the Ministry of Commerce), 1989. The Physiochemical Analysis of Tea. Shanghai Science and Technology Press, Shanghai, China (in Chinese).
  Google Scholar
• Ukers W.H. 1935. All About Tea. Tea and Coffee Trade Journals, New York.
  Google Scholar
• Wachira F., Tanaka J. and Takeda Y. 2001. Genetic variation and differentiation in tea (Camellia sinensis) germplasm revealed by RAPD and AFLP variation. J. Hortic. Sci. Bio. Tech. 76: 557–563.
  CAS Google Scholar
• Wachira F., Wauch R., Hachett C.A. and Powell W. 1995. Detection of genetic diversity in tea (Camellia sinensis) using RAPD markers. Genome 38: 201–210.
  CAS PubMed Google Scholar
• Wachira F.N., Powell W. and Waugh R. 1997. An assessment of genetic diversity among Camellia sinensis L. (cultivated tea) and its wild relatives based on randomly amplified polymorphic DNA and organelle specific STS. Heredity 78: 603–611.
  CAS Google Scholar
• Weatherstone J. 1992. Historical introduction. In: Willson K.C. and Clifford M.N. (eds), Tea: Cultivation to Consumption, Chapman and Hall, London.
  Google Scholar
• Weeden N.F. and Wendel J.F. 1989. Genetics of plant isozymes. In: Soltis D.E. and Soltis P.S. (eds), Isozymes in Plant Biology, Dioscorides Press, Portland, OR, pp. 46–72.
  Google Scholar
• Wichremaratne M.R. 1981. Variation in some leaf characteristics in tea (Camellia sinensis L.) and their use in the identification of clones. Tea Q. 50: 183–189.
  Google Scholar
• Wright S. 1978. Evolution and the Genetics of Populations, vol. 4, Variability Within and Among Natural Populations. University of Chicago Press, Chicago, IL.
  Google Scholar
• Wright W. and Gilchrist R.C.J.H. 1961. The concept of kind of tea. Nature 191: 14–16.
  Google Scholar
• Xiang D. 1962. Man Shu Jiao Zhu. Zhong Hua Shu Ju, Beijing, China (in Chinese).
  Google Scholar
• Yunnan Statistic Bureau, 2000. Yunnan Annual Statistic Report. China Statistic Press, Beijing, China, 222 pp. (in Chinese).
  Google Scholar
• Zeng Y.W., Wang J.J., Yang Z.Y., Shen S.Q., Wu L.H., Chen X.Y. and Meng J.G. 2001. The diversity and sustainable development of crop genetic resources in the Lancang River Valley. Genet. Resour. Crop Evol. 48: 297–306.
  Google Scholar

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Authors and Affiliations

1. Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, 666303, Mengla, Yunnan province, China
   Jin Chen & Yongmei Xia
2. Kunming Institute of Botany, Chinese Academy of Sciences, 650204, Kunming, Yunnan province, China
   Jin Chen & Shengji Pei
3. Tea Research Institute, Yunnan Academy of Agricultural Sciences, 666201, Menghai, Yunnan province, China
   Pingsheng Wang & Mei Xu

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1. Jin Chen
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2. Pingsheng Wang
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3. Yongmei Xia
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4. Mei Xu
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5. Shengji Pei
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Chen, J., Wang, P., Xia, Y. et al. Genetic diversity and differentiation of Camellia sinensis L. (cultivated tea) and its wild relatives in Yunnan province of China, revealed by morphology, biochemistry and allozyme studies.Genet Resour Crop Evol 52, 41–52 (2005). https://doi.org/10.1007/s10722-005-0285-1

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• Received: 12 June 2002
• Accepted: 13 June 2003
• Issue Date: February 2005
• DOI: https://doi.org/10.1007/s10722-005-0285-1

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