Biosynthesis of selenium nanoparticles by Pantoea agglomerans and their antioxidant activity (original) (raw)

Abstract

The bio-reduction of selenite (Se (IV)) generates nanoparticles with sizes ranging between 30 and 300 nm. Biologic properties of Se nanoparticles, e.g., antioxidant activity, are dependent on the nanoparticle size; smaller particles have greater activity. In this study, the bio-reduction of selenite by Pantoea agglomerans strain UC-32 under aerobic conditions and room temperature to produce bioactive Se nanoparticles smaller than 100 nm was demonstrated. Isolation and purification of the nanoparticles was performed by alkaline lysis. These purified nanoparticles were stabilized with l-cysteine (4 mM). The visualization and characterization of nanoparticles were performed by transmission electron microscopy, energy dispersive X-ray spectroscopy, and scanning electron microscopy. The antioxidant activity of nanoparticles was determined by production of reactive oxygen species using human umbilical vein endothelial cells. Transmission electron microscopy images showed the accumulation of spherical selenium nanoparticles as intracellular and extracellular deposits. The size of Se nanoparticles varied with incubation time. Amorphous Se nanoparticles with size in the order of 100 nm were obtained before 24 h of incubation; but, at 24 h of incubation, the size of the majority of the nanoparticles was in the desirable order of 100 nm and they were not aggregated. Energy dispersive spectroscopy spectra indicated that nanoparticles were composed entirely of selenium. Antioxidant activity of stabilized selenium nanoparticles demonstrated high antioxidant activity when compared to selenite and selenium nanoparticles without stabilization. Stabilized biologically synthetized selenium (0) nanoparticles with size less than 100 nm have a potential application as a food additive with antioxidant properties relevant to human health.

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References

• Belzile N, Wu J, Chen Y, Appanna VD (2006) Detoxification of selenite and mercury by reduction and mutual protection in the assimilation of both elements by Pseudomonas fluorescens. Sci Total Environ 367:704–714
  Google Scholar
• Burriel F, Lucena F, Arribas S, Hernández J (1994) Química analítica cualitativa. Paraninfo, Madrid
  Google Scholar
• Casanello P, Krause B, Torres E, Gallardo V, González M, Prieto C, Escudero C, Farías M, Sobrevia L (2009) Reduced l-arginine transport and nitric oxide synthesis in human umbilical vein endothelial cells from intrauterine growth restriction pregnancies is not further altered by hypoxia. Placenta 30:625–633
  Article CAS Google Scholar
• Chen Z, Shen Y, Xie A, Zhu J, Wu Z, Huang F (2009) l-Cysteine-assisted controlled synthesis of selenium nanospheres and nanorods. Cryst Growth Des 9:1327–1333
  Article CAS Google Scholar
• Dhanjal S, Cameotra S (2010) Aerobic biogenesis of selenium nanospheres by Bacillus cereus isolated from coalmine soil. Microb Cell Fact 9:1–11
  Article Google Scholar
• Escalante G, Campos VL, Valenzuela C, Yañez J, Zaror C, Mondaca MA (2009) Arsenic resistant bacteria isolated from arsenic contaminated river in the Atacama Desert (Chile). Bull Environ Contam Toxicol 83:657–661
  Google Scholar
• Fesharaki P, Nazari P, Shakibaie M, Rezaie S, Banoee M, Abdollahi M, Reza A (2009) Biosynthesis of selenium nanoparticles using Klebsiella pneumoniae and their recovery by a simple sterilization process. Braz J Microbiol 41:461–466
  Article Google Scholar
• Gao X, Zhang J, Zhang L (2002) Hollow sphere selenium nanoparticles. Their in vitro anti-hydroxyl radical effect. Adv Mater 14:290–293
  Article CAS Google Scholar
• Garbisu C, Ishii-Terrance T, Buchanan B (1996) Bacterial reduction of selenite to elemental selenium. Chem Geol 132:199–204
  Article CAS Google Scholar
• González M, Flores C, Pearson JD, Casanello P, Sobrevia L (2004) Cell signalling-mediating insulin increase of mRNA expression for cationic amino acid transporters 1 and 2, and membrane hyperpolarization in human umbilical vein endothelium. Pflugers Arch Eur J Physiol 448:383–394
  Google Scholar
• Hapuarachchi S, Swearingen J, Chasteen T (2004) Determination of elemental and precipitated selenium production by a facultative anaerobe grown under sequential anaerobic/aerobic conditions. Process Biochem 39:1607–1613
  Article CAS Google Scholar
• Huang B, Zhang J, Hou J, Chen C (2003) Free radical scavenging efficiency of Nano-Se in vitro. Free Radic Bio Med 35:805–813
  Article CAS Google Scholar
• Klonowska A, Heulin T, Vermeglio A (2005) Selenite and Tellurite Reduction by Shewanella oneidensis. Appl Environ Microbiol 71:5607–5609
  Article CAS Google Scholar
• Li Q, Chen T, Yang F, Liu J, Zheng W (2010) Facile and controllable one-step fabrication of selenium nanoparticles assisted by l-cysteine. Mater Lett 64:614–617
  Article CAS Google Scholar
• Li Y, Li X, Wong Y, Chen T, Zhang H, Liu C, Zheng W (2011) The reversal of cisplatin-induced nephrotoxicity by selenium nanoparticles functionalized with 11-mercapto-1-undecanol by inhibition of ROS-mediated apoptosis. Biomaterials 32:9068–9076
  Article CAS Google Scholar
• Mishra B, Priyadarsini K, Mohan H (2005) Formation of redox active nanoselenium on reactions of oxiding free radicals with selenourea. Found Day 273:262–267
  Google Scholar
• Mohampuria P, Rana N, Kumar Y (2008) Biosynthesis of nanoparticles: technological concepts and future applications. J Nanopart Res 10:507–517
  Article Google Scholar
• Okuno T, Kubota T, Ueno H, Nakamuro K (2001) Contribution of enzymic [alpha], [gamma]-elimination reaction in detoxification pathway of selenomethionine in mouse liver. Toxicol Appl Pharmacol 176:18–23
  Article CAS Google Scholar
• Oremland RS, Herbel MJ, Switzer-Blum J, Langley S, Beveridge TJ, Ajayan PM, Sutto T, Ellis AV, Curran S (2004) Structural and spectral features of selenium nanospheres produced by Se-respiring bacteria. Appl Environ Microbiol 70:52–60
  Article CAS Google Scholar
• Peng D, Zhang J, Liu Q, Taylor EW (2007) Size effect of elemental selenium nanoparticles (Nano-Se) at supranutritional levels on selenium accumulation and glutathione S-transferase activity. J Inorg Biochem 101:1457–1463
  Article CAS Google Scholar
• Prakash N, Sharma N, Prakash R, Raina K, Fellowes J, Pearce C, Lloyd J, Pattrick R (2009) Aerobic microbial manufacture of nanoscale selenium: exploiting nature’s bio-nanomineralization potential. Biotechnol Lett 31:1857–1862
  Article CAS Google Scholar
• Tam K, Ho C, Lee J, Min-Lai C, Rheem Y, Chen W, Myung NV (2010) Growth Mechanism of Amorphous Selenium Nanoparticles Synthesized by Shewanella sp. HN-41. Biosci Biotechnol Biochem 74:696–700
  Article CAS Google Scholar
• Thakkar KN, Mhatre SS, Parikh RY (2009) Biological synthesis of metallic nanoparticles. Nanomedicine: NBM 6:257–262
  Article Google Scholar
• Wang H, Zhang J, Yu H (2007) Elemental selenium at nano size possesses lower toxicity without compromising the fundamental effect on selenoenzymes: comparison with selenomethionine in mice. Free Radic Biol Med 42:1524–1533
  Article CAS Google Scholar
• Wang T, Yang L, Zhang B, Liu J (2010) Extracellular biosynthesis and transformation of selenium nanoparticles and application in H2O2 biosensor. Colloids Surf B 80:94–102
  Article CAS Google Scholar
• Xue Y, Zhao H, Wu Z, Li X, He Y, Yuan Z (2011) Colorimetric detection of Cd2 + using gold nanoparticles cofunctionalized with 6-mercaptonicotinic acid and l-cysteine. Analyst 136:3725–3730
  Article CAS Google Scholar
• Yadav V, Sharma N, Prakash R, Raina K, Bharadwaj LM, Prakas N (2008) Generation of selenium containing nano-structures by soil bacterium, Pseudomonas aeruginosa. Biotechnol 7:299–304
  Article CAS Google Scholar
• Ye G, Metreveli N, Ren J, Epstein N (2003) Metallothionein prevents diabetes-induced deficits in cardiomyocytes by inhibiting reactive oxygen species production. Diabetes 52:777–783
  Article CAS Google Scholar
• Zhang J, Wang W, Yongping B, Zhang L (2004a) Nano red elemental selenium has no size effect in the induction of seleno-enzymes in both cultured cells and mice. Life Sci 75:237–244
  Article CAS Google Scholar
• Zhang Y, Zhang J, Wang HY, Chen HY (2004b) Synthesis of selenium nanoparticles in the presence of polysaccharides. Mater Lett 58:2590–2594
  Article CAS Google Scholar
• Zhang J, Wang H, Yan X, Zhang L (2005) Comparison of short-term toxicity between Nano-Se and selenite in mice. Life Sci 76:1099–1109
  Article CAS Google Scholar
• Zhang W, Chena Z, Liua H, Zhang L, Gaoa P, Daping L (2011) Biosynthesis and structural characteristics of selenium nanoparticles by Pseudomonas alcaliphila. Colloids Surf B 88:196–201
  Article CAS Google Scholar

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Acknowledgments

The authors acknowledge the assistance of the staff of Electron Microscopy Laboratory of the University of Concepción, Chile. This work was supported by DIUC- 208.036.035-1.0 (Chile), Proyecto Basal Conicyt-Regional R08C1002 Chile), and FONDECYT 11100192 (Chile).

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Authors and Affiliations

1. Laboratorio de Microbiología Ambiental, Departamento de Microbiología, Universidad de Concepción, P.O. Box 160-C, Correo 3, Concepción, Chile
   S. K. Torres, V. L. Campos, C. G. León & M. A. Mondaca
2. Laboratorio de Fisiología Vascular, Departamento de Fisiología, Universidad de Concepción, P.O. Box 160-C, Correo 3, Concepción, Chile
   S. M. Rojas & M. González
3. Centro de Investigación de Polímeros Avanzados (CIPA), Conicyt-Regional R08C1002 Cordillera Av. 2634, Parque Industrial Coronel, Coronel, P.O. Box 4051, Post 3, Concepción, Chile
   S. M. Rodríguez-Llamazares
4. Departamento de Microbiología, Universidad de Concepción, P.O. Box 160-C, Correo 3, Concepción, Chile
   C. Smith

Authors

1. S. K. Torres
2. V. L. Campos
3. C. G. León
4. S. M. Rodríguez-Llamazares
5. S. M. Rojas
6. M. González
7. C. Smith
8. M. A. Mondaca

Corresponding author

Correspondence toV. L. Campos.

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Torres, S.K., Campos, V.L., León, C.G. et al. Biosynthesis of selenium nanoparticles by Pantoea agglomerans and their antioxidant activity.J Nanopart Res 14, 1236 (2012). https://doi.org/10.1007/s11051-012-1236-3

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• Received: 09 April 2012
• Accepted: 06 October 2012
• Published: 20 October 2012
• DOI: https://doi.org/10.1007/s11051-012-1236-3

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