The effect of Xuefu Zhuyu Decoction (血府逐瘀汤) on in vitro endothelial progenitor cell tube formation (original) (raw)

Abstract

Objective

To observe the effect of Xuefu Zhuyu Decoction (血府逐瘀汤)-containing serum (XFZYD-CS) on endothelial progenitor cell (EPC) tube formation in vitro.

Methods

Mononuclear cells from rat bone marrow were prepared in a Ficoll density gradient centrifuge. EPCs were separated by the differential attachment method, and observed with inverted microscope for the effect of XFZYD-CS on EPC tube formation.

Results

After one day, EPCs exposed to the serum containing 5%, 10% and 15% XFZYD-CS formed typical tubes or vessel networks. The tube formation time was two days ahead of the control group and the size of most tubes in the serum groups was smaller than in the control group.

Conclusion

XFZYD-CS could induce EPC angiogenesis and hasten tube formation, especially in capillary vessels. The study provides experimental evidence for the plausibility of Xuefu Zhuyu Decoction in the treatment of ischemic diseases.

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Authors and Affiliations

  1. Department of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, 350108, China
    Dong Gao (高 冬), Li-ya Wu (吴立娅), Yu-huan Jiao (焦雨欢) & Wen-yuan Chen (陈文元)
  2. Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing, 100700, China
    Yan Chen (陈 岩), Bo Lu (逯 波) & Jun Song (宋 军)
  3. Harvard Medical School, Boston, MA, 02215, USA
    Ted J. Kaptchuk
  4. Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, 100091, China
    Ke-ji Chen (陈可冀)

Authors

  1. Dong Gao (高 冬)
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  2. Li-ya Wu (吴立娅)
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  3. Yu-huan Jiao (焦雨欢)
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  4. Wen-yuan Chen (陈文元)
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  5. Yan Chen (陈 岩)
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  6. Ted J. Kaptchuk
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  7. Bo Lu (逯 波)
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  8. Jun Song (宋 军)
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  9. Ke-ji Chen (陈可冀)
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Corresponding author

Correspondence toJun Song (宋 军).

Additional information

Supported by the National Natural Science Foundation of China (No. 30772877); Basic Research Fund of China Academy of Chinese Medical Sciences (No. ZZ2006039); Fujian Academy of Integrative Medicine Foundation (No. 3000-905010805)

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Gao, D., Wu, Ly., Jiao, Yh. et al. The effect of Xuefu Zhuyu Decoction (血府逐瘀汤) on in vitro endothelial progenitor cell tube formation.Chin. J. Integr. Med. 16, 50–53 (2010). https://doi.org/10.1007/s11655-010-0050-y

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