Biological synthesis and characterization of intracellular gold nanoparticles using biomass of Aspergillus fumigatus (original) (raw)

Abstract

Nanotechnology is emerging as one of the most important and revolutionizing area in research field. Nanoparticles are produced by various methods like physical, chemical, mechanical and biological. Biological methods of reduction of metal ions using plants or microorganisms are often preferred because they are clean, non-toxic, safe, biocompatible and environmentally acceptable. In the present study, Aspergillus fumigatus was used for the intracellular synthesis of gold nanoparticles. Stable nanoparticles were produced when an aqueous solution of chloroauric acid (HAuCl4) was reduced by A. fumigatus biomass as the reducing agent. Production of nanoparticles was confirmed by the colour change from yellow to pinkish violet after ∼ 72 h of reaction. The produced nanoparticles were then characterized by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM), energy dispersive spectroscopy (EDS) and X-ray diffraction spectroscopy (XRD). SEM images of sample revealed that the nanoparticles were spherical, irregularly shaped with indefinite morphology. Biosynthesized gold nanoparticles were in the range of 85·1–210 nm in size. The presence of gold nanoparticle was confirmed by EDS analysis. Crystalline nature and face-centred cubic structure of synthesized gold nanoparticle was confirmed by XRD pattern.

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Acknowledgments

The authors would like to thank SRM University, Chennai for their SEM–EDS, XRD and FT–IR analyses.

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Authors and Affiliations

1. Department of Biotechnology, St. Joseph’s College of Engineering, Chennai, 600 119, India
   PRANAV VASANTHI BATHRINARAYANAN, DILLIGANESH THANGAVELU, VASANTH KUMAR MUTHUKUMARASAMY, CHAMUNDEESWARI MUNUSAMY & BASKAR GURUNATHAN

Authors

1. PRANAV VASANTHI BATHRINARAYANAN
2. DILLIGANESH THANGAVELU
3. VASANTH KUMAR MUTHUKUMARASAMY
4. CHAMUNDEESWARI MUNUSAMY
5. BASKAR GURUNATHAN

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Correspondence toBASKAR GURUNATHAN.

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VASANTHI BATHRINARAYANAN, P., THANGAVELU, D., MUTHUKUMARASAMY, V.K. et al. Biological synthesis and characterization of intracellular gold nanoparticles using biomass of Aspergillus fumigatus .Bull Mater Sci 36, 1201–1205 (2013). https://doi.org/10.1007/s12034-013-0599-0

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• Received: 18 July 2012
• Revised: 01 September 2012
• Published: 10 April 2014
• Issue date: December 2013
• DOI: https://doi.org/10.1007/s12034-013-0599-0

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