Mammalian Cell Culture Types and Guidelines of Their Maintenance (original) (raw)

Abstract

Mammalian cell culture can be classified into three types, namely, primary, secondary, and continuous cultures. The maintenance and growth of cells isolated directly from the parental mammalian tissues/organs in cultures comprise the primary cell cultures. When the primary cultured cells are further subcultured (propagated) or passaged in new culture vessels, it is called secondary culture. Since secondary cultured cells are derived from the primary cultured cells, they are also called extensions or continuations of primary culture. The normal mammalian cells have a finite ability to undergo divisions in physiological conditions, and that is why these cells are mortal. So, primary and secondary cultured cells originate from the normal mortal cells having a capacity of limited divisions. However, in certain pathophysiological conditions such as cancer, few cells can be originated within a mammalian body with a capacity to divide uninterruptedly or continuously as long as they are supplied with proper nutrition and an ideal growth environment. Because of the continuous or infinite division capacity, this type of cell culture is also called continuous cell culture. Additionally, mammalian cells possess a slightly negative charge on their plasma membrane and require adherence with the cultured vessels for their growth or proliferation. While most mammalian cells are adherent in nature, some blood cells (e.g., B/T lymphocytes) are nonadherent. This chapter narrates primary, secondary, and continuous cell culture, as well as adherent and nonadherent cell culture procedures.

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Authors and Affiliations

1. School of Chemical Sciences, Central University of Gujarat, Gandhinagar, India
   Parth Malik
2. Department of Immunopathology, Research Block A, Post Graduate Institute of Medical Education and Research (PGIMER), Chandigarh, India
   Srirupa Mukherjee
3. Amity University, Noida, Uttar Pradesh, India
   Tapan Kumar Mukherjee

Authors

1. Parth Malik
2. Srirupa Mukherjee
3. Tapan Kumar Mukherjee

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Editors and Affiliations

1. Amity University, Noida, Uttar Pradesh, India
   Tapan Kumar Mukherjee
2. School of Nano Sciences, Central University of Gujarat, Gandhinagar, Gujarat, India
   Parth Malik
3. Department of Immunopathology, Post Graduate Institute of Medical Education, Chandigarh, Chandigarh, India
   Srirupa Mukherjee

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Malik, P., Mukherjee, S., Mukherjee, T.K. (2023). Mammalian Cell Culture Types and Guidelines of Their Maintenance. In: Mukherjee, T.K., Malik, P., Mukherjee, S. (eds) Practical Approach to Mammalian Cell and Organ Culture. Springer, Singapore. https://doi.org/10.1007/978-981-19-1731-8\_6-2

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• DOI: https://doi.org/10.1007/978-981-19-1731-8\_6-2
• Received: 15 March 2022
• Accepted: 15 March 2022
• Published: 20 January 2023
• Publisher Name: Springer, Singapore
• Print ISBN: 978-981-19-1730-1
• Online ISBN: 978-981-19-1731-8
• eBook Packages: Biomedical and Life SciencesReference Module Biomedical and Life Sciences

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1. Latest

Mammalian Cell Culture Types and Guidelines of Their Maintenance
Published:
20 January 2023
DOI: https://doi.org/10.1007/978-981-19-1731-8\_6-2 2. #### Original
Mammalian Cell Culture Types and Guidelines for Their Maintenance
Published:
22 December 2022
DOI: https://doi.org/10.1007/978-981-19-1731-8\_6-1