Carla Rosa | Universidade de Lisboa (original) (raw)

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Papers by Carla Rosa

Research paper thumbnail of Identification of Flatfish ( Pleuronectiforme ) Species Using DNA-Based Techniques

Journal of Agricultural and Food Chemistry, 2001

Research paper thumbnail of Identification of Hake Species ( Merluccius Genus) Using Sequencing and PCR−RFLP Analysis of Mitochondrial DNA Control Region Sequences

Journal of Agricultural and Food Chemistry, 2001

Research paper thumbnail of Validation of a PCR-RFLP based method for the identification of salmon species in food products

European Food Research and Technology, 2001

The use of a PCR-RFLP based method for the identification of salmon species in food products was... more The use of a PCR-RFLP based method for the identification of salmon species in food products was investigated. The reliability and practicality of the method was tested by a collaborative study in which five European laboratories participated. Two unknown samples (a commercial product of known species composition and a mix of two salmon species) required identification by comparison with authentic reference species. From a total of 50 cases, 100% of authentic species were correctly assigned, with all unknown samples also correctly identified. A larger scale analysis of UK commercial products was also performed spanning the whole range of salmon products available. In almost all cases the salmon species declared was confirmed, although, a trout species was detected in one product declaring only the presence of salmon. The investigation confirms the reproducibility of the method in different laboratories, and its applicability for commercial product analysis.

Research paper thumbnail of Development of a DNA-Based Method Aimed at Identifying the Fish Species Present in Food Products

Journal of Agricultural and Food Chemistry, 2001

Research paper thumbnail of Use of Restriction Fragment Length Polymorphism To Distinguish between Salmon Species

Journal of Agricultural and Food Chemistry, 2000

Research paper thumbnail of Identification of gadoid fish species using DNA-based techniques

European Food Research and Technology, 2003

The polymerase chain reaction (PCR) technique was employed to obtain a 464 bp amplicon from the m... more The polymerase chain reaction (PCR) technique was employed to obtain a 464 bp amplicon from the mitochondrial cytochrome b gene from gadoid species to study its ability to differentiate them. The sequences of this fragment from 16 species were analysed using a genetic distance method, and polymorphic sites were determined. The fragment was shown to be moderately polymorphic (151 sites), and this permitted the differentiation of most of the species. A phylogenetic tree construction using Tamura-Nei distances was employed to allow the identification of Gadidae species, each species resulted in a well-differentiated clade, with the exception of Gadus ogac and Gadus macrocephalus, which could not be differentiated. Based on the sequences obtained, three restriction enzymes, Dde I, Hinc II and Nla III, were selected to provide specific restriction profiles, which allowed the differentiation of 15 species of gadoids in a faster and less expensive way than sequencing. The PCR-restriction fragment length polymorphism methodology was also tested using commercial samples.

Research paper thumbnail of Differentiation of raw or processed eel by PCR-based techniques: restriction fragment length polymorphism analysis (RFLP) and single strand conformation polymorphism analysis (SSCP

European Food Research and Technology, 2002

PCR-based methods have been developed to differentiate four species of commercially important eel... more PCR-based methods have been developed to differentiate four species of commercially important eels (Anguilla anguilla, A. rostrata, A. japonica, A. australis). By means of universal primers a 464 base pair (bp) section of the cytochrome b gene was amplified. The amplicon was sequenced and restriction enzymes were selected for RFLP analysis; a short part (123 bp) of the 464 bp section was amplified by another set of primers and used for SSCP analysis. The suitability of RFLP and SSCP for differentiating hot-smoked eel was verified by a collaborative study.

Research paper thumbnail of Identification of Cephalopod Species (Ommastrephidae and Loliginidae) in Seafood Products by Forensically Informative Nucleotide Sequencing (FINS

Journal of Food Science, 2002

ABSTRACT: An identification technique of commercially important cephalopods based on 16s RNA anal... more ABSTRACT: An identification technique of commercially important cephalopods based on 16s RNA analysis was developed. A set of primers was designed to amplify a fragment of approximately 200 bp that presents enough variability for reliable species identification. Sequences from this fragment of 9 different authentic species were studied, genetic distances were measured, and a phylogenetic tree was constructed, with individuals of the same species grouped within the same cluster. Eight different types of commercial seafood products, mainly labelled as “squid rings”, were analyzed and sequences were employed for species identification showing that FINS is a suitable technique for identification of processed cephalopods.

Research paper thumbnail of Identification of Flatfish ( Pleuronectiforme ) Species Using DNA-Based Techniques

Journal of Agricultural and Food Chemistry, 2001

Research paper thumbnail of Identification of Hake Species ( Merluccius Genus) Using Sequencing and PCR−RFLP Analysis of Mitochondrial DNA Control Region Sequences

Journal of Agricultural and Food Chemistry, 2001

Research paper thumbnail of Validation of a PCR-RFLP based method for the identification of salmon species in food products

European Food Research and Technology, 2001

The use of a PCR-RFLP based method for the identification of salmon species in food products was... more The use of a PCR-RFLP based method for the identification of salmon species in food products was investigated. The reliability and practicality of the method was tested by a collaborative study in which five European laboratories participated. Two unknown samples (a commercial product of known species composition and a mix of two salmon species) required identification by comparison with authentic reference species. From a total of 50 cases, 100% of authentic species were correctly assigned, with all unknown samples also correctly identified. A larger scale analysis of UK commercial products was also performed spanning the whole range of salmon products available. In almost all cases the salmon species declared was confirmed, although, a trout species was detected in one product declaring only the presence of salmon. The investigation confirms the reproducibility of the method in different laboratories, and its applicability for commercial product analysis.

Research paper thumbnail of Development of a DNA-Based Method Aimed at Identifying the Fish Species Present in Food Products

Journal of Agricultural and Food Chemistry, 2001

Research paper thumbnail of Use of Restriction Fragment Length Polymorphism To Distinguish between Salmon Species

Journal of Agricultural and Food Chemistry, 2000

Research paper thumbnail of Identification of gadoid fish species using DNA-based techniques

European Food Research and Technology, 2003

The polymerase chain reaction (PCR) technique was employed to obtain a 464 bp amplicon from the m... more The polymerase chain reaction (PCR) technique was employed to obtain a 464 bp amplicon from the mitochondrial cytochrome b gene from gadoid species to study its ability to differentiate them. The sequences of this fragment from 16 species were analysed using a genetic distance method, and polymorphic sites were determined. The fragment was shown to be moderately polymorphic (151 sites), and this permitted the differentiation of most of the species. A phylogenetic tree construction using Tamura-Nei distances was employed to allow the identification of Gadidae species, each species resulted in a well-differentiated clade, with the exception of Gadus ogac and Gadus macrocephalus, which could not be differentiated. Based on the sequences obtained, three restriction enzymes, Dde I, Hinc II and Nla III, were selected to provide specific restriction profiles, which allowed the differentiation of 15 species of gadoids in a faster and less expensive way than sequencing. The PCR-restriction fragment length polymorphism methodology was also tested using commercial samples.

Research paper thumbnail of Differentiation of raw or processed eel by PCR-based techniques: restriction fragment length polymorphism analysis (RFLP) and single strand conformation polymorphism analysis (SSCP

European Food Research and Technology, 2002

PCR-based methods have been developed to differentiate four species of commercially important eel... more PCR-based methods have been developed to differentiate four species of commercially important eels (Anguilla anguilla, A. rostrata, A. japonica, A. australis). By means of universal primers a 464 base pair (bp) section of the cytochrome b gene was amplified. The amplicon was sequenced and restriction enzymes were selected for RFLP analysis; a short part (123 bp) of the 464 bp section was amplified by another set of primers and used for SSCP analysis. The suitability of RFLP and SSCP for differentiating hot-smoked eel was verified by a collaborative study.

Research paper thumbnail of Identification of Cephalopod Species (Ommastrephidae and Loliginidae) in Seafood Products by Forensically Informative Nucleotide Sequencing (FINS

Journal of Food Science, 2002

ABSTRACT: An identification technique of commercially important cephalopods based on 16s RNA anal... more ABSTRACT: An identification technique of commercially important cephalopods based on 16s RNA analysis was developed. A set of primers was designed to amplify a fragment of approximately 200 bp that presents enough variability for reliable species identification. Sequences from this fragment of 9 different authentic species were studied, genetic distances were measured, and a phylogenetic tree was constructed, with individuals of the same species grouped within the same cluster. Eight different types of commercial seafood products, mainly labelled as “squid rings”, were analyzed and sequences were employed for species identification showing that FINS is a suitable technique for identification of processed cephalopods.

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