Rita Serrano | Universidade de Lisboa (original) (raw)

Papers by Rita Serrano

Research paper thumbnail of Desenvolvimento de Formulações Farmacêuticas com base em Plantas Medicinais e estandardização de controlo de qualidade

Research paper thumbnail of Azolla em Portugal

Research paper thumbnail of Botanical characterization of Hypericum androsaemum a Portuguese medicinal plant

Research paper thumbnail of Terminalia genus as source of antimicrobial agents

Species of Terminalia L. genus (Combretaceae) were worldwide used in traditional medicine to trea... more Species of Terminalia L. genus (Combretaceae) were worldwide used in traditional medicine to treat different kind of diseases. In Africa, 30 Terminalia species were described and the traditional uses of most of it as antimicrobial agents were reported by different authors. In the present work a summary of the principal studies and results concerning the evaluation of the potential of 11 Terminalia species from Africa, America, Asia and Oceania as antibacterial, antiviral, antifungal and antiprotozoal agents or source of these will be presented. In detail, results obtained by our team concerning the antimicrobial activity of Terminalia macroptera Guill & Perr., a tree widely distributed in the savannahs and woodlands of Tropical Africa and used in Guinea-Bissau to treat infectious diarrhoeal diseases and venereal diseases, will be presented and discussed. Extracts of T. macroptera leaf and/or root have been found to be slight active against Candida albicans, and showing an interestin...

Research paper thumbnail of Botanical characterization of Hypericum foliosum an endemic Azorean species

Research paper thumbnail of Phenol content, antioxidant and α- and β-glucosidase inhibitory activities of an Anacardium occidentale stem bark Traditional Herbal Preparation

Planta Medica, 2014

Introduction: Anacardium occidentale L., commonly known as cashew tree, has been traditionally us... more Introduction: Anacardium occidentale L., commonly known as cashew tree, has been traditionally used in several countries of Asia, Africa and America, due to its antihyperglycemic properties. Aim of work: To perform comparative studies between Traditional Herbal Preparation (THP) of A. occidentale stem bark red (AOR) and white (AOW) in order to assess their free radical scavenging, α- and β-glucosidase inhibition activities and total phenolic content. Methods: THP total phenol content was determined by the Folin-Ciocalteu method [1], using gallic acid as standard. Simultaneously, the free radical scavenging activity of the THPs was assessed with a 1,1-diphenyl-2-picrylhydrazine (DPPH) assay, using ascorbic acid as reference standard [2]. Moreover bioautographic assays with thin layer chromatography were performed for the detection of α- and β-glucosidase inhibitors [3]. Results: Total phenolic content was found to be 51.3 ± 1.6 and 58.0 ± 0.4 mg of gallic acid equivalent per gram of dried cashew stem bark in AOR and AOW THP, respectively. Concerning the free radical scavenging activity, IC50 value was 180.7 ± 6.7 µg/mL for the AOR THP and 143.8 ± 2.8 µg/mL for the AOW THP. Bioautography showed two chromatogram zones that were identified as α-glucosidase (Rf between 0.55 and 0.68) and β-glucosidase (Rf between 0.63 and 0.87) inhibitors in both THP. Conclusion: Assessed THP proved to be a source of natural antioxidants that may scavenge free radicals. According to data obtained from all experimental assays, AOW THP has higher antioxidant activity and total phenolic content than AOR THP. Moreover, extracts from both types of A. occidentale promoted inhibition of α- and β-glucosidase. Acknowledgements: This work was supported by grant Pest-OE/SAU/UI4013/2011 from Fundacao para a Ciencia e Tecnologia, Lisbon, Portugal. Keywords: Cashew stem bark, Traditional Herbal Preparation References: [1] Scalbert A, Monties B, Jannin G. Tannins in wood: comparison of different estimation methods. J Agricult Food Chem 1989; 37(5): 1324 – 1329. [2] Silva T, Camara C, Lins A, Barbosa-Filho J, Silva E, Freitas B, Santos F. Chemical composition and free radical scavenging activity of pollen loads from stingless bee Melipona subnitida Ducke. J Food Comp Anal 2006; 19: 507 – 511. [3] Simoes-Pires C, Hmicha B, Marston A, Hostettmann K. A TLC bioautographic method for the detection of α- and β-glucosidase inhibitors in plant extracts. Phytochem Anal 2009; 20: 511 – 515.

Research paper thumbnail of Caracterização farmacognóstica da raíz de Euclea natalensis

Research paper thumbnail of Identificação botânica de Maytenus senegalensis folha como fármaco vegetal

Research paper thumbnail of A review of the role of medicinal plants on Neisseria gonorrhoeae infection

European Journal of Integrative Medicine

Research paper thumbnail of A Comprehensive Review on the Medicinal Plants from the Genus Asphodelus

Plants

Plant-based systems continue to play an essential role in healthcare, and their use by different ... more Plant-based systems continue to play an essential role in healthcare, and their use by different cultures has been extensively documented. Asphodelus L. (Asphodelaceae) is a genus of 18 species and of a total of 27 species, sub-species and varieties, distributed along the Mediterranean basin, and has been traditionally used for treating several diseases particularly associated with inflammatory and infectious skin disorders. The present study aimed to provide a general review of the available literature on ethnomedical, phytochemical, and biological data related to the genus Asphodelus as a potential source of new compounds with biological activity. Considering phytochemical studies, 1,8-dihydroxyanthracene derivatives, flavonoids, phenolic acids and triterpenoids were the main classes of compounds identified in roots, leaf and seeds which were correlated with their biological activities as anti-microbial, anti-fungal, anti-parasitic, cytotoxic, anti-inflammatory or antioxidant agents.

Research paper thumbnail of Toxic Plants: Knowledge, Medicinal Uses and Potential Human Health Risks

Environment and Ecology Research

A toxic plant is a plant that when touched or ingested in sufficient quantity can be harmful or f... more A toxic plant is a plant that when touched or ingested in sufficient quantity can be harmful or fatal to man or other animals. When used in small amounts and correct proportions, these plants can be used as herbal drugs with medicinal properties. According to official sources from several countries, injurious and poisonous plants are responsible for many cases of intoxications worldwide. The beauty of these plants hides their toxicity and the unawareness potentiates these situations. Therefore, this bibliographic revision is about examples of some plants associated with poisoning but used for their medicinal properties, focusing on species with anticholinergic poisons (Atropa belladonna L., Datura stramonium L., Hyoscyamus niger L.), with calcium oxalate crystals (Philodendron spp.), with cardiac glycosides (Digitalis purpurea L.), with convulsant poisons and with nicotine-like alkaloids (Conium maculatum L., Nicotiana glauca Graham), with mitotic inhibitors (Catharanthus roseus L., Colchicum autumnale L.), with pyrrolizidine alkaloids (Senecio vulgaris L.), with sodium channel activators (Aconitum napellus L., Rhododendron spp.), with cyanogenic compounds (Hydrangea macrophylla (Thunb.) Ser.), and with toxalbumins (Abrus precatorius L., Ricinus communis L.). The knowledge about toxic plants is still insufficient and makes it so important to characterize some species that cause intoxication and lethality in humans.

Research paper thumbnail of Comparative study on the in vivo antidepressant activities of the Portuguese Hypericum foliosum, Hypericum androsaemum and Hypericum perforatum medicinal plants

Industrial Crops and Products, 2015

Research paper thumbnail of Caracterização preliminar de camadas S presentes em estirpes de Corynebacterium e de Rhodococcus contidas na cavidade da folha de Azolla filliculoides

Research paper thumbnail of Camadas superficiais em bactérias simbiontes de Azolla filiculoides Lam

Research paper thumbnail of Contribuição para o estudo de bactérias simbiontes existentes no sistema Azolla-Anabaena

Research paper thumbnail of Azolla-Bacterial symbiosis, detection and preliminary characterization of lectins

Research paper thumbnail of Actividade hemaglutinante de extractos de simbiontes contidos na cavidade da folha de Azolla filliculoides e de Azolla pinnata

Research paper thumbnail of Carrapiço F, Antunes T, Sevinate-Pinto I., Teixeira G., Serrano R., Baioa M. V., Pereira A.L., Elias F., Bastos M., Caixinhas R., Rafael T. & Falcão M., 2001. Azolla em Portugal. Instituto da Água, CBA, FCUL, MAOT (Eds). Lisboa, 16 p. (ISBN 972-9412-58-8)

Carrapiço F, Antunes T, Sevinate-Pinto I., Teixeira G., Serrano R., Baioa M. V., Pereira A.L., Elias F., Bastos M., Caixinhas R., Rafael T. & Falcão M., 2001. Azolla em Portugal. Instituto da Água, CBA, FCUL, MAOT (Eds). Lisboa, 16 p. (ISBN 972-9412-58-8)

Research paper thumbnail of Afinidade do vermelho do Congo para estirpes de Corynebacterium e de Rhodococcus da folha de Azolla filiculoides

Research paper thumbnail of Quercus suber 23S ribosomal RNA gene, partial sequence; internal transcribed spacer 2, 4.5S ribosomal RNA gene and internal transcribed spacer 3, complete sequence; and 5S ribosomal RNA gene, partial sequence; chloroplast genes for chloroplast products

Research paper thumbnail of Desenvolvimento de Formulações Farmacêuticas com base em Plantas Medicinais e estandardização de controlo de qualidade

Research paper thumbnail of Azolla em Portugal

Research paper thumbnail of Botanical characterization of Hypericum androsaemum a Portuguese medicinal plant

Research paper thumbnail of Terminalia genus as source of antimicrobial agents

Species of Terminalia L. genus (Combretaceae) were worldwide used in traditional medicine to trea... more Species of Terminalia L. genus (Combretaceae) were worldwide used in traditional medicine to treat different kind of diseases. In Africa, 30 Terminalia species were described and the traditional uses of most of it as antimicrobial agents were reported by different authors. In the present work a summary of the principal studies and results concerning the evaluation of the potential of 11 Terminalia species from Africa, America, Asia and Oceania as antibacterial, antiviral, antifungal and antiprotozoal agents or source of these will be presented. In detail, results obtained by our team concerning the antimicrobial activity of Terminalia macroptera Guill & Perr., a tree widely distributed in the savannahs and woodlands of Tropical Africa and used in Guinea-Bissau to treat infectious diarrhoeal diseases and venereal diseases, will be presented and discussed. Extracts of T. macroptera leaf and/or root have been found to be slight active against Candida albicans, and showing an interestin...

Research paper thumbnail of Botanical characterization of Hypericum foliosum an endemic Azorean species

Research paper thumbnail of Phenol content, antioxidant and α- and β-glucosidase inhibitory activities of an Anacardium occidentale stem bark Traditional Herbal Preparation

Planta Medica, 2014

Introduction: Anacardium occidentale L., commonly known as cashew tree, has been traditionally us... more Introduction: Anacardium occidentale L., commonly known as cashew tree, has been traditionally used in several countries of Asia, Africa and America, due to its antihyperglycemic properties. Aim of work: To perform comparative studies between Traditional Herbal Preparation (THP) of A. occidentale stem bark red (AOR) and white (AOW) in order to assess their free radical scavenging, α- and β-glucosidase inhibition activities and total phenolic content. Methods: THP total phenol content was determined by the Folin-Ciocalteu method [1], using gallic acid as standard. Simultaneously, the free radical scavenging activity of the THPs was assessed with a 1,1-diphenyl-2-picrylhydrazine (DPPH) assay, using ascorbic acid as reference standard [2]. Moreover bioautographic assays with thin layer chromatography were performed for the detection of α- and β-glucosidase inhibitors [3]. Results: Total phenolic content was found to be 51.3 ± 1.6 and 58.0 ± 0.4 mg of gallic acid equivalent per gram of dried cashew stem bark in AOR and AOW THP, respectively. Concerning the free radical scavenging activity, IC50 value was 180.7 ± 6.7 µg/mL for the AOR THP and 143.8 ± 2.8 µg/mL for the AOW THP. Bioautography showed two chromatogram zones that were identified as α-glucosidase (Rf between 0.55 and 0.68) and β-glucosidase (Rf between 0.63 and 0.87) inhibitors in both THP. Conclusion: Assessed THP proved to be a source of natural antioxidants that may scavenge free radicals. According to data obtained from all experimental assays, AOW THP has higher antioxidant activity and total phenolic content than AOR THP. Moreover, extracts from both types of A. occidentale promoted inhibition of α- and β-glucosidase. Acknowledgements: This work was supported by grant Pest-OE/SAU/UI4013/2011 from Fundacao para a Ciencia e Tecnologia, Lisbon, Portugal. Keywords: Cashew stem bark, Traditional Herbal Preparation References: [1] Scalbert A, Monties B, Jannin G. Tannins in wood: comparison of different estimation methods. J Agricult Food Chem 1989; 37(5): 1324 – 1329. [2] Silva T, Camara C, Lins A, Barbosa-Filho J, Silva E, Freitas B, Santos F. Chemical composition and free radical scavenging activity of pollen loads from stingless bee Melipona subnitida Ducke. J Food Comp Anal 2006; 19: 507 – 511. [3] Simoes-Pires C, Hmicha B, Marston A, Hostettmann K. A TLC bioautographic method for the detection of α- and β-glucosidase inhibitors in plant extracts. Phytochem Anal 2009; 20: 511 – 515.

Research paper thumbnail of Caracterização farmacognóstica da raíz de Euclea natalensis

Research paper thumbnail of Identificação botânica de Maytenus senegalensis folha como fármaco vegetal

Research paper thumbnail of A review of the role of medicinal plants on Neisseria gonorrhoeae infection

European Journal of Integrative Medicine

Research paper thumbnail of A Comprehensive Review on the Medicinal Plants from the Genus Asphodelus

Plants

Plant-based systems continue to play an essential role in healthcare, and their use by different ... more Plant-based systems continue to play an essential role in healthcare, and their use by different cultures has been extensively documented. Asphodelus L. (Asphodelaceae) is a genus of 18 species and of a total of 27 species, sub-species and varieties, distributed along the Mediterranean basin, and has been traditionally used for treating several diseases particularly associated with inflammatory and infectious skin disorders. The present study aimed to provide a general review of the available literature on ethnomedical, phytochemical, and biological data related to the genus Asphodelus as a potential source of new compounds with biological activity. Considering phytochemical studies, 1,8-dihydroxyanthracene derivatives, flavonoids, phenolic acids and triterpenoids were the main classes of compounds identified in roots, leaf and seeds which were correlated with their biological activities as anti-microbial, anti-fungal, anti-parasitic, cytotoxic, anti-inflammatory or antioxidant agents.

Research paper thumbnail of Toxic Plants: Knowledge, Medicinal Uses and Potential Human Health Risks

Environment and Ecology Research

A toxic plant is a plant that when touched or ingested in sufficient quantity can be harmful or f... more A toxic plant is a plant that when touched or ingested in sufficient quantity can be harmful or fatal to man or other animals. When used in small amounts and correct proportions, these plants can be used as herbal drugs with medicinal properties. According to official sources from several countries, injurious and poisonous plants are responsible for many cases of intoxications worldwide. The beauty of these plants hides their toxicity and the unawareness potentiates these situations. Therefore, this bibliographic revision is about examples of some plants associated with poisoning but used for their medicinal properties, focusing on species with anticholinergic poisons (Atropa belladonna L., Datura stramonium L., Hyoscyamus niger L.), with calcium oxalate crystals (Philodendron spp.), with cardiac glycosides (Digitalis purpurea L.), with convulsant poisons and with nicotine-like alkaloids (Conium maculatum L., Nicotiana glauca Graham), with mitotic inhibitors (Catharanthus roseus L., Colchicum autumnale L.), with pyrrolizidine alkaloids (Senecio vulgaris L.), with sodium channel activators (Aconitum napellus L., Rhododendron spp.), with cyanogenic compounds (Hydrangea macrophylla (Thunb.) Ser.), and with toxalbumins (Abrus precatorius L., Ricinus communis L.). The knowledge about toxic plants is still insufficient and makes it so important to characterize some species that cause intoxication and lethality in humans.

Research paper thumbnail of Comparative study on the in vivo antidepressant activities of the Portuguese Hypericum foliosum, Hypericum androsaemum and Hypericum perforatum medicinal plants

Industrial Crops and Products, 2015

Research paper thumbnail of Caracterização preliminar de camadas S presentes em estirpes de Corynebacterium e de Rhodococcus contidas na cavidade da folha de Azolla filliculoides

Research paper thumbnail of Camadas superficiais em bactérias simbiontes de Azolla filiculoides Lam

Research paper thumbnail of Contribuição para o estudo de bactérias simbiontes existentes no sistema Azolla-Anabaena

Research paper thumbnail of Azolla-Bacterial symbiosis, detection and preliminary characterization of lectins

Research paper thumbnail of Actividade hemaglutinante de extractos de simbiontes contidos na cavidade da folha de Azolla filliculoides e de Azolla pinnata

Research paper thumbnail of Carrapiço F, Antunes T, Sevinate-Pinto I., Teixeira G., Serrano R., Baioa M. V., Pereira A.L., Elias F., Bastos M., Caixinhas R., Rafael T. & Falcão M., 2001. Azolla em Portugal. Instituto da Água, CBA, FCUL, MAOT (Eds). Lisboa, 16 p. (ISBN 972-9412-58-8)

Carrapiço F, Antunes T, Sevinate-Pinto I., Teixeira G., Serrano R., Baioa M. V., Pereira A.L., Elias F., Bastos M., Caixinhas R., Rafael T. & Falcão M., 2001. Azolla em Portugal. Instituto da Água, CBA, FCUL, MAOT (Eds). Lisboa, 16 p. (ISBN 972-9412-58-8)

Research paper thumbnail of Afinidade do vermelho do Congo para estirpes de Corynebacterium e de Rhodococcus da folha de Azolla filiculoides

Research paper thumbnail of Quercus suber 23S ribosomal RNA gene, partial sequence; internal transcribed spacer 2, 4.5S ribosomal RNA gene and internal transcribed spacer 3, complete sequence; and 5S ribosomal RNA gene, partial sequence; chloroplast genes for chloroplast products