Devendra Kumar pandey | LOVELY PROFESSIONAL UNIVERSITY (original) (raw)
Drafts by Devendra Kumar pandey
Reserach Area I have carried out extensive research in the area of authentication and standardiza... more Reserach Area
I have carried out extensive research in the area of authentication and standardization of Medicinal and Aromatic plants by phytochemical and molecular techniques. I am well versed in chromatographic techniques i.e High performance thin layer chromatograph, High pressure liquid chromatography, Gas Chromatography as well as DNA based molecular marker technology. I have worked on RAPD, AFLP, ISSR and STMS markers for determination of genetic variability and identification of superior germplam of MAPs i.e Aloe vera, Gloriosa superba, Lantana camara, Ocimum sanctum, Bacopa monnieri , Rauwolfia serpentine, Stevia rebaudiana and Swertia chirata. In addition to routine testing of using Phytochemical and PCR based markers, I have standardized micropropagation protocols of various medicinal and aromatic plants (MAPs) such as Swertia chirata, Aloe vera, Stevia rebaudiana, Rauwolfia serpentine, Bacopa monnierie, Hemidesmus indicus,Dioscorea deltoidea, Bacopa monnierie, Aristolochia indica.
Papers by Devendra Kumar pandey
BMC Complementary Medicine and Therapies, 2020
Background Mahasudarshan Churna (MC) is a polyherbal Ayurvedic medicine that is employed in fever... more Background Mahasudarshan Churna (MC) is a polyherbal Ayurvedic medicine that is employed in fever (especially chronic type), cold and malaria, improvement of digestion and appetite, removes toxins from the blood, boosts immunity and protects against common bacterial infections. Methods Validation and quantification of oleanolic acid (OA), ursolic acid (UA), mangiferin (M), gallic acid (GA), quercetin (Q) and curcumin (C) in commercial MC formulations by HPTLC method. Mobile phase, hexane: ethyl acetate: acetone (16.4: 3.6: 0.2, v/v) was used for the separation of OA and UA; ethyl acetate: glacial acetic acid: formic acid: water (20: 2.2: 2.2: 5.2 v/v) for the development of M; and toluene: ethyl acetate: formic acid (13.5: 9: 0.6 v/v) for the separation of GA, Q and C in crude sample extracts. Visualization and scanning were performed at λ = 530 nm for OA and UA, at λ = 254 nm for M and at λ = 366 nm for GA, Q and C. In addition, HPLC-PDA analysis was used to confirm the HPTLC resul...
Scientific Reports, 2021
Swertia chirata Buch.-Ham. ex C.B. Clarke is an important medicinal plant used in various herbal ... more Swertia chirata Buch.-Ham. ex C.B. Clarke is an important medicinal plant used in various herbal formulations as it shows significant biological activities such as hepatoprotective, hypoglycemic, anti-inflammatory, antimalarial, antioxidant and anti-parkinson. C-glucosyl xanthone glycoside (mangiferin) is known as bio-marker compound of genus Swertia L. Development of efficient extraction methods of C-glucosyl xanthone mangiferin from Swertia chirata was attempted by optimizing the pre-harvest, post-harvest and extraction techniques by full factorial design. Firstly, a full factorial design was implemented to evaluate the single and interactive effects of pre-harvest (growth stage and plant part), post-harvest (drying condition and storage periods) followed by selection of best extraction technique such as heat reflux extraction (HRE), microwave assisted extraction (MAE) and ultrasound assistant extraction (UAE) at different solvent types on mangiferin yield. HPTLC and HPLC techniqu...
BMC Plant Biology, 2020
Background In this study, response surface methodology (RSM) and artificial neural network (ANN) ... more Background In this study, response surface methodology (RSM) and artificial neural network (ANN) was used to construct the predicted models of linear, quadratic and interactive effects of two independent variables viz. salicylic acid (SA) and chitosan (CS) for the production of amarogentin (I), swertiamarin (II) and mangiferin (III) from shoot cultures of Swertia paniculata Wall. These compounds are the major therapeutic metabolites in the Swertia plant, which have significant role and demand in the pharmaceutical industries. Results Present study highlighted that different concentrations of SA and CS elicitors substantially influenced the % yield of (I), (II) and (III) compounds in the shoot culture established on modified ½ MS medium (supplemented with 2.22 mM each of BA and KN and 2.54 mM NAA). In RSM, different response variables with linear, quadratic and 2 way interaction model were computed with five-factor-three level full factorial CCD. In ANN modelling, 13 runs of CCD matr...
Bioactive Natural products in Drug Discovery, 2020
Cinnamon (Ceylon cinnamon or true cinnamon) is a popular, expensive aromatic condiment and flavor... more Cinnamon (Ceylon cinnamon or true cinnamon) is a popular, expensive aromatic condiment and flavoring agent cum medicinal plant that houses a major number of pharmaceutically active aromatic essential oils and its principal compound cinnamaldehyde, and its derivatives cinnamic acid, cinnamate, etc. In addition to its culinary use, in ancient Ayurvedic texts as well as in folkloric medicine, this plant and precisely the bark and bark powder have been reported as a remedy for respiratory, digestive, and gynecological ailments. It has been evaluated clinically and preclinically for its prominent antioxidant, anti-inflammatory, antidiabetic, antimicrobial, anticancer, lipid-lowering, and cardio- and neuroprotective efficacies. Commercially this plant is highly valued in the pharmaceutical and cosmetic industry. We have summarized the recent relevant scientific evidence on the botanic characterization, distribution, traditional uses, pharmacological activities, phytochemistry, qualitative...
Bioactive Natural products in Drug Discovery, 2020
Swertia (Gentianaceae), a diverse genus, is mentioned as a potential herbal drug in Ayurvedic, Un... more Swertia (Gentianaceae), a diverse genus, is mentioned as a potential herbal drug in Ayurvedic, Unani, and Siddha traditional systems of medicine. Since time immemorial, 70 Swertia species have been used worldwide to cure several health illnesses associated with malaria, cancer, diabetes, inflammation, liver complications, different kinds of fever, etc. Swertia herb is used as the principal component in several marketed herbal/polyherbal formulations. Medicinal usage of Swertia is endorsed to the miscellaneous compounds, viz. xanthones, iridoids, seco-iridoids, and triterpenoids. Swertia is one of the most imperative trade herbs since its market value rises by 10% yearly. A chain of systematic isolation of bioactive compounds and their diverse range of pharmacological effects during the last 10–15 years proved this genus as an industrially important plant. This chapter makes an effort to present the comprehensive assessment on distribution, ethnopharmacology, biological activities, p...
BMC Plant Biology, 2021
Background Gloriosa superba L. (Colchicaceae) is a high-value medicinal plant indigenous to Afric... more Background Gloriosa superba L. (Colchicaceae) is a high-value medicinal plant indigenous to Africa and Southeast Asia. Its therapeutic benefits are well-established in traditional medicines including Ayurveda. It is well known for its natural bioactive compound colchicine which exhibits a wide range of pharmacological activities i.e. rheumatism, gout and was also introduced into clinical practices. The increasing demand as well as its illegal harvesting has brought this valuable plant under threatened category. Methods The present investigation describes a microwave assisted extraction (MAE) strategy coupled with a densitometric-high performance thin layer chromatographic (HPTLC) methodology for the analysis of colchicine from 32 different populations of G. superba . A Box-Behnken statistical design (3 level factor) has been employed to optimize MAE, in which power of microwave, time of irradiation, aqueous ethanol and pH were used as independent variables whereas colchicine was use...
Pharmacogn. J., 2016
Background: Alpinia galanga and Eryngium foetidum are two commonly used traditional aromatic plan... more Background: Alpinia galanga and Eryngium foetidum are two commonly used traditional aromatic plants of Manipur which is traditionally used in Aroma therapy. Rationale of pharmacological potentials of these plants are still unclear, even if few preliminary studies are available in literature for individual plants. Objective: This study was conducted for comparative assessment of antioxidant, antibacterial, and antidiabetic potential of A. galanga and E. foetidum. Materials and Methods: The rhizome of A. galanga and leaf of E. foetidum were extracted in methanol, ethanol and water. Phyto-chemicals of each extracts of Alpinia galanga and Eryngium foetidum were analyzed. The antioxidant potential of all the extracts was assessed by measuring total phenolic content, total flavonoid content and free radical scavenging potential was assessed by 1,1-diphenyl-2-picrilhydrazyl (DPPH) assay, antibacterial activity was assessed against various pathogenic and nonpathogenic bacteria in vitro by K...
Reserach Area I have carried out extensive research in the area of authentication and standardiza... more Reserach Area
I have carried out extensive research in the area of authentication and standardization of Medicinal and Aromatic plants by phytochemical and molecular techniques. I am well versed in chromatographic techniques i.e High performance thin layer chromatograph, High pressure liquid chromatography, Gas Chromatography as well as DNA based molecular marker technology. I have worked on RAPD, AFLP, ISSR and STMS markers for determination of genetic variability and identification of superior germplam of MAPs i.e Aloe vera, Gloriosa superba, Lantana camara, Ocimum sanctum, Bacopa monnieri , Rauwolfia serpentine, Stevia rebaudiana and Swertia chirata. In addition to routine testing of using Phytochemical and PCR based markers, I have standardized micropropagation protocols of various medicinal and aromatic plants (MAPs) such as Swertia chirata, Aloe vera, Stevia rebaudiana, Rauwolfia serpentine, Bacopa monnierie, Hemidesmus indicus,Dioscorea deltoidea, Bacopa monnierie, Aristolochia indica.
BMC Complementary Medicine and Therapies, 2020
Background Mahasudarshan Churna (MC) is a polyherbal Ayurvedic medicine that is employed in fever... more Background Mahasudarshan Churna (MC) is a polyherbal Ayurvedic medicine that is employed in fever (especially chronic type), cold and malaria, improvement of digestion and appetite, removes toxins from the blood, boosts immunity and protects against common bacterial infections. Methods Validation and quantification of oleanolic acid (OA), ursolic acid (UA), mangiferin (M), gallic acid (GA), quercetin (Q) and curcumin (C) in commercial MC formulations by HPTLC method. Mobile phase, hexane: ethyl acetate: acetone (16.4: 3.6: 0.2, v/v) was used for the separation of OA and UA; ethyl acetate: glacial acetic acid: formic acid: water (20: 2.2: 2.2: 5.2 v/v) for the development of M; and toluene: ethyl acetate: formic acid (13.5: 9: 0.6 v/v) for the separation of GA, Q and C in crude sample extracts. Visualization and scanning were performed at λ = 530 nm for OA and UA, at λ = 254 nm for M and at λ = 366 nm for GA, Q and C. In addition, HPLC-PDA analysis was used to confirm the HPTLC resul...
Scientific Reports, 2021
Swertia chirata Buch.-Ham. ex C.B. Clarke is an important medicinal plant used in various herbal ... more Swertia chirata Buch.-Ham. ex C.B. Clarke is an important medicinal plant used in various herbal formulations as it shows significant biological activities such as hepatoprotective, hypoglycemic, anti-inflammatory, antimalarial, antioxidant and anti-parkinson. C-glucosyl xanthone glycoside (mangiferin) is known as bio-marker compound of genus Swertia L. Development of efficient extraction methods of C-glucosyl xanthone mangiferin from Swertia chirata was attempted by optimizing the pre-harvest, post-harvest and extraction techniques by full factorial design. Firstly, a full factorial design was implemented to evaluate the single and interactive effects of pre-harvest (growth stage and plant part), post-harvest (drying condition and storage periods) followed by selection of best extraction technique such as heat reflux extraction (HRE), microwave assisted extraction (MAE) and ultrasound assistant extraction (UAE) at different solvent types on mangiferin yield. HPTLC and HPLC techniqu...
BMC Plant Biology, 2020
Background In this study, response surface methodology (RSM) and artificial neural network (ANN) ... more Background In this study, response surface methodology (RSM) and artificial neural network (ANN) was used to construct the predicted models of linear, quadratic and interactive effects of two independent variables viz. salicylic acid (SA) and chitosan (CS) for the production of amarogentin (I), swertiamarin (II) and mangiferin (III) from shoot cultures of Swertia paniculata Wall. These compounds are the major therapeutic metabolites in the Swertia plant, which have significant role and demand in the pharmaceutical industries. Results Present study highlighted that different concentrations of SA and CS elicitors substantially influenced the % yield of (I), (II) and (III) compounds in the shoot culture established on modified ½ MS medium (supplemented with 2.22 mM each of BA and KN and 2.54 mM NAA). In RSM, different response variables with linear, quadratic and 2 way interaction model were computed with five-factor-three level full factorial CCD. In ANN modelling, 13 runs of CCD matr...
Bioactive Natural products in Drug Discovery, 2020
Cinnamon (Ceylon cinnamon or true cinnamon) is a popular, expensive aromatic condiment and flavor... more Cinnamon (Ceylon cinnamon or true cinnamon) is a popular, expensive aromatic condiment and flavoring agent cum medicinal plant that houses a major number of pharmaceutically active aromatic essential oils and its principal compound cinnamaldehyde, and its derivatives cinnamic acid, cinnamate, etc. In addition to its culinary use, in ancient Ayurvedic texts as well as in folkloric medicine, this plant and precisely the bark and bark powder have been reported as a remedy for respiratory, digestive, and gynecological ailments. It has been evaluated clinically and preclinically for its prominent antioxidant, anti-inflammatory, antidiabetic, antimicrobial, anticancer, lipid-lowering, and cardio- and neuroprotective efficacies. Commercially this plant is highly valued in the pharmaceutical and cosmetic industry. We have summarized the recent relevant scientific evidence on the botanic characterization, distribution, traditional uses, pharmacological activities, phytochemistry, qualitative...
Bioactive Natural products in Drug Discovery, 2020
Swertia (Gentianaceae), a diverse genus, is mentioned as a potential herbal drug in Ayurvedic, Un... more Swertia (Gentianaceae), a diverse genus, is mentioned as a potential herbal drug in Ayurvedic, Unani, and Siddha traditional systems of medicine. Since time immemorial, 70 Swertia species have been used worldwide to cure several health illnesses associated with malaria, cancer, diabetes, inflammation, liver complications, different kinds of fever, etc. Swertia herb is used as the principal component in several marketed herbal/polyherbal formulations. Medicinal usage of Swertia is endorsed to the miscellaneous compounds, viz. xanthones, iridoids, seco-iridoids, and triterpenoids. Swertia is one of the most imperative trade herbs since its market value rises by 10% yearly. A chain of systematic isolation of bioactive compounds and their diverse range of pharmacological effects during the last 10–15 years proved this genus as an industrially important plant. This chapter makes an effort to present the comprehensive assessment on distribution, ethnopharmacology, biological activities, p...
BMC Plant Biology, 2021
Background Gloriosa superba L. (Colchicaceae) is a high-value medicinal plant indigenous to Afric... more Background Gloriosa superba L. (Colchicaceae) is a high-value medicinal plant indigenous to Africa and Southeast Asia. Its therapeutic benefits are well-established in traditional medicines including Ayurveda. It is well known for its natural bioactive compound colchicine which exhibits a wide range of pharmacological activities i.e. rheumatism, gout and was also introduced into clinical practices. The increasing demand as well as its illegal harvesting has brought this valuable plant under threatened category. Methods The present investigation describes a microwave assisted extraction (MAE) strategy coupled with a densitometric-high performance thin layer chromatographic (HPTLC) methodology for the analysis of colchicine from 32 different populations of G. superba . A Box-Behnken statistical design (3 level factor) has been employed to optimize MAE, in which power of microwave, time of irradiation, aqueous ethanol and pH were used as independent variables whereas colchicine was use...
Pharmacogn. J., 2016
Background: Alpinia galanga and Eryngium foetidum are two commonly used traditional aromatic plan... more Background: Alpinia galanga and Eryngium foetidum are two commonly used traditional aromatic plants of Manipur which is traditionally used in Aroma therapy. Rationale of pharmacological potentials of these plants are still unclear, even if few preliminary studies are available in literature for individual plants. Objective: This study was conducted for comparative assessment of antioxidant, antibacterial, and antidiabetic potential of A. galanga and E. foetidum. Materials and Methods: The rhizome of A. galanga and leaf of E. foetidum were extracted in methanol, ethanol and water. Phyto-chemicals of each extracts of Alpinia galanga and Eryngium foetidum were analyzed. The antioxidant potential of all the extracts was assessed by measuring total phenolic content, total flavonoid content and free radical scavenging potential was assessed by 1,1-diphenyl-2-picrilhydrazyl (DPPH) assay, antibacterial activity was assessed against various pathogenic and nonpathogenic bacteria in vitro by K...