sumedha bobade | MAHARASHTRA ANIMAL AND FISHERY SCIENCES UNVIERSITY NAGPUR (original) (raw)
Papers by sumedha bobade
Sortases are Gram-positive bacteria extracellular transpeptidase enzymes responsible for covalent... more Sortases are Gram-positive bacteria extracellular transpeptidase enzymes responsible for covalently attaching secreted proteins to the peptidoglycan cell wall. The name of the enzymes derived from the role the enzymes play in the protein sorting pathway, ‘sorting’ proteins into the cell wall compartment of Gram-positive bacteria. The peptidoglycan is composed of numerous glycan polymers made up of repeating chains of N-acetyl glucosamine and N-acetylmuramic acid which are cross linked to one another by peptide stems. The peptide stems are generally composed of five amino acids with an inter-peptide branch at position 3. Staphylococcus aureus comprises L-Ala-D-iGlu-L-Lys-DAlaD-Ala with a pentaglycine inter-peptide branching off the L-Lys. These peptide cross-links between the glycan strands vary between Gram-positive and Gram negative bacteria in the inter peptide branching region. Based on the primary sequences around 60% of sortase homologues in Gram-positive bacteria can be cluste...
International Journal of Health Sciences and Research, 2016
Listeria monocytogenes is food-borne bacterial pathogen and listeriosis, a public health importan... more Listeria monocytogenes is food-borne bacterial pathogen and listeriosis, a public health important disease which can cause an abortion in pregnant women. Listeriosis is emerging as worldwide bacterial zoonotic infection caused by Listeria monocytogenes a facultative intracellular bacterium causative agent of listeriosis. In present investigation aim for study of prevalence, virulence gene profiling and serotyping of Listeria monocytogenes isolates by employing multiplex and serotyping polymerase chain reaction. Total 113 samples, comprising vaginal (n=66) and endocervical (n=47) swabs were collected aseptically for isolation recovered from infertility cases of women. These isolates were subjected to amplify virulence-associated gens namely plcA, actA, hlyA and iap by multiplex and prf A gene by singleplex PCR. Serotyping PCR was adopted for further genotypic characterization. Most of the isolates revealed amplification of actA, hlyA and prf A gene. Serotyping of recovered 11 Listeri...
Agricultural Reviews
Ancient virus genomes preserved as fossils and carried by host within their genome. Although vira... more Ancient virus genomes preserved as fossils and carried by host within their genome. Although viral genomes evolve rapidly, their rate of change slows to the same pace as that of the host’s DNA after insertion, making it possible to study viral DNA sequences that are many millions of years old. Paleovirology is the study of viral fossil records typically over prehistoric or geological timescales and the effects that these agents have had on the evolution of their hosts. Viruses sometimes heritably integrate into the genomes of their hosts, resulting in genomic features known as endogenous viral elements (EVEs). Using EVEs, the field of paleovirology investigates the long term evolution of viruses and their impact on hosts. One of the fruitful outcomes of high throughput genomics is the widespread availability of whole genome data, offering the unprecedented opportunity to investigate EVEs at a large scale. This review, highlights the utility of antiviral gene evolution for the study ...
Bioscience Biotechnology Research Communications
The natural biosensors are chemical sense organs specially designed on the basis of smell and tas... more The natural biosensors are chemical sense organs specially designed on the basis of smell and taste likewise. Biosensor is a device that detects, transmits and records information regarding physiological or biochemical changes. Basically it is the probe that integrates a biological component with an electronic transducer thereby converting biochemical signals into electrochemical, optical, acoustic and electronic ones. The function of a biosensor depends on specifi city of biological active material and the analyte to be detected such as chemical compound, antigen, microbes, hormones, nucleic acid or any subjective parameter like smell and taste. The biological sensing elements have been used as enzyme, antibody, DNA ,receptor ,organelles and microorganism as well as animal and plant tissues. Types of biosensor includes immunosensors, microbial biosensors, whole cell based, electrochemical, optical and acoustic biosensors, which have vast applications in biomedical research, healthcare, pharmaceutical, environmental monitoring, homemade security and battlefi elds. In this review a summary of relevant aspects concerning biosensor integration in effi cient analytical setups and the latest applications of biosensors in diagnostic applications focusing on detection of molecular biomarkers in real samples is included. An overview of the current state and future trends of biosensors in this fi eld is given.
Sortases are Gram-positive bacteria extracellular transpeptidase enzymes responsible for covalent... more Sortases are Gram-positive bacteria extracellular transpeptidase enzymes responsible for covalently attaching secreted proteins to the peptidoglycan cell wall. The name of the enzymes derived from the role the enzymes play in the protein sorting pathway, ‘sorting’ proteins into the cell wall compartment of Gram-positive bacteria. The peptidoglycan is composed of numerous glycan polymers made up of repeating chains of N-acetyl glucosamine and N-acetylmuramic acid which are cross linked to one another by peptide stems. The peptide stems are generally composed of five amino acids with an inter-peptide branch at position 3. Staphylococcus aureus comprises L-Ala-D-iGlu-L-Lys-DAlaD-Ala with a pentaglycine inter-peptide branching off the L-Lys. These peptide cross-links between the glycan strands vary between Gram-positive and Gram negative bacteria in the inter peptide branching region. Based on the primary sequences around 60% of sortase homologues in Gram-positive bacteria can be cluste...
International Journal of Health Sciences and Research, 2016
Listeria monocytogenes is food-borne bacterial pathogen and listeriosis, a public health importan... more Listeria monocytogenes is food-borne bacterial pathogen and listeriosis, a public health important disease which can cause an abortion in pregnant women. Listeriosis is emerging as worldwide bacterial zoonotic infection caused by Listeria monocytogenes a facultative intracellular bacterium causative agent of listeriosis. In present investigation aim for study of prevalence, virulence gene profiling and serotyping of Listeria monocytogenes isolates by employing multiplex and serotyping polymerase chain reaction. Total 113 samples, comprising vaginal (n=66) and endocervical (n=47) swabs were collected aseptically for isolation recovered from infertility cases of women. These isolates were subjected to amplify virulence-associated gens namely plcA, actA, hlyA and iap by multiplex and prf A gene by singleplex PCR. Serotyping PCR was adopted for further genotypic characterization. Most of the isolates revealed amplification of actA, hlyA and prf A gene. Serotyping of recovered 11 Listeri...
Agricultural Reviews
Ancient virus genomes preserved as fossils and carried by host within their genome. Although vira... more Ancient virus genomes preserved as fossils and carried by host within their genome. Although viral genomes evolve rapidly, their rate of change slows to the same pace as that of the host’s DNA after insertion, making it possible to study viral DNA sequences that are many millions of years old. Paleovirology is the study of viral fossil records typically over prehistoric or geological timescales and the effects that these agents have had on the evolution of their hosts. Viruses sometimes heritably integrate into the genomes of their hosts, resulting in genomic features known as endogenous viral elements (EVEs). Using EVEs, the field of paleovirology investigates the long term evolution of viruses and their impact on hosts. One of the fruitful outcomes of high throughput genomics is the widespread availability of whole genome data, offering the unprecedented opportunity to investigate EVEs at a large scale. This review, highlights the utility of antiviral gene evolution for the study ...
Bioscience Biotechnology Research Communications
The natural biosensors are chemical sense organs specially designed on the basis of smell and tas... more The natural biosensors are chemical sense organs specially designed on the basis of smell and taste likewise. Biosensor is a device that detects, transmits and records information regarding physiological or biochemical changes. Basically it is the probe that integrates a biological component with an electronic transducer thereby converting biochemical signals into electrochemical, optical, acoustic and electronic ones. The function of a biosensor depends on specifi city of biological active material and the analyte to be detected such as chemical compound, antigen, microbes, hormones, nucleic acid or any subjective parameter like smell and taste. The biological sensing elements have been used as enzyme, antibody, DNA ,receptor ,organelles and microorganism as well as animal and plant tissues. Types of biosensor includes immunosensors, microbial biosensors, whole cell based, electrochemical, optical and acoustic biosensors, which have vast applications in biomedical research, healthcare, pharmaceutical, environmental monitoring, homemade security and battlefi elds. In this review a summary of relevant aspects concerning biosensor integration in effi cient analytical setups and the latest applications of biosensors in diagnostic applications focusing on detection of molecular biomarkers in real samples is included. An overview of the current state and future trends of biosensors in this fi eld is given.