Neeraj Kashyap | Guru Angad Dev Veterinary And Animal Sciences University (original) (raw)

Papers by Neeraj Kashyap

Present study was conducted to compare the biochemical parameters of broiler birds’ in an open-si... more Present study was conducted to compare the biochemical parameters of broiler birds’ in an open-sided house under supplemental lighting programme using light emitting diodes (LEDs) and incandescent light. For this purpose 240 straight run commercial ven cobb broiler chicks were procured and distributed in four different light treatment groups i.e. T1; white (650nm), T2; green (565nm), T3; blue (430nm) light LED (3 Watt each) and incandescent light bulbs (60 Watt each) as control (Tc) in 12 pens (4x3 factorial design). At the day time, open-sided house was open from 10.00 AM to 04.00 PM and rest of the time sides of the house was covered with the black coloured tarpaulin sheet. Biochemical parameters (serum Ig, glucose, total protein, BUN, GPX, SOD, catalase and lipid peroxidation) were estimated from randomly collected blood from 3 birds of each replicate of each treatment at 7, 21 and 42 day of experiment period. Blood biochemical evaluation indicated that the level of total immunog...

International Journal of Current Microbiology and Applied Sciences, 2019

3 Biotech, 2019

In the current study, we have synthesized canine recombinant Hsp27 in E. coli and raised hyperimm... more In the current study, we have synthesized canine recombinant Hsp27 in E. coli and raised hyperimmune serum against the protein in mice. Using the hyperimmune serum, an indirect ELISA was developed to estimate circulating levels of Hsp27 in dogs with various types of mammary neoplasia and to compare their levels with those of tumor-free apparently healthy dogs. The developed assay had a high specificity (83.3%) and sensitivity (96.6%) for a cutoff value of 0.45 with respect to histopathological examination in discriminating healthy dogs from those bearing mammary tumors. Serum Hsp27 levels were found to be significantly elevated in tumor subjects (20.33 ± 1.23 ng/ml) as compared to healthy controls (6.56 ± 0.51 ng/ml) and the increase in the Hsp27 levels was irrespective of mammary tumor histotypes. However, dogs with grade-II tumors had higher Hsp27 levels as compared to grade-I types. Therefore, Hsp27 can be exploited as one of the 'neoplastic signatures' of canine mammary tumors.

Indian Journal of Animal Research, 2015

During the process of increasing production potential of poultry, other economically important tr... more During the process of increasing production potential of poultry, other economically important traits like disease resistance are neglected and ultimately leading to decrease in quality food production. The poultry health management is important due to emergence of highly pathogenic diseases like Avian Influenza. Mx1 gene has been reported to provide resistance against avian influenza virus in chicken. Therefore, present research work was focused to identify the genetic variation of Mx1 gene in Japanese quail. Total three fragments viz. Frag-I of 185 bp (Exon 3 region), Frag-II of 161 bp (Exon 7 region) and Frag-III of 176 bp (Exon 13 region) of Mx1 gene in 170 Japanese quail birds were amplified and screened for polymorphism by PCR-SSCP technique. All the three fragments were found to be monomorphic which was confirmed by dideoxy nucleotide sequencing. Comparative analysis of Mx1 sequence of Japanese quail by MegAlign programme of DNASTAR showed 100% homology with common quail and ...

Veterinary World, 2015

Aim: One of the major biochemical aspects of thermoregulation is equilibrium of ion gradient acro... more Aim: One of the major biochemical aspects of thermoregulation is equilibrium of ion gradient across biological membranes. Na + /K +-ATPase, a member of P type-ATPase family, is a major contributor to the mechanism that actively controls crossmembrane ion gradient. Thus, we examined ATP1A1 gene that encodes alpha-1 chain of Na + /K +-ATPase, for genetic polymorphisms. Materials and Methods: A total of 100 Vrindavani (composite cross strain of Hariana x Holstein-Friesian/Brown Swiss/ Jersey) and 64 Tharparkar (indigenous) cattle were screened for genetic polymorphism in ATP1A1 gene, using polymerase chain reaction single-strand conformation polymorphism and DNA sequencing. For association studies, rectal temperature (RT) and respiration rate (RR) of all animals were recorded twice daily for 3 seasons. Results: A SNP (C2789A) was identified in exon 17 of ATP1A1 gene. Three genotypes namely CC, CA, and AA were observed in both, Vrindavani and Tharparkar cattle. The gene frequencies in Tharparkar and Vrindavani for allele A were 0.51 and 0.48, and for allele C were 0.49 and 0.52, respectively, which remained at intermediate range. Association study of genotypes with RT and RR in both cattle population revealed that the animals with genotype CC exhibited significantly lower RT and higher heat tolerance coefficient than CA and AA genotypes. Conclusion: Differential thermoregulation between different genotypes of ATP1A1 gene indicate that the ATP1A1 gene could be potentially contributing to thermotolerance in both, Tharparkar, an indigenous breed and Vrindavani, a composite crossbred cattle.

Indian Journal of Animal Research, 2015

A total number of 76 randomly selected birds i.e. 20 of Hill fowl (HF), 14 of Rhode Island Red (R... more A total number of 76 randomly selected birds i.e. 20 of Hill fowl (HF), 14 of Rhode Island Red (RIR), 14 of Kadaknath (KN), 14 of White LeghWDDorn (WLH) and 14 of White Cornish (WC) were genotyped using 25 microsatellite markers in the present study. Out of 25 microsatellite loci, 17 (~70%) were found to be polymorphic among these breeds. Across the breeds, total number of alleles ranged from 2 to 3 at polymorphic locus and average number of alleles per locus was 2.41. The allele size ranged between 98 bp and 340 bp. Among all the polymorphic microsatellite loci, observed heterozygosity were 0.441, 0.415, 0.287, 0.296 and 0.376 and expected heterozygosity were 0.435, 0.443, 0.387, 0.384 and 0.442 in HF, RIR, KN, WLH and WC, respectively. Polymorphic Information Content (PIC) estimates, across all the polymorphic loci were 0.346, 0.342, 0.305, 0.297 and 0.330 in HF, RIR, KN, WLH and WC, respectively.

Asian-Australasian Journal of Animal Sciences, 2015

Heat stress has adverse effects on fertility of dairy animals. Decline in fertility is linearly a... more Heat stress has adverse effects on fertility of dairy animals. Decline in fertility is linearly associated with an increase in combination of both temperature and humidity. The purpose of this study was to investigate the relationship between temperature humidity index (THI) and the pregnancy rate of Murrah buffaloes in a subtropical climate. The effects of genetic and non-genetic factors viz., sire, parity, period of calving and age group at first calving were found non-significant on pregnancy rate. The effect of THI was found significant (p<0.001) on pregnancy rate of Murrah buffaloes calved for first time and overall pregnancy rate. The threshold THI affecting the pregnancy rate was identified as THI 75. The months from October to March showed THI<75 and considered as non heat stress zone (NHSZ), while months from April to September were determined as heat stress zone (HSZ) with THI≥75. The lowest overall pregnancy rate (0.25) was obtained in July with THI 80.9, while the highest overall pregnancy rate (0.59) was found in November with THI 66.1. May and June were identified as critical heat stress zone (CHSZ) within the HSZ with maximum decline (-7%) in pregnancy rate with per unit increase in THI. The highest overall pregnancy rate was estimated as 0.45 in NHSZ with THI value 56.7 to 73.2. The pregnancy rate was found to have declined to 0.28 in HSZ with THI 73.5 to 83.7. However, the lowest pregnancy rate was estimated as 0.27 in CHSZ with THI value 80.3 to 81.6.

Indian Journal of Animal Research, 2021

Background: The study was conducted to unravel the consequences of abnormal lactation lengths (Ex... more Background: The study was conducted to unravel the consequences of abnormal lactation lengths (Extremely short, short, prolong and extremely prolong lactation length) on production and reproduction traits of crossbred cattle (Red Dane x Sahiwal x Holstein Friesian), which otherwise remains unutilized in routine breeding data analysis owing to normalization and standardization of lactation lengths. Methods: The performance data of 2541 lactations of 1001 crossbred cattle, sired by 146 bulls over a period of 30 years maintained at the Livestock Farms of Guru Angad Dev Veterinary and Animal Sciences University (GADVASU), Ludhiana were used for this study. The data on production and reproduction traits were analysed using general linear model procedures based on extremely short ( less than 102 days), short (102-179 days), prolong (483 - 560 days) and extremely prolong (≥ 560 days) lactation lengths on the basis of mean lactation length and its standard deviation. Result: Result indicate...

Veterinary World, 2016

Aim: Out of various members of heat shock protein (HSP) superfamily which act a molecular chapero... more Aim: Out of various members of heat shock protein (HSP) superfamily which act a molecular chaperon by binding to the denaturing protein thus stabilizing them and preserving their activity, HSP70 are of major importance in thermotolerance development. Thus, present investigation aimed at a screening of HSP70 gene for polymorphisms and possible differences in thermotolerance in Tharparkar breed of cattle. Materials and Methods: A 295 bp fragment of HSP70 gene was subjected to polymerase chain reaction-singlestrand conformation polymorphism (SSCP) followed by sequencing of different SSCP patterns in 64 Tharparkar cattle. A comparative thermotolerance of identified genotypes was analyzed using heat tolerance coefficients (HTCs) of animals for different seasons. Results: Three SSCP patterns and consequently two alleles namely A and B were documented in one fragment of HSP70 gene. On sequencing, one single-nucleotide polymorphism with G > T substitution was found at a position that led to a change of amino acid aspartate to tyrosine in allele A. It was found that in maintaining near normal average rectal temperature, genotype AA was superior (p≤0.01). Genotype AA, thus, was found to be most thermotolerant genotype with the highest HTC (p≤0.01). Conclusion: The polymorphism at HSP70 is expected to be a potent determinant for heat tolerance in cattle, which may aid in selection for thermotolerance in cattle.

Present study was conducted to compare the biochemical parameters of broiler birds’ in an open-si... more Present study was conducted to compare the biochemical parameters of broiler birds’ in an open-sided house under supplemental lighting programme using light emitting diodes (LEDs) and incandescent light. For this purpose 240 straight run commercial ven cobb broiler chicks were procured and distributed in four different light treatment groups i.e. T1; white (650nm), T2; green (565nm), T3; blue (430nm) light LED (3 Watt each) and incandescent light bulbs (60 Watt each) as control (Tc) in 12 pens (4x3 factorial design). At the day time, open-sided house was open from 10.00 AM to 04.00 PM and rest of the time sides of the house was covered with the black coloured tarpaulin sheet. Biochemical parameters (serum Ig, glucose, total protein, BUN, GPX, SOD, catalase and lipid peroxidation) were estimated from randomly collected blood from 3 birds of each replicate of each treatment at 7, 21 and 42 day of experiment period. Blood biochemical evaluation indicated that the level of total immunog...

International Journal of Current Microbiology and Applied Sciences, 2019

3 Biotech, 2019

In the current study, we have synthesized canine recombinant Hsp27 in E. coli and raised hyperimm... more In the current study, we have synthesized canine recombinant Hsp27 in E. coli and raised hyperimmune serum against the protein in mice. Using the hyperimmune serum, an indirect ELISA was developed to estimate circulating levels of Hsp27 in dogs with various types of mammary neoplasia and to compare their levels with those of tumor-free apparently healthy dogs. The developed assay had a high specificity (83.3%) and sensitivity (96.6%) for a cutoff value of 0.45 with respect to histopathological examination in discriminating healthy dogs from those bearing mammary tumors. Serum Hsp27 levels were found to be significantly elevated in tumor subjects (20.33 ± 1.23 ng/ml) as compared to healthy controls (6.56 ± 0.51 ng/ml) and the increase in the Hsp27 levels was irrespective of mammary tumor histotypes. However, dogs with grade-II tumors had higher Hsp27 levels as compared to grade-I types. Therefore, Hsp27 can be exploited as one of the 'neoplastic signatures' of canine mammary tumors.

Indian Journal of Animal Research, 2015

During the process of increasing production potential of poultry, other economically important tr... more During the process of increasing production potential of poultry, other economically important traits like disease resistance are neglected and ultimately leading to decrease in quality food production. The poultry health management is important due to emergence of highly pathogenic diseases like Avian Influenza. Mx1 gene has been reported to provide resistance against avian influenza virus in chicken. Therefore, present research work was focused to identify the genetic variation of Mx1 gene in Japanese quail. Total three fragments viz. Frag-I of 185 bp (Exon 3 region), Frag-II of 161 bp (Exon 7 region) and Frag-III of 176 bp (Exon 13 region) of Mx1 gene in 170 Japanese quail birds were amplified and screened for polymorphism by PCR-SSCP technique. All the three fragments were found to be monomorphic which was confirmed by dideoxy nucleotide sequencing. Comparative analysis of Mx1 sequence of Japanese quail by MegAlign programme of DNASTAR showed 100% homology with common quail and ...

Veterinary World, 2015

Aim: One of the major biochemical aspects of thermoregulation is equilibrium of ion gradient acro... more Aim: One of the major biochemical aspects of thermoregulation is equilibrium of ion gradient across biological membranes. Na + /K +-ATPase, a member of P type-ATPase family, is a major contributor to the mechanism that actively controls crossmembrane ion gradient. Thus, we examined ATP1A1 gene that encodes alpha-1 chain of Na + /K +-ATPase, for genetic polymorphisms. Materials and Methods: A total of 100 Vrindavani (composite cross strain of Hariana x Holstein-Friesian/Brown Swiss/ Jersey) and 64 Tharparkar (indigenous) cattle were screened for genetic polymorphism in ATP1A1 gene, using polymerase chain reaction single-strand conformation polymorphism and DNA sequencing. For association studies, rectal temperature (RT) and respiration rate (RR) of all animals were recorded twice daily for 3 seasons. Results: A SNP (C2789A) was identified in exon 17 of ATP1A1 gene. Three genotypes namely CC, CA, and AA were observed in both, Vrindavani and Tharparkar cattle. The gene frequencies in Tharparkar and Vrindavani for allele A were 0.51 and 0.48, and for allele C were 0.49 and 0.52, respectively, which remained at intermediate range. Association study of genotypes with RT and RR in both cattle population revealed that the animals with genotype CC exhibited significantly lower RT and higher heat tolerance coefficient than CA and AA genotypes. Conclusion: Differential thermoregulation between different genotypes of ATP1A1 gene indicate that the ATP1A1 gene could be potentially contributing to thermotolerance in both, Tharparkar, an indigenous breed and Vrindavani, a composite crossbred cattle.

Indian Journal of Animal Research, 2015

A total number of 76 randomly selected birds i.e. 20 of Hill fowl (HF), 14 of Rhode Island Red (R... more A total number of 76 randomly selected birds i.e. 20 of Hill fowl (HF), 14 of Rhode Island Red (RIR), 14 of Kadaknath (KN), 14 of White LeghWDDorn (WLH) and 14 of White Cornish (WC) were genotyped using 25 microsatellite markers in the present study. Out of 25 microsatellite loci, 17 (~70%) were found to be polymorphic among these breeds. Across the breeds, total number of alleles ranged from 2 to 3 at polymorphic locus and average number of alleles per locus was 2.41. The allele size ranged between 98 bp and 340 bp. Among all the polymorphic microsatellite loci, observed heterozygosity were 0.441, 0.415, 0.287, 0.296 and 0.376 and expected heterozygosity were 0.435, 0.443, 0.387, 0.384 and 0.442 in HF, RIR, KN, WLH and WC, respectively. Polymorphic Information Content (PIC) estimates, across all the polymorphic loci were 0.346, 0.342, 0.305, 0.297 and 0.330 in HF, RIR, KN, WLH and WC, respectively.

Asian-Australasian Journal of Animal Sciences, 2015

Heat stress has adverse effects on fertility of dairy animals. Decline in fertility is linearly a... more Heat stress has adverse effects on fertility of dairy animals. Decline in fertility is linearly associated with an increase in combination of both temperature and humidity. The purpose of this study was to investigate the relationship between temperature humidity index (THI) and the pregnancy rate of Murrah buffaloes in a subtropical climate. The effects of genetic and non-genetic factors viz., sire, parity, period of calving and age group at first calving were found non-significant on pregnancy rate. The effect of THI was found significant (p<0.001) on pregnancy rate of Murrah buffaloes calved for first time and overall pregnancy rate. The threshold THI affecting the pregnancy rate was identified as THI 75. The months from October to March showed THI<75 and considered as non heat stress zone (NHSZ), while months from April to September were determined as heat stress zone (HSZ) with THI≥75. The lowest overall pregnancy rate (0.25) was obtained in July with THI 80.9, while the highest overall pregnancy rate (0.59) was found in November with THI 66.1. May and June were identified as critical heat stress zone (CHSZ) within the HSZ with maximum decline (-7%) in pregnancy rate with per unit increase in THI. The highest overall pregnancy rate was estimated as 0.45 in NHSZ with THI value 56.7 to 73.2. The pregnancy rate was found to have declined to 0.28 in HSZ with THI 73.5 to 83.7. However, the lowest pregnancy rate was estimated as 0.27 in CHSZ with THI value 80.3 to 81.6.

Indian Journal of Animal Research, 2021

Background: The study was conducted to unravel the consequences of abnormal lactation lengths (Ex... more Background: The study was conducted to unravel the consequences of abnormal lactation lengths (Extremely short, short, prolong and extremely prolong lactation length) on production and reproduction traits of crossbred cattle (Red Dane x Sahiwal x Holstein Friesian), which otherwise remains unutilized in routine breeding data analysis owing to normalization and standardization of lactation lengths. Methods: The performance data of 2541 lactations of 1001 crossbred cattle, sired by 146 bulls over a period of 30 years maintained at the Livestock Farms of Guru Angad Dev Veterinary and Animal Sciences University (GADVASU), Ludhiana were used for this study. The data on production and reproduction traits were analysed using general linear model procedures based on extremely short ( less than 102 days), short (102-179 days), prolong (483 - 560 days) and extremely prolong (≥ 560 days) lactation lengths on the basis of mean lactation length and its standard deviation. Result: Result indicate...

Veterinary World, 2016

Aim: Out of various members of heat shock protein (HSP) superfamily which act a molecular chapero... more Aim: Out of various members of heat shock protein (HSP) superfamily which act a molecular chaperon by binding to the denaturing protein thus stabilizing them and preserving their activity, HSP70 are of major importance in thermotolerance development. Thus, present investigation aimed at a screening of HSP70 gene for polymorphisms and possible differences in thermotolerance in Tharparkar breed of cattle. Materials and Methods: A 295 bp fragment of HSP70 gene was subjected to polymerase chain reaction-singlestrand conformation polymorphism (SSCP) followed by sequencing of different SSCP patterns in 64 Tharparkar cattle. A comparative thermotolerance of identified genotypes was analyzed using heat tolerance coefficients (HTCs) of animals for different seasons. Results: Three SSCP patterns and consequently two alleles namely A and B were documented in one fragment of HSP70 gene. On sequencing, one single-nucleotide polymorphism with G > T substitution was found at a position that led to a change of amino acid aspartate to tyrosine in allele A. It was found that in maintaining near normal average rectal temperature, genotype AA was superior (p≤0.01). Genotype AA, thus, was found to be most thermotolerant genotype with the highest HTC (p≤0.01). Conclusion: The polymorphism at HSP70 is expected to be a potent determinant for heat tolerance in cattle, which may aid in selection for thermotolerance in cattle.