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Papers by Adisak Wongkajornsilp

Immunology, 2019

Natural killer T (NKT) cells and cytokine-induced killer (CIK) cells contain features of both T c... more Natural killer T (NKT) cells and cytokine-induced killer (CIK) cells contain features of both T cells and NK cells that occasionally brought confusion to several studies. These cells have been reported to possess either anti-tumor or immunosuppressive properties. NKT cells could be directly isolated from the peripheral blood, while CIK cells require ex vivo culture of peripheral blood lymphocytes for 2-3 weeks with IFN-γ, IL2 and anti-CD3. NKT type I cells carry invariant TCRαβ recognizing α-Galcer loaded CD1d on antigen-presenting cells. We investigated whether NKT type I cells, as well as CIK cells, could be induced toward Th1 phenotype that should elicit anti-tumor activity. The inducers included ODN, checkpoint inhibitors and CDKi. We observed the tipping toward Th1 phenotype in both cell types, especially on CIK cells. It is concluded that both NKT type I cells and CIK cells could be induced toward the anti-tumor Th1 phenotype. Citation Format: Adisak Wongkajornsilp, Khin Su Su Htwe, Nathawadee Sawatpiboon, Sunisa Duangsa-ard, Kanda Kasetsinsombat. The induction of iNKT cells and CIK cells toward anti-tumor phenotypes [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2019; 2019 Mar 29-Apr 3; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2019;79(13 Suppl):Abstract nr 4141.

Cancer research, Mar 22, 2024

Pharmaceutical Sciences Asia, 2022

Journal of Asthma and Allergy, Apr 1, 2023

PubMed, Oct 1, 2016

Background: Bone marrow (BM), which is a good source of stem cells and biological factors, has th... more Background: Bone marrow (BM), which is a good source of stem cells and biological factors, has the potential to enhance bone fusion. Simple centrifugation technique is one of the procedures used to concentrate BM aspirate for increasing number of cells. However, there are limited clinical study for using BM concentrate augmentation in spinal fusion. Objective: This study was designed to examine the spinal fusion enhancement effects of bone marrow (BM) concentrate augmentation on poster lateral lumbar fusion (PLF) with autologous local bone graft in terms of both quality and quantity, as compared with a control procedure without BM concentrate augmentation. Material and method: Twelve patients with L4-L5 spondylolisthesis scheduled for PLF after decompressive laminectomy and pedicle screw instrumentation were included in this study. This prospective randomized controlled trial was conducted at Siriraj Hospital during the 2009 to 2012 study period. Patients were randomly assigned to two groups. One group underwent PLF with local bone graft with BM concentrate augmentation (BM group) and the other group underwent PLF with local bone graft only (non-BM group). Clinical outcomes were evaluated by the Oswestry Disability Index (ODI) preoperatively and at 3 and 6 months after PLF. Bone fusion quality was evaluated by bony bridging on 3D-CT imaging. Fusion mass volumes were measured on quantitative 3D-CT scans at 1 week and 6 months, postoperatively. Results: Clinical outcome scores did not differ between groups. Six-month postoperative 3D-CT imaging showed complete PLF bridging in 58.3% and 100% of patients in the BM and non-BM groups, respectively. PLF mass volumes were decreased at 6 months by 51.1% in the BM group and by 48.5% in the non-BM group. One patient in the BM group had local inflammation at the BM aspiration site. Conclusion: Bone marrow concentrate augmentation in this small randomized controlled trial failed to demonstrate positive effects on autologous local bone graft in posterolateral lumbar fusion relative to both quality and quantity. The high percentage of incomplete bridging should also be noted and further investigated.

African Journal of Traditional, Complementary and Alternative Medicines, May 4, 2009

The objectives of this work were firstly, the screening for aw in liquid and dry herbal extracts ... more The objectives of this work were firstly, the screening for aw in liquid and dry herbal extracts (20 and 24 samples respectively), from 5 different manufacturers and secondly, relating aw variations to moisture content changes of dry extracts exposed to different ambientes (three controlled humidity chambers at 98, 76 and 50% RH, at 20oC). Aw was measured with a Dew Point Hygrometer. First results showed that aw values ranged from 0,190 to 0,420 and from 0,780 to 0,999 in dried and fluid extracts respectively and secondly as soon as the dried extracts were exposed to higher RH, a sudden increment of aw was observed while the rise of moisture content was smoother and delayed with respect to aw. The lower the initial aw value of the sample, the steeper was the increment. After 3-4 hours of exposure to 98% RH, aw reached, in some cases, values beyond 0,60 (moulds and yeasts can start developing). Also, glass transition was observed at 76% and 98% RH. This study shows that the exposure of dried herbal extracts to ambient conditions may lead to increment of aw that can affect the quality and shelf-life of the product. It also seems that aw is a more suitable tool than the moisture content for HACCP. Further research is needed to evaluate the possible moisture absorption from the ambiente that can occur when, packaging is not well sealed or/and they are exposed to moist environments, moist manufacturing and mixtures with products of higher aw, among others.

วารสารเภสัชวิทยา (Thai Journal of Pharmacology), 1999

Cancer Research, Apr 4, 2023

Clinical trials on cholangiocarcinoma revealed differential sensitivity to cytolytic action of al... more Clinical trials on cholangiocarcinoma revealed differential sensitivity to cytolytic action of allogeneic cytokine-induced killer (CIK) cells. Different metastatic foci in the same individual could also display different sensitivity. The differential sensitivity of separate lines of cultured cholangiocarcinoma cells could be demonstrated based on the evaluation of GR50. Allogeneic CIK cell or CD3+CD56+ subset was co-cultured with nine cell lines at E:T ratio of 20:1 for 48 h. The tumor cells were categorized into 2 groups based on the obtained GR50. This resulted in 4 sensitive cell lines and 5 resistant cell lines. The cholangiocarcinoma cell-exposed CIK cell and CD3+CD56+ subset displayed defective anti-tumor cytotoxicity upon subsequent experiment. RNAseq analysis revealed 261 genes upregulated in sensitive cell lines and 665 genes upregulated in resistant cell lines. The top 50 resistant genes were evaluated for their linked signal transduction and protein interaction network. Based on the interaction network, a list of chemicals that could interrupt the stipulated network were selected. They were assayed for their synergistic activity with dysfunctional CIK cell or CD3+CD56+ subset for cytolysis of resistant tumor cells. Citation Format: Adisak Wongkajornsilp, Khin Su Su Htwe, Porncheera Chusorn, Sunisa Duangsa-ard, Kanda Kasetsinsombat, Nathawadee Sawatpiboon. Reversal of dysfunctional CIK cells after the exposure to resistant cholangiocarcinoma cells through chemical perturbation of resistant gene signature [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr 6351.

Siriraj Medical Journal, May 1, 2022

Approximately 20 new cases of glioblastoma multiforme have been diagnosed annually at Siriraj Hos... more Approximately 20 new cases of glioblastoma multiforme have been diagnosed annually at Siriraj Hospital. Glioblastoma multiforme is the most invasive of the astroglial neoplasms with a tendency to spread widely throughout the brain. Glioblastoma is very often extensively spread by the time of diagnosis, leading to unresectability, inadequate local control and a consistently poor prognosis. The length and quality of survival have improved only minimally through neurosurgical technique, radiotherapy and chemotherapy. Radical surgery and radiotherapy remain the cornerstone of treatment. A very modest contribution is added by conventional chemotherapy. Therefore, a novel therapeutic model is required for this cancer. We proposed that OK-432, a heat-treated and penicillin-treated lyophilized powder of the Su substrain of Streptococcus pyogenes A3, could be used as an immunomodulating agent to treat this cancer. It has a biological response modifier like BCG. Addition of OK-432 to peripher...

Objective: To compare sonication and homogenization methods for preparation of mosquito allergen ... more Objective: To compare sonication and homogenization methods for preparation of mosquito allergen extracts. Materials and Methods: Mosquito allergens were extracted from Culex quinquefasciatus using two different methods, sonication and homogenization. Protein concentrations, protein profiles, and endotoxin levels in the extracts prepared by the two methods were determined. Results: The protein concentrations measured by Bradford (results in mg/mL) and Kjeldahl (results in Protein Nitrogen Units, PNU) assays in the extracts prepared by the sonication method were 0.92 0.08 mg/mL and 74700 4934 PNU, respectively. These results were significantly higher than those in the extracts prepared by the homogenization method (0.64 0.01 mg/mL and 41330 8231 PNU). The protein fingerprint showed more protein in the extracts prepared by sonication than in those prepared by homogenization. The endotoxin levels in the extracts from both methods were clinically acceptable, and there was no bacterial c...

Cancer research, Mar 22, 2024

Journal of the Medical Association of Thailand = Chotmaihet thangphaet, 2012

Giant cell tumor of bone is a common benign aggressive bone tumor. Recurrent rate in the patients... more Giant cell tumor of bone is a common benign aggressive bone tumor. Recurrent rate in the patients who have the tumor with soft tissue invasion is rather high. Use of strong chemicals such as phenol and liquid nitrogen to lessen the recurrent rate might not be used in this lesion. The experiment was carried out to discover apoptotic effects of thermoablation on giant cell tumor of bone to find out a possibility to use thermoablation in the clinic in the patients with extensive lesion. Tumor cell suspension was prepared from 4 patients who had definite diagnosis of giant cell tumor of bone. The tumor cells were subjected to thermoablation at 45, 47 and 50 degrees C for 10 to 30 minutes before were cultured at 37 degrees C for another 3 days. Osteoblasts and chondrocytes from the last 3 patients were collected,prepared and underwent thermoablation in the same fashion. Apoptosis of tumor cells, chondrocytes and osteoblasts were carried out by the use of flow cytometry. Thermoablation at...

Viruses, 2019

More than 350 million people worldwide have been persistently infected with the hepatitis B virus... more More than 350 million people worldwide have been persistently infected with the hepatitis B virus (HBV). Chronic HBV infection could advance toward liver cirrhosis and hepatocellular carcinoma. The intervention with prophylactic vaccine and conventional treatment could suppress HBV, but could not completely eradicate it. The major obstacle for investigating curative antiviral drugs are the incompetence of hepatocyte models that should have closely imitated natural human infection. Here, we demonstrated that an immortalized hepatocyte-like cell line (imHC) could accommodate for over 30 days the entire life cycle of HBV prepared from either established cultured cells or clinically-derived fresh isolates. Normally, imHCs had intact interferon signaling with anti-viral action. Infected imHCs responded to treatments with direct-acting antiviral drugs (DAAs) and interferons (IFNs) by diminishing HBV DNA, the covalently closed circular DNA (cccDNA) surface antigen of HBV (HBsAg, aka the Au...

Journal of Visualized Experiments

Hepatitis B virus (HBV) infection has been considered a crucial risk factor for hepatocellular ca... more Hepatitis B virus (HBV) infection has been considered a crucial risk factor for hepatocellular carcinoma. Current treatment can only lessen the viral load but not result in complete remission. An efficient hepatocyte model for HBV infection would offer a true-to-life viral life cycle that would be crucial for the screening of therapeutic agents. Most available anti-HBV agents target lifecycle stages post viral entry but not before viral entry. This protocol details the generation of a competent hepatocyte model capable of screening for therapeutic agents targeting pre-viral entry and post viral entry lifecycle stages. This includes the targeting of sodium taurocholate cotransporting polypeptide (NTCP) binding, cccDNA formation, transcription, and viral assembly based on imHC or HepaRG as host cells. Here, the HBV entry inhibition assay used curcumin to inhibit HBV binding and transporting functions via NTCP. The inhibitors were evaluated for binding affinity (K D) with NTCP using isothermal titration calorimetry (ITC)-a universal tool for HBV drug screening based on thermodynamic parameters. laden with a risk of liver cirrhosis and hepatocellular carcinoma 1. Current anti-HBV treatment focuses mostly on post viral entry using nucleos(t)ide analogs (NAs) and interferon-alpha (IFN-α) 2 , 3. The discovery of an HBV entry inhibitor, Myrcludex B, has identified a novel target for anti-HBV agents 4. The combination of entry inhibitors and NAs in chronic HBV has significantly lessened the viral load compared to those targeting viral replication Copyright © 2022 JoVE Journal of Visualized Experiments jove.com May 2022 • 183 • e63761 • Page 2 of 17 the receptor via non-covalent interactions of the biomolecular complex 23 , 24. For instance, K D ≥ 1 × 10 3 mM represents weak binding, K D ≥ 1 × 10 6 µM represents moderate binding, and K D ≤ 1 × 10 9 nM represents strong binding. The ΔG is directly correlated with binding interactions. In particular, a reaction with negative ΔG is an exergonic reaction, indicating that binding is a spontaneous process. A reaction with a negative ΔH indicates that the binding processes depend on hydrogen bonding and Van der Waals forces. Both TCA

Allergic contact dermatitis (ACD) is a type IV hypersensitivity mainly mediated by Th1/Th17 immun... more Allergic contact dermatitis (ACD) is a type IV hypersensitivity mainly mediated by Th1/Th17 immune response. Topical corticosteroid is currently the first-line treatment for allergic contact dermatitis (ACD) and systemic administration of immunosuppressive drugs are used in patients with severe disseminated cases. However, increased risk of adverse effects has limited their use. Thus, the development of a novel immunosuppressant for ACD with low toxicity is a challenging issue. In this study, we began our study by using a murine contact hypersensitivity (CHS) model of ACD to examine the immunosuppressive effects of DYRK1B inhibition. We found that mice treated with a selective DYRK1B inhibitor show reduced ear inflammation. In addition, a significant reduction of Th1 and Th17 cells in the regional lymph node upon DYRK1B inhibition was observed by FACS analysis. Studies in vitro further revealed that DYRK1B inhibitor does not only suppressed Th1 and Th17 differentiation, but also pro...

Journal of Medical Case Reports, Aug 4, 2022

Background: The loss of limbal stem cells owing to either corneal burn or inflammation leads to t... more Background: The loss of limbal stem cells owing to either corneal burn or inflammation leads to the repopulation of opaque skin over the raw surface of the cornea. It has been proposed that reconstitution of oral mucosal stem cells over this raw surface will mimic the limbal stem cells and restore vision. The efficacy and safety of applying a sheet of cultivated oral mucosal cells as an autologous graft for corneal replacement were evaluated. Case presentation: The study was conducted during 2014-2015 and involved a total of six patients, of whom three had suffered a chemical burn and three had Stevens-Johnson Syndrome (SJS). Oral mucosal tissue was dissected from each patient, seeded onto irradiated J2 fibroblast feeder cells for 14 days, and analyzed for quality and safety 1 day before being transplanted onto the cornea of the affected eyes. After transplantation, topical antibiotic and antiinflammatory eye drops were instilled four times daily, and the patients wore contact lenses. Subjects were clinically followed for visual acuities and adverse effects at 2, 4, and 6 weeks, 3 and 6 months, and 1 year post-transplantation. Data were presented descriptively. Visual acuities in patients improved at 2 weeks post-surgery. However, two patients with SJS had corneal ulcer at 2 weeks postoperatively. At the 1-year postoperative examination, the eyes of two patients were in good condition with decreased vascularization and epithelial defect. Conclusions: Cultivated oral mucosal epithelial sheet transplantation in limbal stem cell deficiency had a favorable efficacy. In this study, patients with chemical burn had more clinical benefit than those with SJS. Trial registration ClinicalTrials.gov: NCT02415218. Registered retrospectively 4 Apr 2015 (https:// clini caltr ials. gov/ ct2/ show/ NCT02 415218).

Primer sets and conditions used in quantitative real-time PCR (qPCR). (DOCX 15 kb)

The identity of mDCs was demonstrated using flow cytometry analysis for DC markers. The studied m... more The identity of mDCs was demonstrated using flow cytometry analysis for DC markers. The studied markers included CD80, CD83, CD86, CD40 and HLA-DR. (TIF 1013.76 kb)

Immunology, 2019

Cancer research, Mar 22, 2024

Pharmaceutical Sciences Asia, 2022

Journal of Asthma and Allergy, Apr 1, 2023

PubMed, Oct 1, 2016

African Journal of Traditional, Complementary and Alternative Medicines, May 4, 2009

วารสารเภสัชวิทยา (Thai Journal of Pharmacology), 1999

Cancer Research, Apr 4, 2023

Siriraj Medical Journal, May 1, 2022

Cancer research, Mar 22, 2024

Journal of the Medical Association of Thailand = Chotmaihet thangphaet, 2012

Viruses, 2019

Journal of Visualized Experiments

Journal of Medical Case Reports, Aug 4, 2022

Primer sets and conditions used in quantitative real-time PCR (qPCR). (DOCX 15 kb)

A HeLa S3 subline is unusual in accumulating relatively large amounts of glucosaminyl(acyl)phosph... more A HeLa S3 subline is unusual in accumulating relatively large amounts of glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), a derivative of phosphatidylinositol (PI) in which both GlcN and a fatty acid are linked to inositol hydroxyl groups (D. Sevlever, D. Humphrey, and T.L. Rosenberry, submitted for publication). This lipid is a proposed intermediate on the biosynthetic pathway for glycosyl-PI (GPI) anchors of membrane proteins. In this study we demonstrate for the first time that exogenous inositol phospholipids can enter this biosynthetic pathway and be metabolized to GlcN(acyl)PI. When HeLa S3 cells were incubated for 24 h with exogenous PI or sn-1-acyl-2-lyso-phosphatidyl-inositol (lyso-PI) labeled with 3H in the inositol group, 25-30% of the label was recovered in cell-associated lipids and most of the remaining 70-75% in hydrophilic metabolites in the medium. The predominant labeled lipid was PI, with smaller amounts of lyso-PI, phosphatidylinositol 4-phosphate (PIP), and GlcN(acyl)PI. Both exogenous lipid precursors gave the same distribution of labeled lipids, and a similar distribution was observed for endogenous inositol phospholipids metabolically labeled with [3H]inositol. Addition of excess inositol had no effect on the conversion of [3H]lyso-PI to [3H]GlcN(acyl)PI, indicating that the conversion did not result from breakdown to [3H]inositol followed by resynthesis. The cellular orientation of incorporated PI and lyso-PI was determined by incubating cells at 4 degrees C with PI-specific phospholipase C (PI-PLC). This enzyme cleaves only PI and lyso-PI on the outer leaflet of the cell membrane. After 24-h incubation with either precursor, only about 15% of cell-associated [3H]PI or [3H]lyso-PI was on the outer leaflet. However, more than 60% of the [3H]PI was on the outer leaflet after 1-h incubation with either precursor, suggesting that substantial sn-2 acylation of exogenous [3H]lyso-PI occurred in the outer leaflet. This suggestion was confirmed by examining labeled lipids in cells after uptake of [3H]lyso-PI at 4 degrees C. No transmembrane translocation of lyso-PI, PI phosphorylation, or PI glycosylation occurred at this temperature, but some sn-2 acylation was apparent and more than 90% of the [3H]PI formed was on the outer leaflet. These data indicate that sn-2 acylation can occur in the outer leaflet of the cell membrane, perhaps by transacylation from other cell surface phospholipids.

BACKGROUND: Giant cell tumor of bone is a common benign aggressive bone tumor. Recurrent rate in ... more BACKGROUND: Giant cell tumor of bone is a common benign aggressive bone tumor. Recurrent rate in the patients who have the tumor with soft tissue invasion is rather high. Use of strong chemicals such as phenol and liquid nitrogen to lessen the recurrent rate might not be used in this lesion. OBJECTIVE: The experiment was carried out to discover apoptotic effects of thermoablation on giant cell tumor of bone to find out a possibility to use thermoablation in the clinic in the patients with extensive lesion. MATERIAL AND METHOD: Tumor cell suspension was prepared from 4 patients who had definite diagnosis of giant cell tumor of bone. The tumor cells were subjected to thermoablation at 45, 47 and 50 degrees C for 10 to 30 minutes before were cultured at 37 degrees C for another 3 days. Osteoblasts and chondrocytes from the last 3 patients were collected,prepared and underwent thermoablation in the same fashion. Apoptosis of tumor cells, chondrocytes and osteoblasts were carried out by the use of flow cytometry. RESULTS: Thermoablation at 47 degrees C for 30 minutes resulted in < 50% chondrocyte and osteoblast apoptosis and 70-90% tumor cells apoptosis of 3/4 patients. Thermoablation at 47 and 50 degrees C for 20 to 30 minutes has more negative effect on giant cell tumor of bone than chondrocytes and osteoblasts. CONCLUSION: Thermoablation might be a useful tool for local tumor control in the clinic.