mohammad almasi | Malayer University (original) (raw)

Papers by mohammad almasi

Archives of Virology, 2017

An RT-LAMP assay was developed to detect Mirafiori lettuce big vein virus (MiLBVV) and was compar... more An RT-LAMP assay was developed to detect Mirafiori lettuce big vein virus (MiLBVV) and was compared with DAS-ELISA and RT-PCR. All primers were designed on the basis of the coat protein gene of the virus. In addition, a novel immunocapture (IC) RT-LAMP assay for rapid and easy detection of MiLBVV was developed, and factors such as safety, simplicity, cost, user-friendliness and safety were compared with those of DAS-ELISA, RT-PCR and RT-LAMP assays. Compared with DAS-ELISA and RT-PCR, RT-LAMP and IC-RT-LAMP had higher sensitivity (100-fold) but similar specificity, with the advantage of a shorter assay time and no need for RNA extraction (in IC-RT-LAMP). As RT-LAMP requires only very basic instruments and the results can be obtained by visual inspection (using GeneFinder TM dye), this technique provides a simple and reliable tool for laboratory research.

Journal of Plant Pathology & Microbiology, 2012

Loop-mediated isothermal amplification (LAMP) assay is a novel technique for amplifying DNA under... more Loop-mediated isothermal amplification (LAMP) assay is a novel technique for amplifying DNA under constant temperature, with high specificity, sensitivity, rapidity and efficiency. We applied a rapid detection protocol, for the first time, which utilizes colorimetric loop-mediated isothermal amplification for detection of Tomato Yellow Leaf Curl virus. In this regard, all four LAMP primers (i.e. F3, B3, FIP and BIP,) together with PCR primers (F and R) were designed on the basis of the SF gene of the DNA sequences of TYLCV. Even though DAS-ELISA, PCR and LAMP assays could successfully detect positive infected samples, considering the time, safety, sensitivity, cost and simplicity, the last one was overall superior. Meanwhile, among five different visual dyes to accurately detect LAMP products, both hydroxynaphthol blue and GeneFinderTM could produce long stable color change and brightness in a close tube-based approach to prevent cross-contamination risk, concluded eventually as the best ones. All the results, overall, indicated that the LAMP offers an interesting novel and convenient assay format for the rapid, sensitive, cost-effective, and fairly user friendly diagnostic tool of recognition of Tomato Yellow Leaf Curl virus and therefore presents an alternative to PCR-based assays.

Chemical methodologies, Apr 1, 2020

In order to study the nature, type, and magnitude of interactions in the binary mixtures of methy... more In order to study the nature, type, and magnitude of interactions in the binary mixtures of methyl-ethyl-ketone and a series of 2-alkanol from 2propanol up to 2-heptanol, excess (or deficit) numbers of i and j molecules around a central molecule j (Δnij), by being linked to the KB integrals, have been used and reported at T=298.15 K. Negative values of Δnij and Δnji and positive values of Δnii and Δnjj indicate the fact that in the mixtures the tendency of the similar molecular components to form the new interactions and stay together is much higher than the dissimilar molecular components. Investigating the changes in the calculated quantities for different mixtures shows that with the increase in the length of the alcoholic chain, the tendency of the heterogeneous molecules to interact with each other decreases.

Journal of Chemical & Engineering Data, May 17, 2018

In this article the behavior of cyclohexanone + 1-alkanol, namely, 1-pentanol, 1-hexanol, 1-hepta... more In this article the behavior of cyclohexanone + 1-alkanol, namely, 1-pentanol, 1-hexanol, 1-heptanol, 1-octanol, 1-nonanol, and 1-decanol, binary mixtures through the density and viscosity measurements has been studied as a function of composition and within the temperature range of 293.15–323.15 K. The excess molar volume and viscosity deviations have been calculated and fitted by the Redlich–Kister polynomial equation. For binary mixtures of cyclohexanone + 1-pentanol, values of excess molar volume are negative and for other mixtures are positive. For all binary systems viscosity deviations are negative and increase with the alkyl chain of alcohol. The results provide information on the interactions among the molecules in the pure state as well as the binary liquid mixtures. The measured densities and viscosities have been applied to test the applicability of the PC-SAFT and free volume models.

Journal of Solution Chemistry, Nov 8, 2022

Fluid Phase Equilibria, 2020

Isobaric thermal expansibility (p,ρ,T) data of ionic liquid 1-hexyl-3-methylimidazolium bis(trifl... more Isobaric thermal expansibility (p,ρ,T) data of ionic liquid 1-hexyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide [HMIM][NTf 2 ] at T = (273.15 to 413.15) K and pressures up to p = 140 MPa are reported with an estimated experimental relative combined standard uncertainty of Δρ/ρ = ±(0.01 to 0.08) % in density. The measurements are carried out with a newly constructed Anton-Paar DMA HPM vibration-tube densimeter. The system was calibrated using double-distilled water, aqueous NaCl solutions, methanol, toluene and acetone. An empiric equation of state for fitting of the (p,ρ,T) data of [HMIM][NTf 2 ] has been developed as a function of pressure and temperature. This equation is used for the calculation of the thermophysical properties of IL, such as isothermal compressibility, isobaric thermal expansibility, thermal pressure coefficient, internal pressure, isobaric and isochoric heat capacities, speed of sound and isentropic expansibility.

Journal of Solution Chemistry

International Journal of Informatics and Communication Technology (IJ-ICT)

Tracking moving objects has been an issue in recent years of computer vision and image processing... more Tracking moving objects has been an issue in recent years of computer vision and image processing and human tracking makes it a more significant challenge. This category has various aspects and wide applications, such as autonomous deriving, human-robot interactions, and human movement analysis. One of the issues that have always made tracking algorithms difficult is their interaction with goal recognition methods, the mutable appearance of variable aims, and simultaneous tracking of multiple goals. In this paper, a method with high efficiency and higher accuracy was compared to the previous methods for tracking just objects using imaging with the fixed camera is introduced. The proposed algorithm operates in four steps in such a way as to identify a fixed background and remove noise from that. This background is used to subtract from movable objects. After that, while the image is being filtered, the shadows and noises of the filmed image are removed, and finally, using the bubble ...

Journal of the Eastern Asia Society for Transportation Studies, 2015

There is an increasing population rate especially in metropolitan areas and the majority of peopl... more There is an increasing population rate especially in metropolitan areas and the majority of people living in such areas have to spend some part of their time commuting to their destinations. So, one of the serious concerns for network design is defining an efficient and appropriate network being able to shift passenger's mode from private to public transportation properly. The public transport can have an effective role in deriving passenger satisfaction and reducing the operating cost by means of designing a well-integrated public transit system along with improving the cost-effectiveness network. The main goal of this study is to present an improved model for feeder bus network design problem by minimizing total cost. In this study, Genetic Algorithm (GA) and Particle swarm optimization (PSO) were employed to optimize feeder bus services. The case study and input data which have been applied through the current study were already used by Kuha & Perl (1989). Finally, obtained n...

Revista Colombiana de Ciencias Pecuarias, 2019

Background: Prolificacy has a high economic value and is one of the most important reproduction t... more Background: Prolificacy has a high economic value and is one of the most important reproduction traits in small ruminants such as Markhoz goats, which is an endangered breed. Objective: To estimate genetic, environmental and phenotypic trends, and genetic parameters for litter size (LS), as a prolificacy trait of Markhoz goat does. Methods: The study was conducted using kidding records of 3,064 Markhoz does from 1992 to 2015. Genetic parameters were estimated with a logit link function using ASReml software, fitting a repeatability animal model, with birth year, kidding year, and kidding age as fixed effects, and direct additive genetic and permanent environmental effects as random effects. Genetic, phenotypic and environmental trends were estimated by using regression of breeding value, phenotypic value and environmental deviation averages on birth year, respectively. Results: Low and negligible heritability (0.002), repeatability (0.054) and coefficient of permanent environment (0...

Annals of Animal Science, 2020

Weaning traits, including preweaning daily gain (PWDG) and weaning weight (WW) are important econ... more Weaning traits, including preweaning daily gain (PWDG) and weaning weight (WW) are important economic traits, especially for meat type mammals, with high impacts on growth performance and survival rate in higher ages. This study was conducted to perform a genome-wide association study (GWAS) on weaning traits in a meat type breed of sheep. Body weight records of 7557 Lori-Bakhtiari sheep with PWDG and WW records were used to estimate breeding values (EBVs) using an animal mixed model. A total of 132 animals were selected by two-tailed selection strategy, based on EBVs for body weight and then were genotyped using Illumina 50k Ovine SNP chip. After quality control, a total of 130 animals and 41323 SNPs were remained for further analyses. De-regressed estimates of breeding values were used as a pseudo-phenotype in GWAS analysis. Based on Bonferroni-adjusted p-values, five SNPs, located on chromosomes 2, 3, 4, 12 and 22 were significantly (p < 0.05) associated with weaning traits an...

International Journal of Computer Applications, 2019

Face detection in uncontrolled ambiance continues to be a challenge to traditional face detection... more Face detection in uncontrolled ambiance continues to be a challenge to traditional face detection methods due to the large difference in facial expressions. The task of alignment is particularly difficult when the face comes from an extremely unconditional environment. To overcome these problems, the present paper is a reliable and deeply considerable application that allows users to detect a face(s) in real-time and process them. Database of faces with bounding rectangles and facial landmark locations is collected, and simple discriminative classifiers are learned from each of them. Then methods for morphing, warping, swapping and averaging of faces are presented. As a result, faces can be very effectively detected, aligned/ oriented and used for the above methods. In addition, based on results this approach can detect faces and eyes in difficult conditions without explicitly simulating their variation. Evaluating the tests of the application, it can confidently said that this face detection method is accurate and effective and reaches the most modern level of performance. The same methodology can easily be generalized to other tasks, as well as the detection of a common object.

Basic & Clinical Cancer Research, 2019

Background: Research shows that prostate cancer ranks second among the top five most common cance... more Background: Research shows that prostate cancer ranks second among the top five most common cancers in men. It has been confirmed that when circulating Prostate Specific Antigen (PSA) transcripts are successfully detected, prostate cancer cells can be diagnosed at an early stage. A reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) assay was developed and compared to reverse transcriptase polymerase chain reaction (RT-PCR) assay for detection of PSA. Methods: 47 patients, including 30 patients with prostate cancer, 15 with Benign Prostate Hyperplasia (BPH) and 2 healthy subjects as negative controls were included in this study. The prostate cancer cell lines (PC3 and LNCaP) of two patients were included in the study as positive controls. Next, RNA was extracted from fresh samples and a first strand cDNA synthesis kit was applied for the synthesis of cDNA. The human prostate specific antigen gene was used to design specific primers. Results: The results indicated ...

Journal of Chemical & Engineering Data, 2018

In this article the behavior of cyclohexanone + 1-alkanol, namely, 1-pentanol, 1-hexanol, 1-hepta... more In this article the behavior of cyclohexanone + 1-alkanol, namely, 1-pentanol, 1-hexanol, 1-heptanol, 1-octanol, 1-nonanol, and 1decanol, binary mixtures through the density and viscosity measurements has been studied as a function of composition and within the temperature range of 293.15−323.15 K. The excess molar volume and viscosity deviations have been calculated and fitted by the Redlich− Kister polynomial equation. For binary mixtures of cyclohexanone + 1pentanol, values of excess molar volume are negative and for other mixtures are positive. For all binary systems viscosity deviations are negative and increase with the alkyl chain of alcohol. The results provide information on the interactions among the molecules in the pure state as well as the binary liquid mixtures. The measured densities and viscosities have been applied to test the applicability of the PC-SAFT and free volume models.

Cogent Engineering, 2018

In analysis of priority junctions, control delays to minor street automobiles are often approxima... more In analysis of priority junctions, control delays to minor street automobiles are often approximated utilizing the present mathematical models. Nevertheless, the applicability of such a technique depends on the basis and the source of the data with which the model was calibrated. This research was accomplished to establish control delays using Multi-Linear Regression (MLR) for left-and rightturning maneuver from minor road at priority junctions in suburban areas. The data were gathered at two priority junctions utilizing video recording method. In both movements, control delays to minor street automobiles increase as the volume of main street traffic increases. The comparisons among observed control delays and the values estimated utilizing the Malaysia Highway Capacity Manual pointed out that; in general, the observed delays were not in a good agreement with the values computed by theoretical model especially within right turn from minor road. Additionally, the outcomes of this study revealed that the observed delays were in a well agreement with values predicted using the MLR's models for left and right turns. Such a finding signifies that new model is directly applicable to the evaluation of control delays at priority junctions in Malaysia.

American Journal of Infection Control, 2016

Journal of Plant Pathology & Microbiology, 2013

Loop-Mediated Isothermal Amplification (LAMP) assay is a novel technique for amplifying DNA under... more Loop-Mediated Isothermal Amplification (LAMP) assay is a novel technique for amplifying DNA under constant temperature, with high specificity, sensitivity, rapidity and efficiency. To diminish the time required for some diagnostic assays including Reverse Transcription PCR (RT-PCR), Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) and also DAS-ELISA into a minimum level, an innovative colorimetric IC-RT-LAMP and IC-RT-PCR protocol on the basis of Potato Virus Y (PVY) genome were used and optimized. Firstly, DAS-ELISA assay was performed to detect of the virus in a collection containing 95 suspicious samples. Lastly, five samples were detected as the positive samples. Then, the positive samples were verified by molecular methods. In this regard, all four RT-LAMP primers (i.e. F3, B3, FIP and BIP) together with RT-PCR primers (F and B) were selected on the basis of coat protein gene (CP) of PVY genome. Even though DAS-ELISA, RT-PCR and RT-LAMP assays could successfully detect positive infected plant samples, considering the time, safety, sensitivity, cost and simplicity, the last one was overall superior. Furthermore, the results demonstrated that the RT-LAMP assay was 100 times sensitive and 3 time faster compared to RT-PCR. LAMP assay was accomplished in the water bath either frees from any thermal cycler machine or sophisticated laboratories facility. Meanwhile, among six different visual dyes to accurately detect IC-RT-LAMP products, Hydroxynaphthol blue, GeneFinder TM and SYBR Green I could produce long stable colour change and brightness in a close tube-based approach to prevent cross-contamination risk, concluded eventually as the best ones. We accordingly propose this colorimetric assay as a highly reliable alternative viral recognition system regarding PVY recognition and probably other viral-based diseases.

Sustainability, 2016

One of the serious concerns in network design is creating an efficient and appropriate network ca... more One of the serious concerns in network design is creating an efficient and appropriate network capable of efficiently migrating the passenger's mode of transportation from private to public. The main goal of this study is to present an improved model for combining the feeder bus network design system and the railway transit system while minimizing total cost. In this study, the imperialist competitive algorithm (ICA) and the water cycle algorithm (WCA) were employed to optimize feeder bus and railway services. The case study and input data were based on a real transit network in Petaling Jaya, Kuala Lumpur, Malaysia. Numerical results for the proposed model, including the optimal solution, statistical optimization results and the convergence rate, as well as comparisons are discussed in detail.

Journal of Molecular Liquids, 2016

Journal of Plant Pathology & Microbiology, 2012

Loop-mediated isothermal amplification assay amplifies DNA/RNA with high specificity and sensitiv... more Loop-mediated isothermal amplification assay amplifies DNA/RNA with high specificity and sensitivity. In this study, we describe an optimized reverse transcription-LAMP assay for detection of Potato Leafroll Virus. Firstly, DAS-ELISA assay was performed to detect of the virus in a collection containing 40 suspicious samples. Lastly, two samples were detected as the positive samples. Then, the positive samples were verified by RT-PCR and RT-LAMP methods. Furthermore, the results demonstrated that the RT-LAMP assay was 40 times sensitive and 4 time faster compared to RT-PCR. RT-LAMP assay was accomplished in the water bath either frees from any thermal cycler machine or sophisticated laboratories facility. Moreover, in RT-LAMP reaction the positive samples were detected through turbidity which produced by magnesium pyrophosphate. Interestingly, the application of CaCl 2 instead of MgSO 4 which create calcium pyrophosphate in reaction could significantly increase both stability and concentration of turbidity. Consequently, it could be an interesting alternative to MgSO4. Overall, the newly developed RT-LAMP assay can be a sensitive, specific and low-cost method for early detection of Potato Leafroll Virus and also other viral plant pathogens.

Archives of Virology, 2017

An RT-LAMP assay was developed to detect Mirafiori lettuce big vein virus (MiLBVV) and was compar... more An RT-LAMP assay was developed to detect Mirafiori lettuce big vein virus (MiLBVV) and was compared with DAS-ELISA and RT-PCR. All primers were designed on the basis of the coat protein gene of the virus. In addition, a novel immunocapture (IC) RT-LAMP assay for rapid and easy detection of MiLBVV was developed, and factors such as safety, simplicity, cost, user-friendliness and safety were compared with those of DAS-ELISA, RT-PCR and RT-LAMP assays. Compared with DAS-ELISA and RT-PCR, RT-LAMP and IC-RT-LAMP had higher sensitivity (100-fold) but similar specificity, with the advantage of a shorter assay time and no need for RNA extraction (in IC-RT-LAMP). As RT-LAMP requires only very basic instruments and the results can be obtained by visual inspection (using GeneFinder TM dye), this technique provides a simple and reliable tool for laboratory research.

Journal of Plant Pathology & Microbiology, 2012

Loop-mediated isothermal amplification (LAMP) assay is a novel technique for amplifying DNA under... more Loop-mediated isothermal amplification (LAMP) assay is a novel technique for amplifying DNA under constant temperature, with high specificity, sensitivity, rapidity and efficiency. We applied a rapid detection protocol, for the first time, which utilizes colorimetric loop-mediated isothermal amplification for detection of Tomato Yellow Leaf Curl virus. In this regard, all four LAMP primers (i.e. F3, B3, FIP and BIP,) together with PCR primers (F and R) were designed on the basis of the SF gene of the DNA sequences of TYLCV. Even though DAS-ELISA, PCR and LAMP assays could successfully detect positive infected samples, considering the time, safety, sensitivity, cost and simplicity, the last one was overall superior. Meanwhile, among five different visual dyes to accurately detect LAMP products, both hydroxynaphthol blue and GeneFinderTM could produce long stable color change and brightness in a close tube-based approach to prevent cross-contamination risk, concluded eventually as the best ones. All the results, overall, indicated that the LAMP offers an interesting novel and convenient assay format for the rapid, sensitive, cost-effective, and fairly user friendly diagnostic tool of recognition of Tomato Yellow Leaf Curl virus and therefore presents an alternative to PCR-based assays.

Chemical methodologies, Apr 1, 2020

In order to study the nature, type, and magnitude of interactions in the binary mixtures of methy... more In order to study the nature, type, and magnitude of interactions in the binary mixtures of methyl-ethyl-ketone and a series of 2-alkanol from 2propanol up to 2-heptanol, excess (or deficit) numbers of i and j molecules around a central molecule j (Δnij), by being linked to the KB integrals, have been used and reported at T=298.15 K. Negative values of Δnij and Δnji and positive values of Δnii and Δnjj indicate the fact that in the mixtures the tendency of the similar molecular components to form the new interactions and stay together is much higher than the dissimilar molecular components. Investigating the changes in the calculated quantities for different mixtures shows that with the increase in the length of the alcoholic chain, the tendency of the heterogeneous molecules to interact with each other decreases.

Journal of Chemical & Engineering Data, May 17, 2018

In this article the behavior of cyclohexanone + 1-alkanol, namely, 1-pentanol, 1-hexanol, 1-hepta... more In this article the behavior of cyclohexanone + 1-alkanol, namely, 1-pentanol, 1-hexanol, 1-heptanol, 1-octanol, 1-nonanol, and 1-decanol, binary mixtures through the density and viscosity measurements has been studied as a function of composition and within the temperature range of 293.15–323.15 K. The excess molar volume and viscosity deviations have been calculated and fitted by the Redlich–Kister polynomial equation. For binary mixtures of cyclohexanone + 1-pentanol, values of excess molar volume are negative and for other mixtures are positive. For all binary systems viscosity deviations are negative and increase with the alkyl chain of alcohol. The results provide information on the interactions among the molecules in the pure state as well as the binary liquid mixtures. The measured densities and viscosities have been applied to test the applicability of the PC-SAFT and free volume models.

Journal of Solution Chemistry, Nov 8, 2022

Fluid Phase Equilibria, 2020

Isobaric thermal expansibility (p,ρ,T) data of ionic liquid 1-hexyl-3-methylimidazolium bis(trifl... more Isobaric thermal expansibility (p,ρ,T) data of ionic liquid 1-hexyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide [HMIM][NTf 2 ] at T = (273.15 to 413.15) K and pressures up to p = 140 MPa are reported with an estimated experimental relative combined standard uncertainty of Δρ/ρ = ±(0.01 to 0.08) % in density. The measurements are carried out with a newly constructed Anton-Paar DMA HPM vibration-tube densimeter. The system was calibrated using double-distilled water, aqueous NaCl solutions, methanol, toluene and acetone. An empiric equation of state for fitting of the (p,ρ,T) data of [HMIM][NTf 2 ] has been developed as a function of pressure and temperature. This equation is used for the calculation of the thermophysical properties of IL, such as isothermal compressibility, isobaric thermal expansibility, thermal pressure coefficient, internal pressure, isobaric and isochoric heat capacities, speed of sound and isentropic expansibility.

Journal of Solution Chemistry

International Journal of Informatics and Communication Technology (IJ-ICT)

Tracking moving objects has been an issue in recent years of computer vision and image processing... more Tracking moving objects has been an issue in recent years of computer vision and image processing and human tracking makes it a more significant challenge. This category has various aspects and wide applications, such as autonomous deriving, human-robot interactions, and human movement analysis. One of the issues that have always made tracking algorithms difficult is their interaction with goal recognition methods, the mutable appearance of variable aims, and simultaneous tracking of multiple goals. In this paper, a method with high efficiency and higher accuracy was compared to the previous methods for tracking just objects using imaging with the fixed camera is introduced. The proposed algorithm operates in four steps in such a way as to identify a fixed background and remove noise from that. This background is used to subtract from movable objects. After that, while the image is being filtered, the shadows and noises of the filmed image are removed, and finally, using the bubble ...

Journal of the Eastern Asia Society for Transportation Studies, 2015

There is an increasing population rate especially in metropolitan areas and the majority of peopl... more There is an increasing population rate especially in metropolitan areas and the majority of people living in such areas have to spend some part of their time commuting to their destinations. So, one of the serious concerns for network design is defining an efficient and appropriate network being able to shift passenger's mode from private to public transportation properly. The public transport can have an effective role in deriving passenger satisfaction and reducing the operating cost by means of designing a well-integrated public transit system along with improving the cost-effectiveness network. The main goal of this study is to present an improved model for feeder bus network design problem by minimizing total cost. In this study, Genetic Algorithm (GA) and Particle swarm optimization (PSO) were employed to optimize feeder bus services. The case study and input data which have been applied through the current study were already used by Kuha & Perl (1989). Finally, obtained n...

Revista Colombiana de Ciencias Pecuarias, 2019

Background: Prolificacy has a high economic value and is one of the most important reproduction t... more Background: Prolificacy has a high economic value and is one of the most important reproduction traits in small ruminants such as Markhoz goats, which is an endangered breed. Objective: To estimate genetic, environmental and phenotypic trends, and genetic parameters for litter size (LS), as a prolificacy trait of Markhoz goat does. Methods: The study was conducted using kidding records of 3,064 Markhoz does from 1992 to 2015. Genetic parameters were estimated with a logit link function using ASReml software, fitting a repeatability animal model, with birth year, kidding year, and kidding age as fixed effects, and direct additive genetic and permanent environmental effects as random effects. Genetic, phenotypic and environmental trends were estimated by using regression of breeding value, phenotypic value and environmental deviation averages on birth year, respectively. Results: Low and negligible heritability (0.002), repeatability (0.054) and coefficient of permanent environment (0...

Annals of Animal Science, 2020

Weaning traits, including preweaning daily gain (PWDG) and weaning weight (WW) are important econ... more Weaning traits, including preweaning daily gain (PWDG) and weaning weight (WW) are important economic traits, especially for meat type mammals, with high impacts on growth performance and survival rate in higher ages. This study was conducted to perform a genome-wide association study (GWAS) on weaning traits in a meat type breed of sheep. Body weight records of 7557 Lori-Bakhtiari sheep with PWDG and WW records were used to estimate breeding values (EBVs) using an animal mixed model. A total of 132 animals were selected by two-tailed selection strategy, based on EBVs for body weight and then were genotyped using Illumina 50k Ovine SNP chip. After quality control, a total of 130 animals and 41323 SNPs were remained for further analyses. De-regressed estimates of breeding values were used as a pseudo-phenotype in GWAS analysis. Based on Bonferroni-adjusted p-values, five SNPs, located on chromosomes 2, 3, 4, 12 and 22 were significantly (p < 0.05) associated with weaning traits an...

International Journal of Computer Applications, 2019

Face detection in uncontrolled ambiance continues to be a challenge to traditional face detection... more Face detection in uncontrolled ambiance continues to be a challenge to traditional face detection methods due to the large difference in facial expressions. The task of alignment is particularly difficult when the face comes from an extremely unconditional environment. To overcome these problems, the present paper is a reliable and deeply considerable application that allows users to detect a face(s) in real-time and process them. Database of faces with bounding rectangles and facial landmark locations is collected, and simple discriminative classifiers are learned from each of them. Then methods for morphing, warping, swapping and averaging of faces are presented. As a result, faces can be very effectively detected, aligned/ oriented and used for the above methods. In addition, based on results this approach can detect faces and eyes in difficult conditions without explicitly simulating their variation. Evaluating the tests of the application, it can confidently said that this face detection method is accurate and effective and reaches the most modern level of performance. The same methodology can easily be generalized to other tasks, as well as the detection of a common object.

Basic & Clinical Cancer Research, 2019

Background: Research shows that prostate cancer ranks second among the top five most common cance... more Background: Research shows that prostate cancer ranks second among the top five most common cancers in men. It has been confirmed that when circulating Prostate Specific Antigen (PSA) transcripts are successfully detected, prostate cancer cells can be diagnosed at an early stage. A reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) assay was developed and compared to reverse transcriptase polymerase chain reaction (RT-PCR) assay for detection of PSA. Methods: 47 patients, including 30 patients with prostate cancer, 15 with Benign Prostate Hyperplasia (BPH) and 2 healthy subjects as negative controls were included in this study. The prostate cancer cell lines (PC3 and LNCaP) of two patients were included in the study as positive controls. Next, RNA was extracted from fresh samples and a first strand cDNA synthesis kit was applied for the synthesis of cDNA. The human prostate specific antigen gene was used to design specific primers. Results: The results indicated ...

Journal of Chemical & Engineering Data, 2018

In this article the behavior of cyclohexanone + 1-alkanol, namely, 1-pentanol, 1-hexanol, 1-hepta... more In this article the behavior of cyclohexanone + 1-alkanol, namely, 1-pentanol, 1-hexanol, 1-heptanol, 1-octanol, 1-nonanol, and 1decanol, binary mixtures through the density and viscosity measurements has been studied as a function of composition and within the temperature range of 293.15−323.15 K. The excess molar volume and viscosity deviations have been calculated and fitted by the Redlich− Kister polynomial equation. For binary mixtures of cyclohexanone + 1pentanol, values of excess molar volume are negative and for other mixtures are positive. For all binary systems viscosity deviations are negative and increase with the alkyl chain of alcohol. The results provide information on the interactions among the molecules in the pure state as well as the binary liquid mixtures. The measured densities and viscosities have been applied to test the applicability of the PC-SAFT and free volume models.

Cogent Engineering, 2018

In analysis of priority junctions, control delays to minor street automobiles are often approxima... more In analysis of priority junctions, control delays to minor street automobiles are often approximated utilizing the present mathematical models. Nevertheless, the applicability of such a technique depends on the basis and the source of the data with which the model was calibrated. This research was accomplished to establish control delays using Multi-Linear Regression (MLR) for left-and rightturning maneuver from minor road at priority junctions in suburban areas. The data were gathered at two priority junctions utilizing video recording method. In both movements, control delays to minor street automobiles increase as the volume of main street traffic increases. The comparisons among observed control delays and the values estimated utilizing the Malaysia Highway Capacity Manual pointed out that; in general, the observed delays were not in a good agreement with the values computed by theoretical model especially within right turn from minor road. Additionally, the outcomes of this study revealed that the observed delays were in a well agreement with values predicted using the MLR's models for left and right turns. Such a finding signifies that new model is directly applicable to the evaluation of control delays at priority junctions in Malaysia.

American Journal of Infection Control, 2016

Journal of Plant Pathology & Microbiology, 2013

Loop-Mediated Isothermal Amplification (LAMP) assay is a novel technique for amplifying DNA under... more Loop-Mediated Isothermal Amplification (LAMP) assay is a novel technique for amplifying DNA under constant temperature, with high specificity, sensitivity, rapidity and efficiency. To diminish the time required for some diagnostic assays including Reverse Transcription PCR (RT-PCR), Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) and also DAS-ELISA into a minimum level, an innovative colorimetric IC-RT-LAMP and IC-RT-PCR protocol on the basis of Potato Virus Y (PVY) genome were used and optimized. Firstly, DAS-ELISA assay was performed to detect of the virus in a collection containing 95 suspicious samples. Lastly, five samples were detected as the positive samples. Then, the positive samples were verified by molecular methods. In this regard, all four RT-LAMP primers (i.e. F3, B3, FIP and BIP) together with RT-PCR primers (F and B) were selected on the basis of coat protein gene (CP) of PVY genome. Even though DAS-ELISA, RT-PCR and RT-LAMP assays could successfully detect positive infected plant samples, considering the time, safety, sensitivity, cost and simplicity, the last one was overall superior. Furthermore, the results demonstrated that the RT-LAMP assay was 100 times sensitive and 3 time faster compared to RT-PCR. LAMP assay was accomplished in the water bath either frees from any thermal cycler machine or sophisticated laboratories facility. Meanwhile, among six different visual dyes to accurately detect IC-RT-LAMP products, Hydroxynaphthol blue, GeneFinder TM and SYBR Green I could produce long stable colour change and brightness in a close tube-based approach to prevent cross-contamination risk, concluded eventually as the best ones. We accordingly propose this colorimetric assay as a highly reliable alternative viral recognition system regarding PVY recognition and probably other viral-based diseases.

Sustainability, 2016

One of the serious concerns in network design is creating an efficient and appropriate network ca... more One of the serious concerns in network design is creating an efficient and appropriate network capable of efficiently migrating the passenger's mode of transportation from private to public. The main goal of this study is to present an improved model for combining the feeder bus network design system and the railway transit system while minimizing total cost. In this study, the imperialist competitive algorithm (ICA) and the water cycle algorithm (WCA) were employed to optimize feeder bus and railway services. The case study and input data were based on a real transit network in Petaling Jaya, Kuala Lumpur, Malaysia. Numerical results for the proposed model, including the optimal solution, statistical optimization results and the convergence rate, as well as comparisons are discussed in detail.

Journal of Molecular Liquids, 2016

Journal of Plant Pathology & Microbiology, 2012

Loop-mediated isothermal amplification assay amplifies DNA/RNA with high specificity and sensitiv... more Loop-mediated isothermal amplification assay amplifies DNA/RNA with high specificity and sensitivity. In this study, we describe an optimized reverse transcription-LAMP assay for detection of Potato Leafroll Virus. Firstly, DAS-ELISA assay was performed to detect of the virus in a collection containing 40 suspicious samples. Lastly, two samples were detected as the positive samples. Then, the positive samples were verified by RT-PCR and RT-LAMP methods. Furthermore, the results demonstrated that the RT-LAMP assay was 40 times sensitive and 4 time faster compared to RT-PCR. RT-LAMP assay was accomplished in the water bath either frees from any thermal cycler machine or sophisticated laboratories facility. Moreover, in RT-LAMP reaction the positive samples were detected through turbidity which produced by magnesium pyrophosphate. Interestingly, the application of CaCl 2 instead of MgSO 4 which create calcium pyrophosphate in reaction could significantly increase both stability and concentration of turbidity. Consequently, it could be an interesting alternative to MgSO4. Overall, the newly developed RT-LAMP assay can be a sensitive, specific and low-cost method for early detection of Potato Leafroll Virus and also other viral plant pathogens.