Ehsan Ghayoor | The University of Manchester (original) (raw)

Papers by Ehsan Ghayoor

Research paper thumbnail of Respiratory symptoms and pulmonary function changes among carpet weavers in Iran

International journal of occupational and environmental health

Respiratory and allergic symptoms were evaluated in 66 Iranian carpet weavers and 66 controls wit... more Respiratory and allergic symptoms were evaluated in 66 Iranian carpet weavers and 66 controls with similar age and gender characteristics using a questionnaire including questions on work-related respiratory symptoms in the past year, allergies, smoking habits, and work exposure duration. A total of 28 carpet weavers (42%) reported work-related respiratory symptoms. Incidences of allrespiratory symptoms and most allergic symptoms were significantly higher in carpet weavers than in controls (p<0.05 - p<0.001). Moreover, most respiratory and allergic symptoms in carpet weavers were significantly more prominent during working hours (p<0.01 - p<0.001). Pulmonary function test results of the carpet weavers showed significant impairment compared with controls (p<0.05 - p<0.001).

Research paper thumbnail of Mathematical modelling of miRNA mediated BCR.ABL protein regulation in chronic myeloid leukaemia vis-a-vis therapeutic strategies

Integrative biology : quantitative biosciences from nano to macro, 2013

Chronic myeloid leukaemia (CML) is a clonal myeloproliferative disease resulting from an aberrant... more Chronic myeloid leukaemia (CML) is a clonal myeloproliferative disease resulting from an aberrant BCR.ABL gene and protein. To predict BCR.ABL protein abundance and phosphorylation in individual cells in a population of CML cells, we modelled BCR.ABL protein regulation through associated miRNAs using a systems approach. The model rationalizes the level of BCR.ABL protein heterogeneity in CML cells in correlation with the heterogeneous BCR.ABL mRNA levels. We also measured BCR.ABL mRNA and BCR.ABLp phosphorylation in individual cells. The experimental data were consistent with the modelling results, thereby partly validating the model. Provided it is tested further, the model may be used to support effective therapeutic strategies including the combined application of a tyrosine kinase inhibitor and miRNAs targeting BCR.ABL. It appears able to predict different effects of the two types of drug on cells with different expression levels and consequently different effects on the generation of resistance.

Research paper thumbnail of Personalised treatment of haematological malignancies through systems medicine based on single molecules in single cells

Integrative biology : quantitative biosciences from nano to macro, 2013

Molecular diagnostics in haematological malignancies continues to advance towards more personaliz... more Molecular diagnostics in haematological malignancies continues to advance towards more personalized treatment and accordingly demand is increasing for procedures providing quantitative analyses of heterogeneous tissue in malignancies. Circulating leukaemic cells are diverse and comprise discrete clonal populations arising from a common progenitor cell. Some of the current diagnostic techniques possess an attenuated dynamic quantitative range that prevents a clear comprehension of intercellular interactions. Quantitative measurements will facilitate an accurate appreciation of holistic cellular processes, assist with predictions pertaining to perturbations and reveal functional moieties that are truly a facet of the disease, and thus add to current biomarker discovery which often lack assessment of functional involvement in disease mechanisms and processes. This review focuses on quantitative studies related to peripheral blood and haematological malignancies. Data retrieval for either of these diseases is hampered by the high and unchartered degree of heterogeneity typically existing within clinical samples. The likelihood of analysis across single cell populations is highly probable in the near future. This will allow a patient to be readily screened for malignancies and assigned to a risk group based on a quantitative profile of a complex of molecules related to disease. The future analysis of molecular pathology based on detailed molecular dissection looks promising, requiring the integration of various disciplines encompassing morphology, genetics, expression profiling and new and evolving predictive modeling via systems medicine. From this detailed view into patient health, an increasingly personalized treatment plan can be administered, commencing with stratified medicine.

Research paper thumbnail of Expression of IL-17 and COX2 Gene in Peripheral Blood Leukocytes of Vitiligo Patients

Iranian Journal of …, Jan 1, 2011

Vitiligo is a pigmentation disorder in which inflammatory mediators such as cytokines have a pivo... more Vitiligo is a pigmentation disorder in which inflammatory mediators such as cytokines have a pivotal role in disease's pathogenesis. Interleukin 17 (IL-17A) is a proinflammatory cytokine which is involved in the induction of several proinflamatory mediators such as cyclooxygenase 2 (COX2). The aim of this study was to investigate the gene expression of IL-17 and COX2 in peripheral blood leukocytes of vitiligo's patients.

Research paper thumbnail of Association of the Expression of IL-4 and IL-13 Genes, IL-4 and IgE Serum Levels with Allergic Asthma

Iranian Journal of …, Jan 1, 2007

Immune and inflammatory responses mediated by cytokines, play important roles in the pathophysiol... more Immune and inflammatory responses mediated by cytokines, play important roles in the pathophysiology of asthma. These responses are associated with overexpression of Th2 cytokines such as IL-4 and IL-13. These two cytokines use common receptors for signaling that lead to identical immunological effects and regulation of the Th1/Th2 balance. The aim of this study was to determine whether patients with allergic asthma display overexpression of IL-4 and IL-13 genes.

Research paper thumbnail of Respiratory symptoms and pulmonary function changes among carpet weavers in Iran

International journal of occupational and environmental health

Respiratory and allergic symptoms were evaluated in 66 Iranian carpet weavers and 66 controls wit... more Respiratory and allergic symptoms were evaluated in 66 Iranian carpet weavers and 66 controls with similar age and gender characteristics using a questionnaire including questions on work-related respiratory symptoms in the past year, allergies, smoking habits, and work exposure duration. A total of 28 carpet weavers (42%) reported work-related respiratory symptoms. Incidences of allrespiratory symptoms and most allergic symptoms were significantly higher in carpet weavers than in controls (p<0.05 - p<0.001). Moreover, most respiratory and allergic symptoms in carpet weavers were significantly more prominent during working hours (p<0.01 - p<0.001). Pulmonary function test results of the carpet weavers showed significant impairment compared with controls (p<0.05 - p<0.001).

Research paper thumbnail of Mathematical modelling of miRNA mediated BCR.ABL protein regulation in chronic myeloid leukaemia vis-a-vis therapeutic strategies

Integrative biology : quantitative biosciences from nano to macro, 2013

Chronic myeloid leukaemia (CML) is a clonal myeloproliferative disease resulting from an aberrant... more Chronic myeloid leukaemia (CML) is a clonal myeloproliferative disease resulting from an aberrant BCR.ABL gene and protein. To predict BCR.ABL protein abundance and phosphorylation in individual cells in a population of CML cells, we modelled BCR.ABL protein regulation through associated miRNAs using a systems approach. The model rationalizes the level of BCR.ABL protein heterogeneity in CML cells in correlation with the heterogeneous BCR.ABL mRNA levels. We also measured BCR.ABL mRNA and BCR.ABLp phosphorylation in individual cells. The experimental data were consistent with the modelling results, thereby partly validating the model. Provided it is tested further, the model may be used to support effective therapeutic strategies including the combined application of a tyrosine kinase inhibitor and miRNAs targeting BCR.ABL. It appears able to predict different effects of the two types of drug on cells with different expression levels and consequently different effects on the generation of resistance.

Research paper thumbnail of Personalised treatment of haematological malignancies through systems medicine based on single molecules in single cells

Integrative biology : quantitative biosciences from nano to macro, 2013

Molecular diagnostics in haematological malignancies continues to advance towards more personaliz... more Molecular diagnostics in haematological malignancies continues to advance towards more personalized treatment and accordingly demand is increasing for procedures providing quantitative analyses of heterogeneous tissue in malignancies. Circulating leukaemic cells are diverse and comprise discrete clonal populations arising from a common progenitor cell. Some of the current diagnostic techniques possess an attenuated dynamic quantitative range that prevents a clear comprehension of intercellular interactions. Quantitative measurements will facilitate an accurate appreciation of holistic cellular processes, assist with predictions pertaining to perturbations and reveal functional moieties that are truly a facet of the disease, and thus add to current biomarker discovery which often lack assessment of functional involvement in disease mechanisms and processes. This review focuses on quantitative studies related to peripheral blood and haematological malignancies. Data retrieval for either of these diseases is hampered by the high and unchartered degree of heterogeneity typically existing within clinical samples. The likelihood of analysis across single cell populations is highly probable in the near future. This will allow a patient to be readily screened for malignancies and assigned to a risk group based on a quantitative profile of a complex of molecules related to disease. The future analysis of molecular pathology based on detailed molecular dissection looks promising, requiring the integration of various disciplines encompassing morphology, genetics, expression profiling and new and evolving predictive modeling via systems medicine. From this detailed view into patient health, an increasingly personalized treatment plan can be administered, commencing with stratified medicine.

Research paper thumbnail of Expression of IL-17 and COX2 Gene in Peripheral Blood Leukocytes of Vitiligo Patients

Iranian Journal of …, Jan 1, 2011

Vitiligo is a pigmentation disorder in which inflammatory mediators such as cytokines have a pivo... more Vitiligo is a pigmentation disorder in which inflammatory mediators such as cytokines have a pivotal role in disease's pathogenesis. Interleukin 17 (IL-17A) is a proinflammatory cytokine which is involved in the induction of several proinflamatory mediators such as cyclooxygenase 2 (COX2). The aim of this study was to investigate the gene expression of IL-17 and COX2 in peripheral blood leukocytes of vitiligo's patients.

Research paper thumbnail of Association of the Expression of IL-4 and IL-13 Genes, IL-4 and IgE Serum Levels with Allergic Asthma

Iranian Journal of …, Jan 1, 2007

Immune and inflammatory responses mediated by cytokines, play important roles in the pathophysiol... more Immune and inflammatory responses mediated by cytokines, play important roles in the pathophysiology of asthma. These responses are associated with overexpression of Th2 cytokines such as IL-4 and IL-13. These two cytokines use common receptors for signaling that lead to identical immunological effects and regulation of the Th1/Th2 balance. The aim of this study was to determine whether patients with allergic asthma display overexpression of IL-4 and IL-13 genes.