chiquita prahasanti s | Airlangga University (original) (raw)

Papers by chiquita prahasanti s

Journal of oral biology and craniofacial research, Mar 1, 2023

Excessive gingival pigmentation is a major aesthetic concern in modern society, though it is not ... more Excessive gingival pigmentation is a major aesthetic concern in modern society, though it is not a medical problem they consider it as a negative attribute. Patients with gingival hyperpigmentation usually complain and request cosmetic therapy, particularly if the pigmentation is visible during speaking and smiling. Various depigmentation methods, including burr abrasion, cryosurgery, electro-surgery, split thickness flap excision and surgical scraping techniques have been used with varying degrees of success. Recently, lasers have been used to ablate cells containing and producing the melanin pigment. The present study was undertaken to compare the clinical effectiveness and patient comfort of surgical scrapping and diode laser technique used for gingival depigmentation for a follow up period of 6 months.20 subjects participated in this split mouth study. The clinical evaluation parameters included Extent and Intensity of gingival hyperpigmentation, post-operative gingival bleeding and pain. On follow up examination at 6 th month there was no statistical difference in repigmentation extent and intensity between diode laser and surgical scraping techniques. The mean pain scores for treated sites with diode laser were significantly lower than surgical scrapping technique at 24 hours (t-value=2.430, p-value=0.02). The postoperative gingival bleeding at end of procedure was significantly lower with diode laser than surgical scrapping technique (p-value=<0.0001). There was no statistical difference in postoperative re-pigmentation and clinical efficacy among the subjects between surgical scraping and diode laser technique at 6 th month follow up. Diode laser technique provides better haemostasis and good visibility at the surgical site. The post-operative patient comfort is better at the surgical sites treated with diode laser than surgical scrapping method. Hence, both the techniques are used for depigmentation procedures depending on the severity and gingival biotype and patient acceptance.

European Journal of Dentistry, Jun 19, 2023

Objectives Stem cell from human exfoliated deciduous teeth (SHED) has great potential for bone ti... more Objectives Stem cell from human exfoliated deciduous teeth (SHED) has great potential for bone tissue engineering and cell therapy for regenerative medicine. It has been combined with biomaterials such as mixed of polymethylmethacrylate (PMMA) and hydroxyapatite (HA) as candidates for synthetic bone graft biomaterial. The aim of this study was to analyze the toxicity test of mixed PMMA-HA scaffold seeded with SHED and osteoblast in vitro. Materials and Methods SHED was isolated from the pulp of noncarious deciduous teeth and osteoblast cells were cultured, and exposed to PMMA-HA scaffolds with three concentration groups: 20/80, 30/70, and 40/60 for 24 hours. Cytotoxicity test was performed by MTT assay to cell viability. Statistical Analysis Data were analyzed using IBM SPSS Statistics 25, one-way analysis of variance followed by least significant difference test, considering the level of significance p-value less than 0.05 Results The percentage of SHED's viability was best in the PMMA-HA group with concentrations of 20/80, followed by 30/70, and 40/60 with 87.03, 75.33, and 65.79%, respectively. The percentage of osteoblast cell's viability was best in the PMMA-HA group with concentrations of 20/80, followed by 30/70, and 40/60 with 123.6, 108.36, and 93.48%, respectively. Conclusions Mixed PMMA-HA was not toxic for the SHED and osteoblast. This characteristic is the initial requirement to be proposed as an alternative material for healing alveolar bone defects. In vivo animal research is mandatory to confirm the use of PMMA-HA on the alveolar defect model.

Journal of oral biology and craniofacial research, Mar 1, 2023

Nucleation and Atmospheric Aerosols, 2020

Research Journal of Pharmacy and Technology, Jan 27, 2023

Background: Bone graft materials are widely used to support the success of periodontal tissue tre... more Background: Bone graft materials are widely used to support the success of periodontal tissue treatments. Graft material has been used extensively however it has some deficiencies such as limited availability, high levels of resorption, immunological reactions and the risk of contamination as well as high costs. Therefore recent research is needed to explore potential usage of the tooth graft. Objective(s): The objective of this research is to comparing the MMP-13 expression in bone healing after grafting the incisor’s extraction sockets with hydroxyapatite tooth graft and hydroxyapatite xenograft. Research Methods: 33 adult male Cavia cobayas were divided into three groups: hydroxyapatite xenograft, hydroxyapatite tooth graft, and control (without treatment). Bone tissue sections were stained with diaminobenzidine and then dripping with anti-MMP-13 monoclonal antibody. The number of MMP-13 expression by osteoblast cells was carried out quantitatively based on the colourintensity in each group under the OLYMPUS microscope with 400x magnification. Results: The value of MMP-13 expression was higher on the tooth graft and xenograft group than the control group. The ability of osteoconduction of hydroxyapatite derived from porous hydroxyapatite structure will facilitate angiogenesis so that osteogenic cells can migrate and attach to the graft to induce the new bone growth. The tooth graft group had higher MMP-13 value than xenograft group. High solubility of graft material could affect bone remodeling and mineralization processes because of the presence of inflammatory cells is detrimental to the healing process. Conclusion: As a new graft material, tooth graft has a capability to induce osteoconductive and osteoinductive that similar with the xenograft.

Background: Chitosan is a natural polymer with favourable properties such as biocompatibility, bi... more Background: Chitosan is a natural polymer with favourable properties such as biocompatibility, biodegradability, non- allergenic, and non- toxic which make it a very promising material for scaffold in tissue engineering. Application of chitosan based systems in hard tissue engineering such as bone, cartilage, and periodontal are reviewd with recent and relevant examples. Hydroxiapatite is a polymer of calcium phosphates from bones and can be extracted cortical bovine bone. Hydroxiapatite has poor solubility, so it is combined with chitosan because chitosan has high solubility to make good strength scaffold for bone healing.. Purpose: The purpose of this laboratory research is to study the density of collagen on bone with the application of hydroxyapatit chitosan and hydroxyapatite. Methods:. The samples used were 24 healthy 3 months old male wistar’ s rats, with approximately 200 grams of body weight and they were devided into 4 groups. Two groups were taken for hydroxiapatite chitosan and the others for hydroxiapatite. A group were taken for hydroxiapatite chitosan was given for 7 days in group C7, C14 for 14 days. A group were taken for hydroxyapatite was given for 7 days in group H7, H14 for 14 days. Seven days of operation time was waiting before the sample being decapited for further histological examination in the post operation. A hematoxillin staining was used and the density of collagen counted under light microscopy with 400 times magnification T- test and least significant difference test were used for the statistic evaluation. Result:. shown increase of the density of collagen in hydroxiapatite chitosan group of 7 days and the hydroxiapatite chitosan of 14 days is decrease. On the other hand, the result of study shown decrease of the density of collagen in hydroxiapatite group of 14 days and it increase for the group of 7 days. This study that duration time of hydroxiapatite and chitosan had not been interfered significantly with the density of collagen during bone healing after the defect in wistar’s rats. Conclusion: There is no different between hydroxyapatite and hydroxyapatite chitosan application to the density of collagen. Chitosan only can accelerate the bone healing in proliferation phase. Key words: Hydroxyapatite, chitosan, collagen.

THE 2ND INTERNATIONAL CONFERENCE ON PHYSICAL INSTRUMENTATION AND ADVANCED MATERIALS 2019

THE 2ND INTERNATIONAL CONFERENCE ON PHYSICAL INSTRUMENTATION AND ADVANCED MATERIALS 2019, 2020

In the case of tooth loss ideally it is replaced with a dental implant, because dental impants ha... more In the case of tooth loss ideally it is replaced with a dental implant, because dental impants have several advantages such as restoring the function of mastication, increasing mobility and reducing pain. Nowadays after tooth extraction, dental implants are often implanted immediately, with the consideration of preventing mechanical loading, thus obtaining the stability needed for osseointergation of the implant. Titanium (Ti) implants are the most commonly used materials because they have good mechanical properties, biocompability, stable chemical, low toxicity, bioactivity and corrosion resistance. Various implant surface modification materials were carried out to improve implant integration, including hydroxyapatite (HA), antibacterial and BMP-2. These coatings to improve osseointegration and avoid implant infection, thus increasing the success rate of the implant.

Background. Periodontal disease happens approximately at 10% of adult populations, characterized ... more Background. Periodontal disease happens approximately at 10% of adult populations, characterized by loss of attachment beetwen tooth and bone, resulting in bone resorbtion and eventually bone loss. In the wound healing process to repair bone tissue, macrophage have an important role. To facilitate the healing process, bone graft is placed in the bone defect. Compounding hydroxyapatite-chitosan can result in better osteoconductivity properties and scaffold strength than both of material stands alone. Purpose. The aim of this study was to know the effect of hydroxyapatite-chitosan aplication on macrophage proliferation in bone remodelling. Method. This research was done in vivo experiment by created a defect on rabbit’s tibiae. 20 rabbits were divide into 4 groups The 1st group treated with 70:30 hydroxyapatite -chitosan powder, examined after 4 days. 2nd treated with 30:70 hydroxyapatite -chitosan powder, examined after 4 days, 3th group treated with 70:30 hydroxyapatite-chitosan pow...

Nigerian Journal of Clinical Practice, 2021

Background: Periodontal regeneration is the anticipated result of periodontal tissue therapy. Of ... more Background: Periodontal regeneration is the anticipated result of periodontal tissue therapy. Of the many scaffold materials utilized, collagen has been shown to possess characteristics that strongly promote the periodontal regeneration process. Gourami (Osphronemusgoramy) scales can be utilized as an alternative source of collagen due to their abundant availability, the absence of risk of zoonosis, and lack of religious strictures against their use. Aims: To analyze the characteristics of collagen extracted from gourami scales. Methods and Materials: A combination of scanning electron microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR), and in vitro enzymatic biodegradation analysis were employed to determine pore sizes, constituent functional groups, and the degradation rate of collagen extractedfrom gourami scales. Results: Collagen extracted from gourami scales demonstrated an average porosity of 13.779 μm. The FTIR test results indicated Amide A, Amide B, Amide I, ...

Dental Journal (Majalah Kedokteran Gigi)

Background: Periodontitis is a chronic inflammatory disease of the periodontal tissue that is cha... more Background: Periodontitis is a chronic inflammatory disease of the periodontal tissue that is characterized by alveolar bone resorption. This occurs due to an imbalance of osteoblast and osteoclast during the bone formation and resorption processes. In order to obtain complete regeneration of periodontal tissue, bone grafting is frequently used in periodontal surgical therapy. Although each material has disadvantages, safe graft materials derived from animal sources can be employed as an alternative to bone graft materials. Osteoblast, osteoclast, calcified bone matrix, type I collagen, osteonectin, and hydroxyapatite can all be found in gourami scales, a form of food waste. BMP4 has osteoinduction functions, which are important in bone metabolism. Through angiogenic activity, FGF2 also contributes to periodontal regeneration. Purpose: The aim of the study was to assess the expression of BMP4 and FGF2 after the treatment group had been given gourami fish scale extract. Methods: Thir...

Global Journal of Health Science, 2016

Excessive gingival pigmentation is a major aesthetic concern in modern society, though it is not ... more Excessive gingival pigmentation is a major aesthetic concern in modern society, though it is not a medical problem they consider it as a negative attribute. Patients with gingival hyperpigmentation usually complain and request cosmetic therapy, particularly if the pigmentation is visible during speaking and smiling. Various depigmentation methods, including burr abrasion, cryosurgery, electro-surgery, split thickness flap excision and surgical scraping techniques have been used with varying degrees of success. Recently, lasers have been used to ablate cells containing and producing the melanin pigment. The present study was undertaken to compare the clinical effectiveness and patient comfort of surgical scrapping and diode laser technique used for gingival depigmentation for a follow up period of 6 months.20 subjects participated in this split mouth study. The clinical evaluation parameters included Extent and Intensity of gingival hyperpigmentation, post-operative gingival bleeding...

Journal of Pharmacy & Pharmacognosy Research

Context: The most common biomaterial used for dental implants is titanium. However, the release o... more Context: The most common biomaterial used for dental implants is titanium. However, the release of metal ions and the risk of allergic reactions to metals that may occur in some patients cannot be avoided. Hydroxyapatite-polymethylmethacrylate (HA-PMMA) composite biomaterials are proposed to have potential as dental implant biomaterials due to their mechanical, chemical, and biological properties. HA-PMMA may induce osseointegration, biocompatible, less allergic reactions, and no metal ions released. In addition, HA-PMMA can be obtained from Indonesia’s abundant natural resources. Aims: To explore HA-PMMA composites through molecular docking as a biomaterial candidate for dental implants in silico. Methods: Structure data format (sdf), molecular weight, and identity number (CID) of HA-PMMA ligand samples were obtained from PubChem database and minimized through OpenBabel. 3D structure, selection method, resolution, atom count, weight, sequence length, and ID protein BMP2, BMP4, BMP7...

Research journal of pharmacy and technology, Aug 30, 2022

Research Journal of Pharmacy and Technology

Background and Aim: The current goal of periodontal therapy is to achieve periodontal regeneratio... more Background and Aim: The current goal of periodontal therapy is to achieve periodontal regeneration. Important factor for periodontal regeneration is to promote bone formation, nowadays used bone replacement. The used of xenograft materials as gold standard for periodontal tissue regeneration using type I collagen bone graft has been widely developed. The main organic component in fish scales is type I fibril collagen, which are same as with component in bones. Specific markers of bone formation are the expression of osteoblast, osteoclast, osteoprotegerin (OPG), and receptor activator of nuclear factor κB ligands (RANKL). The purpose of this study is to assess the expression of OPG and RANKL after application of extract collagen from gourami (Osphronemus gouramy) scales. Materials and Methods: Thirty-two experimental 3-month-old male Wistar albino rats (150g and 200g) were randomly divided into four groups: 7-day control group, 7-day fish collagen group, 14-day control group and 14-...

International Journal of Integrated Engineering

Hydroxyapatite-tricalcium phosphate (HA-TCP) scaffold is a three-dimensional structure used to su... more Hydroxyapatite-tricalcium phosphate (HA-TCP) scaffold is a three-dimensional structure used to support bone regeneration. Ideally, the scaffold should be biocompatible, biodegradable, and non-toxic. The tissue engineering technique uses a combined stem cell and scaffold to repair the bone defect. To prove the non-toxic properties of the scaffold, a cytotoxicity test is needed for the human umbilical cord mesenchymal stem cell (HUCMSCs). In this study, 27 samples were divided into eight groups with a variant of scaffold HA-TCP doses ranging from 5–1000 µg. Each treatment group of scaffolds was covered in HUCMSCs. The samples were added by methyl-thiazol-tetrazolium (MTT) colorimetric assay, counted using an optical density (OD) formula, and observed by a microplate reader. The viability of the cells was observed by an inverted TMS microscope with 100x magnification. The MTT assay’s test shows that the HUCMSCs cell viability shed each variant of HA-TCP scaffold dose, did not show any ...

Research Journal of Pharmacy and Technology

Polymethyl Methacrylate (PMMA) and Hydroxyapatite (HA) utilization as single materials are rarely... more Polymethyl Methacrylate (PMMA) and Hydroxyapatite (HA) utilization as single materials are rarely used as dental implant materials. There is a promising hope by combining these two materials as a dental implant fixture. Nevertheless, there is a limited information of PMMA/HA composite utilization as dental implant material. The aims of this narrative review is to describe the potential of PMMA/HA composite utilization as biomaterial candidate for porous trabecular dental implant fixture development. This narrative review finds the potential of PMMA/HA composite as biomaterial candidate for porous trabecular dental implant. The keywords "Biomaterial," "Dental Implant," "Hydroxyapatite," "Osseointegration," and "Polymethyl Methacrylate" were used in a web-based search of PubMed, NCBI, Scopus, ScienceDirect, and ResearchGate databases. PMMA is non-toxic, cost-effective, biocompatible, simple to manipulate, and has strong mechanical prop...

Journal of oral biology and craniofacial research, Mar 1, 2023

Excessive gingival pigmentation is a major aesthetic concern in modern society, though it is not ... more Excessive gingival pigmentation is a major aesthetic concern in modern society, though it is not a medical problem they consider it as a negative attribute. Patients with gingival hyperpigmentation usually complain and request cosmetic therapy, particularly if the pigmentation is visible during speaking and smiling. Various depigmentation methods, including burr abrasion, cryosurgery, electro-surgery, split thickness flap excision and surgical scraping techniques have been used with varying degrees of success. Recently, lasers have been used to ablate cells containing and producing the melanin pigment. The present study was undertaken to compare the clinical effectiveness and patient comfort of surgical scrapping and diode laser technique used for gingival depigmentation for a follow up period of 6 months.20 subjects participated in this split mouth study. The clinical evaluation parameters included Extent and Intensity of gingival hyperpigmentation, post-operative gingival bleeding and pain. On follow up examination at 6 th month there was no statistical difference in repigmentation extent and intensity between diode laser and surgical scraping techniques. The mean pain scores for treated sites with diode laser were significantly lower than surgical scrapping technique at 24 hours (t-value=2.430, p-value=0.02). The postoperative gingival bleeding at end of procedure was significantly lower with diode laser than surgical scrapping technique (p-value=<0.0001). There was no statistical difference in postoperative re-pigmentation and clinical efficacy among the subjects between surgical scraping and diode laser technique at 6 th month follow up. Diode laser technique provides better haemostasis and good visibility at the surgical site. The post-operative patient comfort is better at the surgical sites treated with diode laser than surgical scrapping method. Hence, both the techniques are used for depigmentation procedures depending on the severity and gingival biotype and patient acceptance.

European Journal of Dentistry, Jun 19, 2023

Objectives Stem cell from human exfoliated deciduous teeth (SHED) has great potential for bone ti... more Objectives Stem cell from human exfoliated deciduous teeth (SHED) has great potential for bone tissue engineering and cell therapy for regenerative medicine. It has been combined with biomaterials such as mixed of polymethylmethacrylate (PMMA) and hydroxyapatite (HA) as candidates for synthetic bone graft biomaterial. The aim of this study was to analyze the toxicity test of mixed PMMA-HA scaffold seeded with SHED and osteoblast in vitro. Materials and Methods SHED was isolated from the pulp of noncarious deciduous teeth and osteoblast cells were cultured, and exposed to PMMA-HA scaffolds with three concentration groups: 20/80, 30/70, and 40/60 for 24 hours. Cytotoxicity test was performed by MTT assay to cell viability. Statistical Analysis Data were analyzed using IBM SPSS Statistics 25, one-way analysis of variance followed by least significant difference test, considering the level of significance p-value less than 0.05 Results The percentage of SHED's viability was best in the PMMA-HA group with concentrations of 20/80, followed by 30/70, and 40/60 with 87.03, 75.33, and 65.79%, respectively. The percentage of osteoblast cell's viability was best in the PMMA-HA group with concentrations of 20/80, followed by 30/70, and 40/60 with 123.6, 108.36, and 93.48%, respectively. Conclusions Mixed PMMA-HA was not toxic for the SHED and osteoblast. This characteristic is the initial requirement to be proposed as an alternative material for healing alveolar bone defects. In vivo animal research is mandatory to confirm the use of PMMA-HA on the alveolar defect model.

Journal of oral biology and craniofacial research, Mar 1, 2023

Nucleation and Atmospheric Aerosols, 2020

Research Journal of Pharmacy and Technology, Jan 27, 2023

Background: Bone graft materials are widely used to support the success of periodontal tissue tre... more Background: Bone graft materials are widely used to support the success of periodontal tissue treatments. Graft material has been used extensively however it has some deficiencies such as limited availability, high levels of resorption, immunological reactions and the risk of contamination as well as high costs. Therefore recent research is needed to explore potential usage of the tooth graft. Objective(s): The objective of this research is to comparing the MMP-13 expression in bone healing after grafting the incisor’s extraction sockets with hydroxyapatite tooth graft and hydroxyapatite xenograft. Research Methods: 33 adult male Cavia cobayas were divided into three groups: hydroxyapatite xenograft, hydroxyapatite tooth graft, and control (without treatment). Bone tissue sections were stained with diaminobenzidine and then dripping with anti-MMP-13 monoclonal antibody. The number of MMP-13 expression by osteoblast cells was carried out quantitatively based on the colourintensity in each group under the OLYMPUS microscope with 400x magnification. Results: The value of MMP-13 expression was higher on the tooth graft and xenograft group than the control group. The ability of osteoconduction of hydroxyapatite derived from porous hydroxyapatite structure will facilitate angiogenesis so that osteogenic cells can migrate and attach to the graft to induce the new bone growth. The tooth graft group had higher MMP-13 value than xenograft group. High solubility of graft material could affect bone remodeling and mineralization processes because of the presence of inflammatory cells is detrimental to the healing process. Conclusion: As a new graft material, tooth graft has a capability to induce osteoconductive and osteoinductive that similar with the xenograft.

Background: Chitosan is a natural polymer with favourable properties such as biocompatibility, bi... more Background: Chitosan is a natural polymer with favourable properties such as biocompatibility, biodegradability, non- allergenic, and non- toxic which make it a very promising material for scaffold in tissue engineering. Application of chitosan based systems in hard tissue engineering such as bone, cartilage, and periodontal are reviewd with recent and relevant examples. Hydroxiapatite is a polymer of calcium phosphates from bones and can be extracted cortical bovine bone. Hydroxiapatite has poor solubility, so it is combined with chitosan because chitosan has high solubility to make good strength scaffold for bone healing.. Purpose: The purpose of this laboratory research is to study the density of collagen on bone with the application of hydroxyapatit chitosan and hydroxyapatite. Methods:. The samples used were 24 healthy 3 months old male wistar’ s rats, with approximately 200 grams of body weight and they were devided into 4 groups. Two groups were taken for hydroxiapatite chitosan and the others for hydroxiapatite. A group were taken for hydroxiapatite chitosan was given for 7 days in group C7, C14 for 14 days. A group were taken for hydroxyapatite was given for 7 days in group H7, H14 for 14 days. Seven days of operation time was waiting before the sample being decapited for further histological examination in the post operation. A hematoxillin staining was used and the density of collagen counted under light microscopy with 400 times magnification T- test and least significant difference test were used for the statistic evaluation. Result:. shown increase of the density of collagen in hydroxiapatite chitosan group of 7 days and the hydroxiapatite chitosan of 14 days is decrease. On the other hand, the result of study shown decrease of the density of collagen in hydroxiapatite group of 14 days and it increase for the group of 7 days. This study that duration time of hydroxiapatite and chitosan had not been interfered significantly with the density of collagen during bone healing after the defect in wistar’s rats. Conclusion: There is no different between hydroxyapatite and hydroxyapatite chitosan application to the density of collagen. Chitosan only can accelerate the bone healing in proliferation phase. Key words: Hydroxyapatite, chitosan, collagen.

THE 2ND INTERNATIONAL CONFERENCE ON PHYSICAL INSTRUMENTATION AND ADVANCED MATERIALS 2019

THE 2ND INTERNATIONAL CONFERENCE ON PHYSICAL INSTRUMENTATION AND ADVANCED MATERIALS 2019, 2020

In the case of tooth loss ideally it is replaced with a dental implant, because dental impants ha... more In the case of tooth loss ideally it is replaced with a dental implant, because dental impants have several advantages such as restoring the function of mastication, increasing mobility and reducing pain. Nowadays after tooth extraction, dental implants are often implanted immediately, with the consideration of preventing mechanical loading, thus obtaining the stability needed for osseointergation of the implant. Titanium (Ti) implants are the most commonly used materials because they have good mechanical properties, biocompability, stable chemical, low toxicity, bioactivity and corrosion resistance. Various implant surface modification materials were carried out to improve implant integration, including hydroxyapatite (HA), antibacterial and BMP-2. These coatings to improve osseointegration and avoid implant infection, thus increasing the success rate of the implant.

Background. Periodontal disease happens approximately at 10% of adult populations, characterized ... more Background. Periodontal disease happens approximately at 10% of adult populations, characterized by loss of attachment beetwen tooth and bone, resulting in bone resorbtion and eventually bone loss. In the wound healing process to repair bone tissue, macrophage have an important role. To facilitate the healing process, bone graft is placed in the bone defect. Compounding hydroxyapatite-chitosan can result in better osteoconductivity properties and scaffold strength than both of material stands alone. Purpose. The aim of this study was to know the effect of hydroxyapatite-chitosan aplication on macrophage proliferation in bone remodelling. Method. This research was done in vivo experiment by created a defect on rabbit’s tibiae. 20 rabbits were divide into 4 groups The 1st group treated with 70:30 hydroxyapatite -chitosan powder, examined after 4 days. 2nd treated with 30:70 hydroxyapatite -chitosan powder, examined after 4 days, 3th group treated with 70:30 hydroxyapatite-chitosan pow...

Nigerian Journal of Clinical Practice, 2021

Background: Periodontal regeneration is the anticipated result of periodontal tissue therapy. Of ... more Background: Periodontal regeneration is the anticipated result of periodontal tissue therapy. Of the many scaffold materials utilized, collagen has been shown to possess characteristics that strongly promote the periodontal regeneration process. Gourami (Osphronemusgoramy) scales can be utilized as an alternative source of collagen due to their abundant availability, the absence of risk of zoonosis, and lack of religious strictures against their use. Aims: To analyze the characteristics of collagen extracted from gourami scales. Methods and Materials: A combination of scanning electron microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR), and in vitro enzymatic biodegradation analysis were employed to determine pore sizes, constituent functional groups, and the degradation rate of collagen extractedfrom gourami scales. Results: Collagen extracted from gourami scales demonstrated an average porosity of 13.779 μm. The FTIR test results indicated Amide A, Amide B, Amide I, ...

Dental Journal (Majalah Kedokteran Gigi)

Background: Periodontitis is a chronic inflammatory disease of the periodontal tissue that is cha... more Background: Periodontitis is a chronic inflammatory disease of the periodontal tissue that is characterized by alveolar bone resorption. This occurs due to an imbalance of osteoblast and osteoclast during the bone formation and resorption processes. In order to obtain complete regeneration of periodontal tissue, bone grafting is frequently used in periodontal surgical therapy. Although each material has disadvantages, safe graft materials derived from animal sources can be employed as an alternative to bone graft materials. Osteoblast, osteoclast, calcified bone matrix, type I collagen, osteonectin, and hydroxyapatite can all be found in gourami scales, a form of food waste. BMP4 has osteoinduction functions, which are important in bone metabolism. Through angiogenic activity, FGF2 also contributes to periodontal regeneration. Purpose: The aim of the study was to assess the expression of BMP4 and FGF2 after the treatment group had been given gourami fish scale extract. Methods: Thir...

Global Journal of Health Science, 2016

Excessive gingival pigmentation is a major aesthetic concern in modern society, though it is not ... more Excessive gingival pigmentation is a major aesthetic concern in modern society, though it is not a medical problem they consider it as a negative attribute. Patients with gingival hyperpigmentation usually complain and request cosmetic therapy, particularly if the pigmentation is visible during speaking and smiling. Various depigmentation methods, including burr abrasion, cryosurgery, electro-surgery, split thickness flap excision and surgical scraping techniques have been used with varying degrees of success. Recently, lasers have been used to ablate cells containing and producing the melanin pigment. The present study was undertaken to compare the clinical effectiveness and patient comfort of surgical scrapping and diode laser technique used for gingival depigmentation for a follow up period of 6 months.20 subjects participated in this split mouth study. The clinical evaluation parameters included Extent and Intensity of gingival hyperpigmentation, post-operative gingival bleeding...

Journal of Pharmacy & Pharmacognosy Research

Context: The most common biomaterial used for dental implants is titanium. However, the release o... more Context: The most common biomaterial used for dental implants is titanium. However, the release of metal ions and the risk of allergic reactions to metals that may occur in some patients cannot be avoided. Hydroxyapatite-polymethylmethacrylate (HA-PMMA) composite biomaterials are proposed to have potential as dental implant biomaterials due to their mechanical, chemical, and biological properties. HA-PMMA may induce osseointegration, biocompatible, less allergic reactions, and no metal ions released. In addition, HA-PMMA can be obtained from Indonesia’s abundant natural resources. Aims: To explore HA-PMMA composites through molecular docking as a biomaterial candidate for dental implants in silico. Methods: Structure data format (sdf), molecular weight, and identity number (CID) of HA-PMMA ligand samples were obtained from PubChem database and minimized through OpenBabel. 3D structure, selection method, resolution, atom count, weight, sequence length, and ID protein BMP2, BMP4, BMP7...

Research journal of pharmacy and technology, Aug 30, 2022

Research Journal of Pharmacy and Technology

Background and Aim: The current goal of periodontal therapy is to achieve periodontal regeneratio... more Background and Aim: The current goal of periodontal therapy is to achieve periodontal regeneration. Important factor for periodontal regeneration is to promote bone formation, nowadays used bone replacement. The used of xenograft materials as gold standard for periodontal tissue regeneration using type I collagen bone graft has been widely developed. The main organic component in fish scales is type I fibril collagen, which are same as with component in bones. Specific markers of bone formation are the expression of osteoblast, osteoclast, osteoprotegerin (OPG), and receptor activator of nuclear factor κB ligands (RANKL). The purpose of this study is to assess the expression of OPG and RANKL after application of extract collagen from gourami (Osphronemus gouramy) scales. Materials and Methods: Thirty-two experimental 3-month-old male Wistar albino rats (150g and 200g) were randomly divided into four groups: 7-day control group, 7-day fish collagen group, 14-day control group and 14-...

International Journal of Integrated Engineering

Hydroxyapatite-tricalcium phosphate (HA-TCP) scaffold is a three-dimensional structure used to su... more Hydroxyapatite-tricalcium phosphate (HA-TCP) scaffold is a three-dimensional structure used to support bone regeneration. Ideally, the scaffold should be biocompatible, biodegradable, and non-toxic. The tissue engineering technique uses a combined stem cell and scaffold to repair the bone defect. To prove the non-toxic properties of the scaffold, a cytotoxicity test is needed for the human umbilical cord mesenchymal stem cell (HUCMSCs). In this study, 27 samples were divided into eight groups with a variant of scaffold HA-TCP doses ranging from 5–1000 µg. Each treatment group of scaffolds was covered in HUCMSCs. The samples were added by methyl-thiazol-tetrazolium (MTT) colorimetric assay, counted using an optical density (OD) formula, and observed by a microplate reader. The viability of the cells was observed by an inverted TMS microscope with 100x magnification. The MTT assay’s test shows that the HUCMSCs cell viability shed each variant of HA-TCP scaffold dose, did not show any ...

Research Journal of Pharmacy and Technology

Polymethyl Methacrylate (PMMA) and Hydroxyapatite (HA) utilization as single materials are rarely... more Polymethyl Methacrylate (PMMA) and Hydroxyapatite (HA) utilization as single materials are rarely used as dental implant materials. There is a promising hope by combining these two materials as a dental implant fixture. Nevertheless, there is a limited information of PMMA/HA composite utilization as dental implant material. The aims of this narrative review is to describe the potential of PMMA/HA composite utilization as biomaterial candidate for porous trabecular dental implant fixture development. This narrative review finds the potential of PMMA/HA composite as biomaterial candidate for porous trabecular dental implant. The keywords "Biomaterial," "Dental Implant," "Hydroxyapatite," "Osseointegration," and "Polymethyl Methacrylate" were used in a web-based search of PubMed, NCBI, Scopus, ScienceDirect, and ResearchGate databases. PMMA is non-toxic, cost-effective, biocompatible, simple to manipulate, and has strong mechanical prop...