Ahmed I. Mehrim | Mansoura University (original) (raw)
Papers by Ahmed I. Mehrim
Frontiers in Marine Science
The hybrid red tilapia (♂Oreochromis niloticus × ♀O. mossambicus) is a promising tilapia fish wit... more The hybrid red tilapia (♂Oreochromis niloticus × ♀O. mossambicus) is a promising tilapia fish with high growth rate, market acceptability, and the ability to live in a wide range of salinities, but it is sensitive to low water temperature. Therefore, the objective of this experiment was to improve hybrid red tilapia resistance to chronic cold stress by increasing unsaturated fatty acids and evaluating their effects on fish body weight, hepatosomatic index (HSI), body nutrient composition, liver function enzymes, histopathological alterations, and biomarkers of oxidative stress in the gills and liver. For 98 days, fish were fed four diets based on two dietary fat sources: corn oil (a traditional source) and Aquafat-O® (a commercial product rich in high polyunsaturated fatty acids), in different ratios of 3:0 (T0), 2:1 (T1), 1:2 (T2), and 0:3 (T3), respectively. Subsequently, all groups (T0+, T1, T2, and T3) were subjected to water cold stress at 13 °C for 15 days, with half of the co...
Aquaculture Nutrition
Azolla is a potential fish feed ingredient due to its high nutritional value, abundant production... more Azolla is a potential fish feed ingredient due to its high nutritional value, abundant production, and low price. This study is aimed at evaluating the use of fresh green azolla (FGA) as a replacement ratio of the daily feed intake on the growth, digestive enzymes, hematobiochemical indices, antioxidant response, intestinal histology, body composition, and flesh quality of monosex Nile tilapia, Oreochromis niloticus (with an average initial weight of 108.0 ± 5.0 g ). Five experimental groups were used and differed in commercial feed replacement rates of 0% ( T 0 ), 10% ( T 1 ), 20% ( T 2 ), 30% ( T 3 ), and 40% ( T 4 ) with FGA for 70 days. Results showed that 20% replacement with azolla gave the highest values of growth performance and hematological parameters and the best feed conversion ratio, protein efficiency ratio, and fish whole body protein content. The highest levels of intestinal chymotrypsin, trypsin, lipase, and amylase were noted in 20% replacement with azolla. Fish ...
Sustainability
Aquaculture is an important component of the human diet, providing high-quality aquatic food for ... more Aquaculture is an important component of the human diet, providing high-quality aquatic food for global or local consumption. Egypt is one of the countries most vulnerable to the potential impacts of climate change (CC), especially in the aquaculture sector. CC is one of the biggest challenges of our time and has negatively affected different water bodies. CC leads to the combination of changes in water availability, a decrease in water quality, the movement of salt water upstream due to rising sea levels, and the salinization of groundwater supplies will threaten inland freshwater aquaculture. Similarly, higher temperatures resulting from CC lead to reduce dissolved oxygen levels, increased fish metabolic rates, increased risk of disease spread, increased fish mortality, and consequently decreased fish production. CC may also indirectly affect aquaculture activities; for example, large areas of lowland aquaculture ponds can be highly vulnerable to flooding from rising sea levels. T...
International Journal of Current Research in Biosciences and Plant Biology, 2016
Asian Journal of Animal and Veterinary Advances, 2014
Journal of Fisheries and Aquatic Science, 2016
Aflatoxins are known as a hepatocarcinogen in various animal species, including fish. The present... more Aflatoxins are known as a hepatocarcinogen in various animal species, including fish. The present study was designed to evaluate the ability of some medicinal herbs against aflatoxicosis regarding clinical signs, postmortem examination and microscopic findings in liver of all male mono-sex Oreochromis niloticus. A total of 360 fish (10.00 ± 0.38g) were randomly distributed into eight groups (three replicates for each), stocked at 15 fish per aquarium, fish group (G1) being the control and fed a basal ration (BR) free from aflatoxin B1 (AFB1) and G2 fed aflatoxicated BR (150 ppb AFB1). Other fish groups fed aflatoxicated BR (150 ppb AFB1) supplemented with 1% of each tested medicine herbs, black seed (Nigella sativa) (G3); liquorice (Glycyrrhiza glabra) (G4); onion (Allium cepa) (G5); garlic (Allium sativum) (G6); fenugreek (Trigonella foenum-graecum) (G7) and ceylon cinnamon (Cinnamomum verum) (G8). Results revealed severe toxic effects of AFB1 on fish besides the hepatotoxic effect...
Fish Physiology and Biochemistry
Egyptian Journal of Aquatic Biology and Fisheries
Aflatoxins are known as a hepatocarcinogen in various animal species, including fish. The present... more Aflatoxins are known as a hepatocarcinogen in various animal species, including fish. The present study was designed to evaluate the ability of some medicinal herbs against aflatoxicosis regarding clinical signs, postmortem examination and microscopic findings in liver of all male mono-sex Oreochromis niloticus. A total of 360 fish (10.00 ± 0.38g) were randomly distributed into eight groups (three replicates for each), stocked at 15 fish per aquarium, fish group (G 1) being the control and fed a basal ration (BR) free from aflatoxin B 1 (AFB 1) and G 2 fed aflatoxicated BR (150 ppb AFB 1). Other fish groups fed aflatoxicated BR (150 ppb AFB 1) supplemented with 1% of each tested medicine herbs, black seed (Nigella sativa) (G 3); liquorice (Glycyrrhiza glabra) (G 4); onion (Allium cepa) (G 5); garlic (Allium sativum) (G 6); fenugreek (Trigonella foenum-graecum) (G 7) and ceylon cinnamon (Cinnamomum verum) (G 8). Results revealed severe toxic effects of AFB 1 on fish besides the hepatotoxic effects on the liver compared with the control group. Meanwhile, dietary medicinal herbs led to eliminate the toxic effects of AFB 1 on fish, especially black seed or ceylon cinnamon. So, it could conclude the safety and useful dietary addition of 1% black seed (Nigella sativa, G 3) or ceylon cinnamon (Cinnamomum verum, G 8) to alleviate the toxic effects of AFB 1 on O. niloticus fingerlings.
Th is study was conducted to evaluate the effects of some unconventional feedstuffs on blood pict... more Th is study was conducted to evaluate the effects of some unconventional feedstuffs on blood picture, whole fish and fish muscles composition and total counts of pathogenic microorganisms of Nile tilapia fingerlings. Fish with 7-8g as an initial body weight were stocked at a rate of 7 fingerlings per aquarium (60×35×40cm). The basal diet contained 25% crude protein. The diets were offered daily at two meals at 3% of fish body weight. Each dietary treatment was performed in two aquaria for sixteen weeks. The experimental diets were nearly isocaloric and isonitrogenus. The 1 st diet was a control, diets No. 2-5 were the control diet but their fishmeal was substituted by 25, 50, 75 and 100%, respectively with duckweed meal (DW), diets No. 6-9 included crayfish meal (C r F i) at the same previous replacement rates, and diets No. 10-13 included a mixture of DW + C r F i (1:1) as a substitute for fishmeal at the same rates. The important results refereed to diet No.6 as by reflected highest values of hemoglobin, hematocrit, total protein, and globulin, but diet No.4 gave the lowest values. Transaminases activity were increased with diet No.5 and decreased with diet No.7 reflecting to affect hepatic function. The 5 th diet also increased blood levels of urea, uric acid and creatinine, reflecting to affect kidney function. Carcass and muscle composition of the tested fish significantly affected by dietary treatments. The highest dry matter was determined in the 5 th group but the lowest in the 6 th group. Diets No.6 and 4 were responsible for the highest and the lowest protein %, respectively. Diets No.5 and 6 reflected the highest and lowest ether extract %, respectively. The lowest ash % was estimated for the 4 th and 5 th groups, whereas the highest with diet No.7. There were positive relationships between moisture and protein as well as between moisture and ash contents, but negative relationships were found between moisture and ether extract as well as between protein and ether extract % in whole fish body and fish muscles. Decreased silica level of the 2 nd diet led to low silica content of fish body of this group. The highest silica level was found in the 9 th diet; yet, the highest silica content was recorded in the 13 th fish group. The highest lead content was found in diet of the 9 th group, but in fish of the 7 th and 13 th groups. The lowest lead content in diet and fish was found of the 2 nd group. The 13 th group reflected the highest cadmium levels in diet and fish, but the lowest levels in diet and fish were found of the control group. The microbiological test revealed no critical counts of pathogenic organisms, whether in water, diet or fish tissues.
... fish was measured biweekly to point feed quantity and to calculate growth performance accordi... more ... fish was measured biweekly to point feed quantity and to calculate growth performance according to Abdelhamid (2000) in form of: average weight gain (g/fish) AWG = average final weight (g) average initial weight (g), average daily gain, (g/fish/day) ADG = AWG ...
Journal of Biological Sciences
The objective of the present study was to determine the effects of dietary inclusion of different... more The objective of the present study was to determine the effects of dietary inclusion of different levels of pawpaw seeds powder (PSP) at different periods on the growth performance, survival rate, feed utilization and carcass composition of fry and fingerlings of Nile tilapia (Oreochromis niloticus). The experiment was divided into two periods; the first was feeding period of Nile tilapia fry with PSP for 71 days; and, the second was rearing period where the Nile tilapia fingerlings fed control diet (0 g/kg diet from PSP) for 84 days. Apparently-healthy 1200 Nile tilapia fry of one day old with an average initial body weight 0.012 g/fry were randomly distributed to 20 treatments. Each treatment was referred to three aquaria. The PSP was added to the basal diet at levels of 0, 2, 4, 6 and 8 g/kg diet for 15, 30, 45 and 60 days, different periods.
This study was conducted to investigate the toxic effects of aflatoxin B 1 (AFB 1 ) on mono-sex N... more This study was conducted to investigate the toxic effects of aflatoxin B 1 (AFB 1 ) on mono-sex Nile tilapia Oreochromis niloticus fingerlings and attempting to detoxify these drastic effects by using some nutritious agents. Therefore, half percent of each of these nutritious agents namely Bio-Buds-2x, chamomile flowers, aspirin and ginger were added to aflatoxic (100 ppb aflatoxin B 1 ) diet for fingerlings. These diets were offered 6 days a week at 3% daily of actual biomass in glass aquaria in duplicate/treatment in an indoor feeding experiment lasted 14 weeks. The aflatoxic diet led to the worst fish growth performance and survival rate, feed and protein utilization, internal organs indices, carcass composition and residues of AFB 1 (ppb) in the whole body of fish and the tested parameters of blood haematology and biochemistry of the experimented fish. Dietary ginger inclusion alleviated aflatoxicosis symptoms by fish, since it improved all the above tested parameters of aflatoixcated fish. Generally, the obtained results in the present study indicated that the ginger was the best detoxifying agent of aflatoxin, followed by aspirin and chamomile flowers respectively.
This study was conducted to investigate the toxic effects of aflatoxin B 1 (AFB 1 ) on mono-sex N... more This study was conducted to investigate the toxic effects of aflatoxin B 1 (AFB 1 ) on mono-sex Nile tilapia Oreochromis niloticus fingerlings and attempting to detoxify these drastic effects by using some nutritious agents. Therefore, half percent of each of these nutritious agents namely Bio-Buds-2x, chamomile flowers, aspirin and ginger were added to aflatoxic (100 ppb aflatoxin B 1 ) diet for fingerlings. These diets were offered 6 days a week at 3% daily of actual biomass in glass aquaria in duplicate/treatment in an indoor feeding experiment lasted 14 weeks. The aflatoxic diet led to the worst fish growth performance and survival rate, feed and protein utilization, internal organs indices, carcass composition and residues of AFB 1 (ppb) in the whole body of fish and the tested parameters of blood haematology and biochemistry of the experimented fish. Dietary ginger inclusion alleviated aflatoxicosis symptoms by fish, since it improved all the above tested parameters of aflatoixcated fish. Generally, the obtained results in the present study indicated that the ginger was the best detoxifying agent of aflatoxin, followed by aspirin and chamomile flowers respectively.
... fish was measured biweekly to point feed quantity and to calculate growth performance accordi... more ... fish was measured biweekly to point feed quantity and to calculate growth performance according to Abdelhamid (2000) in form of: average weight gain (g/fish) AWG = average final weight (g) average initial weight (g), average daily gain, (g/fish/day) ADG = AWG ...
Frontiers in Marine Science
The hybrid red tilapia (♂Oreochromis niloticus × ♀O. mossambicus) is a promising tilapia fish wit... more The hybrid red tilapia (♂Oreochromis niloticus × ♀O. mossambicus) is a promising tilapia fish with high growth rate, market acceptability, and the ability to live in a wide range of salinities, but it is sensitive to low water temperature. Therefore, the objective of this experiment was to improve hybrid red tilapia resistance to chronic cold stress by increasing unsaturated fatty acids and evaluating their effects on fish body weight, hepatosomatic index (HSI), body nutrient composition, liver function enzymes, histopathological alterations, and biomarkers of oxidative stress in the gills and liver. For 98 days, fish were fed four diets based on two dietary fat sources: corn oil (a traditional source) and Aquafat-O® (a commercial product rich in high polyunsaturated fatty acids), in different ratios of 3:0 (T0), 2:1 (T1), 1:2 (T2), and 0:3 (T3), respectively. Subsequently, all groups (T0+, T1, T2, and T3) were subjected to water cold stress at 13 °C for 15 days, with half of the co...
Aquaculture Nutrition
Azolla is a potential fish feed ingredient due to its high nutritional value, abundant production... more Azolla is a potential fish feed ingredient due to its high nutritional value, abundant production, and low price. This study is aimed at evaluating the use of fresh green azolla (FGA) as a replacement ratio of the daily feed intake on the growth, digestive enzymes, hematobiochemical indices, antioxidant response, intestinal histology, body composition, and flesh quality of monosex Nile tilapia, Oreochromis niloticus (with an average initial weight of 108.0 ± 5.0 g ). Five experimental groups were used and differed in commercial feed replacement rates of 0% ( T 0 ), 10% ( T 1 ), 20% ( T 2 ), 30% ( T 3 ), and 40% ( T 4 ) with FGA for 70 days. Results showed that 20% replacement with azolla gave the highest values of growth performance and hematological parameters and the best feed conversion ratio, protein efficiency ratio, and fish whole body protein content. The highest levels of intestinal chymotrypsin, trypsin, lipase, and amylase were noted in 20% replacement with azolla. Fish ...
Sustainability
Aquaculture is an important component of the human diet, providing high-quality aquatic food for ... more Aquaculture is an important component of the human diet, providing high-quality aquatic food for global or local consumption. Egypt is one of the countries most vulnerable to the potential impacts of climate change (CC), especially in the aquaculture sector. CC is one of the biggest challenges of our time and has negatively affected different water bodies. CC leads to the combination of changes in water availability, a decrease in water quality, the movement of salt water upstream due to rising sea levels, and the salinization of groundwater supplies will threaten inland freshwater aquaculture. Similarly, higher temperatures resulting from CC lead to reduce dissolved oxygen levels, increased fish metabolic rates, increased risk of disease spread, increased fish mortality, and consequently decreased fish production. CC may also indirectly affect aquaculture activities; for example, large areas of lowland aquaculture ponds can be highly vulnerable to flooding from rising sea levels. T...
International Journal of Current Research in Biosciences and Plant Biology, 2016
Asian Journal of Animal and Veterinary Advances, 2014
Journal of Fisheries and Aquatic Science, 2016
Aflatoxins are known as a hepatocarcinogen in various animal species, including fish. The present... more Aflatoxins are known as a hepatocarcinogen in various animal species, including fish. The present study was designed to evaluate the ability of some medicinal herbs against aflatoxicosis regarding clinical signs, postmortem examination and microscopic findings in liver of all male mono-sex Oreochromis niloticus. A total of 360 fish (10.00 ± 0.38g) were randomly distributed into eight groups (three replicates for each), stocked at 15 fish per aquarium, fish group (G1) being the control and fed a basal ration (BR) free from aflatoxin B1 (AFB1) and G2 fed aflatoxicated BR (150 ppb AFB1). Other fish groups fed aflatoxicated BR (150 ppb AFB1) supplemented with 1% of each tested medicine herbs, black seed (Nigella sativa) (G3); liquorice (Glycyrrhiza glabra) (G4); onion (Allium cepa) (G5); garlic (Allium sativum) (G6); fenugreek (Trigonella foenum-graecum) (G7) and ceylon cinnamon (Cinnamomum verum) (G8). Results revealed severe toxic effects of AFB1 on fish besides the hepatotoxic effect...
Fish Physiology and Biochemistry
Egyptian Journal of Aquatic Biology and Fisheries
Aflatoxins are known as a hepatocarcinogen in various animal species, including fish. The present... more Aflatoxins are known as a hepatocarcinogen in various animal species, including fish. The present study was designed to evaluate the ability of some medicinal herbs against aflatoxicosis regarding clinical signs, postmortem examination and microscopic findings in liver of all male mono-sex Oreochromis niloticus. A total of 360 fish (10.00 ± 0.38g) were randomly distributed into eight groups (three replicates for each), stocked at 15 fish per aquarium, fish group (G 1) being the control and fed a basal ration (BR) free from aflatoxin B 1 (AFB 1) and G 2 fed aflatoxicated BR (150 ppb AFB 1). Other fish groups fed aflatoxicated BR (150 ppb AFB 1) supplemented with 1% of each tested medicine herbs, black seed (Nigella sativa) (G 3); liquorice (Glycyrrhiza glabra) (G 4); onion (Allium cepa) (G 5); garlic (Allium sativum) (G 6); fenugreek (Trigonella foenum-graecum) (G 7) and ceylon cinnamon (Cinnamomum verum) (G 8). Results revealed severe toxic effects of AFB 1 on fish besides the hepatotoxic effects on the liver compared with the control group. Meanwhile, dietary medicinal herbs led to eliminate the toxic effects of AFB 1 on fish, especially black seed or ceylon cinnamon. So, it could conclude the safety and useful dietary addition of 1% black seed (Nigella sativa, G 3) or ceylon cinnamon (Cinnamomum verum, G 8) to alleviate the toxic effects of AFB 1 on O. niloticus fingerlings.
Th is study was conducted to evaluate the effects of some unconventional feedstuffs on blood pict... more Th is study was conducted to evaluate the effects of some unconventional feedstuffs on blood picture, whole fish and fish muscles composition and total counts of pathogenic microorganisms of Nile tilapia fingerlings. Fish with 7-8g as an initial body weight were stocked at a rate of 7 fingerlings per aquarium (60×35×40cm). The basal diet contained 25% crude protein. The diets were offered daily at two meals at 3% of fish body weight. Each dietary treatment was performed in two aquaria for sixteen weeks. The experimental diets were nearly isocaloric and isonitrogenus. The 1 st diet was a control, diets No. 2-5 were the control diet but their fishmeal was substituted by 25, 50, 75 and 100%, respectively with duckweed meal (DW), diets No. 6-9 included crayfish meal (C r F i) at the same previous replacement rates, and diets No. 10-13 included a mixture of DW + C r F i (1:1) as a substitute for fishmeal at the same rates. The important results refereed to diet No.6 as by reflected highest values of hemoglobin, hematocrit, total protein, and globulin, but diet No.4 gave the lowest values. Transaminases activity were increased with diet No.5 and decreased with diet No.7 reflecting to affect hepatic function. The 5 th diet also increased blood levels of urea, uric acid and creatinine, reflecting to affect kidney function. Carcass and muscle composition of the tested fish significantly affected by dietary treatments. The highest dry matter was determined in the 5 th group but the lowest in the 6 th group. Diets No.6 and 4 were responsible for the highest and the lowest protein %, respectively. Diets No.5 and 6 reflected the highest and lowest ether extract %, respectively. The lowest ash % was estimated for the 4 th and 5 th groups, whereas the highest with diet No.7. There were positive relationships between moisture and protein as well as between moisture and ash contents, but negative relationships were found between moisture and ether extract as well as between protein and ether extract % in whole fish body and fish muscles. Decreased silica level of the 2 nd diet led to low silica content of fish body of this group. The highest silica level was found in the 9 th diet; yet, the highest silica content was recorded in the 13 th fish group. The highest lead content was found in diet of the 9 th group, but in fish of the 7 th and 13 th groups. The lowest lead content in diet and fish was found of the 2 nd group. The 13 th group reflected the highest cadmium levels in diet and fish, but the lowest levels in diet and fish were found of the control group. The microbiological test revealed no critical counts of pathogenic organisms, whether in water, diet or fish tissues.
... fish was measured biweekly to point feed quantity and to calculate growth performance accordi... more ... fish was measured biweekly to point feed quantity and to calculate growth performance according to Abdelhamid (2000) in form of: average weight gain (g/fish) AWG = average final weight (g) average initial weight (g), average daily gain, (g/fish/day) ADG = AWG ...
Journal of Biological Sciences
The objective of the present study was to determine the effects of dietary inclusion of different... more The objective of the present study was to determine the effects of dietary inclusion of different levels of pawpaw seeds powder (PSP) at different periods on the growth performance, survival rate, feed utilization and carcass composition of fry and fingerlings of Nile tilapia (Oreochromis niloticus). The experiment was divided into two periods; the first was feeding period of Nile tilapia fry with PSP for 71 days; and, the second was rearing period where the Nile tilapia fingerlings fed control diet (0 g/kg diet from PSP) for 84 days. Apparently-healthy 1200 Nile tilapia fry of one day old with an average initial body weight 0.012 g/fry were randomly distributed to 20 treatments. Each treatment was referred to three aquaria. The PSP was added to the basal diet at levels of 0, 2, 4, 6 and 8 g/kg diet for 15, 30, 45 and 60 days, different periods.
This study was conducted to investigate the toxic effects of aflatoxin B 1 (AFB 1 ) on mono-sex N... more This study was conducted to investigate the toxic effects of aflatoxin B 1 (AFB 1 ) on mono-sex Nile tilapia Oreochromis niloticus fingerlings and attempting to detoxify these drastic effects by using some nutritious agents. Therefore, half percent of each of these nutritious agents namely Bio-Buds-2x, chamomile flowers, aspirin and ginger were added to aflatoxic (100 ppb aflatoxin B 1 ) diet for fingerlings. These diets were offered 6 days a week at 3% daily of actual biomass in glass aquaria in duplicate/treatment in an indoor feeding experiment lasted 14 weeks. The aflatoxic diet led to the worst fish growth performance and survival rate, feed and protein utilization, internal organs indices, carcass composition and residues of AFB 1 (ppb) in the whole body of fish and the tested parameters of blood haematology and biochemistry of the experimented fish. Dietary ginger inclusion alleviated aflatoxicosis symptoms by fish, since it improved all the above tested parameters of aflatoixcated fish. Generally, the obtained results in the present study indicated that the ginger was the best detoxifying agent of aflatoxin, followed by aspirin and chamomile flowers respectively.
This study was conducted to investigate the toxic effects of aflatoxin B 1 (AFB 1 ) on mono-sex N... more This study was conducted to investigate the toxic effects of aflatoxin B 1 (AFB 1 ) on mono-sex Nile tilapia Oreochromis niloticus fingerlings and attempting to detoxify these drastic effects by using some nutritious agents. Therefore, half percent of each of these nutritious agents namely Bio-Buds-2x, chamomile flowers, aspirin and ginger were added to aflatoxic (100 ppb aflatoxin B 1 ) diet for fingerlings. These diets were offered 6 days a week at 3% daily of actual biomass in glass aquaria in duplicate/treatment in an indoor feeding experiment lasted 14 weeks. The aflatoxic diet led to the worst fish growth performance and survival rate, feed and protein utilization, internal organs indices, carcass composition and residues of AFB 1 (ppb) in the whole body of fish and the tested parameters of blood haematology and biochemistry of the experimented fish. Dietary ginger inclusion alleviated aflatoxicosis symptoms by fish, since it improved all the above tested parameters of aflatoixcated fish. Generally, the obtained results in the present study indicated that the ginger was the best detoxifying agent of aflatoxin, followed by aspirin and chamomile flowers respectively.
... fish was measured biweekly to point feed quantity and to calculate growth performance accordi... more ... fish was measured biweekly to point feed quantity and to calculate growth performance according to Abdelhamid (2000) in form of: average weight gain (g/fish) AWG = average final weight (g) average initial weight (g), average daily gain, (g/fish/day) ADG = AWG ...