Dr. Mostafa F . El-Banna | Mansoura University (original) (raw)

Papers by Dr. Mostafa F . El-Banna

Research paper thumbnail of Exogenous application of proline mitigates deteriorative effects of salinity stress in NFT closed-loop system: An ultrastructural and physio-biochemical investigation on hydroponically grown tomato (Solanum lycopersicon L.)

Scientia horticulturae, Apr 1, 2024

Research paper thumbnail of Phytochemical, Anatomical, and Histochemical Investigation of Felicia abyssinica: A Chemical Analysis of Tissues in Relation to Their Structural Organization

Journal of Advanced Medical and Pharmaceutical Research (Online), Dec 25, 2023

Research paper thumbnail of Bee honey improved the performance of indole-3-butyric acid on promoting adventitious roots formation of Cupressus macrocarpa L. var. Goldcrest: Morpho-biochemical and histoanatomical investigation

Industrial Crops and Products, Feb 29, 2024

Research paper thumbnail of Alleviating chromium-induced oxidative stress in Vigna radiata through exogenous trehalose application: insights into growth, photosynthetic efficiency, mineral nutrient uptake, and reactive oxygen species scavenging enzyme activity enhancement

BMC plant biology, May 27, 2024

Research paper thumbnail of Effect of Garlic, Moringa and Licorice Extracts on Vegetative Growth and Chemical Constituents of Zanthoxylum beecheyanum K. Koch Plant

Journal of Plant Production, May 1, 2020

Two field experiments were conducted during (2017-2018) and (2018-2019) at a commercial Nursery (... more Two field experiments were conducted during (2017-2018) and (2018-2019) at a commercial Nursery (Abnaa Shaesha) of Dakahlia Governorate, Egypt to evaluate the effects of natural extracts of garlic, moringa, and licorice on enhancing vegetative growth and chemical constituents of Chinese pepper (Zanthoxylum beecheyanum K. Koch) plant. The concentrations of used natural extracts were 0, 5, 10 and 15 % of garlic and moringa extract and 0, 5, 10 and 15 g L-1 of licorice extract. These extracts were applied as a drench on the surface soil pots at 250 ml-1 pot from each extract twice monthly starting from May to September. Results indicated that treating Chinese pepper plants with garlic extract at 15 % recorded pronounced significant values in vegetative growth parameters such as number of branches, fresh and dry weight of leaves and branches as well as total alkaloids and phosphors % while, total chlorophyll, carotenoids, and total sugars were heights in response to garlic extract at 5 %. In addition, the superior plant contents from nitrogen and potassium % in the leaves were generally increased with the exogenous application of moringa extract at 5 %. Based on the obtained results, it could be concluded that applied of garlic extract at 15 % followed by 5 % gave the best vegetative growth and alkaloids percentage in Zanthoxylum beecheyanum plants under experiment condition.

Research paper thumbnail of EFFECT OF ASCORBIC, HUMIC AND NICOTINIC ACIDS ON CERTAIN BIOCHEMICL CONSTITUENTS IN FENUGREEK (Trigonella foenum-graecum L.)

Journal of Plant Production, Aug 1, 2015

Two field experiments were conducted to evaluate the effects of ascorbic (AsA), humic (HA) and ni... more Two field experiments were conducted to evaluate the effects of ascorbic (AsA), humic (HA) and nicotinic (NA) acid on certain biochemical constituents that influence seed quality of Fenugreek (Trigonella foenum-graecum L.). Results indicated that presoaking or presoaking plus spraying in by AsA, HA and NA increased flavonoids, alkaloids, trigonelline, protein, total carbohydrates and oil percentage in seeds. With all tested metabolic inducers (MI), the enhancing effect was more pronounced at its higher adopted level and when it was applied as a combined presoaking plus a foliar spray treatment. Total alkaloid percentage was highest in response to HA at 3000 mg/l whereas trigonelline percentage recorded highest value in response to AsA at 200 mg/l. On the other hand, total phenols percentage was generally decreased with exogenous application of all MI, and the reduction was more evident at the higher level of either AsA, HA or NA. Based on the obtained results, it could be concluded that metabolism of Fenugreek (Trigonella foenum-graecum L.) could be modulated toward the accumulation of beneficial components by exogenous application of AsA, HA, and NA.

Research paper thumbnail of Evaluation berries shattering phenomena of ‘Flame seedless’ vines grafted on different rootstocks during shelf life

Scientia Horticulturae, Feb 1, 2019

Grapevines are deciduous plants cultivated in different regions of the world, which is considered... more Grapevines are deciduous plants cultivated in different regions of the world, which is considered to have a large international economic return. The investigation was directed in two seasons 2017 and 2018 on vines 12-yearold in a commercial farm in sandy soil. The effect of grafting vines on four rootstocks 'Freedom', 'Ruggeri 140Ru', ' Paulson 1103 P', and 'Flame seedless' was assessed. Bunches samples were tested by determining SSC (16%) to determine the harvest date and stored at room temperature at (25 ± 1oC and 43 ± 1 air RH) for four days. Paulson 1103 P rootstock displays less berry shattering rate contrasted with different rootstocks. Additionally, the decrease in the activity of cellular walls hydrolysis enzymes, for example, Polygalacturonase (PG; EC:3.2.1.15), xylanase (XLN; EC:3.2.1.8), and cellulase (CEL; EC:3.2.1.4) was minimized at the low-level associated to other different grafted rootstocks. In addition, Paulson 1103 P rootstock shows also a superior impact on the physical and chemical quality characteristics during four days of the timing shelf life. Additionally, the decrease in the activity of cellular walls hydrolysis enzymes, for example, Polygalacturonase (PG; EC:3.2.1.15), xylanase (XLN; EC:3.2.1.8), and cellulase (CEL; EC:3.2.1.4) was minimized at the low-level associated to other different grafted rootstocks. In addition, Paulson 1103 P rootstock shows an obvious impact on the physical and chemical quality characteristics during four days of the shelf life time. The obtained results indicate that the fruit cluster behavior of 'Flame seedless vines was grafted on Paulson 1103 P rootstock present more reduction in berry shattering rate until to an end of the experimental storage (four days) at ambient air.

Research paper thumbnail of The Role of Grain Priming and its Duration on Wheat Germination and Seedling Growth

Journal of Plant Production, Apr 3, 2019

This study was designed to investigate the effect of different grain priming methods on germinati... more This study was designed to investigate the effect of different grain priming methods on germination and seedling vigour of wheat cultivar (Gemmiza 9) was performed. Grains were primed for 8, 16 and 24 hours in 13 priming media (water, polyethylene glycol 6000 at 2.5, 5, 7.5 %; CaCl 2 at 250, 500, 1000 ppm; ascorbic acid (AsA) at 25, 50, 100 ppm; salicylic acid (SA) at 50, 100, 150 ppm) beside dry grain as a control. In general, it was observed that a priming duration of 24 hr gave the highest values of all duration studied. In addition, results indicated that all grain priming in any priming materials significantly increased seedling vigour index (SVI) represented by germination %, length of seedling and fresh and dry weights of seedling. In most cases, SA at 100 ppm was the most effective in this regard. The greatest germination % was obtained due to 7.5 % PEG; 500 ppm CaCl 2 ; 100 ppm AsA and 100 ppm SA. The highest seedling length was obtained due to 2.5 % PEG; 250 ppm CaCl 2 ; 50 ppm AsA and 100 ppm SA. In addition, seedling dry weight was highest due to 7.5 % PEG; 1000 ppm CaCl 2 ; 50 ppm AsA and 100 ppm SA. The highest SVI values were obtained due to 2.5 % PEG; 250 ppm CaCl 2 ; 50 ppm AsA and 100 ppm SA. These results indicate that priming of grains had a significant effect to promote the germination and seedlings growth of wheat.

Research paper thumbnail of Interactive Effects of Certain Vitamins, Bioregulator and Yeast Extract on Sweet Pepper Stem and Leaf Anatomy Under Two Types of Salinity

Journal of Plant Production, Sep 1, 2013

All salinity types at 2000 mg/L increased stem diameter due to an increase in pith diameter, cort... more All salinity types at 2000 mg/L increased stem diameter due to an increase in pith diameter, cortex thickness, width of epidermis cell and vascular bundles dimensions (length as well as metaxylem vessel diameter). In addition, CaCl2 and NaCl+CaCl2 1:1 (w/w) were the most effective in this respect. In addition, high salinity level (4000 mg/L) decreased most of the studied anatomical parameters. While, the pith diameter and number of vascular bundles were decreased only under NaCl at 4000 mg/L. On the other hand, pre-soaking seeds in selected chemicals used, in most cases, showed a positive effect on the stem structure and AsA at 50 mg/L or SA at 75 mg/L was the most effective in this respect. Low level of all salinity types (2000 mg/L) increased midrib region thickness due to increasing the length of main vascular bundle. While, the highest salinity level (4000 mg/L) led to a decrease in this respect due to the decrease in length of main vascular bundle. In addition, NaCl was more effective in this respect followed by NaCl+CaCl2 (1:1). On the other hand, the leaf blade (lamina) thickness was also decreased in plants grown under NaCl at 4000 mg/L followed by NaCl+CaCl2 (1:1) due to a decrease in the thickness of palisade and spongy tissues as well as upper and lower epidermis width. Moreover, the application of chemicals used led to an increase in the thickness of midrib region as compared with untreated plants. In addition, SA (75 mg/L), AsA (50 mg/L) and α-tocopherol (100 mg/L) were more effective. In most cases, AsA at 50 mg/L or SA at 75 mg/L alleviated the harmful effect of salinity level (4000 mg/L) on midrib region and lamina thickness as well as the main vascular bundle dimensions when compared with untreated plants. Furthermore, AsA at 50 mg/L was more effective than the remaining treatments.

Research paper thumbnail of OVERCOMING THE HARMFUL EFFECTS OF NaCl AND CaCl2 SALINITY ON SWEET PEPPER PLANT GROWTH BY USING CERTAIN VITAMINS, BIO-REGULATOR AND YEAST EXTRACT

Journal of Plant Production, Sep 1, 2013

An experiment was conducted to study the influence of soaking the seeds of sweet pepper plant in ... more An experiment was conducted to study the influence of soaking the seeds of sweet pepper plant in selected chemicals used i.e. bio-regulator (salicylic acid), vitamins (ascorbic acid or α-tocopherol) or yeast extract on sweet pepper plant growth under non-saline or saline conditions. Low salinity level (2000 mg/L) in most cases, increased significantly sweet pepper growth parameters (plant height, root length, shoot and root fresh as well as dry weights, number of leaves as well as leaf area) after 75 and 90 days from sowing. In the contrast, increasing salinity levels decreased growth parameters. Whereas, NaCl+CaCl2 (1:1) were more effective followed by CaCl2 and NaCl.

Research paper thumbnail of Mineral Content in the Leaves of Sweet Pepper Plant in Response to Certain Bio-Stimulants Under Salt Stress Conditions

Journal of Plant Production, Apr 1, 2014

N, P, K+ and Mg2+ concentration as well as K+/Na+ ratio in sweet pepper root, shoot, and fruits w... more N, P, K+ and Mg2+ concentration as well as K+/Na+ ratio in sweet pepper root, shoot, and fruits were significantly increased at the low salinity level NaCl+CaCl2 (2000 mg/L) followed by CaCl2. In addition, increasing salinity levels led to a decrease in this respect. Meanwhile, CaCl2 or NaCl+CaCl2 increased significantly the Ca2+ concentration with increasing salinity levels from 2000 to 4000 mg/L as compared to control. On the other hand, calcium concentration was decreased significantly with increasing NaCl salinity level to 4000 mg/L. With increasing salinity levels, both Na+ and Cl-concentration were increased as compared with control. The highest value of Na+ was obtained by NaCl followed by NaCl+CaCl2. In addition, pre-soaking seeds in SA, AsA, α-tocopherol and yeast extract at both levels significantly increased N, P, K+, Ca2+ and Mg2+ concentrations as well as K+/Na+ ratio whereas decreased Na+ and Cl-under non-saline and saline conditions. In addition, AsA at 50 mg/L and SA at 75 mg/L were more effective as compared with the other treatments.  FAO: Food and Agriculture Organization of the united nation, Statistical agricultural database sector. www.http:// faostat.fao.org/site/567/ Substance Formula Weight Potassium dihydrogen Phosphate KH2PO4 263 Potassium Nitrate KNO3 583 Calcium Nitrate Ca(NO3)2. 4H2O 1003 Magnesium Sulphate MgSO4. 7H2O 513 EDTA Iron CH2.N(CH2.COO)2[2 Fe Na 79.

Research paper thumbnail of Response of Yield and Its Quality of Sweet Pepper Plant to Certain Bio-Stimulants Under Two Types of Salinity

Journal of advances in natural sciences, Oct 10, 2015

Number of fruits and its fresh weight per plant were significantly increased under low salinity l... more Number of fruits and its fresh weight per plant were significantly increased under low salinity level of NaCl+CaCl2 in the early 4th picking and total yield. While, plants grown under NaCl salinity did not produced fruits in the early 4th picking. However, increasing salinity levels caused a significant decrease in this respect. In addition, pre-soaking seeds in selected chemicals used at both levels caused a significant increase in sweet pepper fruit number and its fresh weight per plant (early 4th picking and total yield) under non-saline conditions. Moreover, salicylic acid at 75 mg/L or ascorbic acid at 50 mg/L was more effective in this respect under non-saline or salinity conditions. Ascorbic acid as well as total carbohydrates concentrations in sweet pepper fruits were increased significantly with increasing salinity levels. In addition, ascorbic acid and total carbohydrates concentrations were greatly increased in plants growing under NaCl followed by NaCl+CaCl2 and CaCl2 as compared to control. On the other hand, pre-soaking seeds in SA, AsA, α-tocopherol and yeast extract at both levels increased ascorbic acid and total carbohydrates concentrations under non-saline or salinity conditions. Furthermore, AsA at both levels or SA at 75 mg/L was more effective in this respect. Total soluble solids (TSS %) were significantly increased under low salinity level (2000 mg/L), thereafter decreased with increasing salinity level to 4000 mg/L. In addition, the great reduction in TSS % occurred under NaCl at high salinity levels as compared with the other salinity type. Moreover, pre-soaking seeds in chemicals used at both levels increased TSS % under non-saline or saline conditions. Furthermore, SA at 75 mg/L or AsA at 50 mg/L was more effective in this respect.

Research paper thumbnail of Response of Certain Phytochemical Constituents in Sweet Pepper Leaves to Some Bio-Stimulants Under Two Types of Salinity

Journal of advances in natural sciences, Oct 10, 2015

High salinity level of NaCl and CaCl2 (4000 mg/L) markedly increased whereas the low levels and i... more High salinity level of NaCl and CaCl2 (4000 mg/L) markedly increased whereas the low levels and its combination decreased electrolyte leakage percentage (E.L. %) in sweet pepper leaves as compared to the unstressed plants. In addition, application of all selected bio-regulators used at both applied levels, in most cases, alleviated the harmful effect of salinity on E.L. % especially ascorbic acid at 50 mg/L. Relative water content (RWC) and photosynthetic pigments were significantly increased thereafter decreased as salinity levels increased. In addition, CaCl2 at low level caused a great increase in RWC % followed by NaCl+CaCl2 and NaCl (2000 mg/L). While, pre-soaking seeds in both levels of the applied bio-stimulants caused a significant increase in RWC % and the photosynthetic pigments concentrations. In addition, AsA at 50 mg/L or SA at 75 mg/L was more effective in this respect as compared to the untreated plants. Ascorbic acid, proline, total phenols as well as total soluble carbohydrates concentrations in sweet pepper shoot were increased with increasing salinity levels from 2000 to 4000 mg/L of all salinity types. In addition, NaCl led to a great increase followed by NaCl+CaCl2 and CaCl2 as compared to the unstressed plants. In addition, pre-soaking seeds in SA, AsA, α-tocopherol and yeast extract at both levels increased ascorbic acid, proline, total phenols as well as total soluble carbohydrates concentrations under saline conditions. Moreover, AsA at 50 mg/L or SA at 75 mg/L was more effective in this respect as compared with the other treatments.

Research paper thumbnail of Anatomical Study on Watermelon Grafting

Journal of Plant Production, Oct 1, 2017

The present study was carried out at Al-Amana nursery in Talkha district, Dakahlya Governorate, E... more The present study was carried out at Al-Amana nursery in Talkha district, Dakahlya Governorate, Egypt, during the summer season of 2014 to study the effects of different graft combinations between rootstocks and scion of watermelon (Citrullus lanatus (Thunb.) Matsum et Nakai) var. Lanatus cv. Aswan F 1. The experiment included thirteen treatments resulted from the combinations of four rootstocks [Jumbo F 1 and Nun 6001 F 1 hybrids [Cucurbita maxima (Duch.) ex. Lam.× Cucurbita moschata (Duch.) ex. Poir.], bottle gourd (Lagenaria siceraria Standl.) and pumpkin (Cucurbita moschata L.)] and three grafting methods [Hole insertion (HIG), Splice (SG) and Tongue approach (TAG)] as well as non-grafted plants as control. The obtained results illustrated that there was a high compatibility between Watermelon(scion) and Jumbo followed by bottle gourd then Nun 6001 rootstock, where these graft combinations recorded the highest vascular thickness and area of vascular cambium compared to other graft combinations. Although, SG gave the highest area of graft union. In addition, TAG showed the greatest width of graft union which is related to increase of xylem and phloem tissues thickness and area of vascular cambium. Moreover, grafted plants onto Jumbo and bottle gourd using TAG method recorded the highest survival percentage (100 and 98%, respectively) in comparison with other graft combinations. While, grafting onto pumpkin rootstock using HIG method had the lowest values of all studies traits in comparison with other combinations. Generally, the callus development and graft success in Watermeloncv. Aswan F 1 can be performed using Jumbo rootstock with TAG followed by SG method.

Research paper thumbnail of Stomatal Density in the Leaves of Sweet Pepper Plant as Affected by Certain Bio-Stimulants Under Salt Stress Conditions

Journal of Plant Production, Apr 1, 2014

Increasing salinity levels of the two applied types decreased the number of stomata and its densi... more Increasing salinity levels of the two applied types decreased the number of stomata and its density on both surfaces of sweet pepper leaves as compared with control. In addition, NaCl at 4000 mg/L was more effective in this respect followed by NaCl+CaCl2 and CaCl2. On the other hand, pre-soaking sweet pepper seeds in AsA at 50 mg/L or SA at 75 mg/L increased the number of stomata and its density on both leaf surfaces as compared to control and such salinity levels. Moreover, AsA at 50 mg/L proved to be more affected than SA treatment in this respect.

Research paper thumbnail of EFFECT OF NaCl AND CaCl2 SALTS ON SEED GERMINATION AND SEEDLING GROWTH OF Capsicum annuum L

Journal of Plant Production, 2010

To compare the effect of NaCl, CaCl2 and their combinations on germination and early seedling gro... more To compare the effect of NaCl, CaCl2 and their combinations on germination and early seedling growth stages of Capsicum annuum L., two-separated experiments were laid out at Laboratory of the Agric. Botany Dept., Fac. of Agric., Mansoura Univ., Egypt, during the growing season 2008. Results indicated that significant increases were recorded in percentage of germination(GP), germination performance index (GPI), as well as seedling fresh and dry weights, seedling length, water content, catalase (CAT) activity and photosynthetic pigments concentrations (chlorophyll a, b and total chlorophylls as well as carotenoids) under the low level (2000 ppm) of NaCl or CaCl2 and their combination(1:1). Increasing salt concentration in nutrient cooper solution caused significant decrease in all of these parameters. The great reduction occurred under high salinity level of NaCl (4000 ppm). However, seedling water content (WC) was not-significantly decreased. In addition, CaCl2 at 4000 ppm increased significantly the total carotenoids as compared with NaCl. Meanwhile, peroxidase (POD) activity increased significantly with increasing salinity levels from 2000 to 4000 ppm of both applied salinity types. Moreover, POD activity under NaCl levels showed a marked increase followed by NaCl+ CaCl2 (1:1) and CaCl2 at 4000 ppm..

Research paper thumbnail of Pre and postharvest characteristics of Dahlia pinnata var. pinnata, cav. As affected by SiO2 and CaCO3 nanoparticles under two different planting dates

Research paper thumbnail of Exogenous IBA stimulated adventitious root formation of Zanthoxylum beecheyanum K. Koch stem cutting: Histo-physiological and phytohormonal investigation

Plant Physiology and Biochemistry

Research paper thumbnail of Anatomical and Physiological Performance of Jojoba Treated with Proline under Salinity Stress Condition

Horticulturae

A field trial study was conducted for two consecutive seasons 2020 and 2021 in approximately 8-mo... more A field trial study was conducted for two consecutive seasons 2020 and 2021 in approximately 8-month-old jojoba plants to evaluate the physiological responses following salt treatment and the role of proline as a foliar application to enhance jojoba tolerance to salinity stress. Jojoba plants were irrigated once a week for four months with diluted seawater in concentrations of 5000, 10,000, and 15,000 ppm and tap water (control). Anti-stress proline was applied four times throughout the experiment, the first at the beginning of the experiment and another three times at 30-day intervals, at concentrations of 0, 300, and 450 ppm. The effect of proline treatments on jojoba plant behavior includes growth vegetative characteristics, namely plant height increase percentage (PHIP), shoot number increase percentage (NSIP), stem diameter increase percentage (SDIP), number of leaves, leaf thickness, leaf area, and fresh and dry weights of leaves, and chemical characteristics, namely chlorophy...

Research paper thumbnail of Morpho-Physiological and Anatomical Alterations of Salt-Affected Thompson Seedless Grapevine (Vitis vinifera L.) to Brassinolide Spraying

Horticulturae

Salinity is one of the most critical crises worldwide that ultimately compromises future food sec... more Salinity is one of the most critical crises worldwide that ultimately compromises future food security. Brassinosteroids including brassinolide (BL) are a class of polyhydroxy steroids phytohormones, that play a crucial role in several plant metabolic pathways and boost plants’ stress tolerance, but less data is accessible on its function in salt-affected grapevine. The experiment was conducted throughout the 2019 and 2020 experimental seasons at EL-Baramon experimental farm, Horticulture Research Institute, Mansoura, Egypt, to recognize the remediation potential of BL (1 and 2 mg L−1) in lightening salinity (NaCl at 1000, 2000, and 3000 mg L−1) injury on Thompson seedless grapevine seedlings (H4 strain) growth and physio-anatomical attributes. Data advocated that while salinity reduced growth attributes, BL applications substantially improved the overall salt-affected plant performance. Salinity stress significantly decreased photosynthetic pigment, relative water content, and ions...

Research paper thumbnail of Exogenous application of proline mitigates deteriorative effects of salinity stress in NFT closed-loop system: An ultrastructural and physio-biochemical investigation on hydroponically grown tomato (Solanum lycopersicon L.)

Scientia horticulturae, Apr 1, 2024

Research paper thumbnail of Phytochemical, Anatomical, and Histochemical Investigation of Felicia abyssinica: A Chemical Analysis of Tissues in Relation to Their Structural Organization

Journal of Advanced Medical and Pharmaceutical Research (Online), Dec 25, 2023

Research paper thumbnail of Bee honey improved the performance of indole-3-butyric acid on promoting adventitious roots formation of Cupressus macrocarpa L. var. Goldcrest: Morpho-biochemical and histoanatomical investigation

Industrial Crops and Products, Feb 29, 2024

Research paper thumbnail of Alleviating chromium-induced oxidative stress in Vigna radiata through exogenous trehalose application: insights into growth, photosynthetic efficiency, mineral nutrient uptake, and reactive oxygen species scavenging enzyme activity enhancement

BMC plant biology, May 27, 2024

Research paper thumbnail of Effect of Garlic, Moringa and Licorice Extracts on Vegetative Growth and Chemical Constituents of Zanthoxylum beecheyanum K. Koch Plant

Journal of Plant Production, May 1, 2020

Two field experiments were conducted during (2017-2018) and (2018-2019) at a commercial Nursery (... more Two field experiments were conducted during (2017-2018) and (2018-2019) at a commercial Nursery (Abnaa Shaesha) of Dakahlia Governorate, Egypt to evaluate the effects of natural extracts of garlic, moringa, and licorice on enhancing vegetative growth and chemical constituents of Chinese pepper (Zanthoxylum beecheyanum K. Koch) plant. The concentrations of used natural extracts were 0, 5, 10 and 15 % of garlic and moringa extract and 0, 5, 10 and 15 g L-1 of licorice extract. These extracts were applied as a drench on the surface soil pots at 250 ml-1 pot from each extract twice monthly starting from May to September. Results indicated that treating Chinese pepper plants with garlic extract at 15 % recorded pronounced significant values in vegetative growth parameters such as number of branches, fresh and dry weight of leaves and branches as well as total alkaloids and phosphors % while, total chlorophyll, carotenoids, and total sugars were heights in response to garlic extract at 5 %. In addition, the superior plant contents from nitrogen and potassium % in the leaves were generally increased with the exogenous application of moringa extract at 5 %. Based on the obtained results, it could be concluded that applied of garlic extract at 15 % followed by 5 % gave the best vegetative growth and alkaloids percentage in Zanthoxylum beecheyanum plants under experiment condition.

Research paper thumbnail of EFFECT OF ASCORBIC, HUMIC AND NICOTINIC ACIDS ON CERTAIN BIOCHEMICL CONSTITUENTS IN FENUGREEK (Trigonella foenum-graecum L.)

Journal of Plant Production, Aug 1, 2015

Two field experiments were conducted to evaluate the effects of ascorbic (AsA), humic (HA) and ni... more Two field experiments were conducted to evaluate the effects of ascorbic (AsA), humic (HA) and nicotinic (NA) acid on certain biochemical constituents that influence seed quality of Fenugreek (Trigonella foenum-graecum L.). Results indicated that presoaking or presoaking plus spraying in by AsA, HA and NA increased flavonoids, alkaloids, trigonelline, protein, total carbohydrates and oil percentage in seeds. With all tested metabolic inducers (MI), the enhancing effect was more pronounced at its higher adopted level and when it was applied as a combined presoaking plus a foliar spray treatment. Total alkaloid percentage was highest in response to HA at 3000 mg/l whereas trigonelline percentage recorded highest value in response to AsA at 200 mg/l. On the other hand, total phenols percentage was generally decreased with exogenous application of all MI, and the reduction was more evident at the higher level of either AsA, HA or NA. Based on the obtained results, it could be concluded that metabolism of Fenugreek (Trigonella foenum-graecum L.) could be modulated toward the accumulation of beneficial components by exogenous application of AsA, HA, and NA.

Research paper thumbnail of Evaluation berries shattering phenomena of ‘Flame seedless’ vines grafted on different rootstocks during shelf life

Scientia Horticulturae, Feb 1, 2019

Grapevines are deciduous plants cultivated in different regions of the world, which is considered... more Grapevines are deciduous plants cultivated in different regions of the world, which is considered to have a large international economic return. The investigation was directed in two seasons 2017 and 2018 on vines 12-yearold in a commercial farm in sandy soil. The effect of grafting vines on four rootstocks 'Freedom', 'Ruggeri 140Ru', ' Paulson 1103 P', and 'Flame seedless' was assessed. Bunches samples were tested by determining SSC (16%) to determine the harvest date and stored at room temperature at (25 ± 1oC and 43 ± 1 air RH) for four days. Paulson 1103 P rootstock displays less berry shattering rate contrasted with different rootstocks. Additionally, the decrease in the activity of cellular walls hydrolysis enzymes, for example, Polygalacturonase (PG; EC:3.2.1.15), xylanase (XLN; EC:3.2.1.8), and cellulase (CEL; EC:3.2.1.4) was minimized at the low-level associated to other different grafted rootstocks. In addition, Paulson 1103 P rootstock shows also a superior impact on the physical and chemical quality characteristics during four days of the timing shelf life. Additionally, the decrease in the activity of cellular walls hydrolysis enzymes, for example, Polygalacturonase (PG; EC:3.2.1.15), xylanase (XLN; EC:3.2.1.8), and cellulase (CEL; EC:3.2.1.4) was minimized at the low-level associated to other different grafted rootstocks. In addition, Paulson 1103 P rootstock shows an obvious impact on the physical and chemical quality characteristics during four days of the shelf life time. The obtained results indicate that the fruit cluster behavior of 'Flame seedless vines was grafted on Paulson 1103 P rootstock present more reduction in berry shattering rate until to an end of the experimental storage (four days) at ambient air.

Research paper thumbnail of The Role of Grain Priming and its Duration on Wheat Germination and Seedling Growth

Journal of Plant Production, Apr 3, 2019

This study was designed to investigate the effect of different grain priming methods on germinati... more This study was designed to investigate the effect of different grain priming methods on germination and seedling vigour of wheat cultivar (Gemmiza 9) was performed. Grains were primed for 8, 16 and 24 hours in 13 priming media (water, polyethylene glycol 6000 at 2.5, 5, 7.5 %; CaCl 2 at 250, 500, 1000 ppm; ascorbic acid (AsA) at 25, 50, 100 ppm; salicylic acid (SA) at 50, 100, 150 ppm) beside dry grain as a control. In general, it was observed that a priming duration of 24 hr gave the highest values of all duration studied. In addition, results indicated that all grain priming in any priming materials significantly increased seedling vigour index (SVI) represented by germination %, length of seedling and fresh and dry weights of seedling. In most cases, SA at 100 ppm was the most effective in this regard. The greatest germination % was obtained due to 7.5 % PEG; 500 ppm CaCl 2 ; 100 ppm AsA and 100 ppm SA. The highest seedling length was obtained due to 2.5 % PEG; 250 ppm CaCl 2 ; 50 ppm AsA and 100 ppm SA. In addition, seedling dry weight was highest due to 7.5 % PEG; 1000 ppm CaCl 2 ; 50 ppm AsA and 100 ppm SA. The highest SVI values were obtained due to 2.5 % PEG; 250 ppm CaCl 2 ; 50 ppm AsA and 100 ppm SA. These results indicate that priming of grains had a significant effect to promote the germination and seedlings growth of wheat.

Research paper thumbnail of Interactive Effects of Certain Vitamins, Bioregulator and Yeast Extract on Sweet Pepper Stem and Leaf Anatomy Under Two Types of Salinity

Journal of Plant Production, Sep 1, 2013

All salinity types at 2000 mg/L increased stem diameter due to an increase in pith diameter, cort... more All salinity types at 2000 mg/L increased stem diameter due to an increase in pith diameter, cortex thickness, width of epidermis cell and vascular bundles dimensions (length as well as metaxylem vessel diameter). In addition, CaCl2 and NaCl+CaCl2 1:1 (w/w) were the most effective in this respect. In addition, high salinity level (4000 mg/L) decreased most of the studied anatomical parameters. While, the pith diameter and number of vascular bundles were decreased only under NaCl at 4000 mg/L. On the other hand, pre-soaking seeds in selected chemicals used, in most cases, showed a positive effect on the stem structure and AsA at 50 mg/L or SA at 75 mg/L was the most effective in this respect. Low level of all salinity types (2000 mg/L) increased midrib region thickness due to increasing the length of main vascular bundle. While, the highest salinity level (4000 mg/L) led to a decrease in this respect due to the decrease in length of main vascular bundle. In addition, NaCl was more effective in this respect followed by NaCl+CaCl2 (1:1). On the other hand, the leaf blade (lamina) thickness was also decreased in plants grown under NaCl at 4000 mg/L followed by NaCl+CaCl2 (1:1) due to a decrease in the thickness of palisade and spongy tissues as well as upper and lower epidermis width. Moreover, the application of chemicals used led to an increase in the thickness of midrib region as compared with untreated plants. In addition, SA (75 mg/L), AsA (50 mg/L) and α-tocopherol (100 mg/L) were more effective. In most cases, AsA at 50 mg/L or SA at 75 mg/L alleviated the harmful effect of salinity level (4000 mg/L) on midrib region and lamina thickness as well as the main vascular bundle dimensions when compared with untreated plants. Furthermore, AsA at 50 mg/L was more effective than the remaining treatments.

Research paper thumbnail of OVERCOMING THE HARMFUL EFFECTS OF NaCl AND CaCl2 SALINITY ON SWEET PEPPER PLANT GROWTH BY USING CERTAIN VITAMINS, BIO-REGULATOR AND YEAST EXTRACT

Journal of Plant Production, Sep 1, 2013

An experiment was conducted to study the influence of soaking the seeds of sweet pepper plant in ... more An experiment was conducted to study the influence of soaking the seeds of sweet pepper plant in selected chemicals used i.e. bio-regulator (salicylic acid), vitamins (ascorbic acid or α-tocopherol) or yeast extract on sweet pepper plant growth under non-saline or saline conditions. Low salinity level (2000 mg/L) in most cases, increased significantly sweet pepper growth parameters (plant height, root length, shoot and root fresh as well as dry weights, number of leaves as well as leaf area) after 75 and 90 days from sowing. In the contrast, increasing salinity levels decreased growth parameters. Whereas, NaCl+CaCl2 (1:1) were more effective followed by CaCl2 and NaCl.

Research paper thumbnail of Mineral Content in the Leaves of Sweet Pepper Plant in Response to Certain Bio-Stimulants Under Salt Stress Conditions

Journal of Plant Production, Apr 1, 2014

N, P, K+ and Mg2+ concentration as well as K+/Na+ ratio in sweet pepper root, shoot, and fruits w... more N, P, K+ and Mg2+ concentration as well as K+/Na+ ratio in sweet pepper root, shoot, and fruits were significantly increased at the low salinity level NaCl+CaCl2 (2000 mg/L) followed by CaCl2. In addition, increasing salinity levels led to a decrease in this respect. Meanwhile, CaCl2 or NaCl+CaCl2 increased significantly the Ca2+ concentration with increasing salinity levels from 2000 to 4000 mg/L as compared to control. On the other hand, calcium concentration was decreased significantly with increasing NaCl salinity level to 4000 mg/L. With increasing salinity levels, both Na+ and Cl-concentration were increased as compared with control. The highest value of Na+ was obtained by NaCl followed by NaCl+CaCl2. In addition, pre-soaking seeds in SA, AsA, α-tocopherol and yeast extract at both levels significantly increased N, P, K+, Ca2+ and Mg2+ concentrations as well as K+/Na+ ratio whereas decreased Na+ and Cl-under non-saline and saline conditions. In addition, AsA at 50 mg/L and SA at 75 mg/L were more effective as compared with the other treatments.  FAO: Food and Agriculture Organization of the united nation, Statistical agricultural database sector. www.http:// faostat.fao.org/site/567/ Substance Formula Weight Potassium dihydrogen Phosphate KH2PO4 263 Potassium Nitrate KNO3 583 Calcium Nitrate Ca(NO3)2. 4H2O 1003 Magnesium Sulphate MgSO4. 7H2O 513 EDTA Iron CH2.N(CH2.COO)2[2 Fe Na 79.

Research paper thumbnail of Response of Yield and Its Quality of Sweet Pepper Plant to Certain Bio-Stimulants Under Two Types of Salinity

Journal of advances in natural sciences, Oct 10, 2015

Number of fruits and its fresh weight per plant were significantly increased under low salinity l... more Number of fruits and its fresh weight per plant were significantly increased under low salinity level of NaCl+CaCl2 in the early 4th picking and total yield. While, plants grown under NaCl salinity did not produced fruits in the early 4th picking. However, increasing salinity levels caused a significant decrease in this respect. In addition, pre-soaking seeds in selected chemicals used at both levels caused a significant increase in sweet pepper fruit number and its fresh weight per plant (early 4th picking and total yield) under non-saline conditions. Moreover, salicylic acid at 75 mg/L or ascorbic acid at 50 mg/L was more effective in this respect under non-saline or salinity conditions. Ascorbic acid as well as total carbohydrates concentrations in sweet pepper fruits were increased significantly with increasing salinity levels. In addition, ascorbic acid and total carbohydrates concentrations were greatly increased in plants growing under NaCl followed by NaCl+CaCl2 and CaCl2 as compared to control. On the other hand, pre-soaking seeds in SA, AsA, α-tocopherol and yeast extract at both levels increased ascorbic acid and total carbohydrates concentrations under non-saline or salinity conditions. Furthermore, AsA at both levels or SA at 75 mg/L was more effective in this respect. Total soluble solids (TSS %) were significantly increased under low salinity level (2000 mg/L), thereafter decreased with increasing salinity level to 4000 mg/L. In addition, the great reduction in TSS % occurred under NaCl at high salinity levels as compared with the other salinity type. Moreover, pre-soaking seeds in chemicals used at both levels increased TSS % under non-saline or saline conditions. Furthermore, SA at 75 mg/L or AsA at 50 mg/L was more effective in this respect.

Research paper thumbnail of Response of Certain Phytochemical Constituents in Sweet Pepper Leaves to Some Bio-Stimulants Under Two Types of Salinity

Journal of advances in natural sciences, Oct 10, 2015

High salinity level of NaCl and CaCl2 (4000 mg/L) markedly increased whereas the low levels and i... more High salinity level of NaCl and CaCl2 (4000 mg/L) markedly increased whereas the low levels and its combination decreased electrolyte leakage percentage (E.L. %) in sweet pepper leaves as compared to the unstressed plants. In addition, application of all selected bio-regulators used at both applied levels, in most cases, alleviated the harmful effect of salinity on E.L. % especially ascorbic acid at 50 mg/L. Relative water content (RWC) and photosynthetic pigments were significantly increased thereafter decreased as salinity levels increased. In addition, CaCl2 at low level caused a great increase in RWC % followed by NaCl+CaCl2 and NaCl (2000 mg/L). While, pre-soaking seeds in both levels of the applied bio-stimulants caused a significant increase in RWC % and the photosynthetic pigments concentrations. In addition, AsA at 50 mg/L or SA at 75 mg/L was more effective in this respect as compared to the untreated plants. Ascorbic acid, proline, total phenols as well as total soluble carbohydrates concentrations in sweet pepper shoot were increased with increasing salinity levels from 2000 to 4000 mg/L of all salinity types. In addition, NaCl led to a great increase followed by NaCl+CaCl2 and CaCl2 as compared to the unstressed plants. In addition, pre-soaking seeds in SA, AsA, α-tocopherol and yeast extract at both levels increased ascorbic acid, proline, total phenols as well as total soluble carbohydrates concentrations under saline conditions. Moreover, AsA at 50 mg/L or SA at 75 mg/L was more effective in this respect as compared with the other treatments.

Research paper thumbnail of Anatomical Study on Watermelon Grafting

Journal of Plant Production, Oct 1, 2017

The present study was carried out at Al-Amana nursery in Talkha district, Dakahlya Governorate, E... more The present study was carried out at Al-Amana nursery in Talkha district, Dakahlya Governorate, Egypt, during the summer season of 2014 to study the effects of different graft combinations between rootstocks and scion of watermelon (Citrullus lanatus (Thunb.) Matsum et Nakai) var. Lanatus cv. Aswan F 1. The experiment included thirteen treatments resulted from the combinations of four rootstocks [Jumbo F 1 and Nun 6001 F 1 hybrids [Cucurbita maxima (Duch.) ex. Lam.× Cucurbita moschata (Duch.) ex. Poir.], bottle gourd (Lagenaria siceraria Standl.) and pumpkin (Cucurbita moschata L.)] and three grafting methods [Hole insertion (HIG), Splice (SG) and Tongue approach (TAG)] as well as non-grafted plants as control. The obtained results illustrated that there was a high compatibility between Watermelon(scion) and Jumbo followed by bottle gourd then Nun 6001 rootstock, where these graft combinations recorded the highest vascular thickness and area of vascular cambium compared to other graft combinations. Although, SG gave the highest area of graft union. In addition, TAG showed the greatest width of graft union which is related to increase of xylem and phloem tissues thickness and area of vascular cambium. Moreover, grafted plants onto Jumbo and bottle gourd using TAG method recorded the highest survival percentage (100 and 98%, respectively) in comparison with other graft combinations. While, grafting onto pumpkin rootstock using HIG method had the lowest values of all studies traits in comparison with other combinations. Generally, the callus development and graft success in Watermeloncv. Aswan F 1 can be performed using Jumbo rootstock with TAG followed by SG method.

Research paper thumbnail of Stomatal Density in the Leaves of Sweet Pepper Plant as Affected by Certain Bio-Stimulants Under Salt Stress Conditions

Journal of Plant Production, Apr 1, 2014

Increasing salinity levels of the two applied types decreased the number of stomata and its densi... more Increasing salinity levels of the two applied types decreased the number of stomata and its density on both surfaces of sweet pepper leaves as compared with control. In addition, NaCl at 4000 mg/L was more effective in this respect followed by NaCl+CaCl2 and CaCl2. On the other hand, pre-soaking sweet pepper seeds in AsA at 50 mg/L or SA at 75 mg/L increased the number of stomata and its density on both leaf surfaces as compared to control and such salinity levels. Moreover, AsA at 50 mg/L proved to be more affected than SA treatment in this respect.

Research paper thumbnail of EFFECT OF NaCl AND CaCl2 SALTS ON SEED GERMINATION AND SEEDLING GROWTH OF Capsicum annuum L

Journal of Plant Production, 2010

To compare the effect of NaCl, CaCl2 and their combinations on germination and early seedling gro... more To compare the effect of NaCl, CaCl2 and their combinations on germination and early seedling growth stages of Capsicum annuum L., two-separated experiments were laid out at Laboratory of the Agric. Botany Dept., Fac. of Agric., Mansoura Univ., Egypt, during the growing season 2008. Results indicated that significant increases were recorded in percentage of germination(GP), germination performance index (GPI), as well as seedling fresh and dry weights, seedling length, water content, catalase (CAT) activity and photosynthetic pigments concentrations (chlorophyll a, b and total chlorophylls as well as carotenoids) under the low level (2000 ppm) of NaCl or CaCl2 and their combination(1:1). Increasing salt concentration in nutrient cooper solution caused significant decrease in all of these parameters. The great reduction occurred under high salinity level of NaCl (4000 ppm). However, seedling water content (WC) was not-significantly decreased. In addition, CaCl2 at 4000 ppm increased significantly the total carotenoids as compared with NaCl. Meanwhile, peroxidase (POD) activity increased significantly with increasing salinity levels from 2000 to 4000 ppm of both applied salinity types. Moreover, POD activity under NaCl levels showed a marked increase followed by NaCl+ CaCl2 (1:1) and CaCl2 at 4000 ppm..

Research paper thumbnail of Pre and postharvest characteristics of Dahlia pinnata var. pinnata, cav. As affected by SiO2 and CaCO3 nanoparticles under two different planting dates

Research paper thumbnail of Exogenous IBA stimulated adventitious root formation of Zanthoxylum beecheyanum K. Koch stem cutting: Histo-physiological and phytohormonal investigation

Plant Physiology and Biochemistry

Research paper thumbnail of Anatomical and Physiological Performance of Jojoba Treated with Proline under Salinity Stress Condition

Horticulturae

A field trial study was conducted for two consecutive seasons 2020 and 2021 in approximately 8-mo... more A field trial study was conducted for two consecutive seasons 2020 and 2021 in approximately 8-month-old jojoba plants to evaluate the physiological responses following salt treatment and the role of proline as a foliar application to enhance jojoba tolerance to salinity stress. Jojoba plants were irrigated once a week for four months with diluted seawater in concentrations of 5000, 10,000, and 15,000 ppm and tap water (control). Anti-stress proline was applied four times throughout the experiment, the first at the beginning of the experiment and another three times at 30-day intervals, at concentrations of 0, 300, and 450 ppm. The effect of proline treatments on jojoba plant behavior includes growth vegetative characteristics, namely plant height increase percentage (PHIP), shoot number increase percentage (NSIP), stem diameter increase percentage (SDIP), number of leaves, leaf thickness, leaf area, and fresh and dry weights of leaves, and chemical characteristics, namely chlorophy...

Research paper thumbnail of Morpho-Physiological and Anatomical Alterations of Salt-Affected Thompson Seedless Grapevine (Vitis vinifera L.) to Brassinolide Spraying

Horticulturae

Salinity is one of the most critical crises worldwide that ultimately compromises future food sec... more Salinity is one of the most critical crises worldwide that ultimately compromises future food security. Brassinosteroids including brassinolide (BL) are a class of polyhydroxy steroids phytohormones, that play a crucial role in several plant metabolic pathways and boost plants’ stress tolerance, but less data is accessible on its function in salt-affected grapevine. The experiment was conducted throughout the 2019 and 2020 experimental seasons at EL-Baramon experimental farm, Horticulture Research Institute, Mansoura, Egypt, to recognize the remediation potential of BL (1 and 2 mg L−1) in lightening salinity (NaCl at 1000, 2000, and 3000 mg L−1) injury on Thompson seedless grapevine seedlings (H4 strain) growth and physio-anatomical attributes. Data advocated that while salinity reduced growth attributes, BL applications substantially improved the overall salt-affected plant performance. Salinity stress significantly decreased photosynthetic pigment, relative water content, and ions...