Salah Aref | Mansoura University (original) (raw)

Papers by Salah Aref

Hematology, 2006

Background: Disorders of the cell cycle regulatory machinery play a key role in the pathogenesis ... more Background: Disorders of the cell cycle regulatory machinery play a key role in the pathogenesis of cancer. Over expression of cyclin D1 protein has been reported in several solid tumors and certain lymphoid malignancies, but little is known about the involvement of cyclin D1 in ...

Pediatric Hematology and Oncology

This study aimed to address the clinical impact of recombinationactivating gene (RAG1) expression... more This study aimed to address the clinical impact of recombinationactivating gene (RAG1) expression and tumor suppressor IKZF1 gene deletions in Philadelphia negative B-cell precursor acute lymphoblastic leukemia (B-ALL) patients. Fifty newly diagnosed pediatric Philadelphia negative BALL patients were included in this study. Using Bone Marrow samples, RAG1 expression was assessed by real time PCR and IKZF1 deletions were determined by multiplex realtime quantitative PCR. The expression of RAG1 was significantly higher in BALL patients as compared to the controls (p < .001). The BALL patients with RAG1 high expression (median) had lower response to induction of remission, shorter DFS, shorter overall survival, higher blast cells, and white cell counts in the peripheral blood as compared to those with low RAG1 expression levels (p < .01 for all). Likewise, there was significant association between IKZF1 deletion and high RAG1 expression. Based on our findings RAG1 high expression and IKZF1 deletions were associated with adverse prognosis in Philadelphia negative BALL. RAG1 could be used as therapeutic target in the treatment of BALL .

Pediatric Hematology and Oncology

This study aimed to address the clinical impact of recombinationactivating gene (RAG1) expression... more This study aimed to address the clinical impact of recombinationactivating gene (RAG1) expression and tumor suppressor IKZF1 gene deletions in Philadelphia negative B-cell precursor acute lymphoblastic leukemia (B-ALL) patients. Fifty newly diagnosed pediatric Philadelphia negative BALL patients were included in this study. Using Bone Marrow samples, RAG1 expression was assessed by real time PCR and IKZF1 deletions were determined by multiplex realtime quantitative PCR. The expression of RAG1 was significantly higher in BALL patients as compared to the controls (p < .001). The BALL patients with RAG1 high expression (median) had lower response to induction of remission, shorter DFS, shorter overall survival, higher blast cells, and white cell counts in the peripheral blood as compared to those with low RAG1 expression levels (p < .01 for all). Likewise, there was significant association between IKZF1 deletion and high RAG1 expression. Based on our findings RAG1 high expression and IKZF1 deletions were associated with adverse prognosis in Philadelphia negative BALL. RAG1 could be used as therapeutic target in the treatment of BALL .

Asian Pacific journal of cancer prevention : APJCP, 2021

BACKGROUND Bone marrow myelofibrosis (BMF) that develop on top of Polycythaemia vera (PV) and ess... more BACKGROUND Bone marrow myelofibrosis (BMF) that develop on top of Polycythaemia vera (PV) and essential thrombocythemia leads to shortening of the patient's overall survival. This study aimed to address the impact of miR-146a rs2431697 polymorphism on inflammatory biomarkers and genes expression and the hazards of myelofibrosis progression. PATIENTS AND METHODS The study included 88 myeloproliferative neoplasm (40 PV; 27 ET; 21 MF) and 90 healthy controls. For all investigated subjects miR-146a rs2431697 genotypes were identified by sequencing and the expression of miR-146a; IL-1β; NF-κB; a NOD-like receptor family, pyrin domain containing 3 (NLRP3) (NLRP3) genes were estimated by real time PCR. RESULTS miR146a genotypes revealed that there was significant association between TT and TC genotypes with MF. The degree of miR146a expression was significantly reduced in MF as compared to both PV and ET. In contrast; the levels of IL-1β; NF-κB; NLRP3 genes expression were significantl...

Journal of Pediatric Hematology/Oncology, 2022

INTRODUCTION Pediatric immune thrombocytopenia (ITP) is a potentially life threating autoimmune d... more INTRODUCTION Pediatric immune thrombocytopenia (ITP) is a potentially life threating autoimmune disorder with different responses to therapy and different bleeding phenotypes in critical organs. The molecular basis for the variable response has not yet been fully elucidated. This study was designed to address the predictive value of regulatory B-cell (Breg) count and interleukin-10 (IL-10) serum levels for acute ITP patients who progress to chronic phase. The present study included 80 children with acute ITP )38 males and 42 females (with median age of 8 years and 40 matched healthy controls. Assessment of Breg (CD19+CD24hiCD38hi) was carried out by a multicolor flowcytometry, however, IL-10 serum levels were evaluated by enzyme-linked immunosorbent assay. A significant reduction of Breg percentage and a significant increase in serum IL-10 levels were identified in children with acute ITP as compared with controls (P<0.001 for both). Fourteen ITP patients passed to chronic phase, while 66 patients achieved remission within 6 months. The absolute Breg was significantly lower, while IL-10 was significantly higher in patients with acute ITP who progressed to chronic phase in comparison with acute ITP patients who achieved complete remission. Cox proportional hazards for ITP chronicity revealed that IL-10 OR was 2.46 (confidence interval: 1.42-4.27; P=0.001) and absolute Breg OR was 0.147 (confidence interval: 0.128-0.624; P=0.005) in the peripheral blood. Therefore, they could predict chronicity in ITP cases. CONCLUSION Reduced Breg count and elevated IL-10 levels in patients with acute ITP at diagnosis can predict chronicity.

Asian Pacific Journal of Cancer Prevention, 2021

Background: Recent reports suggested relation between Interferon Gamma (IFN-γ) gene polymorphism ... more Background: Recent reports suggested relation between Interferon Gamma (IFN-γ) gene polymorphism and the risk of development of HCC on top of hepatic cirrhosis. The aim of this study was to address the predictive value of Interferon Gamma gene receptor (IFN-γR) polymorphisms for the occurrence of hepatocellular carcinoma on top of liver cirrhosis. Patients and Methods: This is a case control study performed on patients selected from the outpatient hepatology clinic, specialized medical hospital, Mansoura University, Egypt, from August 2017 to February 2019. The included patients were categorized into two groups; 60 patients with HCC on top of cirrhosis and 20 patients with hepatic cirrhosis. For all patients IFN-γR polymorphism was identified by RFLP. Results: Our study showed that HCC patients had male predominance. Additionally, diabetes mellitus (DM) was found in 28.3% of total HCC patients. Half of HCC patients in this study were from rural areas (50%). The frequency of AA at position-611 in the IFN-γR (-611 IFN-γR) was significantly higher in the HCC group as compared to cirrhotic group (P=0.021). Moreover; the frequency of CC and CT genotypes of IFN-γR-56 was not significantly different in HCC group as compared to control group (P>0.05). The IFN-γR (-611 IFN-γ) AA genotype significantly increased risk of HCC (OR= 0.78, 95% CI= 0.10-6.39; P= 0.042). Conclusion: The analysis of IFN-γR-611 single nucleotide gene polymorphism could be a valuable marker for predicting subgroup of cirrhotic patients with high risk of developing HCC. Cirrhotic patients have AA genotype of IFN-γR-611 recommended to be under close follow up.

Clinical Lymphoma Myeloma and Leukemia, 2021

This study aimed to address the prognostic relevance of CD34+/CD38-/TIM3+ leukemic stem cell (LSC... more This study aimed to address the prognostic relevance of CD34+/CD38-/TIM3+ leukemic stem cell (LSC) frequency in patients with acute myeloid leukemia (AML) and its impact on patient outcome. We analyzed the expression of LSC markers (CD34+/CD38-/TIM3+) using flow cytometry in bone marrow samples of 53 AML cases before and after induction chemotherapy. The LSC frequency at diagnosis was significantly higher compared with that postinduction (P < .001). Patients were categorized into high LSC expressers (≥ median) and low expressers (< median). Patients with AML with high number of LSCs at diagnosis had significantly lower induction of remission response (P = .0104), shorter disease-free survival, and shorter overall survival (P < .001 for both) compared with those with lower LSC count. Cox regression analysis revealed that LSC frequency at diagnosis is an independent prognostic factor in AML. Assessment of LSCs (CD34+/CD38-/TIM3+) at diagnosis is recommended for refining of AML risk stratification.

Clinical Lymphoma Myeloma and Leukemia, 2021

Asian Pacific Journal of Cancer Prevention : APJCP, 2021

Background: Transducin-like enhancer of split 1 (TLE1( is a member of the TLE family of transcrip... more Background: Transducin-like enhancer of split 1 (TLE1( is a member of the TLE family of transcriptional co-repressors that control the transcription of a wide range of genes. The aim of this study was to evaluate the prognostic role of TLE1 gene expression in patients with T-cell acute lymphoblastic leukemia (T-ALL). Method: This study was conducted on 97 newly diagnosed T-ALL patients admitted to the Mansoura University oncology center (59 males and 38 females) with median age (33 years) in addition to 102 apparently healthy individuals served as a control group. TLE1 gene expression was measured in both patients and control groups by real time – PCR. The calculation of relative gene expression was done using the ΔΔCt method. Results: TEL1 gene expression was significantly down regulated in T-ALL cases (median 2.83) as compared to controls (median 84.65) (p < 0.001). The low TEL1 gene expression was significantly associated with CNS infiltration, non-remission and higher relapse...

The consultants Meeting on Cancer Medicine, Radiology & Treatment 2020 is planned to be conducted... more The consultants Meeting on Cancer Medicine, Radiology & Treatment 2020 is planned to be conducted within the month of Gregorian calendar month 29-30, 2020 at Athens, Greece. This Conference is CME & CPD authorized. Cancer meeting summit 2020 are going to be organized around the theme “Revolutions in Cancer Prevention and Diagnosis”.

Cancer Biomarkers, 2021

BACKGROUND: Acute myeloid leukemia changes the bone marrow (BM) niche to support leukemia cells b... more BACKGROUND: Acute myeloid leukemia changes the bone marrow (BM) niche to support leukemia cells by modulating the stromal microenvironment. The aim is to assess Activin-A as a biomarker in acute myeloid leukemia (AML). METHODS: The level of Activin-A and CXCL-12 protein concentration levels in the plasma of bone marrow aspirate samples of eighty AML patients at diagnosis, after induction and at relapse were determined by ELISA. RESULTS: We found that Activin-A concentration levels was significantly up regulated in AML cases at diagnosis, and down regulated at complete remission and rise again at relapse (P< 0.001). In contrast; the CXCL-12 gene expression was significantly down regulated in AML cases at diagnosis; relapse, and up regulated after complete remission (P< 0.001). Multivariate analysis showed that high Activin-A levels at diagnosis is significant predictor of induction of remission response OR 1.006 (CI: 1.002–1.010) (P= 0.003); AML relapse OR 1.002 (CI: 1.0–1.004)...

Asian Pacific Journal of Cancer Prevention, 2020

Background: Myelodysplastic syndromes (MDS) are complex clonal hemopoietic progenitor cell disord... more Background: Myelodysplastic syndromes (MDS) are complex clonal hemopoietic progenitor cell disorders that result from the evolution of aberrant clones which lead to leukemia. Disorders of the immune system serve important functions in the pathophysiology and progression of this disorder. This study aimed to assess the bone marrow natural killer cells percentage as well as soluble TNF-α and sIL-32 concentration levels in MDS patients. Methods: Bone marrow samples were obtained from 34 MDS; 12 MDS-AML and 10 controls. The percentage of total NK cells and mature NK cells were determined by flowcytometry. Bone Marrow soluble TNF-α and sIL-32 concentration levels were measured by ELISA. Results: The percentage of total NK and mature NK cells were significantly lower in MDS patients as compared to controls (P<0.001). The NK cells percentages were significantly related to MDS severity scores being lowest in high score followed by intermediate score and then low score (P<0.001). Moreover; the bone marrow sTNF-α and sIL-32 levels were higher in AML-MDS group; followed by MDS group then the control group and the differences are statistically significant (P<0.001 for both). Conclusion: The reduction in NK cells might have a role in AML evolution on the top of MDS. Likewise, the bone marrow sTNF-α; and sIL32 might have a role in MDS cytopenia.

Cancer Biomarkers, 2021

BACKGROUND: Recent reports indicated the importance of chemotractants CXCL-13 in solid tumors and... more BACKGROUND: Recent reports indicated the importance of chemotractants CXCL-13 in solid tumors and lymphoid malignancies. However, the prognostic value of the mentioned cytokines as biomarkers in chronic lymphocytic leukemia patient’s remains to be identified. Therefore; this study was designed in order to address the relation between CXCL-13 concentrations levels and markers of severity in CLL patients. METHODS: Our study included 150 CLL patients and 20 controls. Serum CXCL-13 was determined by ELISA for CLL patients at diagnosis as well as controls. RESULTS: The serum CXCL-13 levels were significantly higher in CLL patients as compared to controls. The high CXCL-13 concentration levels was significantly associated with high number of smudge cells; high LDH; high grade of Rai stage, short time to first treatment (TTT). Cox regression analysis was conducted for prediction of TTT, using age, gender, WBCs, smudge cells, CXCL-13, LDH, ZAP70, CD38, β2-microglobulin, Rai staging as covar...

Asian Pacific Journal of Cancer Prevention, 2020

Background: Acute myeloid leukemia (AML) escape from immunosurveillance by immunosuppression. CD2... more Background: Acute myeloid leukemia (AML) escape from immunosurveillance by immunosuppression. CD200 and CD56 expression represented an independent prognostic factor in many hematological malignancies but its importance in AML patients remains to be identified. Methods: CD200 and CD56 expression were assessed in the bone marrow blasts for Fifty-two (52) newly diagnosed AML by flowcytometry before start of therapy. Results: CD200 + expression was reported in 28.8% of patients while 17.3% of patients showed CD56 + expression. M4 FAB revealed high frequency of both CD200 + and CD56 + expression. The overall survival of CD200 + patients was 19.2% compared to 35.3% in CD200-(P= 0.049). On the other hand, CD56 + patients had the lowest complete remission rate (22.2% vs. 53.4%). In addition, CD56 + population had significant bad influence on overall survival than those of CD56population (11.1 % vs. 35.5 %, P= 0.047). Conclusions: CD200 and CD56 positive expression by myeloblasts at diagnosis denote poor prognostic indicator and correlated with poor cytogenetic findings. CD200 could be used as therapeutic target in AML.

Cancer Biomarkers, 2020

BACKGROUND : This study aimed to assess the significance of combined expression of interleukin-2 ... more BACKGROUND : This study aimed to assess the significance of combined expression of interleukin-2 receptor (CD25) and the interleukin-3 receptor (CD123) in acute myeloid leukemia (AML) patients. METHODS : The expression of CD25 and CD123 on blast cells in bone marrow samples were identified by flowcytometry in 94 patients (⩽ 60 years old) with de novo acute myeloid leukemia (AML) treated at the Mansoura University Oncology Center (MUOC). RESULTS : Of the 94 samples at diagnosis there were 17 (18.1%) CD25+/CD123+ (double positive) cases; 25 (26.6%) CD25+/CD123- (single positive); 32 (34.0%) CD25-/CD123+ (single positive) cases; 20 (21.3%). CD25-/CD123- (double negative). Most of the AML patients have double CD25+/CD123+ were significantly associated with poor and intermediate risk as compared to those associated with those in the good risk group (P= 0.005). The lowest induction of remission was recorded in AML patients have double CD25+/CD123+ expression as compared to the remaining A...

Asian Pacific Journal of Cancer Prevention, 2021

Pui 2013). Bone marrow (BM) relapse most common than extramedullary infiltration such as central ... more Pui 2013). Bone marrow (BM) relapse most common than extramedullary infiltration such as central nervous system (CNS); with a generally worse survival (Malempati et al., 2007). Several studies have demonstrated the role

Asian Pacific Journal of Cancer Prevention, 2020

About eleven human TLRs have been identified up till now and each of them participates in a speci... more About eleven human TLRs have been identified up till now and each of them participates in a specific intracellular signaling pathway. TLRs 1, 2, 4, 5 and 6 are typical for bacterial products, TLRs 3, 7 and 8 are characteristic for viral infection and TLR9 is associated

Asian Pacific Journal of Cancer Prevention, 2020

The clinical course of Myelodysplastic Syndromes (MDS) are very heterogeneous, with a wide divers... more The clinical course of Myelodysplastic Syndromes (MDS) are very heterogeneous, with a wide diversity in patient's outcome. Whilst near one third of MDS progress to AML; the other two thirds transformed from low risk into high risk MDS (Garcia-Manero 2015

Clinical Lymphoma Myeloma and Leukemia, 2020

Hematology, 2006

Background: Disorders of the cell cycle regulatory machinery play a key role in the pathogenesis ... more Background: Disorders of the cell cycle regulatory machinery play a key role in the pathogenesis of cancer. Over expression of cyclin D1 protein has been reported in several solid tumors and certain lymphoid malignancies, but little is known about the involvement of cyclin D1 in ...

Pediatric Hematology and Oncology

This study aimed to address the clinical impact of recombinationactivating gene (RAG1) expression... more This study aimed to address the clinical impact of recombinationactivating gene (RAG1) expression and tumor suppressor IKZF1 gene deletions in Philadelphia negative B-cell precursor acute lymphoblastic leukemia (B-ALL) patients. Fifty newly diagnosed pediatric Philadelphia negative BALL patients were included in this study. Using Bone Marrow samples, RAG1 expression was assessed by real time PCR and IKZF1 deletions were determined by multiplex realtime quantitative PCR. The expression of RAG1 was significantly higher in BALL patients as compared to the controls (p < .001). The BALL patients with RAG1 high expression (median) had lower response to induction of remission, shorter DFS, shorter overall survival, higher blast cells, and white cell counts in the peripheral blood as compared to those with low RAG1 expression levels (p < .01 for all). Likewise, there was significant association between IKZF1 deletion and high RAG1 expression. Based on our findings RAG1 high expression and IKZF1 deletions were associated with adverse prognosis in Philadelphia negative BALL. RAG1 could be used as therapeutic target in the treatment of BALL .

Pediatric Hematology and Oncology

This study aimed to address the clinical impact of recombinationactivating gene (RAG1) expression... more This study aimed to address the clinical impact of recombinationactivating gene (RAG1) expression and tumor suppressor IKZF1 gene deletions in Philadelphia negative B-cell precursor acute lymphoblastic leukemia (B-ALL) patients. Fifty newly diagnosed pediatric Philadelphia negative BALL patients were included in this study. Using Bone Marrow samples, RAG1 expression was assessed by real time PCR and IKZF1 deletions were determined by multiplex realtime quantitative PCR. The expression of RAG1 was significantly higher in BALL patients as compared to the controls (p < .001). The BALL patients with RAG1 high expression (median) had lower response to induction of remission, shorter DFS, shorter overall survival, higher blast cells, and white cell counts in the peripheral blood as compared to those with low RAG1 expression levels (p < .01 for all). Likewise, there was significant association between IKZF1 deletion and high RAG1 expression. Based on our findings RAG1 high expression and IKZF1 deletions were associated with adverse prognosis in Philadelphia negative BALL. RAG1 could be used as therapeutic target in the treatment of BALL .

Asian Pacific journal of cancer prevention : APJCP, 2021

BACKGROUND Bone marrow myelofibrosis (BMF) that develop on top of Polycythaemia vera (PV) and ess... more BACKGROUND Bone marrow myelofibrosis (BMF) that develop on top of Polycythaemia vera (PV) and essential thrombocythemia leads to shortening of the patient's overall survival. This study aimed to address the impact of miR-146a rs2431697 polymorphism on inflammatory biomarkers and genes expression and the hazards of myelofibrosis progression. PATIENTS AND METHODS The study included 88 myeloproliferative neoplasm (40 PV; 27 ET; 21 MF) and 90 healthy controls. For all investigated subjects miR-146a rs2431697 genotypes were identified by sequencing and the expression of miR-146a; IL-1β; NF-κB; a NOD-like receptor family, pyrin domain containing 3 (NLRP3) (NLRP3) genes were estimated by real time PCR. RESULTS miR146a genotypes revealed that there was significant association between TT and TC genotypes with MF. The degree of miR146a expression was significantly reduced in MF as compared to both PV and ET. In contrast; the levels of IL-1β; NF-κB; NLRP3 genes expression were significantl...

Journal of Pediatric Hematology/Oncology, 2022

INTRODUCTION Pediatric immune thrombocytopenia (ITP) is a potentially life threating autoimmune d... more INTRODUCTION Pediatric immune thrombocytopenia (ITP) is a potentially life threating autoimmune disorder with different responses to therapy and different bleeding phenotypes in critical organs. The molecular basis for the variable response has not yet been fully elucidated. This study was designed to address the predictive value of regulatory B-cell (Breg) count and interleukin-10 (IL-10) serum levels for acute ITP patients who progress to chronic phase. The present study included 80 children with acute ITP )38 males and 42 females (with median age of 8 years and 40 matched healthy controls. Assessment of Breg (CD19+CD24hiCD38hi) was carried out by a multicolor flowcytometry, however, IL-10 serum levels were evaluated by enzyme-linked immunosorbent assay. A significant reduction of Breg percentage and a significant increase in serum IL-10 levels were identified in children with acute ITP as compared with controls (P<0.001 for both). Fourteen ITP patients passed to chronic phase, while 66 patients achieved remission within 6 months. The absolute Breg was significantly lower, while IL-10 was significantly higher in patients with acute ITP who progressed to chronic phase in comparison with acute ITP patients who achieved complete remission. Cox proportional hazards for ITP chronicity revealed that IL-10 OR was 2.46 (confidence interval: 1.42-4.27; P=0.001) and absolute Breg OR was 0.147 (confidence interval: 0.128-0.624; P=0.005) in the peripheral blood. Therefore, they could predict chronicity in ITP cases. CONCLUSION Reduced Breg count and elevated IL-10 levels in patients with acute ITP at diagnosis can predict chronicity.

Asian Pacific Journal of Cancer Prevention, 2021

Background: Recent reports suggested relation between Interferon Gamma (IFN-γ) gene polymorphism ... more Background: Recent reports suggested relation between Interferon Gamma (IFN-γ) gene polymorphism and the risk of development of HCC on top of hepatic cirrhosis. The aim of this study was to address the predictive value of Interferon Gamma gene receptor (IFN-γR) polymorphisms for the occurrence of hepatocellular carcinoma on top of liver cirrhosis. Patients and Methods: This is a case control study performed on patients selected from the outpatient hepatology clinic, specialized medical hospital, Mansoura University, Egypt, from August 2017 to February 2019. The included patients were categorized into two groups; 60 patients with HCC on top of cirrhosis and 20 patients with hepatic cirrhosis. For all patients IFN-γR polymorphism was identified by RFLP. Results: Our study showed that HCC patients had male predominance. Additionally, diabetes mellitus (DM) was found in 28.3% of total HCC patients. Half of HCC patients in this study were from rural areas (50%). The frequency of AA at position-611 in the IFN-γR (-611 IFN-γR) was significantly higher in the HCC group as compared to cirrhotic group (P=0.021). Moreover; the frequency of CC and CT genotypes of IFN-γR-56 was not significantly different in HCC group as compared to control group (P>0.05). The IFN-γR (-611 IFN-γ) AA genotype significantly increased risk of HCC (OR= 0.78, 95% CI= 0.10-6.39; P= 0.042). Conclusion: The analysis of IFN-γR-611 single nucleotide gene polymorphism could be a valuable marker for predicting subgroup of cirrhotic patients with high risk of developing HCC. Cirrhotic patients have AA genotype of IFN-γR-611 recommended to be under close follow up.

Clinical Lymphoma Myeloma and Leukemia, 2021

This study aimed to address the prognostic relevance of CD34+/CD38-/TIM3+ leukemic stem cell (LSC... more This study aimed to address the prognostic relevance of CD34+/CD38-/TIM3+ leukemic stem cell (LSC) frequency in patients with acute myeloid leukemia (AML) and its impact on patient outcome. We analyzed the expression of LSC markers (CD34+/CD38-/TIM3+) using flow cytometry in bone marrow samples of 53 AML cases before and after induction chemotherapy. The LSC frequency at diagnosis was significantly higher compared with that postinduction (P < .001). Patients were categorized into high LSC expressers (≥ median) and low expressers (< median). Patients with AML with high number of LSCs at diagnosis had significantly lower induction of remission response (P = .0104), shorter disease-free survival, and shorter overall survival (P < .001 for both) compared with those with lower LSC count. Cox regression analysis revealed that LSC frequency at diagnosis is an independent prognostic factor in AML. Assessment of LSCs (CD34+/CD38-/TIM3+) at diagnosis is recommended for refining of AML risk stratification.

Clinical Lymphoma Myeloma and Leukemia, 2021

Asian Pacific Journal of Cancer Prevention : APJCP, 2021

Background: Transducin-like enhancer of split 1 (TLE1( is a member of the TLE family of transcrip... more Background: Transducin-like enhancer of split 1 (TLE1( is a member of the TLE family of transcriptional co-repressors that control the transcription of a wide range of genes. The aim of this study was to evaluate the prognostic role of TLE1 gene expression in patients with T-cell acute lymphoblastic leukemia (T-ALL). Method: This study was conducted on 97 newly diagnosed T-ALL patients admitted to the Mansoura University oncology center (59 males and 38 females) with median age (33 years) in addition to 102 apparently healthy individuals served as a control group. TLE1 gene expression was measured in both patients and control groups by real time – PCR. The calculation of relative gene expression was done using the ΔΔCt method. Results: TEL1 gene expression was significantly down regulated in T-ALL cases (median 2.83) as compared to controls (median 84.65) (p < 0.001). The low TEL1 gene expression was significantly associated with CNS infiltration, non-remission and higher relapse...

The consultants Meeting on Cancer Medicine, Radiology & Treatment 2020 is planned to be conducted... more The consultants Meeting on Cancer Medicine, Radiology & Treatment 2020 is planned to be conducted within the month of Gregorian calendar month 29-30, 2020 at Athens, Greece. This Conference is CME & CPD authorized. Cancer meeting summit 2020 are going to be organized around the theme “Revolutions in Cancer Prevention and Diagnosis”.

Cancer Biomarkers, 2021

BACKGROUND: Acute myeloid leukemia changes the bone marrow (BM) niche to support leukemia cells b... more BACKGROUND: Acute myeloid leukemia changes the bone marrow (BM) niche to support leukemia cells by modulating the stromal microenvironment. The aim is to assess Activin-A as a biomarker in acute myeloid leukemia (AML). METHODS: The level of Activin-A and CXCL-12 protein concentration levels in the plasma of bone marrow aspirate samples of eighty AML patients at diagnosis, after induction and at relapse were determined by ELISA. RESULTS: We found that Activin-A concentration levels was significantly up regulated in AML cases at diagnosis, and down regulated at complete remission and rise again at relapse (P< 0.001). In contrast; the CXCL-12 gene expression was significantly down regulated in AML cases at diagnosis; relapse, and up regulated after complete remission (P< 0.001). Multivariate analysis showed that high Activin-A levels at diagnosis is significant predictor of induction of remission response OR 1.006 (CI: 1.002–1.010) (P= 0.003); AML relapse OR 1.002 (CI: 1.0–1.004)...

Asian Pacific Journal of Cancer Prevention, 2020

Background: Myelodysplastic syndromes (MDS) are complex clonal hemopoietic progenitor cell disord... more Background: Myelodysplastic syndromes (MDS) are complex clonal hemopoietic progenitor cell disorders that result from the evolution of aberrant clones which lead to leukemia. Disorders of the immune system serve important functions in the pathophysiology and progression of this disorder. This study aimed to assess the bone marrow natural killer cells percentage as well as soluble TNF-α and sIL-32 concentration levels in MDS patients. Methods: Bone marrow samples were obtained from 34 MDS; 12 MDS-AML and 10 controls. The percentage of total NK cells and mature NK cells were determined by flowcytometry. Bone Marrow soluble TNF-α and sIL-32 concentration levels were measured by ELISA. Results: The percentage of total NK and mature NK cells were significantly lower in MDS patients as compared to controls (P<0.001). The NK cells percentages were significantly related to MDS severity scores being lowest in high score followed by intermediate score and then low score (P<0.001). Moreover; the bone marrow sTNF-α and sIL-32 levels were higher in AML-MDS group; followed by MDS group then the control group and the differences are statistically significant (P<0.001 for both). Conclusion: The reduction in NK cells might have a role in AML evolution on the top of MDS. Likewise, the bone marrow sTNF-α; and sIL32 might have a role in MDS cytopenia.

Cancer Biomarkers, 2021

BACKGROUND: Recent reports indicated the importance of chemotractants CXCL-13 in solid tumors and... more BACKGROUND: Recent reports indicated the importance of chemotractants CXCL-13 in solid tumors and lymphoid malignancies. However, the prognostic value of the mentioned cytokines as biomarkers in chronic lymphocytic leukemia patient’s remains to be identified. Therefore; this study was designed in order to address the relation between CXCL-13 concentrations levels and markers of severity in CLL patients. METHODS: Our study included 150 CLL patients and 20 controls. Serum CXCL-13 was determined by ELISA for CLL patients at diagnosis as well as controls. RESULTS: The serum CXCL-13 levels were significantly higher in CLL patients as compared to controls. The high CXCL-13 concentration levels was significantly associated with high number of smudge cells; high LDH; high grade of Rai stage, short time to first treatment (TTT). Cox regression analysis was conducted for prediction of TTT, using age, gender, WBCs, smudge cells, CXCL-13, LDH, ZAP70, CD38, β2-microglobulin, Rai staging as covar...

Asian Pacific Journal of Cancer Prevention, 2020

Background: Acute myeloid leukemia (AML) escape from immunosurveillance by immunosuppression. CD2... more Background: Acute myeloid leukemia (AML) escape from immunosurveillance by immunosuppression. CD200 and CD56 expression represented an independent prognostic factor in many hematological malignancies but its importance in AML patients remains to be identified. Methods: CD200 and CD56 expression were assessed in the bone marrow blasts for Fifty-two (52) newly diagnosed AML by flowcytometry before start of therapy. Results: CD200 + expression was reported in 28.8% of patients while 17.3% of patients showed CD56 + expression. M4 FAB revealed high frequency of both CD200 + and CD56 + expression. The overall survival of CD200 + patients was 19.2% compared to 35.3% in CD200-(P= 0.049). On the other hand, CD56 + patients had the lowest complete remission rate (22.2% vs. 53.4%). In addition, CD56 + population had significant bad influence on overall survival than those of CD56population (11.1 % vs. 35.5 %, P= 0.047). Conclusions: CD200 and CD56 positive expression by myeloblasts at diagnosis denote poor prognostic indicator and correlated with poor cytogenetic findings. CD200 could be used as therapeutic target in AML.

Cancer Biomarkers, 2020

BACKGROUND : This study aimed to assess the significance of combined expression of interleukin-2 ... more BACKGROUND : This study aimed to assess the significance of combined expression of interleukin-2 receptor (CD25) and the interleukin-3 receptor (CD123) in acute myeloid leukemia (AML) patients. METHODS : The expression of CD25 and CD123 on blast cells in bone marrow samples were identified by flowcytometry in 94 patients (⩽ 60 years old) with de novo acute myeloid leukemia (AML) treated at the Mansoura University Oncology Center (MUOC). RESULTS : Of the 94 samples at diagnosis there were 17 (18.1%) CD25+/CD123+ (double positive) cases; 25 (26.6%) CD25+/CD123- (single positive); 32 (34.0%) CD25-/CD123+ (single positive) cases; 20 (21.3%). CD25-/CD123- (double negative). Most of the AML patients have double CD25+/CD123+ were significantly associated with poor and intermediate risk as compared to those associated with those in the good risk group (P= 0.005). The lowest induction of remission was recorded in AML patients have double CD25+/CD123+ expression as compared to the remaining A...

Asian Pacific Journal of Cancer Prevention, 2021

Pui 2013). Bone marrow (BM) relapse most common than extramedullary infiltration such as central ... more Pui 2013). Bone marrow (BM) relapse most common than extramedullary infiltration such as central nervous system (CNS); with a generally worse survival (Malempati et al., 2007). Several studies have demonstrated the role

Asian Pacific Journal of Cancer Prevention, 2020

About eleven human TLRs have been identified up till now and each of them participates in a speci... more About eleven human TLRs have been identified up till now and each of them participates in a specific intracellular signaling pathway. TLRs 1, 2, 4, 5 and 6 are typical for bacterial products, TLRs 3, 7 and 8 are characteristic for viral infection and TLR9 is associated

Asian Pacific Journal of Cancer Prevention, 2020

The clinical course of Myelodysplastic Syndromes (MDS) are very heterogeneous, with a wide divers... more The clinical course of Myelodysplastic Syndromes (MDS) are very heterogeneous, with a wide diversity in patient's outcome. Whilst near one third of MDS progress to AML; the other two thirds transformed from low risk into high risk MDS (Garcia-Manero 2015

Clinical Lymphoma Myeloma and Leukemia, 2020