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Research paper thumbnail of Nanoliposomal formulation of Agrostemma githago aqueous extract shows enhanced cytotoxic effect on gastric cancer cell line

Objective(s): The objective of this study was to determine the cytotoxic effects of nanoliposomal... more Objective(s): The objective of this study was to determine the cytotoxic effects of nanoliposomal form of lyophilized aqueous extract of Agrostemma githago (A. githago) seeds on gastric cancer cell line (AGS) using cell viability tests. Materials and Methods: Lyophilized aqueous extract of A. githago seeds was prepared. Liposomes were also prepared by thin-film hydration method and their stability and size were characterized by SEM. The size and zeta potential were determined by Malvern Zetasizer. Cytotoxic effects of nanoliposomes on gastric cancer cell line was determined using MTT, Neutral Red and Frame methods. Results: The size of liposomes was around 171.5 nm with proper dispersion (PDI=0.268). The morphology of the liposomes was suitable according to SEM images. The IC 50 values indicated that the nanoliposomal form of extract was 3-4 times more active than extract alone. Average IC 50 values for extract and liposomal form of extract were 13.02 ± 0.95 and 4.43 ± 1.49 ug/ml, respectively. Conclusion: This study showed that liposomal form of aqueous extract of A. githago seeds exerts cytotoxic effect at significantly lower concentrations than the extract itself.

Research paper thumbnail of Inhibition of EGFR expression with chitosan/alginate nanoparticles encapsulating antisense oligonucleotides in T47D cell line using RT-PCR and …

Carbohydrate …, 2010

... immunocytochemistry. Taraneh Gazori a , Ismaeil Haririan a , b , Shamileh Fouladdel c , Alire... more ... immunocytochemistry. Taraneh Gazori a , Ismaeil Haririan a , b , Shamileh Fouladdel c , Alireza Namazi c , Alireza Nomani a , d and Ebrahim Azizi c , e , Corresponding Author Contact Information , E-mail The Corresponding Author. ...

Research paper thumbnail of Novel pectinase from Piriformospora indica, optimization of growth parameters and enzyme production in submerged culture condition

Turkish Journal of Biochemistry

Background:Pectinases are one of the upcoming enzymes in food processing industries and they hydr... more Background:Pectinases are one of the upcoming enzymes in food processing industries and they hydrolyze pectin substances.Objectives:This study was done to examine the production of pectinase byPiriformospora indicain submerged fermentation (SmF) along with growth parameters.Materials and methods:The fungusP. indicawas cultured on Kafer medium supplemented with pectin for 0–12 days and fungus growth, number of spores, total protein content, and pectinase activity were investigated.Results:Firstly, pectinase secretion byP. indicawas confirmed by cup-plate assay. The maximum dry cell weight (10.21 g/L), growth yield (0.65 g/g), specific growth rate (0.56 day−1) and pectinase activity (10.47 U/mL) on pectin containing medium (P+) were achieved after 6 day of culture. In the case of pectin free medium (P−) all the parameters were less than P+medium. The pectinase production byP. indicaon P+was 2.7 times higher than P−. The optimum pH and temperature for maximum polygalacturonase activity...

Research paper thumbnail of Silica Nanostructures, a Heterogeneous Surface for Dendrimer Functionalization

Research paper thumbnail of Dithiocarbamate to modify magnetic graphene oxide nanocomposite (Fe3O4-GO): A new strategy for covalent enzyme (lipase) immobilization to fabrication a new nanobiocatalyst for enzymatic hydrolysis of PNPD

International journal of biological macromolecules, 2017

Immobilization of lipase was successfully achieved on the surface of magnetically separable Fe3O4... more Immobilization of lipase was successfully achieved on the surface of magnetically separable Fe3O4/graphene oxide (GO) via a post-modification. This post modification was achieved in alternation to glutaraldehyde post-modification. The activity of immobilized lipase had not a significant loss in the activity while on the other hand, it is simply extractable (by keeping its major activity) from reaction crude by a magnet. Each step of immobilization was carefully monitored by characterization and all were successfully proved. SEM, TEM, XRD, EDX, and FTIR were used to characterize the support and immobilization process.

Research paper thumbnail of Covalently bonded pancreatic lipase onto the dithiocarbamate/chitosan-based magnetite: Stepwise fabrication of Fe3O4@CS/NHCS-Lip as a novel and promising nanobiocatalyst

International journal of biological macromolecules, 2017

In the present paper, porcine pancreas lipase (PPL) was immobilized to a new version of magnetite... more In the present paper, porcine pancreas lipase (PPL) was immobilized to a new version of magnetite via a novel stepwise dithiocarbamate/chitosan-based method in alternation to glutaraldehyde. Magnetic chitosan nanocomposite was post-modified to produce dithiocarbamate moieties on the surface through amine functions. Then, immobilization of lipase was successfully achieved on the surface of magnetically separable Fe3O4@CS/NHCS2H via a post-modification. Each step of immobilization was carefully monitored by characterization and all were successfully proved. Comparison of immobilized enzyme with free enzyme showed that the method of immobilization is efficient.

Research paper thumbnail of Chitosan prevents oxidative stress-induced amyloid β formation and cytotoxicity in NT2 neurons: involvement of transcription factors Nrf2 and NF-κB

Molecular and Cellular Biochemistry, 2010

Increased oxidative stress is a widely accepted factor in the development and progression of Alzh... more Increased oxidative stress is a widely accepted factor in the development and progression of Alzheimer's disease. Here, we introduce chitosan, an antioxidant oligosaccharide, as a protective agent against H(2)O(2)/FeSO(4)-induced cell death in the NT2 neural cell line. Chitosan not only protects the neurons against cell death, as measured by MTT and caspase-3 activity, but also decreases amyloid beta formation. NT2 neurons can be used to elucidate the relationship between oxidative stress and Abeta formation. We induced Abeta formation through oxidative stress in NT2 neurons and studied the effect of chitosan. We demonstrate that chitosan can be neuroprotective by suppressing Abeta formation. We further show that chitosan exerts its protective effect by up-regulation of HO-1, gamma-GCS, Hsp-70, and Nrf2, while it inhibits activation of caspase-3 and NF-kappaB. Chitosan or chitosan derivatives have potential value as neuroprotective agents, particularly with regard to oxidative stress.

Research paper thumbnail of Cell cycle alterations, apoptosis induction and caspase activation in breast cancer T47D cells treated with Baneh extract or doxorubicin

Fuel and Energy Abstracts, 2011

Research paper thumbnail of Comparative cellular and molecular analysis of cytotoxicity and apoptosis induction by doxorubicin and Baneh in human breast cancer T47D cells

Cytotechnology

It is now widely accepted that dietary phytochemicals inhibit cancer progression and enhance the ... more It is now widely accepted that dietary phytochemicals inhibit cancer progression and enhance the effects of conventional chemotherapy. In this report, we comparatively studied the cellular and molecular aspects of apoptosis induction by the methanolic extract of Baneh fruit skin in comparison to Doxorubicin (Dox), a well-known anticancer drug, in human breast cancer T47D cells. The MTT assay was used to determine the antiproliferative effects. The flow cytometric and microscopic analyses were done to evaluate the apoptosis induction. Furthermore, western blot analyses have been done to study the role of key molecular players of apoptosis including caspase 3 and PARP. The Baneh extract showed strong antiproliferative activity against T47D cells in a dose-and time-dependent manner that was comparable to and even stronger than Dox in certain concentrations. Analysis of Baneh-treated cells by flow cytometry and fluorescence microscopy indicated strong apoptosis induction and nuclear morphological alterations similar to or greater than Dox. Finally, molecular analysis of apoptosis by western blotting proved activation of caspase 3 followed by poly ADP ribose polymerase (PARP) cleavage more efficiently in Baneh than in Dox treated cancer cells. These findings indicate that Baneh extract contains phytochemicals which act as inhibitor of cell proliferation and inducer of apoptosis in human breast cancer T47D cells that makes it a potentially good candidate for new anticancer drug development.

Research paper thumbnail of Chitosan prevents oxidative stress-induced amyloid β formation and cytotoxicity in NT2 neurons: involvement of transcription factors Nrf2 and NF-κB

Molecular and Cellular Biochemistry, 2010

Increased oxidative stress is a widely accepted factor in the development and progression of Alzh... more Increased oxidative stress is a widely accepted factor in the development and progression of Alzheimer's disease. Here, we introduce chitosan, an antioxidant oligosaccharide, as a protective agent against H(2)O(2)/FeSO(4)-induced cell death in the NT2 neural cell line. Chitosan not only protects the neurons against cell death, as measured by MTT and caspase-3 activity, but also decreases amyloid beta formation. NT2 neurons can be used to elucidate the relationship between oxidative stress and Abeta formation. We induced Abeta formation through oxidative stress in NT2 neurons and studied the effect of chitosan. We demonstrate that chitosan can be neuroprotective by suppressing Abeta formation. We further show that chitosan exerts its protective effect by up-regulation of HO-1, gamma-GCS, Hsp-70, and Nrf2, while it inhibits activation of caspase-3 and NF-kappaB. Chitosan or chitosan derivatives have potential value as neuroprotective agents, particularly with regard to oxidative stress.

Research paper thumbnail of Nanoliposomal formulation of Agrostemma githago aqueous extract shows enhanced cytotoxic effect on gastric cancer cell line

Objective(s): The objective of this study was to determine the cytotoxic effects of nanoliposomal... more Objective(s): The objective of this study was to determine the cytotoxic effects of nanoliposomal form of lyophilized aqueous extract of Agrostemma githago (A. githago) seeds on gastric cancer cell line (AGS) using cell viability tests. Materials and Methods: Lyophilized aqueous extract of A. githago seeds was prepared. Liposomes were also prepared by thin-film hydration method and their stability and size were characterized by SEM. The size and zeta potential were determined by Malvern Zetasizer. Cytotoxic effects of nanoliposomes on gastric cancer cell line was determined using MTT, Neutral Red and Frame methods. Results: The size of liposomes was around 171.5 nm with proper dispersion (PDI=0.268). The morphology of the liposomes was suitable according to SEM images. The IC 50 values indicated that the nanoliposomal form of extract was 3-4 times more active than extract alone. Average IC 50 values for extract and liposomal form of extract were 13.02 ± 0.95 and 4.43 ± 1.49 ug/ml, respectively. Conclusion: This study showed that liposomal form of aqueous extract of A. githago seeds exerts cytotoxic effect at significantly lower concentrations than the extract itself.

Research paper thumbnail of Inhibition of EGFR expression with chitosan/alginate nanoparticles encapsulating antisense oligonucleotides in T47D cell line using RT-PCR and …

Carbohydrate …, 2010

... immunocytochemistry. Taraneh Gazori a , Ismaeil Haririan a , b , Shamileh Fouladdel c , Alire... more ... immunocytochemistry. Taraneh Gazori a , Ismaeil Haririan a , b , Shamileh Fouladdel c , Alireza Namazi c , Alireza Nomani a , d and Ebrahim Azizi c , e , Corresponding Author Contact Information , E-mail The Corresponding Author. ...

Research paper thumbnail of Novel pectinase from Piriformospora indica, optimization of growth parameters and enzyme production in submerged culture condition

Turkish Journal of Biochemistry

Background:Pectinases are one of the upcoming enzymes in food processing industries and they hydr... more Background:Pectinases are one of the upcoming enzymes in food processing industries and they hydrolyze pectin substances.Objectives:This study was done to examine the production of pectinase byPiriformospora indicain submerged fermentation (SmF) along with growth parameters.Materials and methods:The fungusP. indicawas cultured on Kafer medium supplemented with pectin for 0–12 days and fungus growth, number of spores, total protein content, and pectinase activity were investigated.Results:Firstly, pectinase secretion byP. indicawas confirmed by cup-plate assay. The maximum dry cell weight (10.21 g/L), growth yield (0.65 g/g), specific growth rate (0.56 day−1) and pectinase activity (10.47 U/mL) on pectin containing medium (P+) were achieved after 6 day of culture. In the case of pectin free medium (P−) all the parameters were less than P+medium. The pectinase production byP. indicaon P+was 2.7 times higher than P−. The optimum pH and temperature for maximum polygalacturonase activity...

Research paper thumbnail of Silica Nanostructures, a Heterogeneous Surface for Dendrimer Functionalization

Research paper thumbnail of Dithiocarbamate to modify magnetic graphene oxide nanocomposite (Fe3O4-GO): A new strategy for covalent enzyme (lipase) immobilization to fabrication a new nanobiocatalyst for enzymatic hydrolysis of PNPD

International journal of biological macromolecules, 2017

Immobilization of lipase was successfully achieved on the surface of magnetically separable Fe3O4... more Immobilization of lipase was successfully achieved on the surface of magnetically separable Fe3O4/graphene oxide (GO) via a post-modification. This post modification was achieved in alternation to glutaraldehyde post-modification. The activity of immobilized lipase had not a significant loss in the activity while on the other hand, it is simply extractable (by keeping its major activity) from reaction crude by a magnet. Each step of immobilization was carefully monitored by characterization and all were successfully proved. SEM, TEM, XRD, EDX, and FTIR were used to characterize the support and immobilization process.

Research paper thumbnail of Covalently bonded pancreatic lipase onto the dithiocarbamate/chitosan-based magnetite: Stepwise fabrication of Fe3O4@CS/NHCS-Lip as a novel and promising nanobiocatalyst

International journal of biological macromolecules, 2017

In the present paper, porcine pancreas lipase (PPL) was immobilized to a new version of magnetite... more In the present paper, porcine pancreas lipase (PPL) was immobilized to a new version of magnetite via a novel stepwise dithiocarbamate/chitosan-based method in alternation to glutaraldehyde. Magnetic chitosan nanocomposite was post-modified to produce dithiocarbamate moieties on the surface through amine functions. Then, immobilization of lipase was successfully achieved on the surface of magnetically separable Fe3O4@CS/NHCS2H via a post-modification. Each step of immobilization was carefully monitored by characterization and all were successfully proved. Comparison of immobilized enzyme with free enzyme showed that the method of immobilization is efficient.

Research paper thumbnail of Chitosan prevents oxidative stress-induced amyloid β formation and cytotoxicity in NT2 neurons: involvement of transcription factors Nrf2 and NF-κB

Molecular and Cellular Biochemistry, 2010

Increased oxidative stress is a widely accepted factor in the development and progression of Alzh... more Increased oxidative stress is a widely accepted factor in the development and progression of Alzheimer's disease. Here, we introduce chitosan, an antioxidant oligosaccharide, as a protective agent against H(2)O(2)/FeSO(4)-induced cell death in the NT2 neural cell line. Chitosan not only protects the neurons against cell death, as measured by MTT and caspase-3 activity, but also decreases amyloid beta formation. NT2 neurons can be used to elucidate the relationship between oxidative stress and Abeta formation. We induced Abeta formation through oxidative stress in NT2 neurons and studied the effect of chitosan. We demonstrate that chitosan can be neuroprotective by suppressing Abeta formation. We further show that chitosan exerts its protective effect by up-regulation of HO-1, gamma-GCS, Hsp-70, and Nrf2, while it inhibits activation of caspase-3 and NF-kappaB. Chitosan or chitosan derivatives have potential value as neuroprotective agents, particularly with regard to oxidative stress.

Research paper thumbnail of Cell cycle alterations, apoptosis induction and caspase activation in breast cancer T47D cells treated with Baneh extract or doxorubicin

Fuel and Energy Abstracts, 2011

Research paper thumbnail of Comparative cellular and molecular analysis of cytotoxicity and apoptosis induction by doxorubicin and Baneh in human breast cancer T47D cells

Cytotechnology

It is now widely accepted that dietary phytochemicals inhibit cancer progression and enhance the ... more It is now widely accepted that dietary phytochemicals inhibit cancer progression and enhance the effects of conventional chemotherapy. In this report, we comparatively studied the cellular and molecular aspects of apoptosis induction by the methanolic extract of Baneh fruit skin in comparison to Doxorubicin (Dox), a well-known anticancer drug, in human breast cancer T47D cells. The MTT assay was used to determine the antiproliferative effects. The flow cytometric and microscopic analyses were done to evaluate the apoptosis induction. Furthermore, western blot analyses have been done to study the role of key molecular players of apoptosis including caspase 3 and PARP. The Baneh extract showed strong antiproliferative activity against T47D cells in a dose-and time-dependent manner that was comparable to and even stronger than Dox in certain concentrations. Analysis of Baneh-treated cells by flow cytometry and fluorescence microscopy indicated strong apoptosis induction and nuclear morphological alterations similar to or greater than Dox. Finally, molecular analysis of apoptosis by western blotting proved activation of caspase 3 followed by poly ADP ribose polymerase (PARP) cleavage more efficiently in Baneh than in Dox treated cancer cells. These findings indicate that Baneh extract contains phytochemicals which act as inhibitor of cell proliferation and inducer of apoptosis in human breast cancer T47D cells that makes it a potentially good candidate for new anticancer drug development.

Research paper thumbnail of Chitosan prevents oxidative stress-induced amyloid β formation and cytotoxicity in NT2 neurons: involvement of transcription factors Nrf2 and NF-κB

Molecular and Cellular Biochemistry, 2010

Increased oxidative stress is a widely accepted factor in the development and progression of Alzh... more Increased oxidative stress is a widely accepted factor in the development and progression of Alzheimer's disease. Here, we introduce chitosan, an antioxidant oligosaccharide, as a protective agent against H(2)O(2)/FeSO(4)-induced cell death in the NT2 neural cell line. Chitosan not only protects the neurons against cell death, as measured by MTT and caspase-3 activity, but also decreases amyloid beta formation. NT2 neurons can be used to elucidate the relationship between oxidative stress and Abeta formation. We induced Abeta formation through oxidative stress in NT2 neurons and studied the effect of chitosan. We demonstrate that chitosan can be neuroprotective by suppressing Abeta formation. We further show that chitosan exerts its protective effect by up-regulation of HO-1, gamma-GCS, Hsp-70, and Nrf2, while it inhibits activation of caspase-3 and NF-kappaB. Chitosan or chitosan derivatives have potential value as neuroprotective agents, particularly with regard to oxidative stress.