Francielle Sato | Universidade Estadual de Maringa (original) (raw)

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Papers by Francielle Sato

International Journal of Pharmaceutics, 2015

The pressure ulcer healing is a complex process and difficult to be achieved. Insulin is known to... more The pressure ulcer healing is a complex process and difficult to be achieved. Insulin is known to promote wound healing, and when complexed with cyclodextrin presents improved solubility, stability and biological activity. Complexation of insulin with hydroxypropyl-beta-cyclodextrin (HPβCD) was performed in this work through the coprecipitation method, providing the inclusion complex (HPβCD-I). The spectroscopic techniques used to analyze the complex were H(1) NMR, FT-Raman and FT-IR/ATR. A gel containing the HPβCD-I complex was prepared and a clinical study was conducted in patients with pressure ulcers. The spectroscopic techniques allowed to confirm the complex formation through the inclusion of aromatic amino acids, such as phenylalanine present in the HPβCD cavity. Data obtained from the FT-Raman and FT-IR/ATR techniques, combined with the H(1) NMR results, showed the effectiveness of these techniques in evaluating the inclusion complex of HPβCD with insulin. Clinical studies demonstrated tissue revitalization and a trend (p=0.06) for a significant difference between the healing effect of the control gel and that with HPβCD-I complex. The creation of the gel prepared with insulin and HPβCD-I complex and its use in patients with pressure ulcers appears to be promising in wound healing and its possible use in hospital care.

Journal of Esthetic and Restorative Dentistry, 2014

To evaluate the color match of different composite resins relative to Vitapan Classical shade gui... more To evaluate the color match of different composite resins relative to Vitapan Classical shade guide tab and their respective manufacturers' shade guide tabs as a function of time and storage. Three enamel shade A2 composite resins were used to fabricate 36 disk-shaped polymerized specimens (12 each), allocated into 2 groups of 6 and stored dry (GD) and in artificial saliva (GS). CIELAB coordinates from shade tabs and resin specimens immediately after polymerization (t0), and 24 hours (t1), 7 (t7), 14 (t14) and 21 (t21) days after polymerization were captured using a colorimeter. Color difference (ΔE00 ) between composite specimens and the reference tabs was calculated using the CIEDE2000 formula. The results were analyzed with repeated measures ANOVA, Tukey's HDS post-hoc test, and Student t test (p ≤ 0.05). Color of the three tested composites relative to Vita and their respective tabs significantly changed as a function of time until t14; however, between t14 and t21, no significant differences were found. No differences in color were found relative to storage at t14 and t21. ΔE00 values of specimens at t14 were significantly higher relative to their respective tabs than to Vitapan tab. For all brands color changed up to day 14, when it stabilized, regardless of whether composite specimens had been stored in artificial saliva or simply in a box. Vitapan tab presented a better color match than the manufacturers' tabs. The results found in this study demonstrated that the Vitapan Classical shade guide tab A2 provided a better color match than the respective shade guide tabs A2 supplied by the composite manufacturers. If custom shade tabs are to be made, however, they could be kept in a box and used as shade references from 14 days after being fabricated, when color stabilizes.

Journal of Raman Spectroscopy, 2014

Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, 2014

Photochemistry and Photobiology, 2013

This study evaluated physical, chemical and morphological changes in lungs of mice infected with ... more This study evaluated physical, chemical and morphological changes in lungs of mice infected with Paracoccidioides brasiliensis. The animals were inoculated with 0.1 mL of fungal suspension of the P. brasiliensis 18 isolate and were euthanized 1, 2, 4 and 8 weeks after inoculation. The upper left lobe of the lung was isolated, fixed and processed for paraffin embedding. The sections were stained with H&E for histopathological study, with Gomori-Grocott to locate and identify the fungus, and with TUNEL immunostaining to detect the occurrence of programmed cell death. The lower and middle right lobes were analyzed by Fourier Transform Infrared Photoacoustic Spectrocopy (FTIR-PAS) to investigate physical and chemical features of the infected lungs. The results showed that lungs infected by P. brasiliensis underwent structural changes that varied according to the time period analyzed, and that changes in the absorption bands of different chemical groups resulted from these morphological changes. The results suggest that the combination of FTIR-PAS spectroscopy with morphological evaluation is an effective procedure for the study of paracoccidioidomycosis, one of the most important systemic mycoses that can damage the lung architecture and consequently impair the respiratory function.

Journal of Biomedical Optics, 2013

We evaluate the biological and physicochemical effects of a Fusarium oxysporum crude extract (CE)... more We evaluate the biological and physicochemical effects of a Fusarium oxysporum crude extract (CE) on the skin of healthy rats. The CE is topically applied and subsequently the skin is collected after 3, 6, 12, and 24 h. The samples are analyzed by Fourier transform infrared photoacoustic spectroscopy (FTIR-PAS) and histomorphometric analysis. Terminal dUTP nick end labeling (TUNEL assay) is performed to detect both the cells in apoptosis and proliferation. There is a thickening of the epidermis after 6, 12, and 24 h and dermis after 12 and 24 h of CE application. A reduction of the dermis thickness is observed at 3 and 6 h. The treated skin shows higher labeling intensity by TUNEL at 3 h, while a higher intensity by proliferating cell nuclear antigen occurs at 3 and 12 h. FTIR-PAS data support the histology observations showing an increase in the absorption peaks in the dermis after the application of the CE. F. oxysporum CE permeated through the epidermis and the dermis, reaching the subcutaneous tissue, inducing cell apoptosis, and causing physicochemical changes in the organic molecules located in the dermis. This is the first known study associating histopathological and physical chemistry changes on healthy skin after the application of F. oxysporum CE.

Food Chemistry, 2014

Curcumin was complexed with β-CD using co-precipitation, freeze-drying and solvent evaporation me... more Curcumin was complexed with β-CD using co-precipitation, freeze-drying and solvent evaporation methods. Co-precipitation enabled complex formation, as indicated by the FT-IR and FT-Raman techniques via the shifts in the peaks that were assigned to the aromatic rings of curcumin. In addition, photoacoustic spectroscopy and X-ray diffraction, with the disappearance of the band related to aromatic rings, by Gaussian fitting, and modifications in the spectral lines, respectively, also suggested complex formation. The possible complexation had an efficiency of 74% and increased the solubility of the pure colourant 31-fold. Curcumin-β-CD complex exhibited a sunlight stability 18% higher than the pure colourant. This material was stable to pH variations and storage at -15 and 4°C. With an isothermal heating at 100 and 150°C for 2h, the material exhibited a colour retention of approximately 99%. The application of curcumin-β-CD complex in vanilla ice creams intensified the colour of the products and produced a great sensorial acceptance.

Analytical Chemistry, 1987

Journal of Dental Research, 2013

International Journal of Pharmaceutics, 2015

The pressure ulcer healing is a complex process and difficult to be achieved. Insulin is known to... more The pressure ulcer healing is a complex process and difficult to be achieved. Insulin is known to promote wound healing, and when complexed with cyclodextrin presents improved solubility, stability and biological activity. Complexation of insulin with hydroxypropyl-beta-cyclodextrin (HPβCD) was performed in this work through the coprecipitation method, providing the inclusion complex (HPβCD-I). The spectroscopic techniques used to analyze the complex were H(1) NMR, FT-Raman and FT-IR/ATR. A gel containing the HPβCD-I complex was prepared and a clinical study was conducted in patients with pressure ulcers. The spectroscopic techniques allowed to confirm the complex formation through the inclusion of aromatic amino acids, such as phenylalanine present in the HPβCD cavity. Data obtained from the FT-Raman and FT-IR/ATR techniques, combined with the H(1) NMR results, showed the effectiveness of these techniques in evaluating the inclusion complex of HPβCD with insulin. Clinical studies demonstrated tissue revitalization and a trend (p=0.06) for a significant difference between the healing effect of the control gel and that with HPβCD-I complex. The creation of the gel prepared with insulin and HPβCD-I complex and its use in patients with pressure ulcers appears to be promising in wound healing and its possible use in hospital care.

Journal of Esthetic and Restorative Dentistry, 2014

To evaluate the color match of different composite resins relative to Vitapan Classical shade gui... more To evaluate the color match of different composite resins relative to Vitapan Classical shade guide tab and their respective manufacturers' shade guide tabs as a function of time and storage. Three enamel shade A2 composite resins were used to fabricate 36 disk-shaped polymerized specimens (12 each), allocated into 2 groups of 6 and stored dry (GD) and in artificial saliva (GS). CIELAB coordinates from shade tabs and resin specimens immediately after polymerization (t0), and 24 hours (t1), 7 (t7), 14 (t14) and 21 (t21) days after polymerization were captured using a colorimeter. Color difference (ΔE00 ) between composite specimens and the reference tabs was calculated using the CIEDE2000 formula. The results were analyzed with repeated measures ANOVA, Tukey's HDS post-hoc test, and Student t test (p ≤ 0.05). Color of the three tested composites relative to Vita and their respective tabs significantly changed as a function of time until t14; however, between t14 and t21, no significant differences were found. No differences in color were found relative to storage at t14 and t21. ΔE00 values of specimens at t14 were significantly higher relative to their respective tabs than to Vitapan tab. For all brands color changed up to day 14, when it stabilized, regardless of whether composite specimens had been stored in artificial saliva or simply in a box. Vitapan tab presented a better color match than the manufacturers' tabs. The results found in this study demonstrated that the Vitapan Classical shade guide tab A2 provided a better color match than the respective shade guide tabs A2 supplied by the composite manufacturers. If custom shade tabs are to be made, however, they could be kept in a box and used as shade references from 14 days after being fabricated, when color stabilizes.

Journal of Raman Spectroscopy, 2014

Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, 2014

Photochemistry and Photobiology, 2013

This study evaluated physical, chemical and morphological changes in lungs of mice infected with ... more This study evaluated physical, chemical and morphological changes in lungs of mice infected with Paracoccidioides brasiliensis. The animals were inoculated with 0.1 mL of fungal suspension of the P. brasiliensis 18 isolate and were euthanized 1, 2, 4 and 8 weeks after inoculation. The upper left lobe of the lung was isolated, fixed and processed for paraffin embedding. The sections were stained with H&E for histopathological study, with Gomori-Grocott to locate and identify the fungus, and with TUNEL immunostaining to detect the occurrence of programmed cell death. The lower and middle right lobes were analyzed by Fourier Transform Infrared Photoacoustic Spectrocopy (FTIR-PAS) to investigate physical and chemical features of the infected lungs. The results showed that lungs infected by P. brasiliensis underwent structural changes that varied according to the time period analyzed, and that changes in the absorption bands of different chemical groups resulted from these morphological changes. The results suggest that the combination of FTIR-PAS spectroscopy with morphological evaluation is an effective procedure for the study of paracoccidioidomycosis, one of the most important systemic mycoses that can damage the lung architecture and consequently impair the respiratory function.

Journal of Biomedical Optics, 2013

We evaluate the biological and physicochemical effects of a Fusarium oxysporum crude extract (CE)... more We evaluate the biological and physicochemical effects of a Fusarium oxysporum crude extract (CE) on the skin of healthy rats. The CE is topically applied and subsequently the skin is collected after 3, 6, 12, and 24 h. The samples are analyzed by Fourier transform infrared photoacoustic spectroscopy (FTIR-PAS) and histomorphometric analysis. Terminal dUTP nick end labeling (TUNEL assay) is performed to detect both the cells in apoptosis and proliferation. There is a thickening of the epidermis after 6, 12, and 24 h and dermis after 12 and 24 h of CE application. A reduction of the dermis thickness is observed at 3 and 6 h. The treated skin shows higher labeling intensity by TUNEL at 3 h, while a higher intensity by proliferating cell nuclear antigen occurs at 3 and 12 h. FTIR-PAS data support the histology observations showing an increase in the absorption peaks in the dermis after the application of the CE. F. oxysporum CE permeated through the epidermis and the dermis, reaching the subcutaneous tissue, inducing cell apoptosis, and causing physicochemical changes in the organic molecules located in the dermis. This is the first known study associating histopathological and physical chemistry changes on healthy skin after the application of F. oxysporum CE.

Food Chemistry, 2014

Curcumin was complexed with β-CD using co-precipitation, freeze-drying and solvent evaporation me... more Curcumin was complexed with β-CD using co-precipitation, freeze-drying and solvent evaporation methods. Co-precipitation enabled complex formation, as indicated by the FT-IR and FT-Raman techniques via the shifts in the peaks that were assigned to the aromatic rings of curcumin. In addition, photoacoustic spectroscopy and X-ray diffraction, with the disappearance of the band related to aromatic rings, by Gaussian fitting, and modifications in the spectral lines, respectively, also suggested complex formation. The possible complexation had an efficiency of 74% and increased the solubility of the pure colourant 31-fold. Curcumin-β-CD complex exhibited a sunlight stability 18% higher than the pure colourant. This material was stable to pH variations and storage at -15 and 4°C. With an isothermal heating at 100 and 150°C for 2h, the material exhibited a colour retention of approximately 99%. The application of curcumin-β-CD complex in vanilla ice creams intensified the colour of the products and produced a great sensorial acceptance.

Analytical Chemistry, 1987

Journal of Dental Research, 2013