Mahdi Askari Badouei | Ferdowsi University of Mashhad (original) (raw)

Papers by Mahdi Askari Badouei

Frontiers in Veterinary Science

Avian pathogenic Escherichia coli (APEC) is an important bacterial pathogen that causes avian col... more Avian pathogenic Escherichia coli (APEC) is an important bacterial pathogen that causes avian colibacillosis and leads to huge economic losses in the poultry industry. Different virulence traits contribute to pathogenesis of APEC infections, and antimicrobial resistance (AMR) has also been an overwhelming issue in poultry worldwide. In the present study, we aimed to investigate and compare the presence of virulence-associated genes (VAGs), AMR, and phylogenetic group's distribution among APEC and avian fecal E. coli (AFEC) strains. E. coli from birds with colisepticemia and yolk sac infection (YSI) (APEC) plus E. coli strains from the feces of healthy birds (AFEC) were compared by the aforementioned traits. In addition, the clonal relatedness was compared using Enterobacterial repetitive intergenic consensus PCR (ERIC-PCR). Although all strains were susceptible to fosfomycin, ceftriaxone, and cefixime, almost all strains (98%) were multi-drug resistant (MDR). All strains (except...

BMC Research Notes, 2021

Objectives The emergence of colistin-resistant Enterobacteriaceae from human and animal sources i... more Objectives The emergence of colistin-resistant Enterobacteriaceae from human and animal sources is one of the major public health concerns as colistin is the last-resort antibiotic for treating infections caused by multidrug-resistant Gram-negative bacteria. We aimed to determine the prevalence of the prototype widespread colistin resistance genes (mcr-1 and mcr-2) among commensal and pathogenic Escherichia coli strains isolated from food-producing and companion animals in Iran. Results A total of 607 E. coli isolates which were previously collected from different animal sources between 2008 and 2016 used to uncover the possible presence of plasmid-mediated colistin resistance genes (mcr-1 and mcr-2) by PCR. Overall, our results could not confirm the presence of any mcr-1 or mcr-2 positive E. coli among the studied isolates. It is concluded that despite the important role of food-producing animals in transferring the antibiotic resistance, they were not the main source for carriage ...

Archives of Razi Institute, 2020

Bordetellosis or turkey coryza, caused by Bordetella avium, has been an issue for turkey industry... more Bordetellosis or turkey coryza, caused by Bordetella avium, has been an issue for turkey industry since its first description in 1967 when it was reported for the first time. Bordetella avium causes a highly contagious upper respiratory disease in turkeys. Therefore, this study aimed to isolate and characterize this species from commercial and backyard turkeys in Tehran, Isfahan, and Northern provinces of Iran. For the purpose of the study, 625 tracheal swabs were taken from 425 commercial poults and 200 backyard poults aged 2-6 weeks from September 2016 to September 2018. The swabs were immediately plated on MacConkey and blood agar plates and then pooled (5 swabs/pool) in tubes, containing 2 mL distilled water, to perform direct polymerase chain reaction (PCR) for the identification of B. avium. A total of 17 swab pools were found to be positive for B. avium. A subset of seven positive samples were sequenced for the flanking region of piuA gene. The analysis of the sequences indic...

BMC Veterinary Research

Background Transmission of antimicrobial resistant and virulent Escherichia coli (E. coli) from a... more Background Transmission of antimicrobial resistant and virulent Escherichia coli (E. coli) from animal to human has been considered as a public health concern. This study aimed to determine the phylogenetic background and prevalence of diarrheagenic E. coli and antimicrobial resistance in healthy riding-horses in Iran. In this research, the genes related to six main pathotypes of E. coli were screened. Also, genotypic and phenotypic antimicrobial resistance against commonly used antibiotics were studied, then phylo-grouping was performed on all the isolates. Results Out of 65 analyzed isolates, 29.23 % (n = 19) were determined as STEC and 6.15 % (n = 4) as potential EPEC. The most prevalent antimicrobial resistance phenotypes were against amoxicillin/clavulanic acid (46.2 %) and ceftriaxone (38.5 %). blaTEM was the most detected resistance gene (98.4 %) among the isolates and 26.15 % of the E. coli isolates were determined as multi-drug resistant (MDR). Three phylo-types including B...

Genotype and pathotype characterization of Escherichia coli isolates through molecular methods at... more Genotype and pathotype characterization of Escherichia coli isolates through molecular methods at a specific geographical area is particularly useful in the evaluation of disease, epidemiology. Enteropathogenic Escherichia coli (EPEC) and shigatoxin-producing Escherichia coli (STEC) are among the most important E. coli pathotypes. In this study 80 fecal samples were collected from 11 healthy sheep flocks in winter and spring of 2011 in Garmsar district. Escherichia coli isolates were screened by multiplex-PCR for stx1, stx2, eae, and ehly virulence genes. Among fecal samples, 47 cases (58/75%) were diagnosed as STEC Carrier. Among 98 E. coli isolates, stx1/ehly (54/8%) was the predominant genotype. EPEC strains were not recognized in this study. Therefore, sheep might be a possible reservoir for STEC infection in Garmsar and can be transmitted to humans through consumption of lamb meat and other related products.

Iranian Journal of Basic Medical Sciences, 2020

Objective(s): The enteroaggregative Escherichia coli (EAEC) has been one of the most intriguing e... more Objective(s): The enteroaggregative Escherichia coli (EAEC) has been one of the most intriguing emerging bacterial pathogens in children that occur both in developing countries and the industrial world. Although various phenotypic and genotypic based protocols have been suggested for diagnosis of EAEC, they are not conclusive or practical to be used in most clinical laboratories. Materials and Methods: In this study, we analyzed and compared 36 typical EAEC strains (aggR-positive) by various genotypic and phenotypic methods. Results: Briefly, pCVD432 was detected in all of isolates along with aggR, then it was followed by other virulence genes including app, astA, aggA, and pet genes in 32 (88.8%), 21 (58.3%), 9 (25%), and 2 (5.5%) isolates, respectively. Biofilm was formed by 34 (94.4%) isolates, while only 26 (72.2%) isolates showed an aggregative adherence pattern to HEp-2 cells. Conclusion: The genetic and phenotypic features of EAEC were highly inconsistent, which may have cons...

Bulgarian Journal of Veterinary Medicine, 2021

M. JAJARMI, M. ASKARI BADOUEI, R. GHANBARPOUR, A. KARMOSTAJI & H. ALIZADE Department of Pathobiol... more M. JAJARMI, M. ASKARI BADOUEI, R. GHANBARPOUR, A. KARMOSTAJI & H. ALIZADE Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran; Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran; Infectious and Tropical Disease Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran; Student Research Committee, Hormozgan University of Medical Sciences, Bandar Abbas, Iran; Research Center for Tropical and Infectious Diseases, Kerman University of Medical Sciences, Kerman, Iran

BMC veterinary research, Jan 16, 2018

All over the world, Shiga toxin-producing Escherichia coli (STEC) are considered as important zoo... more All over the world, Shiga toxin-producing Escherichia coli (STEC) are considered as important zoonotic pathogens. Eight serogroups have the greatest role in the outbreaks and diseases caused by STEC which include O26, O45, O103, O111, O113, O121, O145 and O157. Ruminants, especially cattle are the main reservoirs but the role of small ruminants in the epidemiology of human infections has not been thoroughly assessed in many countries. The objective of this research was to investigate the pathogenic potential of the STEC strains isolated from slaughtered goats. In this study, a total of 57 STEC strains were recovered from 450 goats and characterized by subtyping of stx genes, O-serogrouping, phylo-typing and DNA fingerprinting. Amongst 57 STEC strains isolated from goats, the prevalence of stx1 was significantly more than stx2 (98.2% vs. 24.5%; P ≤ 0.05), and 22.8% of strains harbored both stx1 and stx2 genes. Three (5.2%) isolates were characterized as EHEC, which carried both eae a...

Background Subtilase is a potent cytotoxin that was first described in O113:H21 strain in Austral... more Background Subtilase is a potent cytotoxin that was first described in O113:H21 strain in Australia as a plasmid- encoded cytotoxin (subAB1). Subsequently, chromosomal variants including subAB2-1, subAB2-2, and subAB2-3 were described. Results In the present study a collection of 101 archived STEC strains isolated from various sources in Iran (2009–2016) were analyzed for the detection of different genes encoding the subtilase variants, plasmidic and chromosomal virulence genes, together with the phylogroup and serogroups. Overall, 57 isolates (56.4%) carried at least one variant of subAB. Most strains from small ruminants including 93% of sheep and 96% of caprine isolates carried at least one chromosomally encoded variant (subAB-2-1 and/or subAb2-2). In contrast, 12 cattle isolates (24%) only harbored the plasmid encoded variant (subAB1). STEC strains from other sources including deer, pony and humans were positive for subAB-2-1 and/or subAb2-2. Concerning the virulence markers, so...

Most efforts to elucidate virulence mechanisms of mammary pathogenic Escherichia coli (MPEC), cau... more Most efforts to elucidate virulence mechanisms of mammary pathogenic Escherichia coli (MPEC), causative agent of bovine clinical mastitis, have been failed but some recent studies introduced iron acquisition systems as major role players in pathogenicity. Here, we investigated the different iron uptake systems genotypes and assessed how they relate to virulence potential of MPEC. In total, 217 E. coli isolates (MPEC= 157, fecal isolates= 60) were screened for the presence of nine genes related to iron acquisition (iroN, iutA, fecA, fyuA, sitA, irp2, iucD, chuA and tonB) and phylogenetic groups were also determined. Next, bacterial growth potential and survival in raw and UHT milk which are representative for crucial steps in mastitis development were evaluated. In addition, the mineral consumption of E. coli cultured in milk were measured. The results showed that MPEC strains considerably tend to possess fecA (93%, p= 0.000) and belong to phylogenetic group A (42%, p= 0.042). The fe...

Iranian Journal of Microbiology

Background and Objectives: Escherichia coli is responsible for various enteric and extraintestina... more Background and Objectives: Escherichia coli is responsible for various enteric and extraintestinal infections in animals and humans. Iron as an essential nutrient, has a proven role in pathogenicity of E. coli. Pathogenic E. coli benefits of having complicated systems for iron acquisition but our current knowledge is limited because of complexity of these systems. In the present study, three multiplex-PCR assays were developed to screen nine different virulence genes related to diverse iron acquisition systems in E. coli. Materials and Methods: The multiplex-PCR systems were designed and optimized in three panels. Each panel includes a triplex-PCR cocktail. The panels are as follow: panel 1: iroN, iutA and fecA; panel 2: fyuA, sitA and irp2; and panel 3: iucD, chuA and tonB. A total of 39 pathogenic E. coli was screened according to the designed multiplex-PCR. Results: In total, the top three frequent genes were tonB (100%), fecA (66.6%) and sitA (58.9%). With the exception of fecA ...

Comparative Clinical Pathology

Journal of Global Antimicrobial Resistance

Microbial pathogenesis, 2017

The aim of this study was to investigate the virulence potential of the isolated bovine STEC for ... more The aim of this study was to investigate the virulence potential of the isolated bovine STEC for humans in Iran. In this study a collection of STEC strains (n = 50) had been provided via four stages, including sampling from feces of cattle, E. coli isolation, molecular screening of Shiga toxin (stx) genes, and saving the STEC strains from various geographical areas in Iran. The STEC isolates were subjected to stx-subtyping, O-serogrouping, and phylo-grouping by conventional polymerase chain reaction (PCR). Occurrence of stx1 (52%) and stx2 (64%) was not significantly different (p = 0.1), and 16% of isolates carried both stx1 and stx2, simultaneously. In addition, 36% and 80% of the isolates were positive for eae and ehxA, respectively. Molecular subtyping showed that stx1a (52%), stx2a (44%), stx2c (44%), and stx2d (30%) were the most prevalent subtypes; two combinations stx2a/stx2c and stx2c/stx2d coexisted in 18% and 10% of STEC strains, respectively. Three important non-O157 sero...