ALOYSIUS MATTE | Mbarara University of Science and Technology (MUST) (original) (raw)
Papers by ALOYSIUS MATTE
Pan African Medical Journal, 2017
IntroductionWhile the laboratory represents more than 70% of clinical diagnosis and patient manag... more IntroductionWhile the laboratory represents more than 70% of clinical diagnosis and patient management, access to reliable and quality laboratory diagnostics in sub-Saharan Africa remains a challenge. To gain knowledge and suggest evidence based interventions towards laboratory improvement in Southwestern Uganda, we assessed the baseline laboratory quality standards in three medical and research laboratories in Southwestern Uganda.MethodsWe conducted a cross sectional survey from October, 2013 to April, 2014. Selected laboratories, including one private research, one private for profit and one public laboratory, were assessed using the WHO AFRO_SLIPTA checklist and baseline scores were determined.ResultsThe three laboratories assessed met basic facility requirements, had trained personnel, and safety measures in place. Sample reception was properly designed and executed with a well designated chain of custody. All laboratories had sufficient equipment for the nature of work they were involved in. However, we found that standard operating procedures were incomplete in all three laboratories, lack of quality audit schemes by two laboratories and only one laboratory enrolled into external quality assurance schemes. The SLIPTA scores were one star for the research laboratory and no star for both the public and private-for-profit laboratories.ConclusionWhile most of the laboratory systems were in place, the low scores obtained by the assessed laboratories reflect the need for improvement to reach standards of quality assured diagnostics in the region. Therefore, routine mentorship and regional supportive supervision are necessary to increase the quality of laboratory services.
Advances in Infectious Diseases, 2013
Background: Screening blood donors has practically eliminated viral and bacterial pathogens in bl... more Background: Screening blood donors has practically eliminated viral and bacterial pathogens in blood used for transfusion. However, transfusion-associated bacterial sepsis remains an important health-care concern and the commonest cause of transfusion-related fatality in resource limited settings. Data on bacterial contamination of blood are scarce while the demand of blood transfusion is continuously growing. Therefore we conducted a study to determine the prevalence and type of bacterial contamination in donor blood and blood products, at the Mbarara Regional Blood Bank. Methodology: A total of 510 units of screened blood and blood products consisting of refrigerated whole blood and packed cells were randomly sampled following aseptic procedures from Mbarara Regional Blood Bank. Two samples from each unit were collected in universal containers containing Brain Heart Infusion Broth and incubated at 37˚C for up to 7 days. Subcultures were carried out on Blood agar, Chocolate agar and MacConkey agar. Isolates were identified by standard microbiologic techniques and drug susceptibility testing was performed by Kirby Bauer disc diffusion method. Results: Of the 510 samples collected between June and October 2012, 18 (3.5%) samples showed growth. The contaminants were Staphylococcus aureus 17/18 (94.4%) and Streptococcus viridans 1/18 (5.6%). Isolates were sensitive to erythomycin, ampicillin, chloramphenicol and ciprofloxacin and resistant to penicillin and cloxacillin. Conclusion: Blood and blood products from Mbarara Regional Blood have unacceptable levels of bacterial contamination that can affect patient safety especially in an area with high malaria endemicity. Therefore it is critical to improve hygiene precautions in order to minimize bacterial contamination and ensure patient safety.
Background: Screening blood donors has practically eliminated viral and bacterial pathogens in bl... more Background: Screening blood donors has practically eliminated viral and bacterial pathogens in blood used for transfusion. However, transfusion-associated bacterial sepsis remains an important health-care concern and the commonest cause of transfusion-related fatality in resource limited settings. Data on bacterial contamination of blood are scarce while the demand of blood transfusion is continuously growing. Therefore we conducted a study to determine the prevalence and type of bacterial contamination in donor blood and blood products, at the Mbarara Regional Blood Bank. Methodology: A total of 510 units of screened blood and blood products consisting of refrigerated whole blood and packed cells were randomly sampled following aseptic procedures from Mbarara Regional Blood Bank. Two samples from each unit were collected in universal containers containing Brain Heart Infusion Broth and incubated at 37˚C for up to 7 days. Subcultures were carried out on Blood agar, Chocolate agar and MacConkey agar. Isolates were identified by standard microbiologic techniques and drug susceptibility testing was performed by Kirby Bauer disc diffusion method. Results: Of the 510 samples collected between June and October 2012, 18 (3.5%) samples showed growth. The contaminants were Staphylococcus aureus 17/18 (94.4%) and Streptococcus viridans 1/18 (5.6%). Isolates were sensitive to erythomycin, ampicillin, chloramphenicol and ciprofloxacin and resistant to penicillin and cloxacillin. Conclusion: Blood and blood products from Mbarara Regional Blood have unacceptable levels of bacterial contamination that can affect patient safety especially in an area with high malaria endemicity. Therefore it is critical to improve hygiene precautions in order to minimize bacterial contamination and ensure patient safety.
Pan African Medical Journal, 2017
IntroductionWhile the laboratory represents more than 70% of clinical diagnosis and patient manag... more IntroductionWhile the laboratory represents more than 70% of clinical diagnosis and patient management, access to reliable and quality laboratory diagnostics in sub-Saharan Africa remains a challenge. To gain knowledge and suggest evidence based interventions towards laboratory improvement in Southwestern Uganda, we assessed the baseline laboratory quality standards in three medical and research laboratories in Southwestern Uganda.MethodsWe conducted a cross sectional survey from October, 2013 to April, 2014. Selected laboratories, including one private research, one private for profit and one public laboratory, were assessed using the WHO AFRO_SLIPTA checklist and baseline scores were determined.ResultsThe three laboratories assessed met basic facility requirements, had trained personnel, and safety measures in place. Sample reception was properly designed and executed with a well designated chain of custody. All laboratories had sufficient equipment for the nature of work they were involved in. However, we found that standard operating procedures were incomplete in all three laboratories, lack of quality audit schemes by two laboratories and only one laboratory enrolled into external quality assurance schemes. The SLIPTA scores were one star for the research laboratory and no star for both the public and private-for-profit laboratories.ConclusionWhile most of the laboratory systems were in place, the low scores obtained by the assessed laboratories reflect the need for improvement to reach standards of quality assured diagnostics in the region. Therefore, routine mentorship and regional supportive supervision are necessary to increase the quality of laboratory services.
Advances in Infectious Diseases, 2013
Background: Screening blood donors has practically eliminated viral and bacterial pathogens in bl... more Background: Screening blood donors has practically eliminated viral and bacterial pathogens in blood used for transfusion. However, transfusion-associated bacterial sepsis remains an important health-care concern and the commonest cause of transfusion-related fatality in resource limited settings. Data on bacterial contamination of blood are scarce while the demand of blood transfusion is continuously growing. Therefore we conducted a study to determine the prevalence and type of bacterial contamination in donor blood and blood products, at the Mbarara Regional Blood Bank. Methodology: A total of 510 units of screened blood and blood products consisting of refrigerated whole blood and packed cells were randomly sampled following aseptic procedures from Mbarara Regional Blood Bank. Two samples from each unit were collected in universal containers containing Brain Heart Infusion Broth and incubated at 37˚C for up to 7 days. Subcultures were carried out on Blood agar, Chocolate agar and MacConkey agar. Isolates were identified by standard microbiologic techniques and drug susceptibility testing was performed by Kirby Bauer disc diffusion method. Results: Of the 510 samples collected between June and October 2012, 18 (3.5%) samples showed growth. The contaminants were Staphylococcus aureus 17/18 (94.4%) and Streptococcus viridans 1/18 (5.6%). Isolates were sensitive to erythomycin, ampicillin, chloramphenicol and ciprofloxacin and resistant to penicillin and cloxacillin. Conclusion: Blood and blood products from Mbarara Regional Blood have unacceptable levels of bacterial contamination that can affect patient safety especially in an area with high malaria endemicity. Therefore it is critical to improve hygiene precautions in order to minimize bacterial contamination and ensure patient safety.
Background: Screening blood donors has practically eliminated viral and bacterial pathogens in bl... more Background: Screening blood donors has practically eliminated viral and bacterial pathogens in blood used for transfusion. However, transfusion-associated bacterial sepsis remains an important health-care concern and the commonest cause of transfusion-related fatality in resource limited settings. Data on bacterial contamination of blood are scarce while the demand of blood transfusion is continuously growing. Therefore we conducted a study to determine the prevalence and type of bacterial contamination in donor blood and blood products, at the Mbarara Regional Blood Bank. Methodology: A total of 510 units of screened blood and blood products consisting of refrigerated whole blood and packed cells were randomly sampled following aseptic procedures from Mbarara Regional Blood Bank. Two samples from each unit were collected in universal containers containing Brain Heart Infusion Broth and incubated at 37˚C for up to 7 days. Subcultures were carried out on Blood agar, Chocolate agar and MacConkey agar. Isolates were identified by standard microbiologic techniques and drug susceptibility testing was performed by Kirby Bauer disc diffusion method. Results: Of the 510 samples collected between June and October 2012, 18 (3.5%) samples showed growth. The contaminants were Staphylococcus aureus 17/18 (94.4%) and Streptococcus viridans 1/18 (5.6%). Isolates were sensitive to erythomycin, ampicillin, chloramphenicol and ciprofloxacin and resistant to penicillin and cloxacillin. Conclusion: Blood and blood products from Mbarara Regional Blood have unacceptable levels of bacterial contamination that can affect patient safety especially in an area with high malaria endemicity. Therefore it is critical to improve hygiene precautions in order to minimize bacterial contamination and ensure patient safety.