Zoltán Péterfi | University of Pécs (original) (raw)
Papers by Zoltán Péterfi
Journal of Immunoassay, 2000
ELISA is a sensitive, specific, reproducible and fast method for detection of antigen-antibody re... more ELISA is a sensitive, specific, reproducible and fast method for detection of antigen-antibody reactions. In case of non-protein antigens as LPS, problems exist, such as poor proportion of coating to microplates, non-specific binding of antibodies to the plastic wells. These problems were resolved partially by Takahashi and co-workers using poly-L-lysine for coating of LPS antigens. To reduce non-specific binding, blocking agent, such as bovine serum albumin (BSA) or casein is commonly used. We have to choose the blocking agent carefully because LPS can bind proteins non-specifically. This process can inhibit binding of LPS-specific antibody to LPS and decrease the sensitivity of method. In this paper we describe an ELISA test for LPS in which normal goat serum is used for blocking. This modification increases the sensitivity of ELISA. This method is useful for detection of LPS (S, R form) and anti-LPS antibody reaction in serological cross-reaction studies.
Orvosi Hetilap
The leptospirosis and the hantaviral infections are worldwide distributed zoonoses having the sim... more The leptospirosis and the hantaviral infections are worldwide distributed zoonoses having the similar epidemiology and clinical symptoms. Both in Europe and Asia those hantaviral serotypes are common which are responsible for the haemorrhagic fever with renal syndrome, while on the American continent the hantaviral pulmonary syndrome has also been diagnosed. Authors describe a patient who had simultaneous leptospira and hantaviral infections with haemorrhagic fever as well as with mild, transient renal insufficiency and liver damage. The dual infection was proved by serology.
ELECTROPHORESIS, 2015
The structure of the oligosaccharide repeating units of endotoxins from Gram-negative bacteria is... more The structure of the oligosaccharide repeating units of endotoxins from Gram-negative bacteria is characteristic for the different serogroups and serotypes of bacteria. Detailed examination of the cross-reactions of three enterobacterial serotypes, Proteus morganii O34, Escherichia coli O111 and Salmonella enterica sv. Adelaide O35, was performed using sensitive tests (ELISA, immunoblotting). Fine differences between the endotoxins of the bacteria were detected using silver staining of SDS-PAGE gels and chip-technology for the intact lipopolysaccharides. The compositions of the O-specific polysaccharides of lipopolysaccharides extracted from the bacteria were studied, and it was proven that the three cross-reacting bacteria contain O-antigens built from the same monosaccharides, namely colitoses linked to glucose, galactose and N-acetyl-galactosamine. The NMR and GC-MS studies revealed that the most probable component for the cross-reaction is the rare sugar, colitose. The composition of the lipid A parts of the LPSs were found to be identical by ESI-MS measurements. This article is protected by copyright. All rights reserved.
Orvosi Hetilap, 2014
During the past years a dramatic change has been observed in the epidemiology of Clostridium diff... more During the past years a dramatic change has been observed in the epidemiology of Clostridium difficile infections. The aim of the authors was to investigate the possibility of the fecal microbiota transplantation and study differences, if any, in the success rate of the two different upper gastrointestinal tract method. 100 ml of fecal microbiota solution was instilled via a nasoduodenal tube in 15 cases and a nasogastric tube in 15 cases. The authors defined the primary cure rate as the percentage of cases in which the symptoms disappeared without recurrence within 6 weeks after the first fecal microbiota transplantation, while secondary cure rate was calculated as the percentage of cases in which the symptoms resolved after the second fecal microbiota transplantation. It was found that fecal microbiota transplantation applied via the nasoduodenal tube resulted in a 100% primary cure rate. With the use of the nasogastric tube, the primary and secondary cure rate were 80% and 93.3%, respectively. Fecal microbiota transplantation via the upper gastrointestinal tract was found to have an overall primary cure rate of 90.0% and a secondary cure rate of 96.7%. Fecal microbiota transplantation proved to be very effective, particularly in recurrent infections and cases where conventional treatment failed.
Health, 2011
Background: Visceral leishmaniasis caused by L. infantum Nicolle 1908 (Kinetoplastida, Trypanosom... more Background: Visceral leishmaniasis caused by L. infantum Nicolle 1908 (Kinetoplastida, Trypanosomatidae) is very rare in immunocompetent adults. Clinical manifestation of the infection occurs in children and immune-compromised patients associated with AIDS, haemopoietic malignancies or after renal, liver, and heart transplantations in Europe. Hungary is regarded free of leishmaniasis. Except for a single infection in a small girl without a travel history, only a few imported cases have been recorded among Hungarians returning from endemic areas. Case presentation: Visceral leishmaniasis was diagnosed in a 32 years old immumocompetent Hungarian man who had spent his holidays during the previous 3 years in Dalmatian territories (Dubrovnik, Makarska and Trogir) of Croatia. He had two months history of fever, chills, and night sweating associated with weight loss. Physical examination showed mild hepatomegaly and extreme splenomegaly. The protozoon infection was confirmed by parasitological, serological and molecular biological methods. Clinical recovery of the patient was observed after treatment with amphotericin B. Conclusion: We want to highlight the effects of climate changes and to call attention of health care professionals in Central and Eastern European countries to consider the possibility of leishmaniasis as an emerging disease of tourists returned from endemic regions.
Zeitschrift für Gastroenterologie, 2013
Zeitschrift für Gastroenterologie, 2010
Journal of Chromatography A, 2008
A fast microchip electrophoresis method was developed to analyze and differentiate bacterial endo... more A fast microchip electrophoresis method was developed to analyze and differentiate bacterial endotoxins directly from whole-cell lysates after removal of the proteinaceous components with proteinase K digestion and a precipitation of the endotoxin components. The partially purified endotoxin components were visualized by the interaction with dodecyl sulphate and then a fluorescent dye. The lipopolysaccharide (LPS) profiles can be directly evaluated from digested bacterial cells, and the electrophoresis patterns very closely resembled to those of pure LPSs, and the R and S chemotypes can be used to assign the strains. The method has been found to be useful in the screening of a large number of bacterial mutants and the structural characterization of endotoxins extracted only from 1 ml cultures.
Journal of Chromatography A, 2007
Bacterial strains have complex and individual antigenic structure, which provides basis for their... more Bacterial strains have complex and individual antigenic structure, which provides basis for their serological identification. However, serological cross-reaction may occur when antibodies against a certain strain recognize other strains too. The molecular basis of this phenomenon is the expression of similar or identical antigenic epitopes on the surface of different bacterial cells. Such cross-reactions might harden the serological diagnosis of pathogenic bacteria. But it can be also advantageous, when antigens of non-pathogenic strains can be used in the serological examinations. Serological cross-reaction between three taxonomically different strains -Proteus morganii O34 (8662/64), Escherichia coli O111 and Salmonella Adelaide O35 -have been described. It has been proven that it is based partially on the similar lipopolysaccharide structures of these pathogens. In this study the involvement of the outer membrane proteins of these strains in the serological cross-reaction is presented. Microfluidic chip technology was applied for the detection of common proteins, which provided fast and quantitative data about the proteins that might be responsible for serological cross-reaction. Two outer membrane proteins with apparent molecular mass of 36 and 41 kDa, respectively, could be detected in the profile of each strain, while individual dominating protein peaks have also appeared in the protein profiles. The presence of common protein antigens was proven by Western blotting.
Clinical and Experimental Medical Journal, 2011
Background: Since the clearance of hepatitis C virus (HCV) infection depends on the cytokines whi... more Background: Since the clearance of hepatitis C virus (HCV) infection depends on the cytokines which are under genetic control, we have studied genetic polymorphisms of two pro-inflammatory interleukin-28B (IL-28B) (also named as interferon λ-3) and lymphotoxin-A (LT-A) as well ...
Emerging Infectious Diseases, 2004
ELECTROPHORESIS, 2012
A novel, fast, and sensitive ME method was developed to analyze and differentiate the smooth (S) ... more A novel, fast, and sensitive ME method was developed to analyze and differentiate the smooth (S) and rough (R) type bacterial endotoxin components labeled covalently with a fluorescent dye. The quantitative analysis of purified lipopolysaccharides, or partially purified samples from whole-cell lysates becomes possible with this method. Two groups with three sub-groups in the first group of S-type lipopolysaccharides can be classified based on the electrophoretic profiles. The LOD of the endotoxins from S- and R-type Gram-negative bacteria was found to be 2.6 ng and 6.9 ng, respectively. This method is capable to replace the commonly used SDS-PAGE combined with silver staining.
APMIS, 2006
In acute appendicitis the bowel transmissibility of the intestinal flora increases in relation to... more In acute appendicitis the bowel transmissibility of the intestinal flora increases in relation to inflammation and edema formation. We can therefore observe an immunologic response in patients, which is detectable using different bacteria isolated from the normal intestinal flora. Our aim was to measure this immunologic reaction and to detect the relationship between this response and histologic types of acute appendicitis. Sera from 47 cases, comprising 38 patients suffering from appendicitis and 9 healthy controls, were examined. The sera were taken shortly before appendectomy and 14 days after operation. The antigens were lipopolysaccharides (LPS) extracted from bacteria of normal intestinal flora: Escherichia coli O21, O22, O33, O61, O68, Bacteroides fragilis and an absolute rough mutant: Shigella sonnei Re 4350. Antibodies were detected by ELISA. We showed a direct relationship between severity of appendicitis and registered antibody titer. Both aerobic and anaerobic bacteria play a role in infection in appendicitis. According to our serologic results the synergy of B. fragilis with E. coli from normal flora is more important in the initiation of inflammation, but in the perforation process the role of E. coli seems more important compared to that of B. fragilis.
Acta Microbiologica et Immunologica Hungarica, 2011
Binding of fluorescein isothiocyanate-labeled concanavalin A to a series of molecular species of ... more Binding of fluorescein isothiocyanate-labeled concanavalin A to a series of molecular species of lipopolysaccharide (LPS), purified from pathogenic bacteria, was studied via agarose gel precipitation experiments and the results were compared with available structural data.
Diagnostic Microbiology and Infectious Disease, 2014
Hantaviruses, one of the causative agents of viral hemorrhagic fevers, represent a considerable h... more Hantaviruses, one of the causative agents of viral hemorrhagic fevers, represent a considerable healthcare threat. In Hungary, Dobrava-Belgrade virus (DOBV) and Puumala virus (PUUV) are the main circulating hantavirus species, responsible for the clinical picture known as hemorrhagic fever with renal syndrome, a disease that may be accompanied by acute kidney injury (AKI), requiring hospitalization with occasionally prolonged recovery phase. A total of 20 patient sera were collected over a 2-year period from persons hospitalized with AKI, displaying clinical signs and laboratory findings directly suggestive for hantavirus infection. Samples were tested using an immunoblot assay, based on complete viral nucleocapsid proteins to detect patients' IgM and IgG antibodies against DOBV and PUUV. In parallel, all specimens were also tested by 1-step real-time TaqMan reverse-transcriptase polymerase chain reaction to confirm infection and to determine the causative hantavirus genotype. We present here the first Hungarian clinical study spanning across 2 years and dedicated specifically to assess acute kidney injuries, in the context of hantavirus prevalence.
Journal of Immunoassay, 2000
ELISA is a sensitive, specific, reproducible and fast method for detection of antigen-antibody re... more ELISA is a sensitive, specific, reproducible and fast method for detection of antigen-antibody reactions. In case of non-protein antigens as LPS, problems exist, such as poor proportion of coating to microplates, non-specific binding of antibodies to the plastic wells. These problems were resolved partially by Takahashi and co-workers using poly-L-lysine for coating of LPS antigens. To reduce non-specific binding, blocking agent, such as bovine serum albumin (BSA) or casein is commonly used. We have to choose the blocking agent carefully because LPS can bind proteins non-specifically. This process can inhibit binding of LPS-specific antibody to LPS and decrease the sensitivity of method. In this paper we describe an ELISA test for LPS in which normal goat serum is used for blocking. This modification increases the sensitivity of ELISA. This method is useful for detection of LPS (S, R form) and anti-LPS antibody reaction in serological cross-reaction studies.
Orvosi Hetilap
The leptospirosis and the hantaviral infections are worldwide distributed zoonoses having the sim... more The leptospirosis and the hantaviral infections are worldwide distributed zoonoses having the similar epidemiology and clinical symptoms. Both in Europe and Asia those hantaviral serotypes are common which are responsible for the haemorrhagic fever with renal syndrome, while on the American continent the hantaviral pulmonary syndrome has also been diagnosed. Authors describe a patient who had simultaneous leptospira and hantaviral infections with haemorrhagic fever as well as with mild, transient renal insufficiency and liver damage. The dual infection was proved by serology.
ELECTROPHORESIS, 2015
The structure of the oligosaccharide repeating units of endotoxins from Gram-negative bacteria is... more The structure of the oligosaccharide repeating units of endotoxins from Gram-negative bacteria is characteristic for the different serogroups and serotypes of bacteria. Detailed examination of the cross-reactions of three enterobacterial serotypes, Proteus morganii O34, Escherichia coli O111 and Salmonella enterica sv. Adelaide O35, was performed using sensitive tests (ELISA, immunoblotting). Fine differences between the endotoxins of the bacteria were detected using silver staining of SDS-PAGE gels and chip-technology for the intact lipopolysaccharides. The compositions of the O-specific polysaccharides of lipopolysaccharides extracted from the bacteria were studied, and it was proven that the three cross-reacting bacteria contain O-antigens built from the same monosaccharides, namely colitoses linked to glucose, galactose and N-acetyl-galactosamine. The NMR and GC-MS studies revealed that the most probable component for the cross-reaction is the rare sugar, colitose. The composition of the lipid A parts of the LPSs were found to be identical by ESI-MS measurements. This article is protected by copyright. All rights reserved.
Orvosi Hetilap, 2014
During the past years a dramatic change has been observed in the epidemiology of Clostridium diff... more During the past years a dramatic change has been observed in the epidemiology of Clostridium difficile infections. The aim of the authors was to investigate the possibility of the fecal microbiota transplantation and study differences, if any, in the success rate of the two different upper gastrointestinal tract method. 100 ml of fecal microbiota solution was instilled via a nasoduodenal tube in 15 cases and a nasogastric tube in 15 cases. The authors defined the primary cure rate as the percentage of cases in which the symptoms disappeared without recurrence within 6 weeks after the first fecal microbiota transplantation, while secondary cure rate was calculated as the percentage of cases in which the symptoms resolved after the second fecal microbiota transplantation. It was found that fecal microbiota transplantation applied via the nasoduodenal tube resulted in a 100% primary cure rate. With the use of the nasogastric tube, the primary and secondary cure rate were 80% and 93.3%, respectively. Fecal microbiota transplantation via the upper gastrointestinal tract was found to have an overall primary cure rate of 90.0% and a secondary cure rate of 96.7%. Fecal microbiota transplantation proved to be very effective, particularly in recurrent infections and cases where conventional treatment failed.
Health, 2011
Background: Visceral leishmaniasis caused by L. infantum Nicolle 1908 (Kinetoplastida, Trypanosom... more Background: Visceral leishmaniasis caused by L. infantum Nicolle 1908 (Kinetoplastida, Trypanosomatidae) is very rare in immunocompetent adults. Clinical manifestation of the infection occurs in children and immune-compromised patients associated with AIDS, haemopoietic malignancies or after renal, liver, and heart transplantations in Europe. Hungary is regarded free of leishmaniasis. Except for a single infection in a small girl without a travel history, only a few imported cases have been recorded among Hungarians returning from endemic areas. Case presentation: Visceral leishmaniasis was diagnosed in a 32 years old immumocompetent Hungarian man who had spent his holidays during the previous 3 years in Dalmatian territories (Dubrovnik, Makarska and Trogir) of Croatia. He had two months history of fever, chills, and night sweating associated with weight loss. Physical examination showed mild hepatomegaly and extreme splenomegaly. The protozoon infection was confirmed by parasitological, serological and molecular biological methods. Clinical recovery of the patient was observed after treatment with amphotericin B. Conclusion: We want to highlight the effects of climate changes and to call attention of health care professionals in Central and Eastern European countries to consider the possibility of leishmaniasis as an emerging disease of tourists returned from endemic regions.
Zeitschrift für Gastroenterologie, 2013
Zeitschrift für Gastroenterologie, 2010
Journal of Chromatography A, 2008
A fast microchip electrophoresis method was developed to analyze and differentiate bacterial endo... more A fast microchip electrophoresis method was developed to analyze and differentiate bacterial endotoxins directly from whole-cell lysates after removal of the proteinaceous components with proteinase K digestion and a precipitation of the endotoxin components. The partially purified endotoxin components were visualized by the interaction with dodecyl sulphate and then a fluorescent dye. The lipopolysaccharide (LPS) profiles can be directly evaluated from digested bacterial cells, and the electrophoresis patterns very closely resembled to those of pure LPSs, and the R and S chemotypes can be used to assign the strains. The method has been found to be useful in the screening of a large number of bacterial mutants and the structural characterization of endotoxins extracted only from 1 ml cultures.
Journal of Chromatography A, 2007
Bacterial strains have complex and individual antigenic structure, which provides basis for their... more Bacterial strains have complex and individual antigenic structure, which provides basis for their serological identification. However, serological cross-reaction may occur when antibodies against a certain strain recognize other strains too. The molecular basis of this phenomenon is the expression of similar or identical antigenic epitopes on the surface of different bacterial cells. Such cross-reactions might harden the serological diagnosis of pathogenic bacteria. But it can be also advantageous, when antigens of non-pathogenic strains can be used in the serological examinations. Serological cross-reaction between three taxonomically different strains -Proteus morganii O34 (8662/64), Escherichia coli O111 and Salmonella Adelaide O35 -have been described. It has been proven that it is based partially on the similar lipopolysaccharide structures of these pathogens. In this study the involvement of the outer membrane proteins of these strains in the serological cross-reaction is presented. Microfluidic chip technology was applied for the detection of common proteins, which provided fast and quantitative data about the proteins that might be responsible for serological cross-reaction. Two outer membrane proteins with apparent molecular mass of 36 and 41 kDa, respectively, could be detected in the profile of each strain, while individual dominating protein peaks have also appeared in the protein profiles. The presence of common protein antigens was proven by Western blotting.
Clinical and Experimental Medical Journal, 2011
Background: Since the clearance of hepatitis C virus (HCV) infection depends on the cytokines whi... more Background: Since the clearance of hepatitis C virus (HCV) infection depends on the cytokines which are under genetic control, we have studied genetic polymorphisms of two pro-inflammatory interleukin-28B (IL-28B) (also named as interferon λ-3) and lymphotoxin-A (LT-A) as well ...
Emerging Infectious Diseases, 2004
ELECTROPHORESIS, 2012
A novel, fast, and sensitive ME method was developed to analyze and differentiate the smooth (S) ... more A novel, fast, and sensitive ME method was developed to analyze and differentiate the smooth (S) and rough (R) type bacterial endotoxin components labeled covalently with a fluorescent dye. The quantitative analysis of purified lipopolysaccharides, or partially purified samples from whole-cell lysates becomes possible with this method. Two groups with three sub-groups in the first group of S-type lipopolysaccharides can be classified based on the electrophoretic profiles. The LOD of the endotoxins from S- and R-type Gram-negative bacteria was found to be 2.6 ng and 6.9 ng, respectively. This method is capable to replace the commonly used SDS-PAGE combined with silver staining.
APMIS, 2006
In acute appendicitis the bowel transmissibility of the intestinal flora increases in relation to... more In acute appendicitis the bowel transmissibility of the intestinal flora increases in relation to inflammation and edema formation. We can therefore observe an immunologic response in patients, which is detectable using different bacteria isolated from the normal intestinal flora. Our aim was to measure this immunologic reaction and to detect the relationship between this response and histologic types of acute appendicitis. Sera from 47 cases, comprising 38 patients suffering from appendicitis and 9 healthy controls, were examined. The sera were taken shortly before appendectomy and 14 days after operation. The antigens were lipopolysaccharides (LPS) extracted from bacteria of normal intestinal flora: Escherichia coli O21, O22, O33, O61, O68, Bacteroides fragilis and an absolute rough mutant: Shigella sonnei Re 4350. Antibodies were detected by ELISA. We showed a direct relationship between severity of appendicitis and registered antibody titer. Both aerobic and anaerobic bacteria play a role in infection in appendicitis. According to our serologic results the synergy of B. fragilis with E. coli from normal flora is more important in the initiation of inflammation, but in the perforation process the role of E. coli seems more important compared to that of B. fragilis.
Acta Microbiologica et Immunologica Hungarica, 2011
Binding of fluorescein isothiocyanate-labeled concanavalin A to a series of molecular species of ... more Binding of fluorescein isothiocyanate-labeled concanavalin A to a series of molecular species of lipopolysaccharide (LPS), purified from pathogenic bacteria, was studied via agarose gel precipitation experiments and the results were compared with available structural data.
Diagnostic Microbiology and Infectious Disease, 2014
Hantaviruses, one of the causative agents of viral hemorrhagic fevers, represent a considerable h... more Hantaviruses, one of the causative agents of viral hemorrhagic fevers, represent a considerable healthcare threat. In Hungary, Dobrava-Belgrade virus (DOBV) and Puumala virus (PUUV) are the main circulating hantavirus species, responsible for the clinical picture known as hemorrhagic fever with renal syndrome, a disease that may be accompanied by acute kidney injury (AKI), requiring hospitalization with occasionally prolonged recovery phase. A total of 20 patient sera were collected over a 2-year period from persons hospitalized with AKI, displaying clinical signs and laboratory findings directly suggestive for hantavirus infection. Samples were tested using an immunoblot assay, based on complete viral nucleocapsid proteins to detect patients' IgM and IgG antibodies against DOBV and PUUV. In parallel, all specimens were also tested by 1-step real-time TaqMan reverse-transcriptase polymerase chain reaction to confirm infection and to determine the causative hantavirus genotype. We present here the first Hungarian clinical study spanning across 2 years and dedicated specifically to assess acute kidney injuries, in the context of hantavirus prevalence.