Mukadder Çalıkoğlu | Mersin University (original) (raw)

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Papers by Mukadder Çalıkoğlu

Clinical Chemistry and Laboratory Medicine, 2000

There is an increasing interest in the concept that oxidant/antioxidant imbalance plays a role in... more There is an increasing interest in the concept that oxidant/antioxidant imbalance plays a role in the pathogenesis of chronic obstructive pulmonary disease (COPD). However, most of the studies are concentrated on the local antioxidant/oxidant balance. In this study, we investigated the oxidant/antioxidant balance in systemic circulation of patients with COPD. Serum malonyldialdehyde (MDA), vitamin C and erythrocyte reduced glutathione (GSH) were determined in patients during acute exacerbation and during the stable phase of the disease, and compared with age- and sex-matched healthy controls. The levels of serum MDA, vitamin C and erythrocyte GSH were determined according to Yagi, Beutler and Bauer et al., respectively. Serum MDA levels were significantly higher in patients compared to controls, and during acute exacerbation compared to the stable phase. MDA levels in patients with acute exacerbation and in those in stable phase were also higher than in controls. We found significantly decreased levels of erythrocyte GSH and serum vitamin C in patients with acute exacerbation and stable COPD compared to controls. Although smoking caused an increase in oxidative stress in controls, the measured parameters were not affected by smoking in the patient group. In conclusion, there is a systemic oxidant/antioxidant imbalance in COPD, and this imbalance is probably independent of smoking.

Türkiye Fiziksel Tıp ve Rehabilitasyon Dergisi, 2010

ABSTRACT Objective: In this study, we investigated the effect of osteoporosis on pulmonary functi... more ABSTRACT Objective: In this study, we investigated the effect of osteoporosis on pulmonary function and respiratory muscle strength in patients with male osteoporosis with mild vertebral compression fracture, but without kyphosis or vertebral fracture. Materials and Methods: Thirty-four men with primary osteoporosis and 17 healthy men, matched for age and weight, were evaluated. Body mass index, tobacco smoking, and degree of vertebral compression were recorded, and values for forced vital capacity (FVC), forced expiratory volume in 1 second (FEV1), forced expiratory flow (FEF), maximum voluntary ventilation (MVV), maximum inspiratory pressure (MIP), and maximum expiratory pressure (MEP) were determined. Results: Although no significant differences in the results of pulmonary function tests between the patients and control groups were noted, the values for MIP (p=0.015) and MEP (p=0.008) were significantly lower in the osteoporotic subjects. No difference in respiratory function was noted in the subjects with compression fractures when compared to those without fractures. Conclusion: In conclusion, we thought that osteoporosis may affect respiratory function in male patients with osteoporosis, even without kyphosis. Turk J Phys Med Rehab 2010;56:49-51.

Obesity Research & Clinical Practice, 2011

It is known that obesity causes to impairment of pulmonary functions. This impairment worsens wit... more It is known that obesity causes to impairment of pulmonary functions. This impairment worsens with aging.

Cell Biochemistry and Function, 2004

Biochemical Genetics, 2006

Although smoking is regarded as the most important causal factor in chronic obstructive pulmonary... more Although smoking is regarded as the most important causal factor in chronic obstructive pulmonary disease (COPD), only 10-20% of smokers develop symptomatic COPD, which indicates the presence of genetic predisposing factors in its pathogenesis. This study investigates the association between gene polymorphysims of glutathione S-transferases (GSTs) and COPD. Blood samples were taken from 149 patients and 150 healthy controls. Polymorphisms of GSTT1, GSTM1, and GSTP1 were genotyped using Real-Time PCR. Multivariate logistic regression was used to calculate odds ratios (ORs) and 95% confidence intervals between specific genotypes and COPD. There was no difference in the frequencies of the genotypes of GSTM1 and GSTT1 between the groups, but the GSTP1 Ile/Ile genotype was significantly higher in the patients than in the controls (61.1% vs. 38%). GSTP1 Ile/Val and Val/Val genotypes were associated with a decreased risk of COPD when compared to the Ile/Ile genotype (2.12-fold and 4-fold, respectively). Thus we suggest that the Val allele of GSTP1 may have a protective effect for development of COPD. Furthermore, when we evaluated the association between GSTP1 genes and smoking status, smokers with the GSTP1 Ile allele had an increased risk for the development of COPD. Among the combinations of the genotypes, the combination of GSTM1, GSTT1 null, and GSTP1 Val/Val was associated with the maximal increased risk (12-fold) of COPD. Thus to explain the ethiopathogenesis of COPD, investigation of a single gene family is inadequate. Based on our results and the previous data, further studies should be focused on the GSTP1 gene and the interactions with other genes such as polymorphisms of N-acetyltransferases, GSTM1 and GSTT1, microsomal epoxide hydrolase, and allelic variants of cytochrome P450.

European Journal of Radiology, 2004

The purpose of this study was to classify the accessory fissures of the lung and to assess their ... more The purpose of this study was to classify the accessory fissures of the lung and to assess their frequency by using high-resolution CT. Methods and patients: HRCT scans of 115 patients were prospectively reviewed. 1 mm thin sections were obtained at 10 mm intervals with a scan time of 1.9 s. The fissure and its relationship to the segmental bronchovascular structures were then evaluated on transverse sections. Results: Forty-four accessory fissures were detected in 35 of 115 patients. The most common accessory fissure was the inferior accessory fissure (12%). The second most common accessory fissure was the left minor fissure (8%). The right superior accessory fissure (5%), the accessory fissure between the medial and lateral segments of the right middle lobe (5%), and the accessory fissure between the superior and inferior segments of the lingula (5%) were seen in equal frequencies. Also, intersegmental accessory fissures, namely the fissure between the anterobasal and laterobasal of both the right (1%) and the left (2%) lower lobes were detected. We found only one subsegmental accessory fissure. Discussion and conclusion: The inferior accessory fissure and the left minor fissure were the most common accessory fissures in our study.

Clinical Chemistry and Laboratory Medicine, 2000

There is an increasing interest in the concept that oxidant/antioxidant imbalance plays a role in... more There is an increasing interest in the concept that oxidant/antioxidant imbalance plays a role in the pathogenesis of chronic obstructive pulmonary disease (COPD). However, most of the studies are concentrated on the local antioxidant/oxidant balance. In this study, we investigated the oxidant/antioxidant balance in systemic circulation of patients with COPD. Serum malonyldialdehyde (MDA), vitamin C and erythrocyte reduced glutathione (GSH) were determined in patients during acute exacerbation and during the stable phase of the disease, and compared with age- and sex-matched healthy controls. The levels of serum MDA, vitamin C and erythrocyte GSH were determined according to Yagi, Beutler and Bauer et al., respectively. Serum MDA levels were significantly higher in patients compared to controls, and during acute exacerbation compared to the stable phase. MDA levels in patients with acute exacerbation and in those in stable phase were also higher than in controls. We found significantly decreased levels of erythrocyte GSH and serum vitamin C in patients with acute exacerbation and stable COPD compared to controls. Although smoking caused an increase in oxidative stress in controls, the measured parameters were not affected by smoking in the patient group. In conclusion, there is a systemic oxidant/antioxidant imbalance in COPD, and this imbalance is probably independent of smoking.

Türkiye Fiziksel Tıp ve Rehabilitasyon Dergisi, 2010

ABSTRACT Objective: In this study, we investigated the effect of osteoporosis on pulmonary functi... more ABSTRACT Objective: In this study, we investigated the effect of osteoporosis on pulmonary function and respiratory muscle strength in patients with male osteoporosis with mild vertebral compression fracture, but without kyphosis or vertebral fracture. Materials and Methods: Thirty-four men with primary osteoporosis and 17 healthy men, matched for age and weight, were evaluated. Body mass index, tobacco smoking, and degree of vertebral compression were recorded, and values for forced vital capacity (FVC), forced expiratory volume in 1 second (FEV1), forced expiratory flow (FEF), maximum voluntary ventilation (MVV), maximum inspiratory pressure (MIP), and maximum expiratory pressure (MEP) were determined. Results: Although no significant differences in the results of pulmonary function tests between the patients and control groups were noted, the values for MIP (p=0.015) and MEP (p=0.008) were significantly lower in the osteoporotic subjects. No difference in respiratory function was noted in the subjects with compression fractures when compared to those without fractures. Conclusion: In conclusion, we thought that osteoporosis may affect respiratory function in male patients with osteoporosis, even without kyphosis. Turk J Phys Med Rehab 2010;56:49-51.

Obesity Research & Clinical Practice, 2011

It is known that obesity causes to impairment of pulmonary functions. This impairment worsens wit... more It is known that obesity causes to impairment of pulmonary functions. This impairment worsens with aging.

Cell Biochemistry and Function, 2004

Biochemical Genetics, 2006

Although smoking is regarded as the most important causal factor in chronic obstructive pulmonary... more Although smoking is regarded as the most important causal factor in chronic obstructive pulmonary disease (COPD), only 10-20% of smokers develop symptomatic COPD, which indicates the presence of genetic predisposing factors in its pathogenesis. This study investigates the association between gene polymorphysims of glutathione S-transferases (GSTs) and COPD. Blood samples were taken from 149 patients and 150 healthy controls. Polymorphisms of GSTT1, GSTM1, and GSTP1 were genotyped using Real-Time PCR. Multivariate logistic regression was used to calculate odds ratios (ORs) and 95% confidence intervals between specific genotypes and COPD. There was no difference in the frequencies of the genotypes of GSTM1 and GSTT1 between the groups, but the GSTP1 Ile/Ile genotype was significantly higher in the patients than in the controls (61.1% vs. 38%). GSTP1 Ile/Val and Val/Val genotypes were associated with a decreased risk of COPD when compared to the Ile/Ile genotype (2.12-fold and 4-fold, respectively). Thus we suggest that the Val allele of GSTP1 may have a protective effect for development of COPD. Furthermore, when we evaluated the association between GSTP1 genes and smoking status, smokers with the GSTP1 Ile allele had an increased risk for the development of COPD. Among the combinations of the genotypes, the combination of GSTM1, GSTT1 null, and GSTP1 Val/Val was associated with the maximal increased risk (12-fold) of COPD. Thus to explain the ethiopathogenesis of COPD, investigation of a single gene family is inadequate. Based on our results and the previous data, further studies should be focused on the GSTP1 gene and the interactions with other genes such as polymorphisms of N-acetyltransferases, GSTM1 and GSTT1, microsomal epoxide hydrolase, and allelic variants of cytochrome P450.

European Journal of Radiology, 2004

The purpose of this study was to classify the accessory fissures of the lung and to assess their ... more The purpose of this study was to classify the accessory fissures of the lung and to assess their frequency by using high-resolution CT. Methods and patients: HRCT scans of 115 patients were prospectively reviewed. 1 mm thin sections were obtained at 10 mm intervals with a scan time of 1.9 s. The fissure and its relationship to the segmental bronchovascular structures were then evaluated on transverse sections. Results: Forty-four accessory fissures were detected in 35 of 115 patients. The most common accessory fissure was the inferior accessory fissure (12%). The second most common accessory fissure was the left minor fissure (8%). The right superior accessory fissure (5%), the accessory fissure between the medial and lateral segments of the right middle lobe (5%), and the accessory fissure between the superior and inferior segments of the lingula (5%) were seen in equal frequencies. Also, intersegmental accessory fissures, namely the fissure between the anterobasal and laterobasal of both the right (1%) and the left (2%) lower lobes were detected. We found only one subsegmental accessory fissure. Discussion and conclusion: The inferior accessory fissure and the left minor fissure were the most common accessory fissures in our study.