Vasile Cozma | University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca (original) (raw)

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Papers by Vasile Cozma

Research paper thumbnail of Screening for anthelmintic resistance in equid strongyles (Nematoda) in Romania

Folia Parasitologica, 2015

Research paper thumbnail of Clinical and Vaccine Immunology

Research paper thumbnail of Seroprevalence of Neospora caninum in dairy cattle from north-west and centre of Romania

Research paper thumbnail of Transstadial Transmission of Borrelia turcica in Hyalomma aegyptium Ticks

PloS one, 2015

Borrelia turcica comprises the third major group of arthropod-transmitted borreliae and is phylog... more Borrelia turcica comprises the third major group of arthropod-transmitted borreliae and is phylogenetically divergent from other Borrelia groups. The novel group of Borrelia was initially isolated from Hyalomma aegyptium ticks in Turkey and it was recently found in blood and multiple organs of tortoises exported from Jordan to Japan. However, the ecology of these spirochetes and their development in ticks or the vertebrate hosts were not investigated in detail; our aims were to isolate the pathogen and to evaluate the possibility of transstadial transmission of Borrelia turcica by H. aegyptium ticks. Ticks were collected from Testudo graeca tortoises during the summer of 2013 from southeastern Romania. Engorged nymphs were successfully molted to the adult stage. Alive B. turcica was isolated from molted ticks by using Barbour-Stoenner-Kelly (BSK) II medium. Four pure cultures of spirochetes were obtained and analyzed by PCR and sequencing. Sequence analysis of glpQ, gyrB and flaB re...

Research paper thumbnail of Characterisation of a high-frequency gene encoding a strongly antigenic cystatin-like protein from Trichinella spiralis at its early invasion stage

Parasites & vectors, Jan 5, 2015

BackgroundThe intestinal phase is the early invasion stage of Trichinella spiralis (T. spiralis),... more BackgroundThe intestinal phase is the early invasion stage of Trichinella spiralis (T. spiralis), in which muscle larvae invade intestine epithelial cells and then develop into adult worms to breed newborn larvae. Thus, intestinal infective larvae are first exposed to the immune system of the host, and antigens from the worms may be the earliest marker in the diagnosis of trichinellosis and may contribute to vaccine development to prevent Trichinella infections in pigs.MethodsA cDNA library of intestinal infective larvae of T. spiralis at 6 hours post infection (p.i.) was constructed and immunoscreened using serum collected from pigs that were infected with T. spiralis at 26 days p.i. T. spiralis cystatin-like protein (Ts-CLP) gene encoding a 45.9 kDa protein was cloned and expressed in Escherichia coli. The rabbit antisera were generated and used to determine the location of Ts-CLP in the parasite. Transcription levels of Ts-CLP in different developmental stages of T. spiralis were...

Research paper thumbnail of Elemente de diagnostic radiografic, ecografic şi laparoscopic în echinococcoza chistică experimentală la miei

Research paper thumbnail of Eficacitatea vaccinului EWEGUARD (American Cyanamid Company) în prevenirea anaerobiozelor şi în terapia unor helmintoze digestive şi pulmonare la ovine

Research paper thumbnail of Cercetări privind eficacitatea produsului Mondolar 10%(monensin) granulat (KRKA, Slovenia) în eimerioza experimentală a puilor de găină

Research paper thumbnail of Parasitic fauna in farmed trouts in Tismana, Romania

Research paper thumbnail of Studiu comparativ asupra evoluŃiei ciclului eimerian în celulele membranei corioalantoidiene şi intestinale

TIRIFON EUGENIA, ŞUTEU E.*, DULCEANU N.**, COZMA V.*, BABA AI*, TURCU D.***, TEUŞDEA V.****, MILI... more TIRIFON EUGENIA, ŞUTEU E.*, DULCEANU N.**, COZMA V.*, BABA AI*, TURCU D.***, TEUŞDEA V.****, MILITARU MANUELA*****, CĂTOI C.*, MIRCEAN VIORICA*, CERNEA CRISTINA*, GHERMAN C.*, ŞANDRU DANA*, FIł N.*, BĂDULESCU NICULINA****** ... Institutul NaŃional de ...

Research paper thumbnail of Utilizarea reacŃiei imunoenzimatice ELISA în diagnosticul eimeriozei aviare

Research paper thumbnail of Testarea eficacităŃii imunoprofilactice a vaccinului Nobillis COX ATM (INTERVET, Olanda) în eimerioza experimentală a puilor broiler

REZUMAT. Eimerioza produce pagube economice importante în sectorul avicol iar la acestea se adaug... more REZUMAT. Eimerioza produce pagube economice importante în sectorul avicol iar la acestea se adaug ă apariŃia de sue chimiorezistente la coccidiostaticele existente la ora actuală. Vaccinarea, la care se adug ă condiŃii de zooigien ă corespunz ătoare, s-a dovedit a fi singura metod ă practic ă, viabilă. Când puii sunt infecta Ńi cu un num ăr mic de oochisturi, imunitatea

Research paper thumbnail of Passive Immunity in Poultry Eimeriosis

Research paper thumbnail of Toxoplasmosis: aspects concerning the epidemiology and the paraimmune prophylaxis

Research paper thumbnail of Aspecte clinice în reinfecŃia experimentală cu M. canis la pisică

Research paper thumbnail of Controlul atacului cu Pulicidae la câine, prin utilizarea produselor Scalibor (colier)(Intervet), Taktic (Intervet), Neo-Toxan (A&S International 2000)

Research paper thumbnail of The efficacy of Monensin 20% in chimioprevention and therapy on poultry eimeriosis

Research paper thumbnail of 10. Comisia Medicină Veterinară

Research paper thumbnail of Aspecte clinice în infecŃia şi reinfecŃia exerimentală cu Microsporum canis la pisici

Research paper thumbnail of The incidence of endoparasitosis diagnosticated in slaughter house, on central Transilvania buffaloes

SUMMARY. Taking into consideration the big enough number of the slaughtered buffaloes and the evo... more SUMMARY. Taking into consideration the big enough number of the slaughtered buffaloes and the evolution of some important parasitosis at this species, some of them being zoonosis, we consider very important the research regarding the incidence of endoparasitosis at this species and their correlation with different determinating factors. For this, were examinated 134 buffaloes providing from 3 dictricts from Central

Research paper thumbnail of Screening for anthelmintic resistance in equid strongyles (Nematoda) in Romania

Folia Parasitologica, 2015

Research paper thumbnail of Clinical and Vaccine Immunology

Research paper thumbnail of Seroprevalence of Neospora caninum in dairy cattle from north-west and centre of Romania

Research paper thumbnail of Transstadial Transmission of Borrelia turcica in Hyalomma aegyptium Ticks

PloS one, 2015

Borrelia turcica comprises the third major group of arthropod-transmitted borreliae and is phylog... more Borrelia turcica comprises the third major group of arthropod-transmitted borreliae and is phylogenetically divergent from other Borrelia groups. The novel group of Borrelia was initially isolated from Hyalomma aegyptium ticks in Turkey and it was recently found in blood and multiple organs of tortoises exported from Jordan to Japan. However, the ecology of these spirochetes and their development in ticks or the vertebrate hosts were not investigated in detail; our aims were to isolate the pathogen and to evaluate the possibility of transstadial transmission of Borrelia turcica by H. aegyptium ticks. Ticks were collected from Testudo graeca tortoises during the summer of 2013 from southeastern Romania. Engorged nymphs were successfully molted to the adult stage. Alive B. turcica was isolated from molted ticks by using Barbour-Stoenner-Kelly (BSK) II medium. Four pure cultures of spirochetes were obtained and analyzed by PCR and sequencing. Sequence analysis of glpQ, gyrB and flaB re...

Research paper thumbnail of Characterisation of a high-frequency gene encoding a strongly antigenic cystatin-like protein from Trichinella spiralis at its early invasion stage

Parasites & vectors, Jan 5, 2015

BackgroundThe intestinal phase is the early invasion stage of Trichinella spiralis (T. spiralis),... more BackgroundThe intestinal phase is the early invasion stage of Trichinella spiralis (T. spiralis), in which muscle larvae invade intestine epithelial cells and then develop into adult worms to breed newborn larvae. Thus, intestinal infective larvae are first exposed to the immune system of the host, and antigens from the worms may be the earliest marker in the diagnosis of trichinellosis and may contribute to vaccine development to prevent Trichinella infections in pigs.MethodsA cDNA library of intestinal infective larvae of T. spiralis at 6 hours post infection (p.i.) was constructed and immunoscreened using serum collected from pigs that were infected with T. spiralis at 26 days p.i. T. spiralis cystatin-like protein (Ts-CLP) gene encoding a 45.9 kDa protein was cloned and expressed in Escherichia coli. The rabbit antisera were generated and used to determine the location of Ts-CLP in the parasite. Transcription levels of Ts-CLP in different developmental stages of T. spiralis were...

Research paper thumbnail of Elemente de diagnostic radiografic, ecografic şi laparoscopic în echinococcoza chistică experimentală la miei

Research paper thumbnail of Eficacitatea vaccinului EWEGUARD (American Cyanamid Company) în prevenirea anaerobiozelor şi în terapia unor helmintoze digestive şi pulmonare la ovine

Research paper thumbnail of Cercetări privind eficacitatea produsului Mondolar 10%(monensin) granulat (KRKA, Slovenia) în eimerioza experimentală a puilor de găină

Research paper thumbnail of Parasitic fauna in farmed trouts in Tismana, Romania

Research paper thumbnail of Studiu comparativ asupra evoluŃiei ciclului eimerian în celulele membranei corioalantoidiene şi intestinale

TIRIFON EUGENIA, ŞUTEU E.*, DULCEANU N.**, COZMA V.*, BABA AI*, TURCU D.***, TEUŞDEA V.****, MILI... more TIRIFON EUGENIA, ŞUTEU E.*, DULCEANU N.**, COZMA V.*, BABA AI*, TURCU D.***, TEUŞDEA V.****, MILITARU MANUELA*****, CĂTOI C.*, MIRCEAN VIORICA*, CERNEA CRISTINA*, GHERMAN C.*, ŞANDRU DANA*, FIł N.*, BĂDULESCU NICULINA****** ... Institutul NaŃional de ...

Research paper thumbnail of Utilizarea reacŃiei imunoenzimatice ELISA în diagnosticul eimeriozei aviare

Research paper thumbnail of Testarea eficacităŃii imunoprofilactice a vaccinului Nobillis COX ATM (INTERVET, Olanda) în eimerioza experimentală a puilor broiler

REZUMAT. Eimerioza produce pagube economice importante în sectorul avicol iar la acestea se adaug... more REZUMAT. Eimerioza produce pagube economice importante în sectorul avicol iar la acestea se adaug ă apariŃia de sue chimiorezistente la coccidiostaticele existente la ora actuală. Vaccinarea, la care se adug ă condiŃii de zooigien ă corespunz ătoare, s-a dovedit a fi singura metod ă practic ă, viabilă. Când puii sunt infecta Ńi cu un num ăr mic de oochisturi, imunitatea

Research paper thumbnail of Passive Immunity in Poultry Eimeriosis

Research paper thumbnail of Toxoplasmosis: aspects concerning the epidemiology and the paraimmune prophylaxis

Research paper thumbnail of Aspecte clinice în reinfecŃia experimentală cu M. canis la pisică

Research paper thumbnail of Controlul atacului cu Pulicidae la câine, prin utilizarea produselor Scalibor (colier)(Intervet), Taktic (Intervet), Neo-Toxan (A&S International 2000)

Research paper thumbnail of The efficacy of Monensin 20% in chimioprevention and therapy on poultry eimeriosis

Research paper thumbnail of 10. Comisia Medicină Veterinară

Research paper thumbnail of Aspecte clinice în infecŃia şi reinfecŃia exerimentală cu Microsporum canis la pisici

Research paper thumbnail of The incidence of endoparasitosis diagnosticated in slaughter house, on central Transilvania buffaloes

SUMMARY. Taking into consideration the big enough number of the slaughtered buffaloes and the evo... more SUMMARY. Taking into consideration the big enough number of the slaughtered buffaloes and the evolution of some important parasitosis at this species, some of them being zoonosis, we consider very important the research regarding the incidence of endoparasitosis at this species and their correlation with different determinating factors. For this, were examinated 134 buffaloes providing from 3 dictricts from Central

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