Jaya Arora | M S University (original) (raw)
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The Great Indian Desert known as the Thar Desert occupies about 60% of the area of Rajasthan-the ... more The Great Indian Desert known as the Thar Desert occupies about 60% of the area of Rajasthan-the largest state of India. It is one of the most heavily populated (in terms of both people and cattle) deserts of the world. The animal and human populations exert tremendous pressure on the scant vegetation of the region, making several plants vulnerable to becoming endangered. Inherent biological problems associated with these plants make their survival difficult and have forced adaptation to the harsh environment. The biological activities of these plants range from analgesic, antifungal, antimicrobial, hypolipidemic to hepatoprotective and anticancerous. This chapter reviews the biological problems faced by the medicinal plants of this region, their bioactive molecules, as well as biotechnological approaches aimed at improving and conserving these plants.
in Vitro Cellular & Developmental Biology-plant, 2010
This report demonstrates the elicitation effect on growth and stilbene accumulation in cell cultu... more This report demonstrates the elicitation effect on growth and stilbene accumulation in cell cultures of Cayratia trifolia (Vitaceae) by an extract of the angiosperm parasite Cuscuta reflexa and salicylic acid in combination with sucrose feeding. Cell cultures of C. trifolia, a tropical liana, were maintained in liquid Murashige and Skoog's basal medium containing 0.25 mg l−1 naphthalene acetic acid, 0.2 mg l−1 kinetin with
Acta Physiologiae Plantarum, 2009
The Root cultures of Cayratia trifolia (Vitaceae) a tropical lianas, were maintained in liquid Mu... more The Root cultures of Cayratia trifolia (Vitaceae) a tropical lianas, were maintained in liquid Murashige and Skoog's medium containing 0.5 mg l -1 NAA, 0.1 mg l -1 kinetin with 3% sucrose. These root cultures when grown with 6% sucrose accumulated stilbenes (piceid, resveratrol, viniferin, ampelopsin) in high amounts, which on elicitation by 500 mg l -1 yeast extract, 50 lM salicylic acid (SA), 50 lM methyl jasmonate (MeJa), 500 lM ethrel added at 25th day, increased up to ninefolds (7.1 mg l -1 ). Addition of alar or phenylalanine along with the elicitors further enhanced the stilbenes content. In the present study, stilbenes accumulation up to 12 folds (9.2 mg l -1 ) was obtained with SA and alar. The SA was the most effective in increasing the stilbenes contents while less than control values were recorded in the cells treated with MeJa. The roots could be grown up to 2 l flasks. The present work demonstrates that presence of precursor and sucrose during elicitation at an appropriate time combined with growth retardation significantly increased the production of stilbenes in C. trifolia cell cultures.
The Great Indian Desert known as the Thar Desert occupies about 60% of the area of Rajasthan-the ... more The Great Indian Desert known as the Thar Desert occupies about 60% of the area of Rajasthan-the largest state of India. It is one of the most heavily populated (in terms of both people and cattle) deserts of the world. The animal and human populations exert tremendous pressure on the scant vegetation of the region, making several plants vulnerable to becoming endangered. Inherent biological problems associated with these plants make their survival difficult and have forced adaptation to the harsh environment. The biological activities of these plants range from analgesic, antifungal, antimicrobial, hypolipidemic to hepatoprotective and anticancerous. This chapter reviews the biological problems faced by the medicinal plants of this region, their bioactive molecules, as well as biotechnological approaches aimed at improving and conserving these plants.
in Vitro Cellular & Developmental Biology-plant, 2010
This report demonstrates the elicitation effect on growth and stilbene accumulation in cell cultu... more This report demonstrates the elicitation effect on growth and stilbene accumulation in cell cultures of Cayratia trifolia (Vitaceae) by an extract of the angiosperm parasite Cuscuta reflexa and salicylic acid in combination with sucrose feeding. Cell cultures of C. trifolia, a tropical liana, were maintained in liquid Murashige and Skoog's basal medium containing 0.25 mg l−1 naphthalene acetic acid, 0.2 mg l−1 kinetin with
Acta Physiologiae Plantarum, 2009
The Root cultures of Cayratia trifolia (Vitaceae) a tropical lianas, were maintained in liquid Mu... more The Root cultures of Cayratia trifolia (Vitaceae) a tropical lianas, were maintained in liquid Murashige and Skoog's medium containing 0.5 mg l -1 NAA, 0.1 mg l -1 kinetin with 3% sucrose. These root cultures when grown with 6% sucrose accumulated stilbenes (piceid, resveratrol, viniferin, ampelopsin) in high amounts, which on elicitation by 500 mg l -1 yeast extract, 50 lM salicylic acid (SA), 50 lM methyl jasmonate (MeJa), 500 lM ethrel added at 25th day, increased up to ninefolds (7.1 mg l -1 ). Addition of alar or phenylalanine along with the elicitors further enhanced the stilbenes content. In the present study, stilbenes accumulation up to 12 folds (9.2 mg l -1 ) was obtained with SA and alar. The SA was the most effective in increasing the stilbenes contents while less than control values were recorded in the cells treated with MeJa. The roots could be grown up to 2 l flasks. The present work demonstrates that presence of precursor and sucrose during elicitation at an appropriate time combined with growth retardation significantly increased the production of stilbenes in C. trifolia cell cultures.