Samira jafarzadeh holagh | Tarbiat Modares University (original) (raw)
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Papers by Samira jafarzadeh holagh
Journal of Colloid and Interface Science, 2018
Nanocarrier-based drug delivery systems have been explored extensively in cancer therapy. Among t... more Nanocarrier-based drug delivery systems have been explored extensively in cancer therapy. Among the vast number of different nanocarrier systems applied to deliver chemotherapeutics to cancer tumor, intelligent systems which deliver drug at various sites in the body have attracted considerable attentions. Finding a specific stimulant that triggers the carrier to release its payload in the target tissue is a key parameter for efficacy of delivery systems. Acidic pH of cancer tumor helps a pH-sensitive carrier to release drug at the tumor site. In this study, a pH-sensitive mixed micellar system was developed using Dextran-Stearic Acid (Dex-SA) and Dextran-Histidine (Dex-His) conjugated polymers to deliver doxorubicin (DOX) to cancer cells. Drug release from this micellar system showed higher release rate at acidic pH than that of in neutral environment, where the release was 56 and 76% at pH 7.4 and acidic pH, respectively. Finally, the in vitro cytotoxicity and cell uptake of DOX-loaded micelles and free DOX on U87 MG cell line showed that micellar systems had more anti-proliferation effect and uptake compared to free drug.
Journal of Drug Delivery Science and Technology, 2021
Abstract Over the past decades, natural polysaccharides have received considerable attention to d... more Abstract Over the past decades, natural polysaccharides have received considerable attention to developing nano-drug delivery systems for the delivery of anticancer drugs. However, the low solubility of these agents within the biological fluid has reduced their efficacy in inhibiting the growth of cancer cells. In this study, self-assembled cationic polysaccharides based on dextran-stearic acid-spermine conjugates (D-SASp) were synthesized, and their potential as carriers for sustained release of rapamycin was demonstrated. Nanomicelles (smaller than 200 nm) with relatively narrow size distribution were obtained through dialysis of D-SASp solution in DMSO against distilled water. The sustained release of rapamycin from nanocarriers occurred without initial burst release. The in vitro toxicity studies with the U87 MG cell line confirmed that synthesized polymers were not toxic up to 0.4 mg/mL, and cytotoxicity of rapamycin-loaded micelles was higher than that of free rapamycin. Furthermore, flow cytometry analysis fully supported the time-dependent cellular uptake of the nanocarriers. In addition, the presence of conjugated spermine with a positive charge resulted in increased transport of the nanomicelles into the cells.
Journal of Colloid and Interface Science, 2018
Nanocarrier-based drug delivery systems have been explored extensively in cancer therapy. Among t... more Nanocarrier-based drug delivery systems have been explored extensively in cancer therapy. Among the vast number of different nanocarrier systems applied to deliver chemotherapeutics to cancer tumor, intelligent systems which deliver drug at various sites in the body have attracted considerable attentions. Finding a specific stimulant that triggers the carrier to release its payload in the target tissue is a key parameter for efficacy of delivery systems. Acidic pH of cancer tumor helps a pH-sensitive carrier to release drug at the tumor site. In this study, a pH-sensitive mixed micellar system was developed using Dextran-Stearic Acid (Dex-SA) and Dextran-Histidine (Dex-His) conjugated polymers to deliver doxorubicin (DOX) to cancer cells. Drug release from this micellar system showed higher release rate at acidic pH than that of in neutral environment, where the release was 56 and 76% at pH 7.4 and acidic pH, respectively. Finally, the in vitro cytotoxicity and cell uptake of DOX-loaded micelles and free DOX on U87 MG cell line showed that micellar systems had more anti-proliferation effect and uptake compared to free drug.
Journal of Drug Delivery Science and Technology, 2021
Abstract Over the past decades, natural polysaccharides have received considerable attention to d... more Abstract Over the past decades, natural polysaccharides have received considerable attention to developing nano-drug delivery systems for the delivery of anticancer drugs. However, the low solubility of these agents within the biological fluid has reduced their efficacy in inhibiting the growth of cancer cells. In this study, self-assembled cationic polysaccharides based on dextran-stearic acid-spermine conjugates (D-SASp) were synthesized, and their potential as carriers for sustained release of rapamycin was demonstrated. Nanomicelles (smaller than 200 nm) with relatively narrow size distribution were obtained through dialysis of D-SASp solution in DMSO against distilled water. The sustained release of rapamycin from nanocarriers occurred without initial burst release. The in vitro toxicity studies with the U87 MG cell line confirmed that synthesized polymers were not toxic up to 0.4 mg/mL, and cytotoxicity of rapamycin-loaded micelles was higher than that of free rapamycin. Furthermore, flow cytometry analysis fully supported the time-dependent cellular uptake of the nanocarriers. In addition, the presence of conjugated spermine with a positive charge resulted in increased transport of the nanomicelles into the cells.