Nidhal A. A Mohammed - AL-Nahrain university (original) (raw)

Papers by Nidhal A. A Mohammed

Circulating-Peripheral Blood Naturally Occurring CD4+ CD25+ Regulatory T Cells and CD4+ T Cells in Chronic Rheumatic Heart Disease

IRAQI JOURNAL OF …, 2000

Background The development of autoimmune disease involves a breakdown in the mechanisms that cont... more Background The development of autoimmune disease involves a breakdown in the mechanisms that control T cell tolerance to self antigens, these mechanisms are many and complex, and they integrate as immunoregulation. Among the cells that might be ...

Background: Hodgkin's lymphoma (HL) and Non-Hodgkin's lymphoma(NHL) are characterized by a profou... more Background: Hodgkin's lymphoma (HL) and Non-Hodgkin's lymphoma(NHL) are characterized by a profound disturbance of the cell cycle and apoptosis regulation., Ki-67 and P53 expression has been shown to be associated with adverse clinical outcome in a variety of malignant hematological disorders, including HL and NHL. Aim: This study aimed to clarify Ki-67 expression level and its correlation with p53 expression in HL and NHL. Material and methods: total of 28 newly diagnosed HL and NHL patients were investigated for Ki-67 expression and its correlation to p53 expression by immunohistochemical technique. Results: Ki-67 expression was observed in 46.7 % of HL patients while 7.7% of NHL patients gave positive Ki-67 expression. A significant Association between thetype of lymphoma and Ki67 expression (p<0.05) noticed. The histopathologcal type appeared to be 57.1% mixed cellularity and 37.5% of them showed positive Ki67 expression , while 42.9 % were intermediate grade and only 16.7 % showed positive Ki67 expression. There were no significant correlation between the histopathologcal type and Ki67 expression. only 4 (14.3 %) of the cases were p53 negative ,while the rest 24 cases were p53 positive ,and the majority (66.7%) were within score 2. Eight cases (28.6 %) were positive for Ki-67 and p53 , Ki-67 was negative in 71.4% cases , and four of them were negative for p53 and the rest 16 (80%)cases were p53 positive .A significant correlation was found between p53 and Ki-67 expression where(r=0.608,p=0.001). conclusion : it is suggested that immunohistochemical studies of p53 and Ki-67 expression in tumor tissue including HL and NHL can help in monitoring of patients at risk, and the markers may aid in controlling the progression of lymphoma and detect the degree of aggressiveness of the disease to give suitable treatment and management of patients .

Detection of IL-10, IFN-γ and IL-8 in sera of patients with recurrent spontaneous abortion

Background: Th1-type cytokines secretion such as IFN-γ, and Th2 cytokines such as IL10, have been... more Background: Th1-type cytokines secretion such as IFN-γ, and Th2 cytokines such as IL10, have been shown to exert deleterious effects on pregnancy, inhibiting fetal growth and development Objective: Estimation of Interleukin-10 (IL10), IL-8 and IFN-γ levels in sera of patients with recurrent spontaneous abortion (RSA) using ELISA method. Method: A total of one hundred and nineteen women, ranged from the mean age (23.9 – 28.5)years, were enrolled in the current study and were further classified into three categories: Group A- Recurrent spontaneous abortion (RSA): n= 62 women, with a mean age of (28.5 + 0.68);Group B- non- recurrent spontaneous abortion (non-RSA): n= 34 women, with a mean age of (26.4 ± 0.85)and group C- Control (successful pregnancy): n= 23 women, with a mean age of (23.9 ± 0.88). From each patient and control blood sample was collected and serum was 58eparated. Estimation of Interleukin-10 (IL-10), IL-8 IFN-γ levels in sera of patients was done using ELISA method. Re...

Zanco Journal of Medical Sciences, 2013

Background and objective: Prevention of bacterial adhesion is an attractive target for the develo... more Background and objective: Prevention of bacterial adhesion is an attractive target for the development of new therapies in the prevention of bacterial infection. The aim of this study is to investigate the effects of pomegranate peel extract, vitamin C, combination of pomegranate peel extract and vitamin C & gemifloxacin on adhesion of E.coli to uroepithelial cells. Methods: Uroepithelial cells were incubated with E. coli ATCC 25922 bacteria previously exposed to either the aqueous extract of pomegranate peel (AEPP), vitamin C, combination of both or gemifloxacin and the adherence was assessed by light microscopy. Results: AEPP showed good antibacterial activity with minimum inhibitory concentration MIC of 25μg/ml and 10 mmol/ml for vitamin C and upon combination, the MIC was10µg/ml &5mmol/ml for AEPP & vitamin C respectively whereas for gemifloxacin was 0.03μg/ml. In vitro and in vivo adhesion of E.coli to uroepithelial cells were significantly inhibited by AEPP, vitamin C alone and in combination and by gemifloxacin. Conclusion: AEPP showed good antibacterial activity, inhibited E.coli adhesion to uroepithelial cells and were potentiated by vitamin C.

Chlamydia Trachomatis and Recurrent Spontaneous Abortion in Iraqi Pregnant Women

Iraqi journal of medical sciences, 2012

Background Certain infectious agents have been identified more frequently in cultures from women ... more Background Certain infectious agents have been identified more frequently in cultures from women who have had a spontaneous pregnancy loss; these include Ureaplasma urealyticum, Mycoplasma hominis, and Chlamydia. Objective The aim of the study was to evaluate the frequency of Chlamydia trachomatis infection among women who experienced recurrent spontaneous abortion. Methods A total of 119 women, age ranged from 23.9−28.5 years were enrolled in the current study and were classified into: Group ARecurrent spontaneous abortion (RSA): n= 62 women, with a mean age of (28.5±0.68); Group Bnonrecurrent spontaneous abortion (non-RSA): n= 34 women, with a mean age of (26.4±0.85) and group CControl (successful pregnancy): n= 23 women, with a mean age of (23.9±0.88). From each patient and control blood and urine samples were collected. Urinalysis test strips including Leukocytes esterase in urine was done, and estimation of IgM levels against Chlamydia trachomatis in sera of patients was done u...

Serum Immunoglobulin G, M and a Levels in Children with Nephrotic Syndrome and Its Correlation with Biochemical Parameters

Pakistan Journal of Biotechnology, Jun 25, 2018

Expression of Ki 67 and p 53 Proteins in Hodgkin ׳ s Lymphomas and Non Hodgkin ׳ s Lymphomapatients using immunohistochemistry

Background: Hodgkin’s lymphoma (HL) and Non-Hodgkin’s lymphoma( NHL) are characterized by a profo... more Background: Hodgkin’s lymphoma (HL) and Non-Hodgkin’s lymphoma( NHL) are characterized by a profound disturbance of the cell cycle and apoptosis regulation., Ki-67 and P53 expression has been shown to be associated with adverse clinical outcome in a variety of malignant hematological disorders, including HL and NHL. Aim: This study aimed to clarify Ki-67 expression level and its correlation with p53 expression in HL and NHL. Material and methods: total of 28 newly diagnosed HL and NHL patients were investigated for Ki-67 expression and its correlation to p53 expression by immunohistochemical technique. Results: Ki-67 expression was observed in 46.7 % of HL patients while 7.7% of NHL patients gave positive Ki-67 expression. A significant Association between thetype of lymphoma and Ki67 expression (p<0.05) noticed. The histopathologcal type appeared to be 57.1% mixed cellularity and 37.5% of them showed positive Ki67 expression , while 42.9 % were intermediate grade and only 16.7 %...

It is becoming increasingly clear that ATG16L1 T300Aasan interacting autophagy pathway, as many o... more It is becoming increasingly clear that ATG16L1 T300Aasan interacting autophagy pathway, as many of the genes encoded by these risk loci mediate common cellular functions, such as microbial recognition, autophagy, cytokine signaling, epithelial barrier maintenance, lymphocyte activation, metabolism, and endoplasmic reticulum stress responses(Cho and Brant 2011, Graham and Xavier 2013). ATG16L1 encodes a key autophagy protein, which functions as part of a complex with autophagy proteins Atg5 and Atg12, and is responsible for the proper subcellular localization of the autophagy machinery(Kuma et al. 2002, Fujita et al. 2008). In 2007, genome-wide association scans (GWAS) identified a single nucleotide polymorphism (SNP) in the coding region of the ATG16L1 gene associated with an ISSN: 2319-7706 Volume 3 Number 12 (2014) pp. 633-642 http://www.ijcmas.com

Helicobacter pylori: The association between CagA positivity and p53 expression

H. pylori had been recognized as the causative agent of several gastric diseases and possessed wi... more H. pylori had been recognized as the causative agent of several gastric diseases and possessed wide variety of virulence factors, one of most important factors is CagA. p53 have central role in many cellular activities, one of them is the apoptosis. This study investigated the expression of p53 in the gastric epithelial cells in patients with Helicobacter pylori gastric illness in association with CagA positivity. Paraffin embedded tissue had been made form biopsies taken from 30 patients undergo O.G.D. and selected according to exclusion criteria. In addition, 10 apparently healthy volunteers were included as a control group. Procedures of ISH and IHC were used to detect CagA and p53 respectively. The CagA cytotoxin was detected in 21(70.0%) patients out of 30 and 9 (30.0%) gave negative results, p53 immunostaining shows 26/30 cases strong staining and 4/30 cases gave a moderate staining. The CagA positive H. pylori strains causes a higher p53 expression than the CagA negative stra...

DETECTION OF IL-8, IL-10 AND IFN- Γ mRNA IN TROPHOBLAST TISSUES OF RECURRENT SPONTANEOUS ABORTION USING IN SITU HYBERDIZATION

Iraqi journal of medical sciences, 2008

Background: Th1-type cytokines secretion such as IFN-γ, and Th2 cytokines such as IL10, have been... more Background: Th1-type cytokines secretion such as IFN-γ, and Th2 cytokines such as IL10, have been shown to exert deleterious effects on pregnancy, inhibiting fetal growth and development . Aim: Measurement of the locally concentrations of selected Th1 and Th2 cytokines in women with a history of recurrent spontaneous abortion (RSA) at the time of abortion using in situ hyperdization technique. Methods: A total of one hundred and nineteen women, ranged from the mean age (23.9 − 28.5)years, were enrolled in the current study and were further classified into three categories: Group ARecurrent spontaneous abortion (RSA): n= 62 women, with a mean age of (28.5 + 0.68);Group Bnonrecurrent spontaneous abortion (non-RSA): n= 34 women, with a mean age of (26.4 ± 0.85)and group CControl (successful pregnancy): n= 23 women, with a mean age of (23.9 ± 0.88). From each patient and control, placental tissues were collected. Trophoblasts tissues (an image for the local microenvironment) were screen...

JOURNAL OF EDUCATION AND SCIENCE, 2019

A ccurate and simple spectrophotometric method has been suggested for the determination of oxymet... more A ccurate and simple spectrophotometric method has been suggested for the determination of oxymetazoline hydrochloride (OMCl) in aqueous solution is developed. The present process included coupling of OMCl with diazotized sulphanilic acid reagent in the presence of sodium hydroxide. The orange colored azo dye formed very stable and soluble in water gives maximum absorption at 496 nm. The lineratey is obeyed over the range 20-400 g / 25 ml (0.8-16µg.ml-1) the molar absorptivity is equate to 2.30  10 4 l.mol-1 cm-1. The proposed method has been used to the determination of OMCl in two formulations with satisfactories results.

HLA-CLASS II RISK ALLELES CONTROL T-LYMPHOCYTE PROLIFERATION IN RESPONSE TO ENTEROVIRUS AND ADENOVIRUS ANTIGENS AND IgG ANTIBODY PREVALENCE IN NEWLY DIAGNOSED T1DM CHILDREN

Iraqi journal of medical sciences, 2007

Background: Viral infections are implicated in the pathogenesis of type 1 diabetes mellitus (T1DM... more Background: Viral infections are implicated in the pathogenesis of type 1 diabetes mellitus (T1DM) in a number of studies, and playing a role in the initiation of beta-cell damaging process.

Diyala University, 2010

Background: H. pylori had been recognized as the causative agent of several gastric diseases and ... more Background: H. pylori had been recognized as the causative agent of several gastric diseases and possessed wide variety of virulence factors, one of most important factors is CagA. Objective: This study investigated the expression of CagA cytotoxin on the gastric epithelial cells in patients with Helicobacter pylori gastric illness by using In Situ Hybridization technique. Methods: Paraffin embedded ssue had been made form biopsies taken from 30 pa ents undergo O.G.D. and selected according to exclusion criteria. In addi on 10 apparently healthy volunteers were included as a control group. Results: The CagA cytotoxin was detected in 21(70.0%) pa ents out of 30 and 9 (30.0%) gave negative results, on other hand control group showed no positive results. Conclusions: The CagA positive H. pylori strains cause the majority of H. pylori related gastric illness.

Induction of ICAM-1 and ICAM-3 in Women with Recurrent Pregnancy Loss

Iraqi journal of medical sciences, 2008

Background: Recurrent pregnancy loss (RPL) has been found to be associated with increase in the p... more Background: Recurrent pregnancy loss (RPL) has been found to be associated with increase in the pro-inflammatory cytokines which cause up-regulation of inflammatory mediators including cell adhesion molecules (CAMs) that might act in aggravation of this pathological process. Objective: To find out whether there is a relation between the pathology of RPL and the expression of intracellular adhesion molecule-1 (ICAM-1) and ICAM-3 at the feto-maternal interface in these patients. Methods: Immunohistochemistry technique was performed to detect and determine the expression of ICAM-1 and ICAM-3 using paraffin embedded sections of curate samples obtained from 40 women, who where divided into three groups: 24 women with RPL, 10 women with abortion for the first time, and 6 women with induced abortion.

Journal of the Faculty of Medicine Baghdad, 2014

Background:-M. pneumoniae is an important human pathogen that produces community-acquired respira... more Background:-M. pneumoniae is an important human pathogen that produces community-acquired respiratory tract infection. Diagnosis of M. pneumoniae infection is challenging and crucial for the timely initiation of the effective antibiotic therapy. Objective: This study has been undertaken to detect M. pneumoniae in respiratory samples (throat swabs, throat wash and sputum) in patients with respiratory tract infection qualitatively by conventional polymerase chain reaction (PCR). Also, more advanced one, real time PCR was used to determine mycoplasmal target gene qualitatively and quantitatively. Patients and methods: The study was performed on Seventy-five patients and thirty healthy subject as control. Genomic DNA was extracted and M. pneumoniae target gene (lipoprotein gene) was amplified using conventional PCR. Negative, positive controls and internal controls were involved in each experimental run. The amplified products were analyzed in 2% agarose gel and visualized using Red saf...

Expression of Galectin-9 and its association with clinico-pathological aspects and molecular subtypes of breast cancer

Gal-9 is a member of S-type lectins family that exhibit important functions in regulating immune ... more Gal-9 is a member of S-type lectins family that exhibit important functions in regulating immune cells. Many types of cells express Gal-9, moreover have variety of biological role. The present study was carried out to evaluate the immuno-expression of Gal-9 in two micro-environments, intra-tumoral and stromal, in breast cancer patients. The findings were correlated with the molecular subtypes and clinical groups. Sixty-one cases as patients group and seven have been selected as normal group for the study. Immunohistochemistry was used to evaluate the expression of ER, PR, Her2\\neu and Gal- 9. ER, PR and Her2\\neu were used for the molecular subtyping. Gal-9 assessed by H-SCORE system in two locations (intratumoral and stromal). Expression of Gal-9 shows high significant differences between the intratumoral and stromal expression scores (p ≤ 0.001) and shows no significant differences among molecular and clinical group. These findings may consider rational if we knew that Gal-9 exerting many functions.

Using In SituHybridization: Association between CagA Positivity and Interleukin-8 in Helicobacter pylori Gastritis

Background: H. pylori possessed wide variety of virulence factors, one of most important factors ... more Background: H. pylori possessed wide variety of virulence factors, one of most important factors is CagA. Interleukin-8 is proinflammatory cytokine plays as major human PMN chemo-attractant. Objective: This study investigated the association between expressions of CagA cytotoxin and interleukin-8 on the gastric epithelial cells in patients with Helicobacter pylori gastric illness by using In Situ Hybridization technique. Methods: Paraffin embedded tissue had been made form biopsies taken from 30 patients undergo O.G.D. and selected according to exclusion criteria. In addition 10 apparently healthy volunteers were included as a control group. Results: Significant positive linear relationship (Pearson correlation Coefficient=0.452, p=0. 012) was found between CagA positivity and interleukin-8 expression. Conclusions: This study conclude that infection with CagA positive H. pylori strains led to high levels of interleukin-8 production from the gastric epithelium.

Background: A number of studies have demonstrated that chlamydia trachomatis plays a prominent ro... more Background: A number of studies have demonstrated that chlamydia trachomatis plays a prominent role in disorders of the human reproductive system. Objective: This study was carried out to determine antibody levels of Chlamydia trachomatis among mothers with either normal deliveries or had cesarean section and their newborn babies, and the effect of various epidemiological, obstetric, and medical factors on antibody levels among the studied groups. Method: Serum specimens from 166 women with normal deliveries and their babies (group one) and 32 women with cesarean section and their babies (group two), were screened for C. Trachomatis antibodies by Micro ELISA method. Result: C. Trachomatis infection rate was 24% and 20.5% among women and babies in-group one,

CD14 and Bladder Cancer: Is There Any Correlation?

Iraqi journal of medical sciences, 2010

Background: Epithelial cells have evolved a variety of cell-and tissue-specific mechanisms for ba... more Background: Epithelial cells have evolved a variety of cell-and tissue-specific mechanisms for bacterial detection to enable cells to modulate the inflammatory response depending on the particular situation in a specific organ. These mechanisms provide a means of maintaining a proper balance between defense, tissue injury and their combined effects on organ function, and the moleculeCD14may have a role to play. Objective: to find any correlation between CD14 marker expression and bladder cancer. Material and methods: The immunoexpression of CD14 in paraffin sections from 96bladder cancer tissues and 36-bladder tissues from patients with other bladder disease rather than cancer was investigated using immunohistochemical assay (IHC). The patients were divided into three groups: Group-1: Newly diagnosed bladder cancer patients, 69(43.9%), Group-2:Post-chemotherapy patients, 27(17.2%), Group-3: Other bladder disorders rather than bladder cancer 36(22.9%). The final diagnosis of patients...

POSSIBLE ROLE OF Th-2 CELL-RELATED CYTOKINES (IL-6 AND IL-10) IN BREAST CANCER

Iraqi journal of medical sciences, 2010

Background: Breast cancer is a complex disease, many etiological agents are proposed to play a ro... more Background: Breast cancer is a complex disease, many etiological agents are proposed to play a role in its pathogenecity, one of these factors is cytokines. Objectives:In the present study we measured the concentration of IL-6 and IL-10 in serum of breast cancer patients and examined their association with clinicopathological variables including stages of the disease and estrogen/progesterone receptor (ER, PR) expression on tumor cells, to determine whether it associate with the disease progression. Subjects and Methods: The study included 80 subjects, it comprised of 45 Breast cancer patients, 12 patients with benign breast lesions and 23 apparently healthy controls. ELISA method has been used for estimation the level of IL-6 and IL-10 in serum of three studied groups. Results: There was an elevation of IL-6 and IL-10 level in the sera of BC patients with significant differences between BC and controls (p<0.001), also, this elevation was associated with progression of the tumor....

Circulating-Peripheral Blood Naturally Occurring CD4+ CD25+ Regulatory T Cells and CD4+ T Cells in Chronic Rheumatic Heart Disease

IRAQI JOURNAL OF …, 2000

Detection of IL-10, IFN-γ and IL-8 in sera of patients with recurrent spontaneous abortion

Zanco Journal of Medical Sciences, 2013

Chlamydia Trachomatis and Recurrent Spontaneous Abortion in Iraqi Pregnant Women

Iraqi journal of medical sciences, 2012

Serum Immunoglobulin G, M and a Levels in Children with Nephrotic Syndrome and Its Correlation with Biochemical Parameters

Pakistan Journal of Biotechnology, Jun 25, 2018

Expression of Ki 67 and p 53 Proteins in Hodgkin ׳ s Lymphomas and Non Hodgkin ׳ s Lymphomapatients using immunohistochemistry

Helicobacter pylori: The association between CagA positivity and p53 expression

DETECTION OF IL-8, IL-10 AND IFN- Γ mRNA IN TROPHOBLAST TISSUES OF RECURRENT SPONTANEOUS ABORTION USING IN SITU HYBERDIZATION

Iraqi journal of medical sciences, 2008

Background: Th1-type cytokines secretion such as IFN-γ, and Th2 cytokines such as IL10, have been... more Background: Th1-type cytokines secretion such as IFN-γ, and Th2 cytokines such as IL10, have been shown to exert deleterious effects on pregnancy, inhibiting fetal growth and development . Aim: Measurement of the locally concentrations of selected Th1 and Th2 cytokines in women with a history of recurrent spontaneous abortion (RSA) at the time of abortion using in situ hyperdization technique. Methods: A total of one hundred and nineteen women, ranged from the mean age (23.9 − 28.5)years, were enrolled in the current study and were further classified into three categories: Group ARecurrent spontaneous abortion (RSA): n= 62 women, with a mean age of (28.5 + 0.68);Group Bnonrecurrent spontaneous abortion (non-RSA): n= 34 women, with a mean age of (26.4 ± 0.85)and group CControl (successful pregnancy): n= 23 women, with a mean age of (23.9 ± 0.88). From each patient and control, placental tissues were collected. Trophoblasts tissues (an image for the local microenvironment) were screen...

JOURNAL OF EDUCATION AND SCIENCE, 2019

HLA-CLASS II RISK ALLELES CONTROL T-LYMPHOCYTE PROLIFERATION IN RESPONSE TO ENTEROVIRUS AND ADENOVIRUS ANTIGENS AND IgG ANTIBODY PREVALENCE IN NEWLY DIAGNOSED T1DM CHILDREN

Iraqi journal of medical sciences, 2007

Diyala University, 2010

Induction of ICAM-1 and ICAM-3 in Women with Recurrent Pregnancy Loss

Iraqi journal of medical sciences, 2008

Journal of the Faculty of Medicine Baghdad, 2014

Expression of Galectin-9 and its association with clinico-pathological aspects and molecular subtypes of breast cancer

Using In SituHybridization: Association between CagA Positivity and Interleukin-8 in Helicobacter pylori Gastritis

CD14 and Bladder Cancer: Is There Any Correlation?

Iraqi journal of medical sciences, 2010

POSSIBLE ROLE OF Th-2 CELL-RELATED CYTOKINES (IL-6 AND IL-10) IN BREAST CANCER

Iraqi journal of medical sciences, 2010

Background: Th1-type cytokines secretion such as IFN-γ, and Th2 cytokines such as IL-10, have bee... more Background: Th1-type cytokines secretion such as IFN-γ, and Th2 cytokines such as IL-10, have been shown to exert deleterious effects on pregnancy, inhibiting fetal growth and development . Aim: Measurement of the locally concentrations of selected Th1 and Th2 cytokines in women with a history of recurrent spontaneous abortion (RSA) at the time of abortion using in situ hyperdization technique. Methods: A total of one hundred and nineteen women, ranged from the mean age (23.9 − 28.5)years, were enrolled in the current study and were further classified into three categories: Group A-Recurrent spontaneous abortion (RSA): n= 62 women, with a mean age of (28.5 + 0.68);Group B-non-recurrent spontaneous abortion (non-RSA): n= 34 women, with a mean age of (26.4 ± 0.85)and group C-Control (successful pregnancy): n= 23 women, with a mean age of (23.9 ± 0.88). From each patient and control, placental tissues were collected.

More than 50% of Helicobacter pylori strains produce the toxin CagA which introduced to inside th... more More than 50% of Helicobacter pylori strains produce the toxin CagA
which introduced to inside the gastric mucosal cells. CagA induce upregulation of p53 expression whereas, it plays as pro-apoptotic factor.
Bcl-2 protein is one of the most important members of the family of
anti-apoptosis, hence we aimed to investigate the association between
CagA and Bcl-2. Paraffin embedded sections of gastric tissue from
thirty patients had been included in this study. In addition, 10
apparently healthy volunteers as a control group. Procedure of In Situ
Hybridization was carried out to detect the CagA cytotoxin and
immunohistochemistry to evaluate the expression of Bcl-2. In patients
group 24/30 cases of patients group gave a moderate staining, 5/30
cases gave substantial staining and only 1/30 cases gave slight
staining. the immunoexpression of Bcl-2 in CagA negative cases
(mean 44.706± 3.952 S.E.) was higher than that of CagA positive
cases (mean 40.267± 1.476 S.E.). The CagA positive strain of H.
pylori induce down-regulation of Bcl-2.