Esat Atikkan | Montgomery College (original) (raw)
Uploads
Papers by Esat Atikkan
Cell differentiation, Oct 1, 1977
RNA synthesized in vitro from cl:~romatin~and DNA by calf thymus RNA polymerase III was evaluated... more RNA synthesized in vitro from cl:~romatin~and DNA by calf thymus RNA polymerase III was evaluated by hybridization in vast DNA excess. The RNA contains RNA complementary t o both moderately repeated and unique DNA sequences. Very highly repeated DNA is not transcribed. A greater portion of RNA transcribed from DNA by RNA polymerase III hybridizes to moderately repeated DNA than RNA transcribed by Escherichia col~ RNA polymerase. In studies utilizing DNA absorbed to filters, RNA transcribed from chromatin in short incubations hybridized to a greater extent than RNA transcribed for longer times. Similar results were obtained with RNA transcribed from DNA by E. ~coli RN~I polymerase. These results suggest: 1) RNA polymera~ III may be responsible for the synthesis of RNA species in addition to tRNA and 5 S ribosomal RNA and a portion c.f this RNA is transcribed from unique DNA; and 2) in vitro there may be selectivity in the initiation of transcription by both E. coli RNA polymerase and calf thymus RNA polymerase HI.
Journal of Membrane Biology, Jan 1, 1980
Differentiation and development: …, Jan 1, 1978
Cell differentiation, Oct 1, 1977
RNA synthesized in vitro from cl:~romatin~and DNA by calf thymus RNA polymerase III was evaluated... more RNA synthesized in vitro from cl:~romatin~and DNA by calf thymus RNA polymerase III was evaluated by hybridization in vast DNA excess. The RNA contains RNA complementary t o both moderately repeated and unique DNA sequences. Very highly repeated DNA is not transcribed. A greater portion of RNA transcribed from DNA by RNA polymerase III hybridizes to moderately repeated DNA than RNA transcribed by Escherichia col~ RNA polymerase. In studies utilizing DNA absorbed to filters, RNA transcribed from chromatin in short incubations hybridized to a greater extent than RNA transcribed for longer times. Similar results were obtained with RNA transcribed from DNA by E. ~coli RN~I polymerase. These results suggest: 1) RNA polymera~ III may be responsible for the synthesis of RNA species in addition to tRNA and 5 S ribosomal RNA and a portion c.f this RNA is transcribed from unique DNA; and 2) in vitro there may be selectivity in the initiation of transcription by both E. coli RNA polymerase and calf thymus RNA polymerase HI.
Journal of Membrane Biology, Jan 1, 1980
Differentiation and development: …, Jan 1, 1978